Mechanical Stretch Promotes Invasion of Lung Cancer Cells via Activation of Tumor Necrosis Factor-alpha

Liang-Kun Chen,Ching-Chi Hsieh,Yi-Chao Huang,Yuan-Jung Huang,Chun-Fan Lung,Wei-En Hsu,Chao-Ling Yao,Tsung-Yu Tseng,Chi-Chung Wang,Yi-Chiung Hsu 한국생물공학회 2023 Biotechnology and Bioprocess Engineering Vol.28 No.3

Most of the gas exchange in the human body is carried out by the lungs, and the physiological activities of the lungs are uninterrupted. Due to the deterioration of the external environment, pulmonary cell lesions are common clinical lung diseases. Mechanical cyclic stretching is one kind of bionic technology to observe lung cancer cells. The A549 cell line is the human lung adenocarcinoma cell line derived from a primary lung tumor. This study investigated the effects of mechanical cyclic stretching on A549 cell activity and gene expression profile. Whereas mechanical cyclic stretching had no significant difference in colony formation and cell migration of A549 cells, the cell invasion increased significantly in A549 cells after stretching. In addition, the microarray data showed that mechanical cyclic stretching altered gene expression, induced inflammation of cells, and activation of Wnt/β- catenin and tumor necrosis factor pathways. More importantly, mechanical cyclic stretching activated the expression of tumor necrosis factor-alpha (TNF-α) protein. Therefore, the increase of cell invasion induced by mechanical cyclic stretching might be associated with the activation of TNF-α in human lung adenocarcinoma cells.

Real-World Effectiveness and Safety of SOF/LDV for Pa-tients with Chronic Hepatitis C in Taiwan

( Ching-chu Lo ),( Pin-nan Cheng ),( Chi-yi Chen ),( Chung-feng Huang ),( Hsing-tao Kuo ),( Kuo-chih Tseng ),( Yi-hsiang Huang ),( Chi-ming Tai ),( Cheng-yuan Peng ),( Ming-jong Bair ),( Chien-hung Ch 대한간학회 2021 춘·추계 학술대회 (KASL) Vol.2021 No.1

Comparisons of Moisturizing Function Between Rayon Fabric with Collagen Peptides from Fish Scales and Regular Rayon Fabric Under Various Relative Humidity

Chi‑Shih Huang,Erh‑Jen Hou,Ying‑Chou Lee,Tzong‑Huei Lee,Yi‑Jun Pan,Ta Yu,Wei‑Hsin Lin,Chun‑Han Shih,Wei‑Che Chang 한국섬유공학회 2023 Fibers and polymers Vol.24 No.12

The study was inspired by the specialized facial masks made of rayon non-woven fabrics which contained collagen peptides for improving moisturizing function. This study explored the moisturizing function of a rayon fabric containing collagen peptides extracted from tilapia fish scales under various conditions of relative humidity. This research had implications for the development of clothing that can prevent dry skin. A two-stage nested design experiment was adopted. The first-stage factor such as the fabric has two levels and the second-stage factor such as the relative humidity with three levels nested under each level of the first-stage factor. Preliminary results indicated that introducing a new variable (i.e., fabric moisturizing value, which combines the moisture regains of adsorption and desorption) would be useful. The moisturizing value of the novel rayon fabric and regular rayon fabric increased with the increase in relative humidity, and moisturizing effect of the novel rayon fabric with collagen peptides was better than that of the regular rayon fabric. Therefore, the novel rayon fabric may be suitable for preventing dry skin in winter.

Risks of cervical intraepithelial neoplasia grade 3 or invasive cancers in ASCUS women with different management: a population-based cohort study

Yi-Jou Tai,Yun-Yuan Chen,Huang-Cheng Hsu,Chun-Ju Chiang,San-Lin You,Chi-An Chen,Wen-Fang Cheng,Taiwan Cervical Cancer Control Task Force 대한부인종양학회 2018 Journal of Gynecologic Oncology Vol.29 No.4

OBJECTIVE: To investigate the progression risk of atypical squamous cells of undetermined significance (ASCUS) with different clinical managements. METHODS: Women with their first diagnosis of ASCUS cytology were retrieved from the national cervical cancer screening database and linked to the national health insurance research database to identify the management of these women. The incidences of developing cervical intraepithelial neoplasia grade 3 and invasive cervical cancer (CIN3+) were calculated, and the hazard ratios (HRs) were estimated using a Cox proportional hazards model. This study was approved by the Research Ethics Committee of the National Taiwan University Hospital and is registered at ClinicalTrials.gov (Identifier: NCT02063152). RESULTS: There were total 69,741 women included. Various management strategies including colposcopy, cervical biopsies and/or endocervical curettage, and cryotherapy, failed to reduce the risk of subsequent CIN3+ compared with repeat cervical smears. Loop electrosurgical excision procedure/conization significantly decreased risk of subsequent CIN3+ lesions (HR=0.22; 95% confidence interval [CI]=0.07–0.68; p=0.010). Women in their 40s–50s had an approximately 30% risk reduction compared to other age groups. Women with a previous screening history >5 years from the present ASCUS diagnosis were at increased risk for CIN3+ (HR=1.24; 95% CI=1.03–1.49; p=0.020). CONCLUSION: In women of first-time ASCUS cytology, a program of repeat cytology can be an acceptable clinical option in low-resource settings. Caution should be taken especially in women with remote cervical screening history more than 5 years.

