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Choi, Dukhyun,Choi, Min-Yeol,Choi, Won Mook,Shin, Hyeon-Jin,Park, Hyun-Kyu,Seo, Ju-Seok,Park, Jongbong,Yoon, Seon-Mi,Chae, Seung Jin,Lee, Young Hee,Kim, Sang-Woo,Choi, Jae-Young,Lee, Sang Yoon,Kim, Jo WILEY-VCH Verlag 2010 ADVANCED MATERIALS Vol.22 No.19
<B>Graphic Abstract</B> <P>The cover shows an image of fully rollable transparent nanogenerators synthesized using chemical vapor deposition grown large-scale graphene sheets as transparent electrodes and piezoelectric ZnO nanorod arrays. Sang-Woo Kim, Jae-Young Choi, and co-workers report on p. 2187 the electrical and structural stability of the nanogenerators, with excellent charge scavenging performance under external mechanical loads such as bending and rolling. This study shows that graphene-based nanogenerators are very promising for self-powered rollable transparent device applications. <img src='wiley_img_2010/09359648-2010-22-19-ADMA201090066-content.gif' alt='wiley_img_2010/09359648-2010-22-19-ADMA201090066-content'> </P>
S-573 : Depression in patients with SLE and their association with serum BDNF levels
( Sung Hae Chang ),( Ja Hyun Cho ),( Na Hee Shin ),( Hye Jin Oh ),( Myoung Jae Yoon ),( Eun Young Lee ),( Eun Bong Lee ),( Tae Jin Lee ),( Bong Jin Hahm ),( Young Wook Song ) 대한내과학회 2013 대한내과학회 추계학술대회 Vol.2013 No.1
Introduction: Patients with systemic lupus erythematosus (SLE) are vulnerable to depression because of the chronic nature and neuropsychiatric involvement of the disease. Serum brain-derived neurotrophic factor (BDNF) was reported to be decreased in depression. The aim of the study was to investigate the prevalence of depression and its related factors including BDNF in patients with SLE. Material and Methods: One hundred and eighty patients were enrolled at the Rheumatology Clinic from January to March in 2012. The prevalence of depression was assessed using the center for epidemiologic studies depression (CES-D) scale. We evaluated disease activity by physician`s global assessment (PhyGA), patient`s global assessment (PGA), SLE disease activity index (SLEDAI) and disease related organ damage. The EuroQol-5 dimensions (EQ-5D), sociodemographic features and laboratory tests were also surveyed. Serum BDNF was measured using enzyme-linked immunosorbent assay (ELISA). Patients having a CES-D score of 24 or more were considered to have depression. Results: The prevalence of depression in SLE was 22.8% (n=41). In univariate analysis, unmarried/disrupted marital status (patients with single/divorced/estrangement/bereavement status than those with married status), unemployment, low annual income, higher PGA, higher PhyGA, lower EQ-5D index score and severe pain/discomfort were significantly associated with depression. In multivariate analysis, unmarried/disrupted marital status, higher PGA and severe pain/discomfort were significantly associated with depression. Serum BDNF levels were not associated with depression (p=0.62) but negatively correlated with disease activity in patients with SLE (r=-0.188, p=0.021). Conclusion: Depression is prevalent in patients with SLE and is associated with unmarried/disrupted marital status, severe pain/discomfort and higher PGA. Serum levels of BDNF were not associated with depression but were negatively correlated with disease activity. Treatment of depression may be beneficial in patients with severe pain/discomfort or high PGA.
