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Zheng, Shuzhen,Friehs, Karl,He, Ning,Deng, Xu,Li, Qingbiao,He, Zheng,Xu, Chenyun,Lu, Yinghua Korean Society for Biotechnology and Bioengineerin 2007 Biotechnology and Bioprocess Engineering Vol.12 No.3
In this study we investigated the effects of carbon and nitrogen sources, and the carbon to nitrogen ratio (C:N) of growth medium on the production of plasmid DNA by $pUK21CMV{\beta}1.2$ harbouring recombinant E. coli. The results indicated that sucrose and casein peptone were the most suitable carbon and nitrogen sources, respectively, for plasmid production, and the plasmid concentration was significantly influenced by the C:N ratio. A Box-Behnken design and surface response methodology were employed to formulate the optimal medium. In the optimized medium, a plasmid concentration of 51.80mg/L and a plasmid selectivity of 23.49mg/g were achieved after 24h of incubation. These values were approximately 3.5 and 1.7 times higher, respectively, than those obtained on the original LB medium.
Optimization of Medium Components for Plasmid Production by Recombinant E. coli DH5α pUK21CMVβ1.2
Yinghua Lu,Shuzhen Zheng,Karl Friehs,Ning He,Xu Deng,Qingbiao Li,Zheng He,Chenyun Xu 한국생물공학회 2007 Biotechnology and Bioprocess Engineering Vol.12 No.3
In this study we investigated the effects of carbon and nitrogen sources, and the carbon to nitrogen ratio (C:N) of growth medium on the production of plasmid DNA by pUK21CMVβ1.2 harbouring recombinant E. coli. The results indicated that sucrose and casein peptone were the most suitable carbon and nitrogen sources, respectively, for plasmid production, and the plasmid concentration was significantly influenced by the C:N ratio. A Box-Behnken design and surface response methodology were employed to formulate the optimal medium. In the optimized medium, a plasmid concentration of 51.80 mg/L and a plasmid selectivity of 23.49 mg/g were achieved after 24 h of incubation. These values were approximately 3.5 and 1.7 times higher, respectively, than those obtained on the original LB medium.
Distribution Rules of Nutrients in Orobanche Aegyptiaca-Tomato Parasitic System
Jinhu Zhi,Jinfeng Chen,Qiangqing Zheng,Shuzhen Zhao,Desheng Wang 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.3
The research put forward the concept of parasitic system for the first time and studied distribution rules of N, P and K nutrients of different soil areas and different plant tissues in the system along with changes in the tomato growth period, as well as relevance between rhizosphere soil and nutrient contents in different plant tissues. It is found in the results that when Orobanche aegyptiaca was parasitized in tomatoes and joined nutrient transmission via a certain channel, the nutrients in tomato rhizosphere soil would firstly be transported into aboveground parts and Orobanche aegyptiaca by its transfusion tissue; content changes of rapidly-available N, P and K in Orobanche aegyptiaca rhizosphere soil, tomato rhizosphere soil and non-rhizosphere soil showed certain high-low complementarity; content changing trends of N, P and K in different organs of tomatoes and Orobanche aegyptiaca had certain relations, but would differ along with variation of tomato growth period; contents of rapidly-available N and K in Orobanche aegyptiaca rhizosphere soil showed significant correlation with total contents of N and K in organs of tomato and Orobanche aegyptiaca.