Circ‑NT5C2 stimulates FZD4 expression to promote the malignant progression of osteosarcoma by targeting miR‑488‑3p

Yang Xiaoqi,Wang Shuhua,Zhang Xianjun,Gao Xiangbin,Xu Pengfei 한국응용생명화학회 2022 Applied Biological Chemistry (Appl Biol Chem) Vol.65 No.5

Background: Circ-NT5C2 has been confirmed to be highly expressed and associated to the progression of osteosarcoma (OS). However, the behind mechanism of circ-NT5C2 involvement in OS remains unclear. Methods: The expression of circ-NT5C2, miR-488-3p and FZD4 was measured by quantitative real-time PCR, and the protein expression of E-cadherin, N-cadherin and FZD4 was detected by western blot. Cell counting kit 8 assay, colony formation assay and 5-ethynyl-2-deoxyuridine assay were performed to assess the cell proliferation. The cell apoptosis was measured by flow cytometry and Caspase3/Caspase9 Activity Assay Kits. Cell migration and invasion were detected by transwell assay. Dual-luciferase reporter assay and RIP assay were carried out to determine the binding relation among circ-NT5C2, miR-488-3p and FZD4. Animal experiment and immunohistochemistry analysis were conducted to explore the role of circ-NT5C2 in tumor growth in vivo. Results: Comparing with controls, the expression of circ-NT5C2 and FZD4 was upregulated and miR-488-3p expression was downregulated in OS tumor tissues and cells. Circ-NT5C2 overexpression facilitated the cell proliferation and motility and induced cell apoptosis of OS cells, whereas circ-NT5C2 knockdown had the opposite effect. Besides, we also found and confirmed that circ-NT5C2 regulated cell malignant behaviors via modulating miR-488-3p/FZD4 axis in OS. Moreover, circ-NT5C2 silencing repressed the growth of xenografts in vivo. Conclusion: Circ-NT5C2 upregulated FZD4 expression via sponging miR-488-3p, thus facilitating cell malignant behaviors in OS.

Advanced glycation end products promote meniscal calcification by activating the mTOR-ATF4 positive feedback loop

Yang Sheng,Xie JiaJun,Pan ZhiJie,Guan HongMei,Tu YueSheng,Ye YuanJian,Huang ShouBin,Fu ShiQiang,Li KangXian,Huang ZhiWei,Li XiaoQi,Shi ZhanJun,Li Le,Zhang Yang 생화학분자생물학회 2024 Experimental and molecular medicine Vol.56 No.-

The meniscus is vital for maintaining knee homeostasis and function. Meniscal calcification is one of the earliest radiological indicators of knee osteoarthritis (KOA), and meniscal calcification is associated with alterations in biomechanical properties. Meniscal calcification originates from a biochemical process similar to vascular calcification. Advanced glycation end products (AGEs) and their receptors (RAGEs) reportedly play critical roles in vascular calcification. Herein, we investigated whether targeting AGE-RAGE is a potential treatment for meniscal calcification. In our study, we demonstrated that AGE-RAGE promotes the osteogenesis of meniscal cells and exacerbates meniscal calcification. Mechanistically, AGE-RAGE activates mTOR and simultaneously promotes ATF4 accumulation, thereby facilitating the ATF4-mTOR positive feedback loop that enhances the osteogenic capacity of meniscal cells. In this regard, mTOR inhibits ATF4 degradation by reducing its ubiquitination, while ATF4 activates mTOR by increasing arginine uptake. Our findings substantiate the unique role of AGE-RAGE in the meniscus and reveal the role of the ATF4-mTOR positive feedback loop during the osteogenesis of meniscal cells; these results provide potential therapeutic targets for KOA.

Survey on robotics and automation technologies for civil infrastructure

명현,Yang Wang,Shih-Chung Jessy Kang,XiaoQi Chen 국제구조공학회 2014 Smart Structures and Systems, An International Jou Vol.13 No.6

Over the past several decades, substantial amounts of sensors and sensing systems have beendeveloped for civil infrastructure systems. This special issue focuses on state-of-the-art robotics andautomation technologies, including construction automation, robotics, instrumentation, monitoring,inspection, control, and rehabilitation for civil infrastructure. The issue also covers construction informaticssupporting sensing, analysis and design activities needed to operate smart and sustainable civil infrastructure. Examples include robotic systems applied to civil infrastructure and equipped with various sensingtechnologies, such as optical sensors, laser sensors, wireless sensors, multi-sensor fusion, etc. This specialissue is published in an effort to disseminate current advances of various robotics and automationtechnologies for civil infrastructure and built environment.

PRADA 플래그쉽 스토어 디자인 사례분석을 통한 브랜드 아이덴티티 구축에 관한 연구 - 지역 결합과 문화·예술결합을 중심으로 -

Lin Yang,Su Xiaoqi,이민 세명대학교 인문사회과학연구소 2022 人文 社會科學硏究 Vol.30 No.3

· Research topics: This study analyzes the method of building brand identity using the fashion brand flagship store, focusing on the case of PRADA. · Research background: The homogenization of luxury fashion brands makes it difficult to distinguish products, providing customers with direct experience opportunities · Differences from prior research: Expanded from existing studies considering the design aspect of the flagship store, we analyzed various aspects such as the appearance, exhibition, and design of PRADA, a flagship store best practice brand. · Research method: The factors for building brand identity such as location, appearance, internal design, and exhibition of PRADA flagship store were analyzed from various angles in terms of spatial design. · Research results: The PRADA flagship store designed the appearance suitable for the local environment and integrated with local culture to enhance customer affinity, build a classic image of PRADA, and unify PRADA's luxurious and simple brand image by providing unique symbols and movies · Contribution points and expected effects: The success factors of the existing flagship store case are embodied, and through this, implications are provided to other brands that want to build a brand image using the flagship store.

