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RISS 인기검색어
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- 저자 : Yazhen Yang (검색결과 3 건)
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Static analysis of thin-walled flexible components in a harmonic reducer
Yazhen Wang,Yang Yu,Wuqiang Jiang,Xiaoyang Chen 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.7
A harmonic reducer is a speed-changing device with thin-walled flexible components. Under the action of a cam wave generator, the flexible components generate controllable elastic deformation to actualize the transfer of motion and power. As two core thin-walled flexible components of the harmonic reducer, the outer ring of flexible bearing (FB) and flexspline (FS) will generate small and continuous deflection waves with the rotation of the wave generator. In this paper, an energy method mechanical model for the FS and the FB in the assembled state is established by theoretical analysis. The internal load distribution of the FB is obtained by solving this model, and the deformation of two flexible components is considered. The effect of the structural parameters of the FS on the load distribution and the strain energy is investigated. The conclusions provide guidance for the structural optimization design of a harmonic drive.
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Mengrui Liu,Da Yin,Baimei Tan,Fan Yang,Xiaoqin Sun,Pengcheng Gao,Shihao Zhang,Yazhen Wang 대한금속·재료학회 2021 ELECTRONIC MATERIALS LETTERS Vol.17 No.1
The adsorption and passivation reactions of 5-methyl benzotriazole (MBTA) with different copper samples (as received, citricacid treated and citric acid and KIO4treated) were studied. The experiments were characterized by contact angle measurement,potentiodynamic polarization curve, electrochemical impedance spectroscopy and X-ray photoelectron spectroscopy. The results showed that the adsorption behavior of MBTA on different treated surfaces was different and MBTA was preferentiallyadsorbed on the surface of citric acid treated copper. Based on the density functional theory, quantum chemicaldescriptors such as the frontier molecular orbital energies EHOMO,ELUMOand the energy gap between them, molecularelectrostatic potential, and Fukui function had been calculated and discussed. The adsorption mechanism of MBTA andcopper surface was further revealed, which had positive significance for the corrosion inhibition of copper surface in copperinterconnection CMP.
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Yongjin Li,Tao Xiong,Huawei Wu,Yazhen Yang 한국바이오칩학회 2016 BioChip Journal Vol.10 No.1
We herein developed a visual DNA microarray system coupled with multiplex PCR (m-PCR) to rapidly detect twelve genetically modified maize (GMM). The microarray comprised short oligonucleotide probes complimentary to the specific gene region for twelve different GMM. The m-PCR products annealed to the microarray probe were reacted with streptavidin-alkaline phosphatase conjugate and nitro blue tetrazolium/5-bromo-4-chloro-3ʹ-indolylphosphate, p-toluidine salt (NBT/BCIP), resulting in blue spots that are easily visualized by unaided eyes for qualitative analysis. To ensure the reliability of this method, positive and negative hybridization controls were used in DNA microarray. Commercial GM materials (GMM: Bt176, Bt11, MON810, GA21, T25,MON88017, NK603, MON863, MON89034, DAS-59122-7, TC1507, MIR604; GM cotton: (MON1445, MON15985); GM soybean (Monsanto Roundup Ready soybean 40-3-2)) and non-GM materials were identified by this method and further confirmed by PCR and sequencing. The results showed that each probe consistently identified its corresponding GMM target very quickly and in a cost-effective and more time efficient way. The limit of detection is 0.5% for Bt176, Bt11, T25, MON88017, DAS59122-7, MON89034 and 1% for MON810, MIR604, GA21, MON863, NK603, TC1507. This method is advantageous because of rapid detection, cost-effectiveness and ease of use. These high specificity and sensitivity results demonstrate the feasibility of using this method in routine analysis of GMOs. We herein developed a visual DNA microarray system coupled with multiplex PCR (m-PCR) to rapidly detect twelve genetically modified maize (GMM). The microarray comprised short oligonucleotide probes complimentary to the specific gene region for twelve different GMM. The m-PCR products annealed to the microarray probe were reacted with streptavidin-alkaline phosphatase conjugate and nitro blue tetrazolium/5-bromo-4-chloro-3ʹ-indolylphosphate, p-toluidine salt (NBT/BCIP), resulting in blue spots that are easily visualized by unaided eyes for qualitative analysis. To ensure the reliability of this method, positive and negative hybridization controls were used in DNA microarray. Commercial GM materials (GMM: Bt176, Bt11, MON810, GA21, T25, MON88017, NK603, MON863, MON89034, DAS- 59122-7, TC1507, MIR604; GM cotton: (MON1445, MON15985); GM soybean (Monsanto Roundup Ready soybean 40-3-2)) and non-GM materials were identified by this method and further confirmed by PCR and sequencing. The results showed that each probe consistently identified its corresponding GMM target very quickly and in a cost-effective and more time efficient way. The limit of detection is 0.5% for Bt176, Bt11, T25, MON88017, DAS59122-7, MON89034 and 1% for MON810, MIR604, GA21, MON863, NK603, TC1507. This method is advantageous because of rapid detection, cost-effectiveness and ease of use. These high specificity and sensitivity results demonstrate the feasibility of using this method in routine analysis of GMOs.
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