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RISS 인기검색어
- 검색키워드
- 저자 : Yongqing Cao (검색결과 4 건)
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Yongqing Cao,Tao Zeng,Wei Han,Xueying Ma,Tiantian Gu,Li Chen,Yong Tian,Wenwu Xu,Jianmei Yin,Guohui Li,Lizhi Lu,Shuangbao Gun Asian Australasian Association of Animal Productio 2024 Animal Bioscience Vol.37 No.1
Objective: Tibetan chickens, which have unique adaptations to extreme high-altitude environments, exhibit phenotypic and physiological characteristics that are distinct from those of lowland chickens. However, the mechanisms underlying hypoxic adaptation in the liver of chickens remain unknown. Methods: RNA-sequencing (RNA-Seq) technology was used to assess the differentially expressed genes (DEGs) involved in hypoxia adaptation in highland chickens (native Tibetan chicken [HT]) and lowland chickens (Langshan chicken [LS], Beijing You chicken [BJ], Qingyuan Partridge chicken [QY], and Chahua chicken [CH]). Results: A total of 352 co-DEGs were specifically screened between HT and four native lowland chicken breeds. Gene ontology and Kyoto encyclopedia of genes and genomes enrichment analyses indicated that these co-DEGs were widely involved in lipid metabolism processes, such as the peroxisome proliferator-activated receptors (PPAR) signaling pathway, fatty acid degradation, fatty acid metabolism and fatty acid biosynthesis. To further determine the relationship from the 352 co-DEGs, protein-protein interaction network was carried out and identified eight genes (ACSL1, CPT1A, ACOX1, PPARC1A, SCD, ACSBG2, ACACA, and FASN) as the potential regulating genes that are responsible for the altitude difference between the HT and other four lowland chicken breeds. Conclusion: This study provides novel insights into the molecular mechanisms regulating hypoxia adaptation via lipid metabolism in Tibetan chickens and other highland animals.
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Graphene Oxide Nanosheet-Composited Poly(N-isopropylacrylamide) Hydrogel for Cell Sheet Recovery
Yongqing Xia,Han Wu,Dachao Tang,Shuai Gao,Binghe Chen,Zhujun Zeng,Shengjie Wang,Meiwen Cao,Dongxiang Li 한국고분자학회 2019 Macromolecular Research Vol.27 No.7
Cell sheet engineering technique has been applied to treat various tissues without the use of a traditional scaffold. To date, methods for the cell sheet harvesting depend mostly on grafted poly(N-isopropylacrylamide) (pNIPAAm) thin layers, while the native pNIPAAm hydrogel, which possibly presents the easiest way to prepare thermo-responsive materials, is not suitable for the cell sheet harvesting due to its low cell attachment. In this study, the graphene oxide (GO) nanosheet was utilized as an additive to enhance the bio-compatibility of the pNIPAAm hydrogel. Different concentrations of GO nanosheets were added to prepare GO/pNIPAAm composite hydrogels through the in-situ free radical polymerization with polyethylene glycol dimethacrylate (PEGDA) as a cross-linker. The results indicated that the physical properties of the composite hydrogels had little difference with that of the native pNIPAAm hydrogel. However, the cell attachment, proliferation and detachment behaviors on the composite hydrogel surface were greatly enhanced. Monkey fibroblast COS7 cells attached and proliferated better on the GO/pNIPAAm composite hydrogel, while intact COS7 cell sheets could be harvested from the composite hydrogels by simply lowering the temperature. In contrast, the cells appeared as clusters on the native pNIPAAm hydrogel. Furthermore, when HeLa and COS7 cells were seeded successively onto the micropatterned GO/pNIPAAm hydrogel, there could be the formation of a patterned HeLa/COS7 cell layer. The geometrically patterned GO/pNIPAAm hydrogel may provide an easy-to-prepare material for releasing patterned cell sheets compared to the specific cell-adhesive proteins reported to make patterned cell layers.
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Responses to Starch Infusion on Milk Synthesis in Low Yield Lactating Dairy Cows
Yang Zou,Zhanshan Yang,Yongqing Guo,Shengli Li,Zhijun Cao 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.9
The effect of starch infusion on production, metabolic parameters and relative mRNA abundance was investigated in low yield lactating cows from 86 days in milk. Six Holstein cows fitted with permanent ruminal cannulas were arranged into one of two complete 3×3 Latin squares and infused with a starch solution containing 800 grams starch for 16 days. The three treatments were: i) ruminal and abomasal infusion with water (Control); ii) ruminal infusion with cornstarch solution and abomasal infusion with water (Rumen); iii) ruminal infusion with water and abomasal infusion with cornstarch solution (Abomasum). There were no significant differences (p>0.05) among the three treatments with low yield lactating cows in feed and energy intake, milk yield and composition, plasma metabolism, or even on gene expression. However, cows receiving starch through rumen performed better than directly through the abomasum during the glucose tolerance test procedure with a higher area under the curve (AUC; p = 0.08) and shorter half-time (t1/2; p = 0.11) of plasma insulin, therefore, it increased glucose disposal, which stated a lipid anabolism other than mobilization after energy supplementation. In conclusion, extra starch infusion at concentration of 800 g/d did not enhance energy supplies to the mammary gland and improve the lactating performance in low yield lactating cows.
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Responses to Starch Infusion on Milk Synthesis in Low Yield Lactating Dairy Cows
Zou, Yang,Yang, Zhanshan,Guo, Yongqing,Li, Shengli,Cao, Zhijun Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.9
The effect of starch infusion on production, metabolic parameters and relative mRNA abundance was investigated in low yield lactating cows from 86 days in milk. Six Holstein cows fitted with permanent ruminal cannulas were arranged into one of two complete $3{\times}3$ Latin squares and infused with a starch solution containing 800 grams starch for 16 days. The three treatments were: i) ruminal and abomasal infusion with water (Control); ii) ruminal infusion with cornstarch solution and abomasal infusion with water (Rumen); iii) ruminal infusion with water and abomasal infusion with cornstarch solution (Abomasum). There were no significant differences (p>0.05) among the three treatments with low yield lactating cows in feed and energy intake, milk yield and composition, plasma metabolism, or even on gene expression. However, cows receiving starch through rumen performed better than directly through the abomasum during the glucose tolerance test procedure with a higher area under the curve (AUC; p = 0.08) and shorter half-time ($t^{1/2}$; p = 0.11) of plasma insulin, therefore, it increased glucose disposal, which stated a lipid anabolism other than mobilization after energy supplementation. In conclusion, extra starch infusion at concentration of 800 g/d did not enhance energy supplies to the mammary gland and improve the lactating performance in low yield lactating cows.
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