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Comparative portrayal of ocular surface microbe with and without dry eye
ZhenHao Li,Yufang Gong,ShuZe Chen,SiQi Li,Yu Zhang,HuiMin Zhong,ZhouCheng Wang,YiFan Chen,QiXin Deng,YuTing Jiang,LiYing Li,Min Fu,GuoGuo Yi 한국미생물학회 2019 The journal of microbiology Vol.57 No.11
To compare the ocular surface (OS) microbial communities and diversity between dry eye (DE) and non-DE (NDE). Furthermore, we compared meibomian gland dysfunction (MGD) and non-MGD (NMGD) among DE subjects. The V3-V4 region of 16S rRNA gene high-throughput sequencing was performed in the conjunctival swab samples to investigate the composition of the OS bacterial community in DE (n=35) and NDE (n=54) and compared the composition of MGD (n=25) and NMGD (n=10) among DE subjects. Deep sequencing of OS 16S rDNA from DE (n=35) and NDE (n=54) demonstrated great a difference in alpha and beta diversity between the OS bacterial flora (P < 0.05). The similar OS microbial structures were shown at the phylum and genus levels by bioinformatics analysis between them, and in LEfSe (linear discriminant analysis effect size) analysis, Bacteroidia and Bacteroidetes were enriched in DE, while Pseudomonas was plentiful in NDE (linear discriminant analysis [LDA] > 4.0). Among the DE group, there was no significant difference in α and β diversity between MGD and NMGD (P > 0.05). Surprisingly, Bacilli was the dominant microbe in MGD, and Bacteroidetes was the superior bacteria in NMGD among DE subjects (LDA > 4.0). Different diversity of OS bacteria composition between DE and NDE and the altered diversity of OS bacteria may play an important role in DE. Moreover, the lower dominance of OS bacteria in DE may be associated with the occurrence and development of DE. Although there was no significant difference in alpha and beta analysis, the OS dominant microbe between MGD and NMGD among DE was different.
Cyclic response of self-centering SRC walls with frame beams as boundary
Lu Sui,Hanheng Wu,Penghui Tang,Tao Gong,Yifan Wang 국제구조공학회 2021 Steel and Composite Structures, An International J Vol.40 No.1
In recent years, earthquake resilient structures have become a research focus in the field of seismic engineering. In order to improve the resilience of steel frame - concrete wall panel structures, an innovative self-centering SRC wall panels with replaceable energy dissipaters is presented in the paper. For this purpose, the tested subassembly involving the self-centering SRC walls with frame beams as boundary was presented. The wall panels used in steel moment frames can provide the lateral resistance for structures. Test models of the wall panels with partial framed beams as boundary were suggested. The full-scale specimens were tested under cyclic lateral loads. The configuration of energy dissipaters and initial force of PT steel bars were varied to study their impacts on lateral behavior of the walls. Furthermore, the numerical models based on ABAQUS were developed. The parameter studies, including diameter of PT tendons, beam profile, beam span and wall width, were then conducted. The PT tendons can provide self-centering ability and the dampers are used to dissipate energy. The failure of the wall panels is mainly concentrated on energy dissipaters. This case makes it possible to repair the walls easily.
A Novel Two-way Time Synchronization Protocol for Fusion Application
Yong Pang,Guangming Li,Xiaoming Wu,Fuqiang Wang,Yifan Hu,Qingchao Gong 보안공학연구지원센터 2016 International Journal of Future Generation Communi Vol.9 No.8
Data fusion application is one of the most popular applications in Wireless sensor networks (WSNs). Time synchronization is an important technology for data fusion applications due to its necessary for data fusion in the networks. Aimed at reliable time synchronization performance, this paper proposes a novel two-way time synchronization protocol (NTSP). Combing node’s clock model and two-way packet exchange mechanism, NTSP makes time synchronization protocol more precise and much steadier. NTSP adopts simulated annealing (SA) algorithm to defend random noise and malicious delay attacks. Network simulations are carried out to analyze performances of NTSP on time synchronization accuracy, convergent rate and defensive degree for malicious delay attacks. Comparing to traditional time synchronization protocols, NTSP is an effective time synchronization protocol in WSNs.
( Lili Gu ),( Chao Ding ),( Hongliang Tian ),( Bo Yang ),( Xuelei Zhang ),( Yue Hua ),( Yifan Zhu ),( Jianfeng Gong ),( Weiming Zhu ),( Jieshou Li ),( Ning Li ) 대한소화기기능성질환·운동학회(구 대한소화관운동학회) 2017 Journal of Neurogastroenterology and Motility (JNM Vol.23 No.2
Background/Aims Chronic intestinal pseudo-obstruction (CIPO) is a serious, life-threatening motility disorder that is often related to bacterial overgrowth. Fecal microbiota transplantation (FMT) results in restoration of the normal intestinal microbial community structure. We investigated the efficacy of FMT in the treatment of CIPO patients. Methods Nine patients (age 18-53 years) with CIPO were enrolled in this prospective, open-label study. Patients received FMT for 6 consecutive days through nasojejunal (NJ) tubes and were followed up for 8 weeks after treatment. We evaluated the rate of clinical improvement and remission, feeding tolerance of enteral nutrition, and CT imaging scores of intestinal obstructions. Lactulose hydrogen breath tests were performed before FMT and 8 weeks after FMT to evaluate for the presence small intestinal bacterial overgrowth (SIBO). Results FMT significantly alleviated bloating symptoms, and symptoms of pain were relieved 2 weeks after FMT. Enteral nutrition administered through a NJ tube after FMT was well-tolerated by 66.7% (6/9) of patients. CT scores of intestinal obstructions were significantly reduced after FMT (P = 0.014). SIBO was eliminated in 71.0% (5/7) of patients. Conclusions This pilot study demonstrated the safety of using FMT. FMT may relieve symptoms in selected patients with CIPO. FMT may also improve patient tolerance of enteral nutrition delivered via a NJ tube. (J Neurogastroenterol Motil 2017;23:289-297)