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Sang-Kyu Ye,신민경,Eun Hee Yi,김병학,Jae-Cheon Shin,Jung-Youl Park,Chung-Hyun Cho,Jong-Wan Park,최강열 한국분자세포생물학회 2016 Molecules and cells Vol.39 No.11
The -catenin functions as an adhesion molecule and a component of the Wnt signaling pathway. In the absence of the Wnt ligand, -catenin is constantly phosphorylated, which designates it for degradation by the APC complex. This process is one of the key regulatory mechanisms of -catenin. The level of -catenin is also controlled by the E3 ubiquitin protein ligase SIAH-1 via a phosphorylation-independent degradation pathway. Similar to -catenin, STAT3 is responsible for various cellular processes, such as survival, proliferation, and differentiation. However, little is known about how these molecules work together to regulate diverse cellular processes. In this study, we investigated the regulatory relationship between STAT3 and -catenin in HEK293T cells. To our knowledge, this is the first study to report that -catenin-TCF-4 transcriptional activity was suppressed by phosphorylated STAT3; furthermore, STAT3 inactivation abolished this effect and elevated activated -catenin levels. STAT3 also showed a strong interaction with SIAH-1, a regulator of active -catenin via degradation, which stabilized SIAH-1 and increased its interaction with -catenin. These results suggest that activated STAT3 regulates active -catenin protein levels via stabilization of SIAH-1 and the subsequent ubiquitin-dependent proteasomal degradation of -catenin in HEK293T cells.
( Sung Woo Moon ),( Ji Ye Jung ),( Young Ae Kang ),( Moo Suk Park ),( Young Sam Kim ),( Se Kyu Kim ),( Joon Chang ),( Hyo Chae Park ),( Chang Young Lee ),( Song Yee Kim ) 대한결핵 및 호흡기학회 2014 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.118 No.-
Background: Primary graft dysfunction (PGD) is a severe type of acute lung injury after lung transplantation and is reported to be associated with early morbidity and mortality. We were to investigate donor, recipient, and perioperative risk factors and outcome of PGD. Methods: We performed a retrospective study using data collected in one tertiary care hospital in South Korea. The patients who underwent lung transplantation between January 2010 and March 2014 were enrolled. The primary outcome was grade 3 PGD (PaO2/FiO2 < 200 and presence of diffuse infiltrates on chest radiograph of allograft at 48 or 72 hours after transplant) and PGD grade was defined according to International Society for Heart and Lung Transplantation criteria. Results: A total of 61 patients were enrolled and 16 subjects (26.2%) developed grade 3 PGD. In univariate study, higher body mass index (BMI) in recipients, any history of recipient smoking, extracorporeal membrane oxygenation usage before transplantation in recipients, and ischemic time during operation were associated with PGD. Primary recipient diagnosis, transplant type, mean pulmonary artery pressure, donor-smoking history were not related with PGD. In multivariate model, independent risk factors for PGD were BMI in recipients (Odds ratio [OR], 1.290; P=0.048) and total ischemic time during operation (OR, 1.013; P=0.009). PGD was significantly associated with higher re-operation rate (OR, 3.500; P=0.042), longer days of ventilator apply (median 6 days vs.14.5 days; P=0.044), longer intensive care unit stay (median 9 days vs. 17 days; P=0.041) and higher rate of renal replacement therapy (OR, 7.708; P=0.002) after transplantation. Conclusions: We identified risk factors and outcome of grade 3 PGD after lung transplantation. Our findings can be used to develop predictive models for PGD that may allow for modification of risk factors.
