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      • Analysis of copy number variations in Holstein cows identify potential mechanisms contributing to differences in residual feed intake.

        Hou, Yali,Bickhart, Derek M,Chung, Hoyoung,Hutchison, Jana L,Norman, H Duane,Connor, Erin E,Liu, George E Springer 2012 Functional & integrative genomics Vol.12 No.4

        <P>Genomic structural variation is an important and abundant source of genetic and phenotypic variation. In this study, we performed an initial analysis of copy number variations (CNVs) using BovineHD SNP genotyping data from 147 Holstein cows identified as having high or low feed efficiency as estimated by residual feed intake (RFI). We detected 443 candidate CNV regions (CNVRs) that represent 18.4?Mb (0.6?%) of the genome. To investigate the functional impacts of CNVs, we created two groups of 30 individual animals with extremely low or high estimated breeding values (EBVs) for RFI, and referred to these groups as low intake (LI; more efficient) or high intake (HI; less efficient), respectively. We identified 240 (~9.0?Mb) and 274 (~10.2?Mb) CNVRs from LI and HI groups, respectively. Approximately 30-40?% of the CNVRs were specific to the LI group or HI group of animals. The 240 LI CNVRs overlapped with 137 Ensembl genes. Network analyses indicated that the LI-specific genes were predominantly enriched for those functioning in the inflammatory response and immunity. By contrast, the 274 HI CNVRs contained 177 Ensembl genes. Network analyses indicated that the HI-specific genes were particularly involved in the cell cycle, and organ and bone development. These results relate CNVs to two key variables, namely immune response and organ and bone development. The data indicate that greater feed efficiency relates more closely to immune response, whereas cattle with reduced feed efficiency may have a greater capacity for organ and bone development.</P>

      • KCI등재

        AN ELECTRODE MODIFIED WITH AuNPs/GRAPHENE NANOCOMPOSITES FILM FOR THE DETERMINATION OF METHYL PARATHION RESIDUES

        YALI MAO,HUANBAO FA,YANMEI CHENG,CHANGJUN HOU,Dong Zhang,YANQIU DU,WEI YIN,DANQUN HUO 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2014 NANO Vol.9 No.8

        A novel sensitive and elective electrochemical sensor was developed to detect methyl parathion(MP) based on a glassy carbon electrode (GCE) modified with gold nanoparticles (AuNPs)/graphene nanocomposites film. The AuNPs were modified onto graphene sheets using NaBH4 as a reductant. The obtained AuNPs/graphene nanocomposites were characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The electrochemical behavior of MP and interference studies were then investigated. Compared with metal ions and nitroaromatic compounds, which exist in environmental samples, the AuNPs/graphene/GCE exhibited high adsorption and strong affinity toward MP. Under optimized conditions, the oxidationpeak current of MP was linear to its concentration within the range of 4.0×10-7 –8.0×10-5M, with a detection limit (S/N=3) of 8.5×10-8 M. These results indicated that the AuNPs/graphene nanocomposites displayed a synergic effect involving the catalytic characteristics of graphene and AuNPs nanocomposites, which can effectively improve the electrochemicalproperties of MP. Furthermore, the AuNPs also enhanced sensor sensitivity to MP. Therefore, the AuNPs/graphene/GCE could be a promising sensor for the fast, sensitive and selective detection of MP in real samples.

      • KCI등재

        Antioxidant Enzyme Activity and Chilling Injury during Low-temperature Storage of Kiwifruit cv. Hongyang Exposed to Gradual Postharvest Cooling

        Qingzhen Yang,Jingping Rao,Shunchao Yi,Kun Meng,Jianfeng Wu,Yali Hou 한국원예학회 2012 Horticulture, Environment, and Biotechnology Vol.53 No.6

        Kiwifruits (Actinidia chinensis cv. Hongyang) were treated by direct cooling and gradual cooling to investigate the effect of cooling treatment on chilling injury. The direct cooling fruits were immediately cooled at 0℃ after harvest. The gradual cooling fruits were held for 3 days at 5℃ (from 5℃ to 0℃), or for 7 days at 2℃(from 2℃ to 0℃), or decreased in temperature from 15℃ to 5℃ by 5℃ at 1 days intervals and then maintained at 5℃ for 3 days plus a subsequent period of of 7 days at 2℃ (from 15℃ to 0℃). After the above treatments, then those fruit were stored at 0 ± 0.5℃, 90% to 95% RH for 80 days. Gradual cooling (from 15℃ to 0℃) significantly maintained higher percentage of accepted fruit and lower chilling injury index and chilling injury incidence of fruit compared with the direct cooling. Some attributes were then assayed in the fruits treated with gradual cooling (from 15℃ to 0℃). Gradual cooling (from 15℃ to 0℃) inhibited increases in membrane permeability, malondialdehyde content, superoxide anion production rate, and H2O2 content. At the same time, fruit cooled gradually (from 15℃ to 0℃) exhibited higher superoxide dismutase, catalase, ascorbate peroxidase, and peroxidase activities than those treated by direct cooling during storage. The present study indicated that enhancement in antioxidant enzyme activity may be attributed to the reduction in CI symptoms by the gradual cooling treatment (from 15℃ to 0℃).

      • KCI등재

        Convective Heat Transfer Coeicient Model Under Nanoluid Minimum Quantity Lubrication Coupled with Cryogenic Air Grinding Ti–6Al–4V

        Jianchao Zhang,Wentao Wu,Changhe Li,Min Yang,Yanbin Zhang,Dongzhou Jia,Yali Hou,Runze Li,Huajun Cao,Hafiz Muhammad Ali 한국정밀공학회 2021 International Journal of Precision Engineering and Vol.8 No.4

        Under the threat of serious environmental pollution and resource waste, sustainable development and green manufacturing have gradually become a new development trend. A new environmentally sustainable approach, namely, cryogenic air nanofluid minimum quantity lubrication (CNMQL), is proposed considering the unfavorable lubricating characteristic of cryogenic air (CA) and the deficient cooling performance of minimum quantity lubrication (MQL). However, the heat transfer mechanism of vortex tube cold air fraction by CNMQL remains unclear. The cold air fraction of vortex tubes influences the boiling heat transfer state and cooling heat transfer performance of nanofluids during the grinding process. Thus, a convective heat transfer coefficient model was established based on the theory of boiling heat transfer and conduction, and the numerical simulation of finite difference and temperature field in the grinding zone under different vortex tube cold air fractions was conducted. Simulation results demonstrated that the highest temperature initially declines and then rises with increasing cold air fraction. Afterward, this temperature reaches the lowest peak (192.7 °C) when the cold air fraction is 0.35. Experimental verification was conducted with Ti–6Al–4V to verify the convective heat transfer coefficient model. The results concluded that the low specific grinding energy (66.03 J/mm 3 ), high viscosity (267.8 cP), and large contact angle (54.01°) of nanofluids were obtained when the cold air fraction was 0.35. Meanwhile, the lowest temperature of the grinding zone was obtained (183.9 °C). Furthermore, the experimental results were consistent with the theoretical analysis, thereby verifying the reliability of the simulation model.

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