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Radical Scavenging Activity of the Essential Oil of Silver Fir ( <i>Abies alba</i> )
Yang, Seun-Ah,Jeon, Sang-Kyung,Lee, Eun-Jung,Im, Nam-Kyung,Jhee, Kwang-Hwan,Lee, Sam-Pin,Lee, In-Seon the Society for Free Radical Research Japan 2009 Journal of clinical biochemistry and nutrition Vol.44 No.3
<P>The essential oil of silver fir (<I>Abies alba</I>) is known to help respiratory system and have easing and soothing effect for muscle. In the present study, we investigated the chemical composition, cytotoxicity and its biological activities of silver fir (<I>Abies alba</I>) essential oil. The composition of the oil was analyzed by GC-MS and bornyl acetate (30.31%), camphene (19.81%), 3-carene (13.85%), tricyclene (12.90%), dl-limonene (7.50%), α-pinene (2.87%), caryophyllene (2.18%), β-phellandrene (2.13%), borneol (1.74%), bicyclo[2.2.1]hept-2-ene,2,3-dimethyl (1.64%) and α-terpinene (1.24%) were the major components in the oil. The results tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay indicated that the oil showed no cytotoxic effect, at concentrations of 1 and 5%, for as long as 24 and 3 h, respectively. The antiradical capacity was evaluated by measuring the scavenging activity of the essential oil on the 2,20-diphenylpicrylhydrazyl (DPPH) and 2,2'-azino-bis 3-ethyl benzothiazoline-6-sulfonic acid (ABTS) radicals. The oil was able to reduce the both radicals dose-dependently, and the concentration required for 50% reduction (RC<SUB>50</SUB>) against DPPH radicals (2.7 ± 0.63%) was lower than ABTS radicals (8.5 ± 0.27%). The antibacterial activity of the oil was also evaluated using disc diffusion method against <I>Staphylococcus aureus</I>, <I>Streptococcus mutans</I>, <I>Listeria monocytogenes</I>, <I>Acinetobacter baumannii</I>, <I>Escherichia coli</I>, and <I>Vibrio parahaemolyticcus</I>. The oil exhibited no antibacterial activity against all the bacterial strains tested except <I>S. aureus</I> of mild activity.</P>
Comparison of MAST-CLA results in chronic urticaria and atopic dermatitis
( Sang Hyeon Hwang ),( Hyun Min Seo ),( Heun Joo Lee ),( Yoon Hwan Kim ),( Joon Hong Min ),( Joon Lee ),( You Jin Yang ),( Seung Jae Lee ),( Ji Hye Park ),( Chong Won Choi ),( Ga Young Lee ),( Soo Hon 대한피부과학회 2012 대한피부과학회 학술발표대회집 Vol.64 No.3
( Hwan Sung Park ),( Kyung Jo Kim ),( Byong Duk Ye ),( Seung Jae Myung ),( Suk Kyun Yang ) 대한내과학회 2011 대한내과학회 추계학술대회 Vol.2011 No.1
Crohn`s disease (CD) is a chronic inflammatory bowel disease of uncertain etiopathogenesis. Colonoscopy may predict the clinical course in patients with CD. Moreover, periodic colonoscopy is necessary as surveillance for malignancy. We describe here a CD patient with high grade anorectal stricture who successfully underwent total colon examination using an ultra-slim upper endoscope (GIF-XP260N; 5.0 mm ø with instrumental channel 2.0 mm; Olympus, Tokyo, Japan), after failure with standard colonoscope(CF-H260AI; Olympus)and gastroscope (GIF-P230; Olympus). A 37-year-old man, diagnosed with CD in 1999, but had never undergone colonoscopy because of stricture in anorectum. Diarrhea and abdominal pain developed three months before admission. Colonoscopy was performed to assess disease activity and as surveillance for cancer. Three centimeters from the anal verge, however, passage of both standard colonoscope and gastroscope was blocked due to high grade anal stricture. We therefore performed a colonoscopy using an ultra-slim upper endoscope, which passed through the rectal stricture without any resistance. Although other focal strictures were found in transverse (Fig. C)and sigmoid (Fig. D)colon, we succeeded in reaching the cecum(Fig. A) in 3 minutes, but failed to reach the terminal ileum due to narrowed ileocecal valve (Fig. B). Biopsies were taken from the mucosa of the anal stricture to exclude colon cancer. To our knowledge, there have been no reports describing the use of an ultra-slim upper endoscope for colonoscopy in CD patients with strictures.