In Vivo Inhibition of Bone Morphogenetic Protein-2 on Breast Cancer Cell Growth
Ye, Shuai,Park, Byung-Hyun,Song, Kyung-Jin,Kim, Jung-Ryul,Jang, Kyu-Yun,Park, Ho-Sung,Bae, Jun Sang,Brochmann, Elsa J.,Wang, Jeffrey C.,Murray, Samuel S.,Lee, Kwang-Bok Lippincott Williams Wilkins, Inc. 2013 1528-1159) Vol.38 No.3
STUDY DESIGN.: In vitro and in vivo study. OBJECTIVE.: To evaluate the role of recombinant human bone morphogenetic protein-2 (rhBMP2) on breast cancer cell (MDA-MB-231 cells) growth. SUMMARY OF BACKGROUND DATA.: Bone morphogenetic proteins (BMPs) are expressed in a variety of human carcinoma cell lines and are known to promote tumor invasion and metastasis. However, their roles in tumor progression have not been fully clarified. In addition, there is no in vivo study regarding the inhibitory effect of BMP2 on breast cancer cell proliferation. METHOD.: Cell proliferation was determined by BrdU incorporation assay and flow cytometry. BMP2 signal transduction pathways were estimated on Western blot. Fifteen animals were divided into 2 groups; 1 (control = 5) was breast cancer cells alone, while the other (experiment = 5) was rhBMP2 + breast cancer cells. Cancer cells were injected into 2 sites (subcutaneous and femur) of nude mice with or without BMP2. Tumor size was determined by direct measurements for subcutaneous tumor formation and by femur radiographs. Histological and immunohistochemical analyses were performed. RESULTS.: RhBMP2 inhibited the proliferation of MDA-MB-231 cells in vitro. Inhibition was associated with changes in both the Smad and Wnt signaling pathways and was ultimately mediated through effects on various cell cycle proteins. Furthermore, rhBMP2 inhibited the growth of MDA-MB-231 cells injected both subcutaneously and intrafemorally. CONCLUSION.: In this model using human breast adenocarcinoma cell line, rhBMP2 has no stimulatory effect of tumor growth. Therefore, we can provide the basic science data to support the utilization in the management of patients with spine tumor in the future.
( Sung Woo Moon ),( Kyung Soo Chung ),( Ji Ye Jung ),( Young Ae Kang ),( Moo Suk Park ),( Young Sam Kim ),( Joon Chang ),( Se Kyu Kim ),( Hyo Chae Paik ),( Jun Won Cheong ),( Song Yee Kim ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Introduction: Hemophagocytic lymphohistiocytosis (HLH) is a rare but life-threatening complication after solid organ transplantation. HLH has been reported as problem related with kidney and liver transplant, and there are limited reports of HLH after lung transplantation. Case: A 60-year-old man with idiopathic pulmonary fibrosis underwent bilateral lung transplantation. After lung transplantation, acute rejection was suspected and high dose steroid therapy was done. Since postoperative day(POD) 25, thrombocytopenia( platelet 112*1000/uL) and leukopenia(2530/uL)) were presented. The patient complained of intermittent symptom of low-grade fever, chest discomfort and dyspnea. Echocardiography showed stress induced cardiomyopathy and results of peripheral blood smear was nonspeci. c. Pneumonia was developed and patient was treated with antibiotics. Hyperbilirubinemia(total bilirubin 2.1 mg/dL) started to present at POD 50. Results of abdomen sonography was nonspeci. C except for mild splenomegaly (11.4cm). By POD 80, bilirubin was getting higher(total bilirubin 33.3mg/dL, direct bilirubin 25.7mg/dL, gamma glutamyl transpeptidase 260IU/L) and leukopenia and thrombocytopenia was getting aggravated (white blood cell 2080/uL and platelet 57* 1000/uL). The . brinogen was mildly elevated (4210m/dL), triglyceride was normal (127 mg/dL), the ferritin was elevated (4518 ng/mL) and soluble interleukin-2 receptor was elevated (8730U/ml). However, the finding of peripheral blood smear was still nonspeci. c. The cause of pancytopenia, low grade fever and hyperbilirubinemia was unclear, and we conducted bone marrow biopsy on POD 82. The finding showed that histiocytes were frequently seen with occasional hemophagocytes. Taken together, cytopenia, bone marrow hemophagocytes, elevated soluble interleukin-2 receptor and elevated ferritin were positive among laboratory tests listed in diagnostic criteria of HLH. We managed with etoposide and high dose steroid, but patient deteriorated and died on POD 87. Summary: HLH is a significant diagnostic and therapeutic challenge in lung transplantation and is potentially lethal complication. Therefore, clinicians should consider HLH as possible diagnosis in clinical context.
