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Yongjin Li,Jin-Yu Fan 한국바이오칩학회 2017 BioChip Journal Vol.11 No.1
Considering the factor of culture, religion, health and commerce, the development of a rapid, convenient and sensitive method is important for the detection of the presence of meat species in raw or processed foods. In this study, we employed loop mediated isothermal amplification (LAMP) for species-specific target gene amplification, and the amplicons were analyzed on the immunochromatographic strip (ICS) for the rapid visual identification of meat species. Bovine meat was selected as an example to confirm the performance of such assay. The LAMP based ICS method show good specificity, repeatability and has a limit of detection of 0.1% for beef in meat mixture. The whole detection process could be completed within 50 min. Our method is more convenient and rapid compared to classical LAMP. Moreover, it could be easily adapted to identify other types of meats, and it may be useful for food administration laboratories to carry out meat species identification in raw and processed foods.
Yongjin Li,Tao Xiong,Huawei Wu,Yazhen Yang 한국바이오칩학회 2016 BioChip Journal Vol.10 No.1
We herein developed a visual DNA microarray system coupled with multiplex PCR (m-PCR) to rapidly detect twelve genetically modified maize (GMM). The microarray comprised short oligonucleotide probes complimentary to the specific gene region for twelve different GMM. The m-PCR products annealed to the microarray probe were reacted with streptavidin-alkaline phosphatase conjugate and nitro blue tetrazolium/5-bromo-4-chloro-3ʹ-indolylphosphate, p-toluidine salt (NBT/BCIP), resulting in blue spots that are easily visualized by unaided eyes for qualitative analysis. To ensure the reliability of this method, positive and negative hybridization controls were used in DNA microarray. Commercial GM materials (GMM: Bt176, Bt11, MON810, GA21, T25,MON88017, NK603, MON863, MON89034, DAS-59122-7, TC1507, MIR604; GM cotton: (MON1445, MON15985); GM soybean (Monsanto Roundup Ready soybean 40-3-2)) and non-GM materials were identified by this method and further confirmed by PCR and sequencing. The results showed that each probe consistently identified its corresponding GMM target very quickly and in a cost-effective and more time efficient way. The limit of detection is 0.5% for Bt176, Bt11, T25, MON88017, DAS59122-7, MON89034 and 1% for MON810, MIR604, GA21, MON863, NK603, TC1507. This method is advantageous because of rapid detection, cost-effectiveness and ease of use. These high specificity and sensitivity results demonstrate the feasibility of using this method in routine analysis of GMOs. We herein developed a visual DNA microarray system coupled with multiplex PCR (m-PCR) to rapidly detect twelve genetically modified maize (GMM). The microarray comprised short oligonucleotide probes complimentary to the specific gene region for twelve different GMM. The m-PCR products annealed to the microarray probe were reacted with streptavidin-alkaline phosphatase conjugate and nitro blue tetrazolium/5-bromo-4-chloro-3ʹ-indolylphosphate, p-toluidine salt (NBT/BCIP), resulting in blue spots that are easily visualized by unaided eyes for qualitative analysis. To ensure the reliability of this method, positive and negative hybridization controls were used in DNA microarray. Commercial GM materials (GMM: Bt176, Bt11, MON810, GA21, T25, MON88017, NK603, MON863, MON89034, DAS- 59122-7, TC1507, MIR604; GM cotton: (MON1445, MON15985); GM soybean (Monsanto Roundup Ready soybean 40-3-2)) and non-GM materials were identified by this method and further confirmed by PCR and sequencing. The results showed that each probe consistently identified its corresponding GMM target very quickly and in a cost-effective and more time efficient way. The limit of detection is 0.5% for Bt176, Bt11, T25, MON88017, DAS59122-7, MON89034 and 1% for MON810, MIR604, GA21, MON863, NK603, TC1507. This method is advantageous because of rapid detection, cost-effectiveness and ease of use. These high specificity and sensitivity results demonstrate the feasibility of using this method in routine analysis of GMOs.
