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임효택,이계윤 한국스포츠리서치 2001 한국 스포츠 리서치 Vol.12 No.4
The purpose of this study was to analyze and determine the relationships of the users of Sport and leasure facilities with factors related to commercial sport and leasure products(the interests in sport and leasure products, the cognizance of sport and leasure products, the cognizance of the brands and the cognizance of the quality). 700 subjects answered questionnaires. The result were as follows : 1. In comparison of sex with the factors, there were significant differences in all factors excepting the factor of quality. Male had more interests in sport and leasure products and had more cognizance of brands than female. Female had more cognizance of products than male. 2. In comparison of age with the factors, there were significant differences in all factors excepting the cognizance of the products. Generally, regardless of age, the interests and cognizance of quality were higher than the other two factors. 3. In comparison of marital status with the factors, there were no significant differences excepting the interests. the married had more interests than the unmarried. 4. In comparison of formal schooling with the factors, there were significant differences in all factors excepting the cognizance of the products. 5. In comparison of monthly income with the factors, there were significant differences in all factors excepting the cognizance of the products. In general, the more monthly income, the more interests in and the more cognizance of brands and quality of the products. 6. In comparison of the events of sport and leasure with the factors, there were significant differences in all factors excepting the cognizance of the quality of the products. In comparison of activity levels, there was significant difference only in the cognizance of the brands. 7. In the events of sport and leasure, the highest interest in the products was observed in golf while the highest cognizance of products was observed in squash and racket-ball. The highest cognizance of the brands of the products was observed in bowling. In case of participating in the activities once a week, the highest cognizance of the brands was observed. 8. Sex was significantly related to satisfaction with goods for sport and leasure. According to formal schooling and monthly income, there were significant differences in the satisfaction with the products. 9. According to the events of sport and leasure and the activity levels, there were significant differences in the satisfaction with the products. The highest satisfaction was observed in squash and racket-ball. while the lowest satisfaction was observed in bowling and aerobic dance. In case of participating in the activities every day, the highest satisfaction was observed.
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일 도농복합지역 저소득층 노인의 건강문제 분석 : 보건소 방문간호사업 대상자 중심으로
고일선,,이태화,이경자,이정렬,임미혜,천의영,주윤미,이계철 노인간호학회 2005 노인간호학회지 Vol.7 No.2
Purpose: The purpose of this study was to analyze the health problems of low-income aged with chronic illness living in urban-rural composite area. Method: The sample consisted of 440 aged who were receiving home care services from a public health center. MDS-HC was applied to analyze the health problems of the aged. Data were collected through a face- to-face interview by six trained interviewers from June 28 to July 15, 2004. Result: Subjects had average number of 8 health problems in both urban and rural area. Lack of preventive health care measure, pain, and visual function were the most frequent health problem. The rural aged had more pain, bowel management problems, compared to the urban aged having more urinary incontinence and indwelling catheter. There were many health problems related to falls and pressure ulcers with middle-old aged in urban, and old-old aged in rural area. Conclusion: The results of this study showed strategies for care intervention of low-income elderly to put in practice. Therefore, tailored-service for each subject should be provided.
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Hf 가 첨가된 Fe-30at.%A1-5at.%Cr 합금의 미세조직 및 열간압연가공 특성
윤계림(Kye Lim Yoon),이도재(Doh Jae Lee),백대화(Dae Hwa Baek),이경구(Kyung Ku Lee) 한국주조공학회 2001 한국주조공학회지 Vol.21 No.6
N/A This study was carried out to examine the effects of adding 0.3at.%Hf in Fe-30at.%Al-5at.%Cr alloy on the variation of microstructures and hot workability. The effect of hot rolling on mechanical properties was estimated by measuring the elongation and tensile strength after rolling at 800 and 1000 respectively. Microstructure of Fe-30at.%Al-5at.%Cr alloy was consisted of large equiaxed grains and it was changed to quasi-equiaxed or columnar structures by adding 0.3at.%Hf to Fe-30at.%Al-5at.%Cr alloy. Every specimens showed a decreased tensile strength after hot rolling compared to that of before rolling. The elongation was increased by hot rolling. Remarkable changes in elongation by hot rollong was observed such as from 1.4% to 4.5% elongation at the specimen of 0.3at.%Hf added to Fe-30at.%Al-5at.%Cr. Fe-30at.%Al-5at.%Cr alloy showed typical cleavage fracture on tensile failure and hot rolling has negligible effects on fracture mode in this alloy. However at the alloy containing Hf fracturemode was changed by hot rolling from intergranular to mixed intergranular and transgranular fracture mode.
