韓國人의 HLA DQA1 遺傳座位에 대한 集團 遺傳學的 特性

남용석,김희선,이희석,이혜린,황적준 大韓法醫學會 1995 대한법의학회지 Vol.19 No.1

Using reverse dot blotting technique, genotype of HLA DQAl locus have been determined from 142 unrelated Korean individuals. Twenty genotypes were found from possible twenty one genotypes - the missing one was A2/A2 that had lowest expected frequency. All of known 6 alleles were found with each of its frequency being 15.1% for A1. 1, 16.6%for A1.2, 12.7% for A1.3, 11.6% for A2, 25.7% for A3 and 18.3% for A4. After X?-test(p>0.1), G-test(p>0.05), and by comparision of expected (0.82) and observed heterozygosity(0.81), the population was confirmed to be on the state of Hardy-Weinberg equilibrium. The gene diversity(0.82) of Korean population, which generally thought to be a group of single unity, actually was higher than that of most other populations. The pattern of alelic distribution was different from that of other populations, especially allele A1.3 which displayed heterogeneity between other goups with significance(p<0.01), as it turned out to have anthropological significance. After all, this HLA DQA1 system, even though its small number of alleles, having high degree of heterozygosity, was proven to be effective in individual identification, and paternity testing in Koean population.

pV47-2 다좌위탐식자를 이용한 인체 게놈에서 다형성 유전좌위의 분리

남용석,이혜린,한길로,황적준 大韓法醫學會 1997 대한법의학회지 Vol.21 No.2

Two polymorphic loci, so- called FS106 and FS185, have been isolated from the human genome, using a multilocus probe pV47-2, which is extensively used in Korea for forensic investigation such as resolving paternity disputes. Among the several plaques selected from λ Fix-II genomic libraries, fourteen clones have been characterized. Restriction maps of 14 clones were constructed to define the flanking as well as repeat parts. The repeat-free flanking DNA fragments were tested for single locus specific polymorphism, and repeat containing DNA fragments were sequenced for the design of PCR primers. None of the repeat-free flanking DNA fragments was not shown any polymorphisms by RFLP analysis. The (GGT)??-rich sequences in most of repeat containing DNA fragments were identified by sequencing analysis. Most of repetitive sequences consists of major units of (GGT)??, but a regular repetition pattern can not be found in all clones. Two sets of primers designed from flanking sequences of repeat containing DNA fragments were shown length polymorphisms by PCR analysis, when tested in 50 unrelated individuals. Three and four alleles were detected at FS106 and 0.58 for FS185. In addition, two loci, FS106 and FS185, have been mapped on chromosome 5 and 3, respectively, by somatic cell hybrid analysis.

미토콘드리아 DNA 염기서열 분석법에 의한 가족관계의 규명

남용석,이희석,김희선,이혜린,황적준 大韓法醫學會 1995 대한법의학회지 Vol.19 No.1

The human mitochondrial DNA has two characteristics that make it possible to identify individuals and establish family relationships. First, it is haploid, being exhibited only maternal inheritance. Second, it is highly variable on the hypervariable control region of mitochondrial DNA. Taking advantage of two characteristics of mitochondrial DNA, individual relationships in dispute were identified by combining PCR amplification with direct mitochondrial DNA sequencing. Two persons who alleged the same maternal lineage were identical on the mitochondrial DNA sequences from 15,960 to 16,569 and from 1 to 533. Other two persons were identical on the mitochondrial DNA sequences from 16221 to 16390 each other. However, seven bases are different on the sequences from 16221 to 16390 between two groups,. Even though four persons are kinship, these results suggest that they should come from two different maternal lineage.

