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Dark Field Digital Holographic Microscopy Based on Two-lens 360-degree Oblique Illumination
Xiuying Zhang,Yingchun Zhao,Caojin Yuan,Shaotong Feng,Lin Wang 한국광학회 2020 Current Optics and Photonics Vol.4 No.3
In this paper we propose a dark-field digital holographic microscopy system based on 360-degree oblique illumination. This setup is constructed without using a dark-field objective. The principle of 360-degree oblique illumination of vortex beam and dark-field digital holographic microscopy are introduced theoretically and experimentally. By analyzing the reconstructed image of a dark-field digital hologram of a USAF 1951 target, it is proved that the imaging resolution can be improved by this method. And also, comparison and analysis are made on the reconstructed image of a bright-dark field digital hologram of a pumpkin stem slice, the result shows that the imaging contrast is also enhanced with this method, and it is effective for dark-field digital holographic microscopy imaging of large transparent biological samples.