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Yoon, Kyung-Lim,Ko, Jin-Hee,Shim, Kye-Shik,Han, Mi-Young,Cha, Sung-Ho,Kim, Su-Kang,Jung, Joo-Ho The Korean Pediatric Society 2011 Clinical and Experimental Pediatrics (CEP) Vol.54 No.8
Purpose: Hyperhomocysteinemia is known as a risk factor for atherosclerosis. Preclinical arteriosclerosis is noted and premature atherosclerosis is known to be accelerated in Kawasaki disease (KD) patients. Genetic polymorphisms in the 5,10-methylenetetrahydrofolate reductase (MTHFR) gene result in elevated plasma homocysteine concentrations and are known to be associated with the development of coronary artery disease. Our hypothesis is that single nucleotide polymorphisms (SNPs) of the MTHFR gene are related to the development of KD and coronary artery lesions (CALs). Methods: For this study, we selected 3 candidate single nucleotide polymorphisms (SNPs) (rs2274976, rs1801131, and rs1801133) of MTHFR. These SNPs are located on chromosome 1p36.3. We included 101 KD patients and 306 healthy adults as controls in this study. CALs were seen in 38 patients. Genotypes of the selected SNPs were determined by direct sequencing and analyzed with SNPAlyze. Results: The genetic distribution and allelic frequency of the 3 MTHFR SNPs (rs2274976, rs1801131, and rs1801133) were not significantly different in patients with KD compared to the control group (P=0.71, 0.17, and 0.96, respectively). There was no difference in the genetic distribution of the MTHFR SNPs between the normal control group and the CAL group (P=0.43, 0.39, 0.52 respectively). Conclusion: The genetic distribution of the MTHFR SNPs (rs2274976, rs1801131, and rs1801133) was not different in the KD group compared to the control group. In addition, the genetic distribution of these SNPs was not different in the CAL group compared to the control group in the Korean population.
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Yoon-Kyu Lim,Seong-Hee Kim,Min-Keun Park,Jin-Young Kim,Pham Duc Chuong,Yong- Soon Lee,윤병수,Kyu-Kye Hwang 대한수의학회 2005 Journal of Veterinary Science Vol.6 No.1
Five monoclonal antibodies (MAbs) and chicken immunoglobulin (IgY) were developed by immunizing with flagella purified from Listeria monocytogenes 4b and the five MAbs have been confirmed to be specific against three different epitopes of flagellin. The antibodies showed specific reaction to Listeria genus and no cross-reactivity with other bacteria tested in this experiment including E.coli O157:H7 and Salmonella enteritidis. Sandwich enzyme-linked immunosorbent assays (ELISA) using the MAbs and IgY were developed to detect Listeria species and the sensitivity and specificity of the developed ELISA have been analyzed. The detection limit of ELISA using MAb 2B1 and HRP labeled IgY was 1 × 105 cells/0.1 ml at 22oC and 1 × 106 cells/0.1 ml at 30oC. ELISA using the pair of MAbs (MAbs 2B1 and HRP labeled MAbs 7A3) detected up to 104 cells/0.1 ml at 22oC and 30oC. Detection limit of sandwich ELISA using IgY was 10 times lower than MAb pair. Using the developed ELISA, we could detect several Listeria contaminated in food samples after 48 h-culturing. In conclusion, both MAbs and IgY have been proved to be highly specific to detect Listeria flagella and the developed sandwich ELISA using these antibodies would be useful tool for screening Listeria spp. in food.
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Kye-Hoon Kim,Young-Nam Kim,Ga Hee Lim,Mi Na Lee,Yoon-Hwa Jung 한국토양비료학회 2011 한국토양비료학회지 Vol.44 No.1
The MuSI is known as a multiple stress resistant gene with several lines. A previous study using RT-PCR showed that the expression of MuSI gene in tobacco plant induced its tolerance to Cd stress. This study was conducted to examine the enhanced Cd tolerance of the MuSI transgenic tobacco plant through germination test and to understand the role of the involved antioxidant enzymes for the exhibited tolerance. Germination rate of MuSI transgenic tobacco was more than 10% higher than that of wild-type tobacco, and seedlings of MuSI transgenic tobacco grew up to 1.6 times larger and greener than seedlings of wild-type tobacco at 200 and 300 μM Cd. From the third to the fifth day, CAT activities at 100 and 200 μM Cd and APX activities at 100, 200 and 300 μM Cd of MuSI transgenic tobacco were up to two times higher than those of wild-type tobacco. MuSI gene is shown to enhance the activities of antioxidant enzymes resulting in higher tolerance to oxidative stress compared with the control plant.
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ACE-Inhibitory Properties of Proteolytic Hydrolysates from Giant Jellyfish Nemopilema nomurai
Yoon, Ho-Dong,Kim, Yeon-Kye,Lim, Chi-Won,Yeun, So-Mi,Lee, Moon-Hee,Moon, Ho-Sung,Yoon, Na-Young,Park, Hee-Yeon,Lee, Doo-Seog The Korean Society of Fisheries and Aquatic Scienc 2011 Fisheries and Aquatic Sciences Vol.14 No.3
This study aimed to determine the degree of hydrolysis and angiotensin-I-converting enzyme (ACE)-inhibitory activity of Giant Jellyfish Nemopilema nomurai (jellyfish) hydrolysates. The degree of hydrolysis using six proteolytic enzymes (Alcalase, Flavozyme, Neutrase, papain, Protamex, and trypsin) ranged from 13.1-36.8% and the inhibitory activities from 20.46-79.58%. Using papain hydrolysate, we newly isolated and characterized ACE-inhibitory peptides with a molecular weight of 3,000-5,000 Da that originated from jellyfish collagen. The purified peptide (FII-b) was predicted to be produced from an alpha-2 fragment of the type IV collagen of jellyfish. The N-terminal sequence of FII-b was Asp-Pro-Gly-Leu-Glu-Gly-Ala-His-Gly- and showed 87% identity to the collagen type IV alpha-2 fragment of Rattus norvegicus and a predicted protein from Nematostella vectensis, indicating that the ACE-inhibitory peptide originated from the collagen hydrolysate and had an $IC_{50}$ value of 3.8 ${\mu}g$/mL. The primary structure of the fragment is now being studied; this peptide represents an interesting new type of ACE inhibitor and will provide knowledge of the potential applications of jellyfish components as therapies for hypertension.
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