삼풍백화점 붕괴사고 희생자들의 신원확인을 위한 유전자검사

남용석,이혜린,김경훈,김희선,이희석,황적준 大韓法醫學會 1996 대한법의학회지 Vol.20 No.1

A DNA typing was performed to identify decomposed body remains from Sampoong Department mass disaster in June 1995. These body parts include bone fragment, skin tissue, hairs, from which the extracted DNAs were highly degraded. Two VNTR loci, 4STR loci, and amelogenin gene were chosen for AMP-FLP, and mtDNA sequence analysis for the confirmation of maternal relationship. The results of AMP-FLP of the selected polymorphic loci showed different sucess rate for PCR. DIS80 and D17S5 loci were amplified successfully form 64.5%, and 67% of the samples, respectively. HUMTHOI, HUMCSF1PO, and HUMTPOX loci were amplified successfully from 90.3% of the samples each. HUMACTBP2 and amelogenin was amplified in 87% of the cases submitted. THE DNA types of 33 remains were compared with those of 81 bereaved families consisting of 173 member. Thirty three samples were reduced to 28 in numbers according to results of the same DNA types. Among them, the DNA types of 15 remains matched with those of bereaved families and the identified remains were reconfirmed by amelogenin sex typing and mitochondrial DNA sequence analysis. The others were not identified a family by failures of PCR amplification or non-matching of DNA types. Also it is confirmed that one hair sample should be artificial by non-digestion of protease and another be animal bone by result of dot blotting with human Alu probe. Our results indicate that multiplex PCR system consisting of several STR loci like HUMCSF1PO, HUMTPOX, and HUMTHO1 is more effective for the identification of highly decomposed human remains from mass disaster.

소아의 상완골 원위부 과간 골절

이우석,정환용,김우식,김용찬,전택수,김남현,김규태 대한골절학회 2004 대한골절학회지 Vol.17 No.4

상완골 원위부 과간 골절은 소아에서는 매우 드물게 발생되는 골절로 대부분 주관절의 굴곡상태로 주관절 후방부에 직접적인 충격에 의해 발생하는 것으로 보고되고 있다. 전위가 없거나 경미한 경우 도수적 정복술 혹은 도수적 정복술 후 경피적 핀고정술을 시행하며, 전위가 심한 골절이거나 도수적 정복술이 불가능한 경우 관혈적 정복술 후 핀고정술을 시행하는 것으로 알려져 있다. 저자들은 전위된 상완골 원위부 과간 골절 환아 2예에서 각각 도수적 정복술과 관혈적 정복술 후 K 강선 고정술을 시행하여 만족한 결과를 얻었기에 보고하는 바이다. Intercondylar fractures of the distal humerus are very rare in children. The pattern of the fracture would suggest that the mechanism of injury involves a fall directly on the flexed elbow. Most agree that the undisplaced fracture can be managed conservatively. With increasing displacement and comminution, the opinions tend to differ. We suggest that closed reduction with percutaneous K wire pinning and open reduction with percutaneous K wire pinning offer a satisfactory methods by which to treat displaced intercondylar fracture in children.

쇄골 골절에서 지연성 상완 신경총 마비 : 1예 보고 A Case Report

이우석,정환용,전택수,김용상,김남현 대한골절학회 2003 대한골절학회지 Vol.16 No.2

상완 신경총 마비는 쇄골 골절 후에 매우 드물게 발생하며, 대부분 많은 가골 형성을 동반한 쇄골의 중간1/3 부위 골절 불유합에서 발생한다. 골절의 치유 과정에서 과형성된 가골이 상완 신경총을 압박할 수 있으며, 감압술고 불유합에 대한 수술적 치료를 필요로 한다. 이에 저자들은 쇄골 골절 후 불유합과 과도한 가골 형성에 의해 발생된 지연성 상완 신경총 마비 환자를 치험 하였기에 보고하는 바이다. The brachial plexus palsies secondary to nonunion of the clavicle fracture are extremely rare. The nonunions are hypertrophic and usually in the middle third of the clavicle. Hypertrophic callus produced healing process will cause a compression of the neurovascular bundle. This lesion requires operative treatment for decompression of the brachial plexus and internal fixation of nonunion. We present a case of delayed brachial plexus palsy due to nonunion and excessive callus formation of a clavicular fracture.