새로운 다수제적 혼합제도에 대한 대만 시민의 인식도 -코호트 분석

( Chi Huang ),( Ching Hsin Yu ),( Yi Ching Hsiao ) 한국선거학회 2011 선거연구 Vol.1 No.2

선거제도는 투표를 의석으로 전환하는 규칙을 제공하며, 그에 따라 선거에서 누가 승리하고 패배하는가를 결정한다. 선거제도가 정치에서 수행하는 역할에 의문을 던지는 사람은 별로 없을 것이다. 그러나 기존 연구는 일반 시민들이 선거제도를 어떻게 인식하고 이해하고 있는가 (특히 선거제도가 근본적 변화를 경험하고 있는 이행기 동안에) 라는 질문에 대해서 이상하게도 침묵을 지키고 있다. 대만의 선거제도 개혁 경험은 이러한 빈 공간을 채울 수 있는 좋은 기회를 제공해 준다. 대만은 2008년에 의원 선거제도를 변화시켰는데, 지난 50년간 지속되어 왔던 단기비이양식(SNTV) 제도에서 다수제적 혼합선거제도 (MMM)로 바꿨다. 새로운 다수제적 혼합선거제도는 전체 의석 수, 선거구 크기, 선거 공식, 그리고 투표 구조 등에 있어서 과거의 단기비이양식 제도와 상당히 달랐다. 이 글은 새로운 선거제도를 소개하고 이 제도전반에 대한 시민들의 인식도를 알아보고자 한다. 또한 이 글에서는 시민들의 선거제도에 대한 지식이 2008년 선거 이전과 이후에 어떻게 변화했는지 살펴본다. 두 선거 제도 사이에 상당한 차이가 존재하기 때문에, 먼저 새로운 제도에 대한 시민들의 전반적 지식을 조사한다. 그 다음 설문조사 자료를 사용하여, 선거 기간과 비선거 기간 사이에 시민들의 지식 변화를 고찰한다. 분석 결과에 따르면, 다수의 시민들이 새로운 다수제적 혼합선거제도의 주요 구성 요인에 대해 완전히 이해하지 못하고 있다. 특히, 최소 조건에 대한 조항이 가장 적은 관심을 받고 있다. 더 나아가, 새로운 선거제도에 대한 시민들의 지식은 선거 모멘텀에 영향을 받아, 선거일이 다가옴에 따라 지식이 올라가고 선거일 이후에는 감소하는 것으로 나타났다. 마지막으로, 보다 구체적인 코호트 분석을 통해 시민들 중에 어떤 집단이 선거제도 변화와 관련된 정보를 가장 잘 획득하는지를 고찰한다. 본 연구 결과는 선거제도 지식에 대한 선거 주기 가설을 부분적으로 지지해준다. Electoral systems provide the rules of translating votes into seats and thus decide who wins and loses in elections. Few would doubt the critical roles that electoral systems play in politics. Yet current literature is curiously silent about how ordinary citizens perceive and understand the electoral system, especially during the transition period when the system is undergoing fundamental changes. The experiences of electoral reform in Taiwan provide an opportune case to fill up this gap. Taiwan transformed its legislative electoral system from a half-century long SNTV (single non-transferable vote) system to a MMM (mixed-member majoritarian) electoral system in 2008. The new MMM system is a sharp departure from the original SNTV system in the total number of legislative seats, the district magnitude, electoral formula, and ballot structure. This essay intends to introduce the new MMM system and explore citizens` awareness of the new MMM system in general. Also, it explores the changes of citizens` knowledge of the new electoral system before and after the 2008 legislative election. Given the significant differences between the SNTV system and the MMM system, it firstly tests of citizens` overall knowledge of the new MMM system. Then, with the help of survey data, it examines the changes of citizens` knowledge in election periods and non-election periods. The findings show a majority of citizens are not fully aware of the institutional components of the new MMM system. In particular, the threshold regulation receives the least attention by citizens. Furthermore, it finds that citizens` knowledge of the new MMM system is a function of electoral momentum, i.e., citizens` knowledge rises as the election date approaches and declines after the election date. Then we proceed with a more detailed cohort analyses to examine which groups of citizens are more likely to pick up information related to the electoral system changes. Our findings partially support our electoral cycle hypothesis of electoral system knowledge.

