Etiology and clinical characteristics of fever of unknown origin in children: a 15-year experience in a single center

Kim, Yi-Seul,Kim, Kyung-Ran,Kang, Ji-Man,Kim, Jong-Min,Kim, Yae-Jean The Korean Pediatric Society 2017 Clinical and Experimental Pediatrics (CEP) Vol.60 No.3

Purpose: Fever is one of the most common symptoms in children. In previous studies, infectious disease was the most common cause of pediatric fever of unknown origin (FUO). The aim of this study is to investigate the etiology, clinical characteristics and prognosis of pediatric FUO in 21 century with more diagnostics available and to analyze the factors for certain disease categories. Methods: Among the children under 18 years old who were hospitalized at Samsung Medical Center from January 2000 to December 2014, the patients who met the criteria including fever of ${\geq}38.0^{\circ}C$ for longer than ${\geq}14days$ and failure to reach a diagnosis after one week of investigations were included. Results: Total 100 patients were identified. Confirmed diagnosis was achieved in 57 patients (57%). Among them, infectious diseases (n=19, 19%) were most common, followed by connective tissue diseases (n=15, 15%), necrotizing lymphadenitis (n=8, 8%), and malignancies (n=7, 7%). Children with fever duration over 28 days had a trend for higher frequency of connective tissue diseases (28.3%) except undiagnosed etiology. The symptoms such as arthritis, lymph node enlargement and only fever without other symptoms were significantly related with connective tissue diseases, necrotizing lymphadenitis and undiagnosed respectively (P<0.001). Ninety-two patients have become afebrile at discharge and 1 patient died (1%). Conclusion: Almost half of our patients were left without diagnosis. Although it has been known that infectious disease was most common cause of pediatric FUO in the past, undiagnosed portion of FUO have now increased due to development of diagnostic techniques for infectious diseases.

Optimization of Deodeok Extraction Condition Using Response Surface Methodology

Yi-Seul Kim,In-Ho Kim,In-Ju Eom,Seong-Ho Kim 한국식품영양과학회 2017 한국식품영양과학회 학술대회발표집 Vol.2016 No.10

Analyses of Bacterial Communities in Meju, a Korean Traditional Fermented Soybean Bricks, by Cultivation-Based and Pyrosequencing Methods

Yi-Seul Kim,김민철,권순우,Soo-Jin Kim,In-Cheol Park,가종억,원항연 한국미생물학회 2011 The journal of microbiology Vol.49 No.3

Despite the importance of meju as a raw material used to make Korean soy sauce (ganjang) and soybean paste (doenjang), little is known about the bacterial diversity of Korean meju. In this study, the bacterial communities in meju were examined using both culture-dependent and independent methods in order to evaluate the diversity of the bacterial population. Analyses of the 16S rRNA gene sequences of the bacterial strains isolated from meju samples showed that the dominant species were related to members of the genera Bacillus, Enterococcus, and Pediococcus. The community DNAs extracted from nine different meju samples were analyzed by barcoded pyrosequencing method targeting of the V1 to V3 hypervariable regions of the 16S rRNA gene. In total, 132,374 sequences, with an average read length of 468 bp, were assigned to several phyla, with Firmicutes (93.6%) representing the predominant phylum, followed by Proteobacteria (4.5%) and Bacteroidetes (0.8%). Other phyla accounted for less than 1% of the total bacterial sequences. Most of the Firmicutes were Bacillus and lactic acid bacteria, mainly represented by members of the genera Enterococcus, Lactococcus, and Leuconostoc, whose ratio varied among different samples. In conclusion,this study indicated that the bacterial communities in meju were very diverse and a complex microbial consortium containing various microorganisms got involved in meju fermentation than we expected before.

Effects of e-health literacy and oral health knowledge on oral health behavior in adults

Yi Seul Kim,Soon Ryun Lim 한국치위생학회 2022 한국치위생학회지 Vol.22 No.1

Objectives: The purpose of this study was to identify new variables that can enhance adult oral health behaviors by confirming the degree of adult e-health literacy, oral health knowledge, and oral health behaviors and examining their relevance. Methods: A self-reported questionnaire was filled out by 350 adults from June 22 to August 1, 2021. Data were analyzed using SPSS 23.0. independent t-test, one way ANOVA, the scheffé post-hoc test and the pearson correlation coefficients were reviewed, A hierarchical regression analysis was conducted. Results: Oral health behaviors according to general characteristics showed significant differences in gender, educational background, dental visit within 1 year, subjective oral health status, oral health interest, frequency of oral internet use, and reliability of internet oral health information. Also it was found that e-health literacy affects oral health behavior. Conclusions: In this study, e-health literacy, oral health knowledge, and oral health behavior were correlated and it was confirmed that e-health literacy had an effect on oral health behavior. In the future, it is necessary to develop a tool that can measure e-oral health literacy and to find a way to improve the oral health behavior of adults by using e-oral health literacy.