On Certain Extension of Hilbert's Integral Inequality with Best Constants
Li, Yongjin,Lin, Yu,He, Bing Department of Mathematics 2008 Kyungpook mathematical journal Vol.48 No.3
In this paper, by introducing a new function with two parameters, we give another generalizations of the Hilbert's integral inequality with a mixed kernel $k(x, y) = \frac {1}{A(x+y)+B{\mid}x-y{\mid}}$ and a best constant factors. As applications, some particular results with the best constant factors are considered.
A New Geometric Constant in Banach Spaces Related to the Isosceles Orthogonality
Yongjin Li,Zhijian Yang 경북대학교 자연과학대학 수학과 2022 Kyungpook mathematical journal Vol.62 No.2
In this paper, starting with the geometric constants that can characterize Hilbert spaces, combined with the isosceles orthogonality of Banach spaces, the orthogo nal geometric constant ΩX(α) is defined, and some theorems on the geometric properties of Banach spaces are derived. Firstly, this paper reviews the research progress of orthogo nal geometric constants in recent years. Then, this paper explores the basic properties of the new geometric constants and their relationship with conventional geometric constants, and deduces the identity of ΩX (α) and γX(α). Finally, according to the identities, the relationship between these the new orthogonal geometric constant and the geometric prop erties of Banach Spaces (such as uniformly non-squareness, smoothness, convexity, normal structure, etc.) is studied, and some necessary and sufficient conditions are obtained.
The Hahn-Banach Theorem on Arbitrary Groups
Huang, Jianfeng,Li, Yongjin Department of Mathematics 2009 Kyungpook mathematical journal Vol.49 No.2
In this paper, one kind of subgroup in arbitrary group which similar to the linear subspace was constructed, and the generalization of the Hahn-Banach theorem on this kind of subgroup in arbitrary groups was obtained.
Approximation of Common Fixed Points of Mean Non-expansive Mapping in Banach Spaces
Gu, Zhaohui,Li, Yongjin Department of Mathematics 2014 Kyungpook mathematical journal Vol.54 No.1
Let X be a uniformly convex Banach space, and S, T be pair of mean nonexpansive mappings. Some necessary and sufficient conditions are given for Ishikawa iterative sequence converge to common fixed points, and we prove that the sequence of Ishikawa iterations associated with S and T converges to the common fixed point of S and T. This generalizes former results proved by Z. Gu and Y. Li [4].
Hyers-Ulam Stability of Pompeiu's Point
Huang, Jinghao,Li, Yongjin Department of Mathematics 2015 Kyungpook mathematical journal Vol.55 No.1
In this paper, we investigate the stability of Pompeiu's points in the sense of Hyers-Ulam.
The Dynamics of Solutions to the Equation $x_{n+1}=\frac{p+x_{n-k}}{q+x_n}+\frac{x_{n-k}}{x_n}$
Xu, Xiaona,Li, Yongjin Department of Mathematics 2010 Kyungpook mathematical journal Vol.50 No.1
We study the global asymptotic stability, the character of the semicycles, the periodic nature and oscillation of the positive solutions of the difference equation $x_{n+1}=\frac{p+x_{n-k}}{q+x_n}+\frac{x_{n-k}}{x_n}$, n=0, 1, 2, ${\cdots}$. where p, q ${\in}$ (0, ${\infty}$), q ${\neq}$ 2, k ${\in}$ {1, 2, ${\cdots}$} and the initial values $x_{-k}$, ${\cdots}$, $x_0$ are arbitrary positive real numbers.
On the Hyers–Ulam Stability of Polynomial Equations in Dislocated Quasi–metric Spaces
Yishi Liu,Yongjin Li 경북대학교 자연과학대학 수학과 2020 Kyungpook mathematical journal Vol.60 No.4
This paper primarily discusses and proves the Hyers–Ulam stability of three types of polynomial equations: x n+a1x+a0 = 0, anxn+· · ·+a1x+a0 = 0, and the infinite series equation: P∞i=0 aix i = 0, in dislocated quasi–metric spaces under certain conditionsby constructing contraction mappings and using fixed–point methods. We present an examplen to illustrate that the Hyers–Ulam stability of polynomial equations in dislocated quasi–metric spaces do not work when the constant term is not equal to zero.