Sung, Eun-Sil,Park, Kyung-Jin,Lee, Seung-Hyun,Jang, Yoon-Seon,Park, Sang-Koo,Park, Yoo-Hoi,Kwag, Won-Jae,Kwon, Myung-Hee,Kim, Yong-Sung American Association for Cancer Research, Inc 2009 Molecular Cancer Therapeutics Vol.8 No.8
<P>The proapoptotic tumor necrosis factor-related apoptosis inducing ligand (TRAIL) receptors death receptor (DR) 4 and DR5 are attractive targets to develop the receptor-specific agonistic monoclonal antibodies (mAb) as anticancer agents because of their tumor-selective cell death-inducing activity. Here, we report a novel agonistic mAb, AY4, raised against human DR4 in mice. ELISA analysis revealed that AY4 specifically bound to DR4 without competition with TRAIL for the binding. Despite distinct binding regions of AY4 on DR4 from those of TRAIL, AY4 as a single agent induced caspase-dependent apoptotic cell death of several tumor types through the extrinsic and/or intrinsic pathways without substantial cytotoxicity to normal human hepatocytes. Further, the AY4-sensitive cells followed the same cell death characteristics classified as type I and type II cells by the response to TRAIL, suggesting that the cell death profiles in responses to DR4 and/or DR5 stimulation are determined by the downstream signaling of the receptor rather than the kind of receptor. Noticeably, AY4 efficiently induced cell death of Jurkat cells, which have been reported to be resistant to other anti-DR4 agonistic mAbs, most likely due to the unique epitope property of AY4. In vivo administration of AY4 significantly inhibited tumor growth of human non-small cell lung carcinoma preestablished in athymic nude mice. Conclusively, our results provide further insight into the DR4-mediated cell death signaling and potential use of AY4 mAb as an anticancer therapeutic agent, particularly for DR4-responsive tumor types.</P>
( Hyun Yang ),( Pil Soo Sung ),( Jae Jun Lee ),( Soon Kyu Lee ),( Hee Chul Nam ),( Jeong Won Jang ),( Jong Young Choi ),( Seung Kew Yoon ),( Eun Sun Jung ),( Si Hyun Bae ) 대한간학회 2020 춘·추계 학술대회 (KASL) Vol.2020 No.1
Aims: Drug-induced liver injury (DILI) is caused by the interplay between drugs, their metabolites and host immune response. The aim of this study is to investigate the phenotypes of the infiltrating immune cells in DILI and the role of steroid treatment in DILI. Methods: From January 2017 to February 2020, 41 consecutive patients with DILI who underwent liver biopsy were enrolled prospectively in this study. Diagnosis of DILI was based on patient medication history after exclusion of the other etiologies. Liver biopsy was performed, and immunohistochemical stain and multicolor fluorescence-activated cell sorting (FACS) analysis were done with the biopsy specimen. Experienced pathologist confirmed the pathologic and immunohistochemical findings. Results: Median RUCAM score was 9 (5-14). Patients with positive for anti-nuclear antibody, smooth muscle antibody, and liver-kidney microsomal antibody were 28 (70%), 3 (7.5%), and 0, respectively. The number of intrahepatic T cells (CD3+ cells) showed positive correlation with serum levels of total bilirubin, AST, ALT, and model for end-stage liver disease (MELD) score (r=0.353, 0.353, 0.381, and 0.352, respectively, P<0.05). The number of intrahepatic macrophages (CD68+ cells), also showed positive correlation with serum levels of total bilirubin, AST, ALT, and MELD score (r=0.441, 0.508, 0.505, and 0.404, respectively, P<0.05). The frequency of activated CD8+ T cells (CD38+HLA-DR+) among the liver-infiltrating CD8+ T cells in DILI livers, was significantly higher than that in healthy livers (P<0.01). Importantly, the percentage of CD38+HLA-DR+ cells among intrahepatic CD8+ T cells in DILI livers showed positive correlation with ALT (r=0.593, P=0.04). Thirty patients (73.2%) were treated with steroid. Among them, 22 patients (78.6%) showed more than 50% of reduction of ALT level after 1 week of steroid treatment and 2 patients progressed to hepatic failure, but recovered after steroid treatment. Conclusions: In conclusion, we found the positive correlation between the number of intrahepatic macrophages, T cells, CD8+ T cells and activated CD8+ T cells infiltrations and the degree of liver injury in patients with DILI. We suggest that T cells and macrophage play critical roles in DILI. Therefore, steroid can be a treatment option for patient with DILI. This study was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (2020R1A2C3011569). This study was also supported by the Research Fund from Eunpyeong St. Mary’s Hospital.
4-Hydroxynonenal Induces Endothelial Cell Apoptosis via ROS and Peroxynitrite Generation