Identification of two acetylcholinesterases in Drosophila suzukii

Yi Seul Kim,Young Ho Kim 한국응용곤충학회 2017 한국응용곤충학회 학술대회논문집 Vol.2017 No.10

Most insects possess two different acetylcholinesterases (AChEs) loci (ace1 and ace2). In contrast, cyclorrhaphan flies,including Drosophila flies, are known to have only a single ace2 locus according to the previous studies. However, sequenceinformation of two different ace genes of Drosophila suzukii have been reported in GenBank. To identify two ace genes(Dsace-A and Dsace-B) in D. suzukii, therefore, we extracted total RNA from 6 individuals and two ace genes successfullywere amplified using gene-specific primers, suggesting that two Dsace genes are transcribed. According to the amino acidsequence analysis, DsAChE-A possesses typical amino acids for 3D structure and catalytic activity of AChE, while someamino acid residues were not conserved in DsAChE-B, suggesting that DsAChE-A and DsAChE-B may be respectivetrue gene and pseudogene of two ace in D. suzukii.

Characerization of Cross-Linked Enzyme Aggregates of Acyl-CoA thioesterase1 precursor from Listeria innocus CLIP 11262

Seul Gi KIM,Song Yi BAE,Nam Yong KIM,So Hyun PARK,T. Doohun KIM 한국생물공학회 2010 한국생물공학회 학술대회 Vol.2010 No.10

Rhodanobacter umsongensis sp. nov., Isolated from a Korean Ginseng Field

Yi-Seul Kim,김수진,Rangasamy Anandham,원항연,권순우 한국미생물학회 2013 The journal of microbiology Vol.51 No.2

A bacterial isolate designated GR24-2T was isolated from Korean soil used for cultivating ginseng (Panax ginseng C. A. Meyer). The strain was aerobic, Gram-negative, motile,and rod-shaped. It grew optimally at 28–30°C, pH 7.0, and in a range of 0–1% NaCl. Phylogenetically, the strain clustered with members of the genus Rhodanobacter. The strain exhibited the highest sequence similarities (>98%) with R. panaciterrae LnR5-47T (98.4%), R. soli DCY45T (98.2%), and R. ginsengisoli GR17-7T (98.0%). However, it also showed high sequence similarities (>97%) with some other Rhodanobacter and Dyella species. The strain contained Q-8 as the predominant respiratory quinone. The major fatty acids (greater than 10% of the total fatty acids) were iso-C17:1 ω9c (24.5%), iso-C16:0 (22.8%), anteiso-C15:0 (10.5%), and iso-C15:0(10.1%). Its major polar lipids were phosphatidylethanolamine,phosphatidylglycerol, diphosphatidylglycerol, and an unknown aminophospholipid. The DNA G+C content of strain GR24-2T was 65.6 mol%. The strain showed less than 70% DNA relatedness values between the closely related Rhodanobacter and Dyella species. The phylogeny, phenotype,DNA–DNA hybridization, and chemotaxonomic data generated in this study reveal that the isolate is a novel species of the genus Rhodanobacter. The name proposed for this strain is Rhodanobacter umsongensis sp. nov. (type strain GR24-2T =KACC 12917T =DSM 21300T).

