Influence of water-stressed rice on feeding behavior of brown planthopper, Nilaparvata lugens (Stål)

Ye Tan,Mufei Zhu,Wenyan Xu,Wenwu Zhou,Dongdong Lu,Hanwu Shang,Zengrong Zhu 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2

Climate change can impact insects through abnormal weather conditions such as elevated temperatures and droughts. Crops experiencing periods of water stress from droughts may have significant effects on pest populations because of physiology changes, insect behavior and adaptation. Herewe report on the effects ofwater stress on the rice pest, the brown planthopper (BPH), Nilaparvata lugens (Stål). In themultiple choice test, both the fifth instar nymphs and female adults BPH preferred plants under the low stress level over control and high stress plants. The electrical penetration graph (EPG) showed that the fifth instar nymphs spent more time in non-penetration activities while the female adults spent less. Both the fifth instar nymphs and female adults spent more time in salivation on high stress plants than in control and low stress plants indicating difficulties in feeding. In addition there was marked increase in probe numbers made by both the fifth instar nymphs and the female adults in the feeding duration. The fifth instar nymphs made twice as many numbers of probes than those in the control (9.36 vs to 5.25 min) while the female adults made about 4 times more probes (13.13 vs 3.00 min). Both the fifth instar nymphs and the female adults producedmore honeydewon the lowstress plants suggesting that plants in low water stress favored insect feeding. These findings indicate that BPH in low water stress rice plants seem to do better which supports the theory that drought induces pest outbreaks.

Reliable Mobility Evaluation of Organic Field-Effect Transistors With Different Contact Metals

Huang, Fanming,Liu, Ao,Zhu, Huihui,Xu, Yong,Balestra, Francis,Ghibaudo, Gerard,Noh, Yong-Young,Chu, Junhao,Li, Wenwu IEEE 2019 IEEE electron device letters Vol.40 No.4

<P>The reliability of mobility evaluation of organic field-effect transistors (OFETs) is a serious issue because numerous overestimated and underestimated mobilities have been reported recently. A reliable approach to accurately evaluate the OFET mobility is therefore highly desirable. Here, in this letter, two commonly employed methods and the Y function method (YFM) were used to extract the mobilities of indacenodithiophene-co-benzothiadiazole (IDT-BT) OFETs with four different contact metals. The mobilities extracted by the commonly used methods varied greatly with the contact metal and channel length, whereas those extracted by the YFM were very stable and approached the intrinsic mobility of IDT-BT. Our results show that YFM is a precise approach that can be widely used to evaluate the OFET mobility.</P>

Effects of quercetin and coated sodium butyrate dietary supplementation in diquat-challenged pullets

Zhou Ning,Tian Yong,Liu Wenchao,Tu Bingjiang,Gu Tiantian,Xu Wenwu,Zou Kang,Lu Li Zhi 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.9