Sang Woon Chung(정상운),Su Bog Yee(이수복),Ji Young Lee(이지영),Mohammad Akbar Hossain(호세인 모하메드 악바르),Dong Hwan Kim(김동환),Jeong-Hyun Yoon(윤정현),Hae Young Chung(장정윤),Nam Deuk Kim(김남득) 한국생명과학회 2011 생명과학회지 Vol.21 No.7
지질과산화로부터 생성된 aldehyde 중 4-hydroxynonenal (HNE)는 산화적 손상과 관련된 다량의 arachidonic acids, linoleic acids 등으로부터 생성될 수 있다. 그러므로 HNE는 산화적 스트레스와 관련된 세포사에서 중요한 매개인자로 작용을 할 수가 있을 것이다. 본 연구는 HNE가 세포사를 유발할 것이라 가정하고, 먼저 흰쥐 전립선 유래 내피세포인 YPEN-1 세포에서 세포독성을 측정하였다. 세포생장 저해능력은 HNE를 5~15 μM 농도로 처리하여 형태적 변화와 MTT assay를 통하여 결과를 관찰하였다. 그 결과 HNE가 이 세포에서 핵형의 변화와 세포사를 유발시키는 것을 각각의 실험을 통해 확인이 되었다. 또한 이 사실을 단백질의 변화를 통하여 확인을 할 수가 있었다. HNE를 24시간 처리한 세포에서 poly(ADP-ribose) polymerase 단백질 분절이 매개되었고 Bax의 발현량이 증가하였다. 또한 세포내의 활성 산소종들을 발생시켰다. 이에 생성된 활성 산소종과 peroxynitrite가 세포사와 관련이 있는가를 밝히기 위하여 이들의 포식자들인 N-acetylcysteine과 penicillamine을 본 연구에서 사용하였다. 이들 포식자들에 의해 HNE에 의해 유도되는 세포사가 억제가 되었기에 산화적 활성화가 HNE에 의해 유도된 세포사와 관련이 있음을 알 수 있었다. 이러한 결과들은 HNE가 내피세포에서 ROS와 peroxynitrite 생성을 통하여 세포사를 일으킨다는 사실을 뒷받침해 준다. The formation of reactive lipid aldehydes, 4-hydroxynonenal (HNE) is shown to be derived from fatty acid hydroperoxides through the oxidative process. Among its known effects in cytotoxicity, HNE has been implicated in apoptotic cell death. To delineate its putative role as a potential mediator, we investigated the mechanism by which HNE induces apoptosis of endothelial cells (ECs). The anti-proliferative effects of HNE were tested through MTT assay after exposure to various concentrations (5~15 μM) of HNE. We observed apoptotic bodies with propidium iodide staining, and measured the HNE induction of endothelial apoptosis by flow cytometry assay. We observed that cells exposed to HNE for 24 hr resulted in increased poly(ADP-ribose) polymerase cleavage and up-regulation of Bax. Data on the HNE action strongly indicated the involvement of reactive species, namely, intracellular ROS, nitrite, and peroxynitrite. To obtain evidence on the implication of ROS and peroxynitrite in HNE-induced apoptosis, a ROS scavenger, N-acetylcysteine (NAC), and a peroxynitrite scavenger, penicillamine, were tested. Results clearly indicate that the induction of apoptosis by HNE was effectively inhibited by NAC and penicillamine. Based on the present data, we conclude that the endothelial apoptosis induced by HNE involves both ROS generation and peroxynitrite activity. Our new data could lead to a redefinition of HNE action on apoptosis in ECs.
Yoon, Ok J.,Kim, Hyun W.,Kim, Duck J.,Lee, Hyun J.,Yun, Ji Y.,Noh, Yoo H.,Lee, Do Y.,Kim, Do H.,Kim, Sung S.,Lee, Nae-Eung WILEY-VCH Verlag 2009 Plasma Processes and Polymers Vol.6 No.2
<P>The conductance of amine-functionalized SWCNTs (a-SWCNTs), obtained by inductively coupled N<SUB>2</SUB>/H<SUB>2</SUB> plasma treatment of commercially available carboxyl-functionalized SWCNTs (c-SWCNTs), was decreased compared to that of the c-SWCNTs. As a result, the diameter and surface energy of electrospun PLGA/a-SWCNT nanocomposites decreased and increased, respectively, compared to those of electrospun PLGA/c-SWCNT nanocomposites. The results also showed that the a-SWCNTs were well dispersed in the nanocomposites, improved adhesion of SWCNTs to the surrounding polymer matrix and thereby enhanced the tensile modulus of electrospun PLGA/c-SWCNT and PLGA/a-SWCNT nanocomposites by 127 and 226%, respectively, compared to that of electrospun PLGA membranes.</P><P> <img src='wiley_img/16128850-2009-6-2-PPAP200800081-gra001.gif' alt='wiley_img/16128850-2009-6-2-PPAP200800081-gra001'> </P>
Lyn inhibits osteoclast differentiation by interfering with PLCγ1-mediated Ca<sup>2+</sup> signaling
Yoon, Soo-Hyun,Lee, Youngkyun,Kim, Hyung-Joon,Lee, Zang Hee,Hyung, Seok-Won,Lee, Sang-Won,Kim, Hong-Hee Elsevier 2009 FEBS letters Vol.583 No.7
<P><B>Abstract</B></P><P>Osteoclasts differentiate from macrophage-lineage cells to become specialized for bone resorption function. By a proteomics approach, we found that Lyn was down-regulated by the osteoclast differentiation factor, receptor activator of NF-κB ligand (RANKL). The forced reduction of Lyn caused a striking increase in the RANKL-induced PLCγ1, Ca<SUP>2+</SUP>, and NFATc1 responses during differentiation. These data suggest that Lyn plays a negative role in osteoclastogenesis by interfering with the PLCγ1-mediated Ca<SUP>2+</SUP> signaling that leads to NFATc1 activation. Consistent with the in vitro results, in vivo injection of Lyn specific siRNA into mice calvariae provoked a fulminant bone resorption. Our study provides the first evidence of the involvement of Lyn in the negative regulation of osteoclastogenesis by RANKL.</P>