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- 저자 : Yongkun Liu (검색결과 3 건)
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Dynamic changes of multi-notoginseng stem-leaf ginsenosides in reaction with ginsenosidase type-I
Yongkun Xiao,Chun-Ying Liu,임완택,Shuang Chen,Kangze Zuo,Hong Shan Yu,Jian-Guo Song,Long-Quan Xu,Tea-Hoo Yi,Feng Xie Jin 고려인삼학회 2019 Journal of Ginseng Research Vol.43 No.2
Background: Notoginseng stem-leaf (NGL) ginsenosides have not been well used. To improve their utilization, the biotransformation of NGL ginsenosides was studied using ginsenosidase type-I from Aspergillus niger g.848. Methods: NGL ginsenosides were reacted with a crude enzyme in the RAT-5D bioreactor, and the dynamic changes of multi-ginsenosides of NGL were recognized by HPLC. The reaction products were separated using a silica gel column and identified by HPLC and NMR. Results: All the NGL ginsenosides are protopanaxadiol-type ginsenosides; the main ginsenoside contents are 27.1% Rb3, 15.7% C-Mx1, 13.8% Rc, 11.1% Fc, 7.10% Fa, 6.44% C-Mc, 5.08% Rb2, and 4.31% Rb1. In the reaction of NGL ginsenosides with crude enzyme, the main reaction of Rb3 and C-Mx1 occurred through Rb3/C-Mx1/C-Mx; when reacted for 1 h, Rb3 decreased from 27.1% to 9.82 %, C-Mx1 increased from 15.5% to 32.3%, C-Mx was produced to 6.46%, finally into C-Mx and a small amount of C-K. When reacted for 1.5 h, all the Rb1, Rd, and Gyp17 were completely reacted, and the reaction intermediate F2 was produced to 8.25%, finally into C-K. The main reaction of Rc (13.8%) occurred through Rc/C-Mc1/CMc/ C-K. The enzyme barely hydrolyzed the terminal xyloside on 3-Oe or 20-O-sugar-moiety of the substrate; therefore, 9.43 g C-Mx, 6.85 g C-K, 4.50 g R7, and 4.71 g Fc (hardly separating from the substrate) were obtained from 50 g NGL ginsenosides by the crude enzyme reaction. Conclusion: Four monomer ginsenosides were successfully produced and separated from NGL ginsenosides by the enzyme reaction.
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Dynamic changes of multi-notoginseng stem-leaf ginsenosides in reaction with ginsenosidase type-I
Xiao, Yongkun,Liu, Chunying,Im, Wan-Teak,Chen, Shuang,Zuo, Kangze,Yu, Hongshan,Song, Jianguo,Xu, Longquan,Yi, Tea-Hoo,Jin, Fengxie The Korean Society of Ginseng 2019 Journal of Ginseng Research Vol.43 No.2
Background: Notoginseng stem-leaf (NGL) ginsenosides have not been well used. To improve their utilization, the biotransformation of NGL ginsenosides was studied using ginsenosidase type-I from Aspergillus niger g.848. Methods: NGL ginsenosides were reacted with a crude enzyme in the RAT-5D bioreactor, and the dynamic changes of multi-ginsenosides of NGL were recognized by HPLC. The reaction products were separated using a silica gel column and identified by HPLC and NMR. Results: All the NGL ginsenosides are protopanaxadiol-type ginsenosides; the main ginsenoside contents are 27.1% Rb3, 15.7% C-Mx1, 13.8% Rc, 11.1% Fc, 7.10% Fa, 6.44% C-Mc, 5.08% Rb2, and 4.31% Rb1. In the reaction of NGL ginsenosides with crude enzyme, the main reaction of Rb3 and C-Mx1 occurred through Rb3${\rightarrow}$C-Mx1${\rightarrow}$C-Mx; when reacted for 1 h, Rb3 decreased from 27.1% to 9.82 %, C-Mx1 increased from 15.5% to 32.3%, C-Mx was produced to 6.46%, finally into C-Mx and a small amount of C-K. When reacted for 1.5 h, all the Rb1, Rd, and Gyp17 were completely reacted, and the reaction intermediate F2 was produced to 8.25%, finally into C-K. The main reaction of Rc (13.8%) occurred through Rc${\rightarrow}$C-Mc1${\rightarrow}$C-Mc${\rightarrow}$C-K. The enzyme barely hydrolyzed the terminal xyloside on 3-O- or 20-O-sugar-moiety of the substrate; therefore, 9.43 g C-Mx, 6.85 g C-K, 4.50 g R7, and 4.71 g Fc (hardly separating from the substrate) were obtained from 50 g NGL ginsenosides by the crude enzyme reaction. Conclusion: Four monomer ginsenosides were successfully produced and separated from NGL ginsenosides by the enzyme reaction.
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Shiqing Sun,Yongkun Liu,Guohua Jiang,Bo Yu,Uwamahoro Evariste,Pianpian Ma 대한금속·재료학회 2019 ELECTRONIC MATERIALS LETTERS Vol.15 No.3
The fl exible electrodes are the essential components for fabricating modern fl exible supercapacitors. In this study, a compositehybrid fi lm integrated with silver nanoparticles (Ag NPs), reduced graphene oxide (rGO) and γ-MnO 2 nanorods asa fl exible electrode has been developed by the electrochemical deposition method. The substrate (rGO) of the electrode notonly provides mechanical stability but also ensures fast electron transfer during charge–discharge. The Ag NPs distributedin electrode provide the electronic channel to enhance the electric conductivity. The γ-MnO 2 electroplated on the surface ofhybrid fi lms further improved the electrochemical performance of them. The resultant γ-MnO 2 /Ag/rGO hybrid fi lms exhibita high specifi c capacitance of 1090.3 F g −1 at current density of 0.3 A g −1 . The asymmetric supercapacitor (ASC) deviceswere further fabricated by γ-MnO 2 /Ag/rGO fi lms as anode and AC/rGO fi lms as cathode electrodes within a wide operatingvoltage of 0–1.7 V. The energy density of obtained ASC devices was 71.8 Wh kg −1 at a power density of 279.4 W kg −1 .
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