Anti-obesity effect of Burdock Vinegar on High Fat Diet Fed Rats

Yi-Seul Kim,In-Ho Kim,In-Ju Eom,Seong-Ho Kim 한국식품영양과학회 2017 한국식품영양과학회 학술대회발표집 Vol.2016 No.10

Parapedobacter luteus sp. nov. and Parapedobacter composti sp. nov., isolated from cotton waste compost

Kim, Soo-Jin,Weon, Hang-Yeon,Kim, Yi-Seul,Yoo, Seung-Hee,Kim, Byung-Yong,Anandham, Rangasamy,Kwon, Soon-Wo Microbiology Society 2010 International journal of systematic and evolutiona Vol.60 No.8

<P>Two aerobic, Gram-reaction-negative, non-spore-forming bacterial strains, 4M29<SUP>T</SUP> and 4M40<SUP>T</SUP>, were isolated from cotton composts. The two strains grew in the presence of 0-5 % (w/v) NaCl (optimum growth in the absence of NaCl), at pH 6.0-8.0 (optimum, pH 7.0) and at 15-45 °C (optimum, 30 °C). The strains shared 97.1 % 16S rRNA gene sequence similarity. Strains 4M29<SUP>T</SUP> and 4M40<SUP>T</SUP> showed the next highest levels of 16S rRNA gene sequence similarity to <I>Parapedobacter koreensis</I> Jip14<SUP>T</SUP> (95.6 and 94.4 %, respectively) and <I>Parapedobacter soli</I> DCY14<SUP>T</SUP> (95.2 and 93.8 %). The level of DNA-DNA relatedness between strains 4M29<SUP>T</SUP> and 4M40<SUP>T</SUP> was 38 %. The two strains contained iso-C15 : 0, summed feature 3 (comprising iso-C15 : 0 2-OH and/or C16 : 1<I>ω</I>7<I>c</I>) and iso-C17 : 0 3-OH as major fatty acids, MK-7 as the major respiratory quinone, homospermidine as the only polyamine and phosphatidylethanolamine as the major polar lipid. The DNA G+C contents of strains 4M29<SUP>T</SUP> and 4M40<SUP>T</SUP> were 47.6 and 48.6 mol%, respectively. On the basis of phylogenetic and phenotypic data, strains 4M29<SUP>T</SUP> and 4M40<SUP>T</SUP> are considered to represent two novel species of the genus <I>Parapedobacter</I>, for which the names <I>Parapedobacter luteus</I> sp. nov. (type strain 4M29<SUP>T</SUP> =KACC 10955<SUP>T</SUP> =JCM 15977<SUP>T</SUP>) and <I>Parapedobacter composti</I> sp. nov. (type strain 4M40<SUP>T</SUP> =KACC 10972<SUP>T</SUP> =JCM 15978<SUP>T</SUP>) are proposed.</P>

PRMT7 deficiency causes dysregulation of the HCN channels in the CA1 pyramidal cells and impairment of social behaviors

Seul-Yi Lee,Tuan Anh Vuong,Hyun-Kyung So,Hyun-Ji Kim,Yoo Bin Kim,Jong-Sun Kang,Ilmin Kwon,Hana Cho 생화학분자생물학회 2020 Experimental and molecular medicine Vol.52 No.-

HCN channels regulate excitability and rhythmicity in the hippocampal CA1 pyramidal cells. Perturbation in the HCN channel current (Ih) is associated with neuropsychiatric disorders, such as autism spectrum disorders. Recently, protein arginine methyltransferase 7 (PRMT7) was shown to be highly expressed in the hippocampus, including the CA1 region. However, the physiological function of PRMT7 in the CA1 neurons and the relationship to psychiatric disorders are unclear. Here we showed that PRMT7 knockout (KO) mice exhibit hyperactivity and deficits in social interaction. The firing frequency of the CA1 neurons in the PRMT7 KO mice was significantly higher than that in the wild-type (WT) mice. Compared with the WT CA1 neurons, the PRMT7 KO CA1 neurons showed a more hyperpolarized resting potential and a higher input resistance, which were occluded by the Ih-current inhibitor ZD7288; these findings were consistent with the decreased Ih and suggested the contribution of Ih-channel dysfunction to the PRMT7 KO phenotypes. The HCN1 protein level was decreased in the CA1 region of the PRMT7 KO mice in conjunction with a decrease in the expression of Shank3, which encodes a core scaffolding protein for HCN channel proteins. A brief application of the PRMT7 inhibitor DS437 did not reproduce the phenotype of the PRMT7 KO neurons, further indicating that PRMT7 regulates Ih by controlling the channel number rather than the open probability. Moreover, shRNA-mediated PRMT7 suppression reduced both the mRNA and protein levels of SHANK3, implying that PRMT7 deficiency might be responsible for the decrease in the HCN protein levels by altering Shank3 expression. These findings reveal a key role for PRMT7 in the regulation of HCN channel density in the CA1 pyramidal cells that may be amenable to pharmacological intervention for neuropsychiatric disorders.