蜂毒藥鍼刺戟이 腦幹 神經細胞와 Serotonin性 神經細胞의 活性變化에 미치는 영향

金惠南,高炯均,朴東錫,姜成吉,金容奭,崔容泰 경희대학교 동서의학연구소 2001 東西醫學硏究所 論文集 Vol.2000 No.-

This study was designed to evaluate the effect of the bee venom(BV) aqua-acupuncture on the neuronal activities of serotonergic(5-HT) system in the brainstem. After the BV aqua-acupuncture was applied on Chok-Samni(ST36) and the gluteal part(Blank locus) in rats, the number of Fos immunoreactive neurons was counted by using computerized image analyzing system. Also, the number of colocalization between 5-HT containing neurons Fos immunoreactive neurons were analyzed by using the double immunohistochemical technique. The results of the experiments were summarized as follows : 1.In almost every neucli, the Chok-Samni group and Blank locus group showed more increase in the number of Fos immunoreactive neurons than the control group. Especially, in Arc, DR, LC, RMg, Gi, PAG Rost and PAG LV, the Chok-Samni group showed more significant increase than the control group. Also, in PAG LV Mid and Arc, Chock-Samni group showed more significant increase than the Blank locus group. 2.In DR and PAG LV Mid, Chok-Samni group and the Blank locus group showed more significant increase in the number of colocalization between 5-HT containing neurons and Fos immunoreactive neurons than the control group after the BV aqua- acupuncture. Also, the Chok-Samni group showed more significant increase than the Blank locus group. Consequently, the BV aqua-aqupuncture increased more potent the number of Fos immunoreactive neurons and the activity of serotonergic neurons. Furthermore, the BV aqua-acupuncture was more effective on Chok-Samni than Blank locus group. These results indicate that the BV aqua-acupuncture is very effective therapy to control pain. The therapeutic effect of BV aqua-acupunture may associated with the endogenous modulatory system such as serotonin Those data from the study can be applied to establish the effective treatment of the BV for pain control in the clinical field.

PMDA-ODA 폴리이미드의 합성 및 특성에 관한 연구

정용재,한태성,최성부,남석태,김병식 동국대학교 산업기술연구원 1996 산업기술논문집 Vol.8 No.-

Pyromellitic dianhydride(PMDA) 와 Oxydianiline(ODA)를 N, N-dimethylacetamide (DMAc)에 녹여 Polyamic acid(PAA)를 2단계 열이미드화법으로 제조하였다. 제조된 폴리이미드 막의 이미드화도는 FT-IR에 의하여 분석되었다. 또한 PAA의 농도를 10~30wt%로 변화시키며 합성하여 열이미드화 시킨 후 농도에 따른 단면의 상태를 SEM사진을 통하여 관찰하였다. 농도 변화에 따른 밀도와 T_g의 변화는 혼합액체부상법과 DSC로 분석되었다. 열처리 온도를 증가시킬수록 이미드화도는 증가되었고 PAA의 농도가 증가할수록 필름의 단면이 치밀해짐을 알 수 있었다. 또한 제조된 PMDA-ODA 폴리이미드의 T_g는 215℃ 이었다. Polyamic acid(PAA) was synthesised with Pyromellitic dianhydride(PMDA) and Oxydianiline (ODA) in N, N-dimethylacetamide(DMAc). PMDA-ODA polyimide membranes were obtained by two step thermal imidization, and the degree of imidization of polyimide membranes was observed by the FT-IR. After the thermal imidization through the concentration of varied synthesis of PAA, 10~30wt%, SEM photographies of section state were observed. The density and glass transition temperature on the varied concentration were replace with investigated test of density and Differential Scanning Calorimetry (DSC). With increasing thermal treatment temperature, the degree of imidization was increased. While the concentration of PAA increases, the section of membrane was densed. The glass transition temperature was maintained to be constant(215℃) within the PAA concentration range.

당뇨병 유발쥐혈청내 methylated amino acid의 함량 변화

김용기,유세근,남석우,이향우 성균관대학교 약학연구소 1993 成均藥硏論文集 Vol.5 No.1

In order to investigate the relationship between methylated amino acids and diabetes, we investigated the changes of serum concentration of methylated amino acids in serum of the experimentally induced diabetic rats. The diabetic symptom was induced by injectly alloxan 100 mg/kg intraperitoneally. The serum content of N^G-monomethylarginine(MMA), N^G, N^G-dimethyl-arginine(DMA) and N^G, N^G-dimethylarginine(D'MA) were analyzed by HPLC. The results are as followings ① There was no change in serum content of MMA in the diabetic group. ② But it was observed that the amounts of DMA and D'MA were increased remarkably in the diabetic group. Specifically, D'MA content was increased about 7 times in comparision to that of the control group. ③ However, the concentration of trimethyllysine in RBC was decreased slightly in diabetic group but not significant statistically.

약초 추출액에 의한 향특소포자충 효과 및 대식세포의 면역활성

양용석,김인식,강성구,박주연,성호중,남호우,하지용 연세대학교 보건과학연구소 2001 보건과학논집 Vol.11 No.-