돼지에서 대장균 자가백신 효과

윤교복 ( Kyo Bok Yoon ),김종술 ( Jong Sool Kim ),정동수 ( Dong Su Chung ),박양주 ( Young Joo Park ),이유섭 ( You Sub Lee ),한정희 ( Jeong Hee Han ) 한국가축위생학회 1999 韓國家畜衛生學會誌 Vol.21 No.2

The present study was carried out to investigate the prevalence of brucellosis in Kyungbuk area for the 3 years from 1966 to 1998. Collective milk samples were routinely screened to detect positive farms by using the milk ring test(MRT), and serum agglutination test was performed to detect sero-positive individuals in the MRT positive farms. Attempt were made to isolate the causative organismas from slaughtered sero-positive reactors and some biochemical and polymerase chain reation characters of the isolates were also made to identify the organisms. Seroprevalence to brucellosis in peoples who are close contact with infected dairy herds was also investigated. Brucellosis of dairy cattle was rare before 1997, but has been broken more frequently since early 1998. By the MRT for dairy herds, positive rate was gradually increased every year 0.6% in 1996, 1.5% in 1997, 3.9% in 1998. Among 262 MRT-positive herds, only 21 herds(8.0%) showed positive brucellosis in serological test. The isolation rates of Brucella sp from tested materials were 51.2% in supramammary glands, 39.5% in milks, and 50.0% in pulmonary lymphnode, respectively. Isolated strain and biotype were Brucella(B) arbortus biotype 1 in 26 heads, and were B suis biotype 1 in 2 heads. Isolated strain and vaccine strain were very similar in their colony morphology and staining. In drug susceptibility, isolated stains(B abortus) and vaccine strain(B abortus RB-51) were sensitive to ampicillin, gentamycin, kanamycin, neomycin, penicillin, streptomycin, and tetracycline, but resistant to erythromycin. In the PCR, field strains reacted to BA and 1S711 primers, and vaccine strain reacted to BA, 1S711, and RB51 primers. In the plate agglutination test of 96 sera of human contacted with animals, serum antibody titer detected 1: 100 in one person, 1 : 200 in one, and below 1 : 25 in the others.

흉부 X-선 사진을 이용한 성인의 기관-기관지 각

채윤정,임현교,김순열,이광호,최재찬,윤경봉,이영복 연세대학교 원주의과대학 2000 원주의대논문집 Vol.13 No.1

Osteogenic Differentiation of Human Adipose-Derived Stem Cells(hADSCs) on a Dexamethasone Eluting Nanofiber Scaffolds

( Jung Bok Lee ),( Sung Min Jeong ),( Kyoung Jun Kim ),( Dong Hyun Cho ),( Il Keun Kwon ),( In Chan Yoon ),( Kui Won Choi ),( Jun Kyo Francis Suh ),( Jae Hong Park ),( Yong Duk Park ),( Jong Hyuk Chun 한국조직공학·재생의학회 2009 조직공학과 재생의학 Vol.6 No.1

Dexamethasone(DEX), a synthetic steroidal anti-inflammatory drug, is an efficient and dependable drug that induces osteogenic differentiation. The aim of this study is to fabricate the DEX loaded PLGA nanofibers by electrospinning method. Adipose-derived stem cells(ADSCs) were seeded into the nanofiber and the sustained release of DEX from PLGA nanofiber scaffolds promoted their osteogenic differentiation. The properties of DEX loaded PLGA nanofiber scaffold were characterized by scanning electron microscopy(SEM) and the release kinetics of DEX from PLGA nanofibers in vitro(1 h to 14 days) was evaluated by high performed liquid chromatography(HPLC). To evaluate the cellular response of the ADSCs seeded onto DEX loaded PLGA nanofiber, we performed F-actin, cytotoxicity, alkaline phosphatase activity, alizarin red S, von Kossa staining and immunocytochemistry assays for osteogenic differentiation. DEX loaded PLGA nanofiber scaffold was observed to have sustained release in vitro during experimental periods. The cytotoxicity test of the DEX loaded PLGA nanofiber scaffold indicated there was almost no-toxic effects in regards to proliferation and differentiation of adiposederived stem cells(ADSCs) as compared with a control. Alkaline phosphatase activity and alizarin red S were more significantly increased after 14days with increased DEX concentration of PLGA nanofiber scaffold than with PLGA nanofiber only. Also, von Kossa staining results confirmed a larger area of calcium deposition with increased DEX concentration of PLGA nanofiber scaffold. We observed that osteocalcin was also increased with increasing DEX concentration. These results demonstrate that DEX acts as an osteogenic inductive factor, and increased DEX concentration promoted more osteogenic differentiation of ADSCs. In conclusion, DEX loaded PLGA nanofiber would be valuable tool for bone tissue regeneration.