Construction of graphene/AuNPs based amperometric immunosensor for detecting bladder cancer biomarker apolipoprotein A1

Jing Yang,Wang Xingyuan,Zhou Tingting,Wei Liangjun,Guo Meiling,Liu Yanan,Sun Xiaoqi,Wang Yanjie 한국탄소학회 2024 Carbon Letters Vol.34 No.3

This paper presents the construction and characterization of an amperometric immunosensor based on the graphene/gold nanoparticles (AuNPs/GO) nanocomposite for the detection of the bladder cancer biomarker, apolipoprotein A1 (Apo-A1). The morphological analysis of the AuNPs/GO nanocomposite demonstrated an almost spherical shape of AuNPs and the successful coverage of their surface by graphene oxide. An increased G peak and decreased D peak after the association of AuNPs with GO, implied a reduction in graphene defects. The X-ray photoelectron spectroscopy (XPS) indicated a significant decrease in the quantity of oxygen-containing functional groups in the AuNPs/GO nanocomposite, as compared to the original GO. Furthermore, the developed sensor demonstrated commendable sensitivity and selectivity, with a wide linear range for Apo-A1 detection. Importantly, the immunosensor exhibited remarkable stability over a period of 14 days, signifying its potential for practical applications.

Data-driven Sliding Mode Control for MIMO systems and Its Application on Linear Induction Motors

Dezhi Xu,Xiaoqi Song,Bin Jiang,Weilin Yang,Wenxu Yan 제어·로봇·시스템학회 2019 International Journal of Control, Automation, and Vol.17 No.7

In this paper, a model free adaptive integral sliding mode constrained control (MFAISMCC) is proposedto maintain the flux linkage of a linear induction motor (LIM) while tracking the speed reference. Firstly, themathematica model of a LIM is given after the electrical analysis. In order to linearize the motor system, thetechnology of compact form dynamic linearzation (CFDL) is applied. Then, the MFAISMCC is proposed basedon the analysed linear model. Furthermore, a novel anti-windup compensator for MIMO systems is proposed incase the actuator is within saturation. The whole closed-system stability is proven by Lyapunov stability method. Finally, some simulation results are given to testify the effectiveness of the proposed method compared with thePID controllers.

Survey on robotics and automation technologies for civil infrastructure

Myung, Hyun,Wang, Yang,Kang, Shih-Chung Jessy,Chen, XiaoQi Techno-Press 2014 Smart Structures and Systems, An International Jou Vol.13 No.6

Over the past several decades, substantial amounts of sensors and sensing systems have been developed for civil infrastructure systems. This special issue focuses on state-of-the-art robotics and automation technologies, including construction automation, robotics, instrumentation, monitoring, inspection, control, and rehabilitation for civil infrastructure. The issue also covers construction informatics supporting sensing, analysis and design activities needed to operate smart and sustainable civil infrastructure. Examples include robotic systems applied to civil infrastructure and equipped with various sensing technologies, such as optical sensors, laser sensors, wireless sensors, multi-sensor fusion, etc. This special issue is published in an effort to disseminate current advances of various robotics and automation technologies for civil infrastructure and built environment.

Electronic effects on protective mechanism of electropolymerized coatings based on N-substituted aniline derivatives for mild steel in saline solution

Hao Liu,Baomin Fan,Zining Liu,Xiaoqi Zhao,Biao Yang,Xingwen Zheng,Hua Hao 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.106 No.-

Polyaniline (PANI), poly(N-methylaniline) (PNMA) and poly(N-formylanilide) (PNFA) coatings were fabricatedover mild steel via chronoamperometric strategy in oxalate electrolyte. Scanning electron microscope(SEM), attenuated total reflection infrared spectroscopy (ATR-IR) and X-ray photoelectronspectroscopy (XPS) evidenced the polymerization of monomers. Anticorrosive effects of PANI, PNMAand PNFA coatings for steel substrate were evaluated during long-term immersion in 3.5% NaCl solutionby potentiodynamic polarization, electrochemical impedance spectroscopy (EIS) and frequency modulation(EFM). Morphological variation for coated specimens before and after immersion in NaCl solutionwas also monitored by SEM and atomic force microscope (AFM). Differentiated protection capability wereobserved for three coatings following the sequence of PNFA > PNMA > PANI. Through electrochemical andinterfacial analyses, electroactivity and physical barrier were ascertained as the critical factors, especiallythe former one, in the long-term protection capacity. Electroactivity was distinguished by the elevatedapparent current density from non-destructive EFM measurements. Furthermore, electron-donationand -withdrawal effects of N-substituents played an essential role in coating electroactivity. In detail,N-substituents improved the anodic protection and physical barrier of PNMA and PNFA coatings. Particularly, formyl with electron-withdrawal effect reinforced the anodic protection and thus the superiorthe anticorrosion efficiency of PNFA for underlying steel.