Objective: This study was designed to investigate the hypothesis that dietary quercetin (QUE) and coated sodium butyrate (SB) supplementation alleviate oxidative stress in the small intestine of diquat (DIQ)-challenged pullets. Methods: A total of 200 13-week-old pullets were divided into four groups: the control group (CON), the DIQ group, the QUE group, and the coated SB group, and injected intraperitoneally with either saline (CON) or diquat (DIQ, QUE, and SB) to induce oxidative stress on day 0. Results: On the first day, the malondialdehyde and superoxide dismutase (SOD) concentrations in the SB group were significantly different from those in the DIQ and QUE groups (p<0.05), and dietary supplementation with SB increased serum glutathione peroxidase (GSH-PX) levels compared with the DIQ group (p<0.05). Quercetin and SB increased the levels of CLAUDIN-1 and zonula occludens-1 (ZO-1) in the jejunum. On the tenth day of treatment, QUE attenuated the decrease in GSH-PX levels compared to those of the CON group (p<0.05), while SB increased SOD, GSH-PX, and total antioxidant capacity levels compared to those of the DIQ group. Nuclear factor erythroid 2-related factor 2 (NRF2) and heme oxygenase-1 (HO-1) mRNA levels in the QUE and SB groups increased (p<0.05) and CLAUDIN-1 mRNA levels in the QUE and SB groups were upregulated compared to those in the DIQ group ileum tissue. Conclusion: Supplementation of QUE and SB demonstrated the ability to relieve oxidative stress in pullets post DIQ-injection with a time-dependent manner and QUE and SB may be potential antioxidant additives for relieving oxidative stress and protecting the intestinal barrier of pullets. Objective: This study was designed to investigate the hypothesis that dietary quercetin (QUE) and coated sodium butyrate (SB) supplementation alleviate oxidative stress in the small intestine of diquat (DIQ)-challenged pullets.Methods: A total of 200 13-week-old pullets were divided into four groups: the control group (CON), the DIQ group, the QUE group, and the coated SB group, and injected intraperitoneally with either saline (CON) or diquat (DIQ, QUE, and SB) to induce oxidative stress on day 0.Results: On the first day, the malondialdehyde and superoxide dismutase (SOD) concentrations in the SB group were significantly different from those in the DIQ and QUE groups (p<0.05), and dietary supplementation with SB increased serum glutathione peroxidase (GSH-PX) levels compared with the DIQ group (p<0.05). Quercetin and SB increased the levels of CLAUDIN-1 and zonula occludens-1 (ZO-1) in the jejunum. On the tenth day of treatment, QUE attenuated the decrease in GSH-PX levels compared to those of the CON group (p<0.05), while SB increased SOD, GSH-PX, and total antioxidant capacity levels compared to those of the DIQ group. Nuclear factor erythroid 2-related factor 2 (NRF2) and heme oxygenase-1 (HO-1) mRNA levels in the QUE and SB groups increased (p<0.05) and CLAUDIN-1 mRNA levels in the QUE and SB groups were upregulated compared to those in the DIQ group ileum tissue.Conclusion: Supplementation of QUE and SB demonstrated the ability to relieve oxidative stress in pullets post DIQ-injection with a time-dependent manner and QUE and SB may be potential antioxidant additives for relieving oxidative stress and protecting the intestinal barrier of pullets.

Comparative analysis of liver transcriptome reveals adaptive responses to hypoxia environmental condition in Tibetan chicken

Yongqing Cao,Tao Zeng,Wei Han,Xueying Ma,Tiantian Gu,Li Chen,Yong Tian,Wenwu Xu,Jianmei Yin,Guohui Li,Lizhi Lu,Shuangbao Gun Asian Australasian Association of Animal Productio 2024 Animal Bioscience Vol.37 No.1

Objective: Tibetan chickens, which have unique adaptations to extreme high-altitude environments, exhibit phenotypic and physiological characteristics that are distinct from those of lowland chickens. However, the mechanisms underlying hypoxic adaptation in the liver of chickens remain unknown. Methods: RNA-sequencing (RNA-Seq) technology was used to assess the differentially expressed genes (DEGs) involved in hypoxia adaptation in highland chickens (native Tibetan chicken [HT]) and lowland chickens (Langshan chicken [LS], Beijing You chicken [BJ], Qingyuan Partridge chicken [QY], and Chahua chicken [CH]). Results: A total of 352 co-DEGs were specifically screened between HT and four native lowland chicken breeds. Gene ontology and Kyoto encyclopedia of genes and genomes enrichment analyses indicated that these co-DEGs were widely involved in lipid metabolism processes, such as the peroxisome proliferator-activated receptors (PPAR) signaling pathway, fatty acid degradation, fatty acid metabolism and fatty acid biosynthesis. To further determine the relationship from the 352 co-DEGs, protein-protein interaction network was carried out and identified eight genes (ACSL1, CPT1A, ACOX1, PPARC1A, SCD, ACSBG2, ACACA, and FASN) as the potential regulating genes that are responsible for the altitude difference between the HT and other four lowland chicken breeds. Conclusion: This study provides novel insights into the molecular mechanisms regulating hypoxia adaptation via lipid metabolism in Tibetan chickens and other highland animals.