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        Transcriptional profiling reveals the molecular bases of immune regulation in Apis mellifera in response to Ascosphaera apis infection

        Bo Xu,Yingzhe WANG,Shixin ZHU,Haizhu ZHOU,Changlong GOU,Wenlong DONG,Yu Wang,Yunhang GAO,Hongxia MA 한국곤충학회 2019 Entomological Research Vol.49 No.1

        Chalkbrood, which results from Ascosphaera apis infection, is one of the bee diseases that causes serious damage to the bee colony. Understanding the molecular bases underlying immune response to chalkbrood disease would facilitate the genetic breeding of bees by selecting races with superior chalkbrood resistance. In this study, transcriptome sequencing was performed to identify genes and pathways involved in the immune response to As. apis infection in A. mellifera larvae. In total, 2,890 differentially expressed genes (DEGs) (FDR < 0.001) were identified between the healthy and As. apis infected bee larvae, including 2,214 up‐regulated and 676 down‐regulated unigenes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway classification of the DEGs revealed association with development, energy metabolism, signal transduction, disease, and immune response. Among the immune‐related genes, p38, toll‐like receptors (TLRs), integrin, and antimicrobial peptides were up‐regulated under As. apis infection. This study provides valuable gene transcriptional information towards the investigation of molecular mechanisms related to chalkbrood immune response and host pathogenesis in A. mellifera.

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        Efficient extraction and separation of vanadium and chromium in high chromium vanadium slag by sodium salt roasting-(NH4)2SO4 leaching

        Jing Wen,Tao Jiang,Yingzhe Xu,Jing Cao,Xiangxin Xue 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.71 No.-

        A novelty process based on sodium salt roasting-(NH4)2SO4 leaching was proposed to extract vanadiumand chromium in high chromium vanadium slag (HCVS). V2O5 and Cr2O3 was then prepared. The effectsof roasting and leaching conditions on vanadium and chromium extraction behavior were studiedsystematically and completely. Vanadium precipitation conditions and chromium reduction conditionswere optimized further. 94.6% vanadium and 96.5% chromium were extracted when HCVS and Na2CO3were mixed in the molar ratio of n(Na2CO3)/n(V2O3 + Cr2O3) of 2.5, then leached in 30 g/L (NH4)2SO4solution. 94.8% vanadium was precipitated as ammonium polyvanadate (APV) just by adjusting theleaching liquid pH at 4.5, almost all chromium was remained in liquid, achieving the efficient separationof vanadium and chromium. Chromium was then recovered by reduction and precipitation. More than99% chromium was reduced when Na2S2O5 was added in m(Na2S2O5)/m[Cr(VI)] above 3. By roasting thedeposits of vanadium and chromium respectively, 91.49% V2O5 and 89.89% Cr2O3 were obtained. Thesupernatant after vanadium and chromium extraction containing NH4+ could be recycled as the newleaching medium with some new (NH4)2SO4 added, which greatly reduced the discharge of ammonianitrogenwastewater and made the whole process more environmentally friendly.

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