Mechanically reinforced cell-laden scaffolds formed using alginate-based bioink printed onto the surface of a PCL/alginate mesh structure for regeneration of hard tissue

Kim, Yong Bok,Lee, Hyeongjin,Yang, Gi-Hoon,Choi, Chang Hyun,Lee, DaeWeon,Hwang, Heon,Jung, Won-Kyo,Yoon, Hyeon,Kim, Geun Hyung Elsevier 2016 JOURNAL OF COLLOID AND INTERFACE SCIENCE - Vol.461 No.-

<P><B>Abstract</B></P> <P>Cell-printing technology has provided a new paradigm for biofabrication, with potential to overcome several shortcomings of conventional scaffold-based tissue regeneration strategies via controlled delivery of various cell types in well-defined target regions. Here we describe a cell-printing method to obtain mechanically reinforced multi-layered cell-embedded scaffolds, formed of micron-scale poly(ε-caprolactone) (PCL)/alginate struts coated with alginate-based bioink. To compare the physical and cellular activities, we used a scaffold composed of pure alginate (without cells) coated PCL/alginate struts as a control. We systematically varied the ratio of alginate cross-linking agent, and determined the optimal cell-coating conditions to form the PCL/alginate struts. Following fabrication of the cell (MG63)-laden PCL/alginate scaffold, the bioactivity was evaluated in vitro. The laden cells exhibited a substantially more developed cytoskeleton compared with those on a control scaffold consisting of the same material composition. Based on these results, the printed cells exhibited a significantly more homogenous distribution within the scaffold compared with the control. Cell proliferation was determined via MTT assays at 1, 3, 7, and 14days of culture, and the proliferation of the cell-printed scaffold was substantially in excess (∼2.4-fold) of that on the control. Furthermore, the osteogenic activity such as ALP was measured, and the cell-laden scaffold exhibited significantly greater activity (∼3.2-fold) compared with the control scaffold.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

이동 경로 데이터에 기반한 이동 객체의 시공간 위치 예측 기법

윤태복(Tae Bok Yoon),박교현(Kyo Hyun Park),이지형(Jee Hyong Lee) 한국지능시스템학회 2006 한국지능시스템학회논문지 Vol.16 No.5

사용자에게 적응된 서비스를 제공하기 위하여 환경으로부터 얻어지는 다양한 형태의 데이터를 이용한 다양한 방법이 연구되고 있다. 그 중 과거 이동 경로 자료는 사용자의 현재 이동 위치를 예측하고 이와 관련된 서비스를 제공하는데 유용하게 사용될 수 있다. 본 논문에서는 사용자의 과거 이동 경로의 분석을 통하여 이동중인 사용자의 시공간 위치예측 기술을 제안한다. 환경으로부터 발생한 사용자의 이동 경로를 수집하고, 수집된 데이터에서 이동 경로 선택(Path Selection) 방법을 이용한다. 이동 경로 선택 방법은 이동 중에 발생한 경로의 거리, 시간, 방향의 요소와 동적정합법을 사용하여 유사성(Similarity)을 측정하며 유사성이 가장 높은 경로를 선택한다. 선택된 경로는 시간에 따른 공간 정보 및 위치에 따른 시간 예측 서비스를 위하여 사용가능 하며, 실험을 통하여 유사성이 높은 이동 경로를 선택하는 모습을 확인하였다. User adaptive services have been important features in many applications. To provide such services, various techniques with various kinds of data are being used. In this paper, we propose a method to analyze user's past moving paths and predict the goal position and the path to the goal by observing the user's current moving path. We develop a spatiotemporal similarity measure between paths. We choose a past path which is the most similar to the current path using the similarity. Based on the chosen path, user's spatiotemporal position is estimated. Through experiments we confirm this method is useful and effective.

개흉술후 통증관리를 위한 흉부경막외강 Morphine-Bupivacaine 의 지속적 주입의 효과

이영복,임현교,윤경봉,임영수,김훈도 대한마취과학회 1998 Korean Journal of Anesthesiology Vol.34 No.2

Background : The purpose of this study was to evaluate the analgesic effect and side effects including respiratory depression and cardiovascular changes after continuous high thoracic epidural infusion of morphine when given with bupivacaine for postthoracotomy pain. Methods : In a prospective study, 25 patients who received continuous thoracic epidural administration of morphine with bupivacaine after thoracotomy were studied. The analgesia was provided by a continuous epidural infusion for 2 days. Initially, 0.25% bupivacaine 5∼7 ml with morphine 1 mg was injected epidurally. Then, continuous epidural infusion of 0.25% bupivacaine 2 ml/hr with morphine 0.125 mg/hr was followed. Results : The pain score(visual analogue pain scale) was below 3 at all postoperative periods except postoperative 1 hour with cough. The significant PaCO2 and cardiovascular changes for 2 days after epidural injection were not occured. There were no significant side effects requiring treatment. Conclusion : Continuous high thoracic epidural administration of 0.125 mg/hr of morphine with 0.25% bupivacaine is safe and effective analgesic method for thoracotomy. (Korean J Anesthesiol 1998; 34: 371∼375)