Development of a method to produce functional chimeric genes via single-strand annealing pathway
Kyu-Jin Ahn,Ju-Hee Choi,Ye-Seul Lim,Min-Ku Kim,Sung-Ho Bae 한국미생물학회 2020 미생물학회지 Vol.56 No.4
Duplicated genes with similar sequences are commonly observed in eukaryotic genomes, some of which show sufficient homology to allow for recombination. ASN1 and ASN2 are an example of duplicated genes that share highly homologous sequence throughout the open reading frame. In this study, we devised a genetic method to produce functional chimeric ASN genes via single-strand annealing pathway. Creation of a DNA doublestrand break between ASN1 and ASN2 using HO endonuclease generated mutant cells with chimeric genes at significantly high frequency. All mutant cells exhibited normal growth in the medium lacking asparagine, suggesting that the chimeric genes are functional. Sequence analysis of chimeric genes revealed that they had different single junctions. Our results provide a method to produce a variety of chimeric genes simultaneously by randomly fusing homologous genes.
( Sung Woo Moon ),( Ji Ye Jung ),( Young Ae Kang ),( Moo Suk Park ),( Young Sam Kim ),( Se Kyu Kim ),( Joon Chang ),( Hyo Chae Park ),( Chang Young Lee ),( Song Yee Kim ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: Primary graft dysfunction (PGD) is a severe type of acute lung injury after lung transplantation and is reported to be associated with early morbidity and mortality.We were to investigate donor, recipient, and perioperative risk factors and outcome of PGD. Methods: We performed a retrospective study using data collected in one tertiary care hospital in South Korea. The patients who underwent lung transplantation between January 2010 and March 2014 were enrolled. The primary outcome was grade 3 PGD (PaO2/ fiO2 < 200 and presence of diffuse infi ltrates on chest radiograph of allograft at 48 or 72 hours after transplant) and PGD grade was defi ned according to International Society for Heart and Lung Transplantation criteria. Results: A total of 61 patients were enrolled and 16 subjects (26.2%) developed grade 3 PGD. In univariate study, higher body mass index (BMI) in recipients, any history of recipient smoking, extracorporeal membrane oxygenation usage before transplantation in recipients, and ischemic time during operation were associated with PGD. Primary recipient diagnosis, transplant type, mean pulmonary artery pressure, donor-smoking history were not related with PGD. In multivariate model, independent risk factors for PGD were BMI in recipients (Odds ratio [OR], 1.290; P=0.048) and total ischemic time during operation (OR, 1.013; P=0.009). PGD was signifi cantly associated with higher re-operation rate (OR, 3.500; P=0.042), longer days of ventilator apply (median 6 days vs.14.5 days; P=0.044), longer intensive care unit stay (median 9 days vs. 17 days; P=0.041) and higher rate of renal replacement therapy (OR, 7.708; P=0.002) after transplantation. Conclusions: We identifi ed risk factors and outcome of grade 3 PGD after lung transplantation. Our fi ndings can be used to develop predictive models for PGD that may allow for modifi cation of risk factors.
Multiple Cavitary Pulmonary Nodules Caused by Mycobacterium intracellulare
Sang Hoon Yoo,Seo Ree Kim,Joon Young Choi,Jae Woo Choi,Yu Mi Ko,Sun Hee Jang,Jun Kyu Park,Ye Gyu Sung,Yun Jung Park,Su Yun Oh,Se Young Bahk,Ju Hyun Lee,Myung Sook Kim 대한가정의학회 2016 Korean Journal of Family Medicine Vol.37 No.4
Nontuberculous mycobacteria (NTM) have been increasingly recognized as an important cause of chronic pulmonary infections. The Mycobacterium avium complex (MAC), which is composed of two species, Mycobacterium avium and Mycobacterium intracelluare, is the most commonly encountered pathogen associated with NTM lung disease. MAC pulmonary infection typically presents in a fibrocavitary form or a nodular bronchiectatic form. However, there have been atypical presentations of MAC pulmonary infections, including solitary pulmonary nodules (SPN). There have been several previous reports of SPN due to MAC infection in the United States, Japan, and Korea. In 2009, Sekine and colleagues reported a case of MAC pulmonary infection presenting with multiple nodules. To date, however, there have been no cases of NTM lung infection with multiple cavitary pulmonary nodules, and neither a fibrotic change nor nodular bronchiectasis. The present case showed a multiple cavitating nodular lung infection due to MAC, which is very rare and different from the typical presentation of MAC pulmonary infections. We also showed that percutaneous transthoracic needle aspiration can be a useful diagnostic tool to evaluate a case of multiple cavitary nodules.