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Mitochondrial Genome of Oriental Fruit Moth, Grapholita molesta
Yerim Son,Yonggyun Kim 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.05
Mitochondrial genome is inherited in maternal origin without recombination by mating and its specific regions have been used to monitor insect pest populations in agriculture. The oriental fruit moth, Grapholita molesta, is a serious pest on apple industry by its direct damage on fruits. This study reports a full sequence of mitochondrial genome of G. molesta. Sequence contigs were made by primary PCRs on conserved regions and subsequent PCRs to fill the gaps. Annotated genes were highly matched to the sequences of other lepidopteran species. However, a few positions of tRNA genes on the genome were different to other mitochondrial genomes.
Yerim Son,Jihyun Hwang,김용균 한국응용곤충학회 2012 Journal of Asia-Pacific Entomology Vol.15 No.1
Apolipophorin III (ApoLpIII) plays a crucial role in lipid transport in insects. Its property of binding to lipid moieties is also used in pathogen recognition, such as its binding to lipopolysaccharides of Gram-negative bacteria. This study identified the cDNA sequence of ApoLpIII from the beet armyworm, Spodoptera exigua. Its cDNA was cloned to be 692 bp and it encoded 188 amino acid residues. The predicted amino acid sequence contained a signal peptide (17 residues) at the amino terminal region and one N-glycosylation site. Its predicted molecular weight was 19,042.18 Da after cleavage of the signal peptide. Secondary structure analysis of ApoLpIII predicted that 85.38% of the protein was composed of five α helix domains. RT-PCR analysis showed that ApoLpIII transcript was expressed in all developmental stages, but its expression was restricted to fat body and hemocyte in S. exigua. The expression of ApoLpIII was highly upregulated by bacterial or fungal challenges based on quantitative RT-PCR analysis. When the expression of ApoLpIII was suppressed by injection of its specific dsRNA, the treated larvae showed significant immunosuppression, which was analyzed by hemocyte nodule formation against bacterial challenge in S. exigua. In addition, larvae treated with the specific dsRNA showed high mortality during development. These results suggest that ApoLpIII is associated with development and immune responses in S. exigua.
Yerim Son,Yonggyun Kim 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05
As an environment-friendly phytosanitary measure, CATTS (controlled atmosphere temperature treatment system) has been developed to kill several quarantine insect pests infesting subtropical agricultural commodities. This study tested any possibility to apply CATTS to apples to effectively eliminate the peach fruit moth, Carposina sasakii, which has been regarded as a quarantine insect from the imported countries. When the larvae of C. sasakii were directly exposed to 46℃ (an installed lethal temperature of CATTS), they showed a median lethal time at 14.66 min. Addition of high carbon dioxide to the temperature treatment enhanced the thermal limit susceptibility of C. sasakii to 46℃. The larvae internally infesting apples were tested using this CATTS device and showed 100% lethality after 60 min exposure to a treatment of 46℃ under 15% CO2 in the chamber. This study suggests a possibility that CATTS can be applied as a quarantine measure to kill the larvae of C. sasakii locating inside the apples. To understand the CATTS effect, a heat shock protein was cloned. Hsp90 was partially sequenced and showed its expression in response to heat treatment. CATTS was likely to suppress hsp90 expression.
Yonggyun Kim,Yerim Son,Junga Park,Sungwoo Bae 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.05
Local and seasonal populations of the oriental fruit moth, Grapholita molesta, were monitored with sex pheromone trapping and RAPD (random amplified polymorphic DNA) molecular marker to analyze their movement in apple orchards. To detect their movements among farms, pheromone traps were placed at regions between apple farms ('outside-farms') as well as within-farms ('inside-farms'). Four seasonal adult peaks were evident in apple-cultivating fields from April to October in both trappings of inside- or outside-farms. After overwintering generation, populations of inside-farms were significantly reduced with frequent insecticide applications, compared to populations of outside-farms. Within apple farms, G. molesta tended to be unevenly distributed because of significant sublocal preference. Active movements of local and seasonal populations of G. molesta were supported by gene flow analysis using RAPD marker. Monitoring data using sex pheromone and seasonal reduction in initial genetic differentiation detected in the overwintering populations suggest that there must be significant movement of G. molesta among different orchards in apple-cultivating areas.
Yonggyun Kim,Samyeol Seo,Yerim Son 한국응용곤충학회 2012 한국응용곤충학회 학술대회논문집 Vol.2012 No.05
Sound treatments have been considered as a non-chemical insect pest control technique. Different frequency and intensity sounds were applied to immune and adult stages to screen any stress sounds to alter physiological processes. At 95 dB, 5,000 Hz and 30,000 Hz were selected to be stress sounds in audible and inaudible sound ranges, respectively. Both stress sounds significantly inhibited larval and pupal development. In biochemical analyses, lipid and sugar levels in plasma signigicantly increased in response to the stress sound treatments. Moreover, a digestive phospholipase A2 enzyme activity in midgut was significantly reduced. In adult stage, ultrasound treatment significantly inhibited mating behavior, which resulted in a reduced fecundity. These stress sounds altered gene expressions of stress-related genes, such as heat-shock proteins and apolipophorin III. This study suggests that extreme sounds play a role in physiological stress factors in S. exigua by altering developmental and reproductive processes.
Diagnostic Molecular Markers Against Internal Apple Feeding Insects
Chung Ryul Jung,Yerim Son,Yonggyun Kim 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.05
Three insect pests internally feed pome fruits in Korea. These include oriental fruit moth (Grapholita molesta), plum fruit moth (Grapholita dimorpha) and peach fruit moth (Carposina sasakii). Molecular markers discriminating these three species were developed using PCR-RFLP technique. Mitochondrial (mt) genomes were analyzed to locate polymorphic loci. Six mtDNA regions (CO-Ⅰ, CO-Ⅱ, CB, 16SrRNA-12SrRNA, ND3, ND4) of G. dimorpha were cloned and sequenced. These six sequences of G. dimorpha were aligned with those of C. sasakii and G. molesta to determine polymorphic restriction sites. Predicted PCR-RFLP markers were confirmed with known insect samples. With the validated PCR-RFLP markers, field male adults collected in traps baited with rubber sept lures impregnated with different ratios of major sex pheromone components of G. molesta were analyzed.