Hemodynamic Effect of Pulmonary Artery Ligation during Pneumonectomy

Kwang Ho Lee,Hyun Kyo Lim,Eun Sung Jun,Young Bok Lee,Kyung Bong Yoon,Jae Chan Choi,Soon Yul Kim,Ryung Choi Korean Society of Critical Care Medicine 2000 Acute and Critical Care Vol.15 No.2

BACKGROUND: Pulmonary artery ligation during pneumonectomy increase the pulmonary blood flow of dependent lung and may increase the pulmonary arterial pressure and pulmonary vascular resistance. The purpose of this study is to evaluate the hemodynamic effect of pulmonary artery ligation during pneumonectomy. METHODS: Nine patients who were supposed to receive pneumonectomy were studied. Hemodynamic measurements were performed following two lung ventilation (TLV), one lung ventilation (OLV), after pulmonary artery ligation and after pneumonectomy. RESULTS: There is no significant differences in heart rate, systemic arterial pressure, pulmonary arterial pressure, pulmonary capillary wedge pressure, cardiac index and pulmonary vascular resistance index. Arterial oxygen tension significantly reduced during OLV and increased after pulmonary artery ligation and after pneumonectomy. CONCLUSIONS: These results suggest that pulmonary artery ligation during pneumonectomy may not affect the cardiopulmonary hemodynamics.

Brucellosis outbreak of Korean indigenous cattle at Yeongwol and Pyeongchang county in Korea

Kim Song-Tae,Yoon Kyo-Bok,Kang Tae-Kyu,Bak Won-Hern,Lee Jung-Hyun,Chung Dong-Su The Korean Society of Veterinary Service 2005 韓國家畜衛生學會誌 Vol.28 No.4

This study was attempted to investigate the properties of brucellosis in Korean indigenous cattle at the Yeongwol and Pyeongchang county. Brucella spp was differentiated and identified from cotyledons, amniotic fluids and supramammary lymph nodes which confirmed with clinical, serological, epidemiological evidences (69 cases) from January to June, 2004. Isolation frequency of this causative agent from supramammary lymph nodes, cotyledons and amniotic fluids from 38 pregnant Korean indigenous cattle were $39.1\%,\;87.5\%,\;and\;63.2\%$, respectively, and finally confirmed with Brucella abortus biotype 1 through biochemical and serological test. A Brucella specific DNA with 711bp band was detected by PCR assay using BCSP primer. The two cases were definite epidemiological evidences that infected Korean indigenous cattle acrossed the border to Yeongwol and Pyeongchang from near two provinces. Effective prevention programs are urgently needed for further spreading this epidemics.

Brucellosis outbreak of Korean indigenous cattle at Yeongwol and Pyeongchang county in Korea

( Song Tae Kim ),( Kyo Bok Yoon ),( Tae Kyu Kang ),( Won Hern Bak ),( Jung Hyun Lee ),( Dong Su Chung ) 한국가축위생학회 2005 韓國家畜衛生學會誌 Vol.28 No.4

This study was attempted to investigate the properties of brucellosis in Korean indigenous cattle at the Yeongwol and Pyeongchang county. Brucella spp was differentiated and identified from cotyledons, amniotic fluids and supramammary lymph nodes which confirmed with clinical, serological, epidemiological evidences(69 cases) from January to June, 2004. Isolation frequency of this causative agent from supramammary lymph nodes, cotyledons and amniotic fluids from 38 pregnant Korean indigenous cattle were 39.1%, 87.5%, and 63.2%, respectively, and finally confirmed with Brucella abortus biotype 1 through biochemical and serological test. A Brucella specific DNA with 711bp band was detected by PCR assay using BCSP primer. The two cases were definite epidemiological evidences that infected Korean indigenous cattle acrossed the border to Yeongwol and Pyeongchang from near two provinces. Effective prevention programs are urgently needed for further spreading this epidemics.

개에서 공기색전 유발후 100% O₂ 및 50% O₂-50% N₂O를 사용한 폐환기가 혈역학 변화에 미치는 영향 비교

이광호,윤경봉,임현교,이영복,김순열,최재찬,오중환 연세대학교 원주의과대학 2000 원주의대논문집 Vol.13 No.1