Development and characterization of a potential diagnostic monoclonal antibody against capsid protein VP1 of the chicken anemia virus

Yi-Yang Lien,Chi-Hung Huang,Fang-Chun Sun,Shyang-Chwen Sheu,Tsung-Chi Lu,Meng-Shiunn Lee,Shu-Chin Hsueh,Hsi-Jien Chen,Meng-Shiou Lee 대한수의학회 2012 JOURNAL OF VETERINARY SCIENCE Vol.13 No.1

Chicken anemia virus (CAV) is an important viral pathogen that causes anemia and severe immunodeficiency syndrome in chickens worldwide. In this study, a potential diagnostic monoclonal antibody against the CAV VP1 protein was developed which can precisely recognize the CAV antigen for diagnostic and virus recovery purposes. The VP1 gene of CAV encoding the N-terminus-deleted VP1 protein, VP1Nd129, was cloned into an Escherichia (E.) coli expression vector. After isopropyl-b-D-thiogalactopyronoside induction, VP1Nd129 protein was shown to be successfully expressed in the E. coli. By performing an enzyme-linked immunoabsorbent assay using two coating antigens, purified VP1Nd129 and CAV-infected liver tissue lysate, E3 monoclonal antibody (mAb) was found to have higher reactivity against VP1 protein than the other positive clones according to the result of limiting dilution method from 64 clones. Using immunohistochemistry, the presence of the VP1-specific mAb, E3, was confirmed using CAV- infected liver and thymus tissues as positive-infected samples. Additionally, CAV particle purification was also performed using an immunoaffinity column containing E3 mAb. The monoclonal E3 mAb developed in this study will not only be very useful for detecting CAV infection and performing histopathology studies of infected chickens, but may also be used to purify CAV particles in the future.

A Reconfigurable Software Distribution Framework for Smart Living Environments

Ing-Yi Chen,Chao-Chi Huang 보안공학연구지원센터 2007 International Journal of Smart Home Vol.1 No.2

This paper seeks to define a process that allows for the reconfiguration of management processes, in a manner that is easier than those afforded by existing approaches. This project proposes to achieve this through the use of a Software Distribution Management System (SDMS) that employs a service-oriented mechanism. This constitutes an improvement over more traditional distribution systems because SDMS allows for the reconfiguration of management distribution processes. In addition, this study applies a Service-Oriented Software Engineering (SOSE) development methodology for designing and building of a Service Oriented Architecture (SOA) based on SDMS. While based on a conventional methodology this gives consideration to added dimensions such as service choreography, and service bus. The result is a approach that allows software engineers to readily reconfigure and recombine services from a range of different systems, applications, architectures and ages. This paper provides the service identification results and monitoring methodology of the SDMS produced by this project.

Weekly topotecan chemotherapy in the treatment of recurrent ovarian cancer patients

( Wan Yi Huang ),( Wen Fang Cheng ),( Chang Yao Hsieh ),( Chi An Chen ) 대한산부인과학회 2007 대한산부인과학회 학술대회 Vol.93 No.-

A Simple Partial Discharge Detector for Low-Voltage Rotating Electrical Machines

Cheng-Chi Tai,Ting-Cheng Huang,Ching-Chau Su,Chien-Yi Chen,Ju-Chu Hsieh,Yu-Shiun Lin,Chung-Tzong Wang,Jeng-Hung Lai 전력전자학회 2007 ICPE(ISPE)논문집 Vol.- No.-

A new, simple partial discharge (PD) detector for low-voltage rotating electrical machines using acoustical emission (AE) technique is dseveloped in this study. Common electric components were used in the detector, which reduces the cost of inspection, comparing with the traditional PD detection methods that use expensive equipment costing from tens of thousands to several millions dollars. Experimental results by resonant type AE sensors (150 ㎑) which utilize power line-cycle in a microcontroller unit (MCU) as reference to measure the PDs generated in a low-voltage motor are presented. The AE signals are then amplified by a pre-amplifier (30 ㎑ ~ 300 ㎑, 34 ㏈). Since the resonant frequency of the sensor is much lower than that of the electromagnetic (EM) interferences around the motor, the effects of noise is substantially reduced by this method. In the mean time, the use of 150-㎑ resonant type AE sensor also avoids the disturbance of mechanical vibration noise. According to the experiment results, the measurement system developed in this study can be used to detect the PDs’ AE signals correctly. The AE measurement scheme proposed in this study provides an effective, low-cost method for PD measurements.

On the direct insulator-quantum Hall transition in two-dimensional electron systems in the vicinity of nanoscaled scatterers

Liang, Chi-Te,Lin, Li-Hung,Kuang Yoa, Chen,Lo, Shun-Tsung,Wang, Yi-Ting,Lou, Dong-Sheng,Kim, Gil-Ho,Yuan-Huei, Chang,Ochiai, Yuichi,Aoki, Nobuyuki,Chen, Jeng-Chung,Lin, Yiping,Chun-Feng, Huang,Lin, Sh Springer 2011 Nanoscale research letters Vol.6 No.1

<P>A direct insulator-quantum Hall (I-QH) transition corresponds to a crossover/transition from the insulating regime to a high Landau level filling factor ν > 2 QH state. Such a transition has been attracting a great deal of both experimental and theoretical interests. In this study, we present three different two-dimensional electron systems (2DESs) which are in the vicinity of nanoscaled scatterers. All these three devices exhibit a direct I-QH transition, and the transport properties under different nanaoscaled scatterers are discussed.</P>