제주흑우 집단에서 모색 관련 유전자와 microsatellite marker의 다형현상을 이용한 수정란이식 및 인공수정 유래 후대우 검증
Sang-Hyun Han(한상현),Jin-Cheul Ko(고진철),Young-Hoon Kim(김영훈),Kim Nam-Young(김남영),Kim Jae-Hwan(김재환),Moon-Suck Ko(고문석),Ha-Yeon Jeong(정하연),In-Cheol Cho(조인철),Young-Hoon Yang(양영훈),Sung-Soo Lee(이성수) 한국생명과학회 2010 생명과학회지 Vol.20 No.3
농가에 보급된 제주흑우 수정란이식 및 인공수정 생산축의 확인을 위하여 분자유전학적 실험기법을 이용한 개체 추척을 수행하였다. 유전자 marker 체계는 ISAG 권장 MS marker 11종, 예비시험 후 선발된 SAES marker 11종, 흑모색 관련 MC1R과 ASIP 유전자들을 조합하여 분석하였다. 분석결과 부모 정보가 없는 상태에서의 부권 부정율이 국제권장기준보다 높은 수준을 보였으며, 형매간 동일개체출현률은 5.3×10<SUP>-10</SUP>으로 조사되었다. 친자검정 결과 후보축에 대한 후보 부, 모, 부모 모두가 확인되는 경우는 각각 77.0, 54.0, 40.5%였다. 부-모-자간 trio-mismatch가 전혀 없는 수정란이식 개체는 공급 수정란 대비 14.7%로 확인되었고, 전체 후보축군 중 32.4%는 후보부와의 mismatch가 없는 인공수정에 의해 생산된 개체들로 판정하였다. ISAG marker들만을 분석한 결과에서는 7두가 동일한 3가지 유전자형 조합을 나타내었으나, ISAG/SAES marker들을 조합했을 때에는 2두에서만 동일 유전자형 조합을 나타내었다. MS와 모색유전자 분석자료를 모두 조합했을 때는 조사된 모든 개체들이 서로 구분되었다. 현재의 제주흑우집단이 소수 핵군에서 인공수정과 수정란이식 등 생명공학 기법으로 육성된 집단이기에 제주흑우집단의 유전적 다양성은 낮게 나타났다. 본 연구는 유전자 개체식별과 혈통관리 체계의 구축을 위해서는 적어도 20개 이상의 MS marker와 모색관련 유전자형 자료가 필수적으로 활용되어야함을 제안하고 있으며, 연구결과는 향후 제주흑우의 분자육종에 있어 유용한 자료가 될 것으로 시사하고 있다. To find offspring of Jeju Black cattle (JBC) produced by embryo transfer (ET) and artificial insemination (AI), a molecular genetic study was carried out in candidate cattle populations collected from cattle farms in Jeju Island, Korea. The genetic marker set was composed of 11 ISAG microsatellite (MS) markers, 11 SAES MS markers selected by our preliminary analysis for population diversity of JBC, and two major coat color related genes: MC1R and ASIP. The results showed a combined non-exclusion probability for first parent (NE-P1) that was higher than that recommended by ISAG (above 0.9995), and a combined non-exclusion probability for sib identity of 5.3×10<SUP>-10</SUP>. Parentage analysis showed that the cases identified the candidate’s father only (77.0%), mother only (54.0%), and both parents (40.5%) in the candidate offspring population. The ET and AI calves were identified as 14.7% in the in vitro fertilized eggs provided and 32.4% in total population, respectively. However, the result from ISAG marker analysis showed 3 identical allele-combinations in 7 calves, and that from ISAG/SAES MS marker combination also showed 1 identical allele-combination in 2 calves. Data from MS and coat-color gene analyses provided information for complete identification of all animals tested. Because the present JBC population was mostly bred using small nuclear founders through bioengineering techniques such as AI and ET, the genetic diversity levels obtained from MS analysis in the JBC population were relatively lower than those of other cattle populations, including Hanwoo. The results suggested that the more efficient marker combinations, including coat color related genotypes, should be studied and used for constructing a system for identification and molecular breeding of JBC as well.