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Huh, Yoon Sun,Lee, Joung Kwan,Kim, Ik Jei,Kang, Bo Goo,Lee, Ki Yeol The Korean Society of Plant Biotechnology 2015 식물생명공학회지 Vol.42 No.2
Interest and great demand for blueberry (Vaccinium corymbosum) have increased, as V. corymbosum is now one of the most economically important crops in Korea. It is expected that blueberry production and the area planted for cultivation will increase consistently in the years ahead because of high profitability and the consumer's demand for healthy ingredients. Effective mass production of blueberry is urgently needed for commercial cultivation establishment, but a main limitation is lack of a propagation system that produces a disease-free plant material for commercial plantation. A large amount of research has focused entirely on developing tissue culture techniques for blueberry propagation. However, controlling fungal and bacterial contamination of woody plant material is extremely difficult. Our study was conducted to investigate the effect of biocide addition during the in vitro culture of blueberry on plantlet growth and contamination occurrence. Four biocides, including Plant Preservative Mixture ($PPM^{TM}$), vancomycin, nystatin and penicillin G, were used in varying concentrations during the in vitro propagation of blueberry. When nystatin was added into the medium at low concentrations, the overall growth of blueberry plantlets was retarded. Addition of vancomycin and penicillin G in high concentrations decreased contamination but induced plantlet mortality. On the other hand, when 1ml/L $PPM^{TM}$ was added, the growth characteristics of blueberry plantlets did not significantly differ from non-treatment (control), and the contamination occurrence rate was very low. From these results, we found that the addition of the appropriate biocide could provide an effective method to reduce contamination in the culture process, thereby raising in vitro production efficiency.
Yoon-Sun Huh(허윤선),Eun-Jeong Kim(김은정),Sol-Ji Noh(노솔지),Yu-Min Jeon(전유민),Sung-Won Park (박성원),Geon-Sig Yun(윤건식),Tae-Il Kim(김태일),Young-Ho Kim(김영호) (사)한국생물환경조절학회 2020 생물환경조절학회지 Vol.29 No.4
본 연구는 시설 내 소형 수박 재배 시 관수개시점에 따른 토양수분 함량별 생육, 수량 및 생리적 반응 특성의 차이를 구명하고 소형 수박 생산에 유리한 관수조건을 구명하고자 수행하였다. 토양수분 센서를 이용하여 정식 후 14일부터 수확 7 ~ 10일 전까지 관수개시점별 5처리(-10, -20, -30, -40, 50 kPa)를 두어 관수하였다. 토양수분 함량이 가장 낮은 개시점-50 kPa 처리에서 전반적인 지상부 생육특성은 저조하였으나, 근장 및 뿌리 건물율은 증가하였다. 광합성률, 기공전도도 및 증산율 비교 시, 관수개시점-50 kPa 처리에서 가장 낮았고, -20 kPa ~ -40 kPa 처리 시 광합성률은 높게 조사되었다. 착과율 및 총 상품수량은 -30 kPa 및 -40 kPa 처리에서 각각 84.7 ~ 85.5%, 5,144 ~ 5,305 kg/10a으로 유의하게 증가하였다. 식물체의 외부환경 관련 스트레스 지표 물질로 알려진 프롤린, ABA, 총 페놀 및 시트룰린의 함량은 토양수분 함량이 낮아질수록 증가하였으며, 특히 관수개시점-50 kPa 처리에서 가장 높게 조사되었다. 따라서 이와 같은 결과를 종합해 볼 때, 시설 내 안정적인 소형 수박 생산을 위하여 관수개시점을 -30 kPa ~ -40 kPa 수준으로 조정하여 토양수분 함량을 조절하는 것이 수박 생육 향상 및 상품수량 증대에 가장 유리한 것으로 판단되었다. Watermelon yield mainly depends on soil water content controlled by irrigation in a plastic greenhouse. In this study, we investigated the effect of different soil moisture contents affected by irrigation starting point on growth, yield, and physiological responses of small-sized watermelons. Irrigation was initiated at 5 different levels of soil water content as a starting point with soil moisture detecting sensor after 14 days of transplanting, and stopped at 7 ~ 10 days before harvest. These treatments were compared with the conventional periodic irrigation as control. When soil had the lowest moisture content (-50 kPa), the overall shoot growth was retarded, but the root length and root dry weight increased. The photosynthetic parameters (photosynthetic rate, stomatal conductance, and transpiration rate) of watermelon leaves decreased significantly in the lowest soil moisture content (-50 kPa). On the other hand, the photosynthetic rates of watermelon leaves grown with irrigation starting point between -20 and -40 kPa were observed to be higher than those of other treatments. Fruit set rate and marketable fruit yield increased significantly at –30 kPa and –40 kPa. Proline, abscisic acid (ABA), total phenol and citrulline, which are known to contribute to stress tolerance under drought condition, increased as soil water content decreased, particularly, the largest increases were recorded at –50 kPa. From these results, it was found that an appropriate water supply adjusted with an irrigation starting point between -30 and -40 kPa could help to keep favorable soil water content during the cultivation of small-sized watermelons, promoting the marketable fruit production as well as inducing the vigorous plant growth and reproductive development.