True Digestibility of Phosphorus in Different Resources of Feed Ingredients in Growing Pigs

Wu, X.,Ruan, Z.,Zhang, Y.G.,Hou, Y.Q.,Yin, Y.L.,Li, T.J.,Huang, R.L.,Chu, W.Y.,Kong, X.F.,Gao, B.,Chen, L.X. Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.1

To determine the true digestible phosphorus (TDP) requirement of growing pigs, two experiments were designed with the experimental diets containing five true digestible P levels (0.16%, 0.20%, 0.23%, 0.26% and 0.39%) and the ratio of total calcium to true digestible P (TDP) kept at 2:1. In Experiment 1, five barrows (Duroc${\times}$Landrace${\times}$Yorkshire) with an average initial body weight of 27.9 kg were used in a $5{\times}5$ Latin-square design to evaluate the effect of different dietary P levels on the digestibility and output of P and nitrogen. In Experiment 2, sixty healthy growing pigs (Duroc${\times}$Landrace${\times}$Yorkshire) with an average body weight (BW) of 21.4 kg were assigned randomly to one of the five dietary treatments (12 pigs/diet), and were used to determine the true digestible phosphorus (TDP) requirement of growing pigs on the basis of growth performance and serum biochemical indices. The results indicated that the true digestibility of P increased (p<0.05) linearly with increasing dietary TDP level below 0.26%. The true P digestibility was highest (56.6%) when dietary TDP was 0.34%. Expressed as g/kg dry matter intake (DMI), fecal P output increased (p<0.05) linearly with increasing P input. On the basis of g/kg fecal dry matter (DM), fecal P output was lowest for Diet 4 and highest (p<0.05) for Diet 5. The apparent digestibility of crude protein (CP) did not differ (p>0.05) among the five diets, with the average nitrogen output of 12.14 g/d and nitrogen retention of 66% to 74% (p>0.05), which suggested that there was no interaction between dietary P and CP protein levels. During the 28-d experimental period of Experiment 2, the average daily gain (ADG) of pigs was affected by dietary TDP levels as described by Eq. (1): $y=-809,532x^4+788,079x^3-276,250x^2+42,114x-1,759$; ($R^2=0.99$; p<0.01; y = ADG, g/d; x = dietary TDP, %), F/G for pigs by Eq. (2): $y=3,651.1x^4-3,480.4x^3+1,183.8x^2-172.5x+10.9$ ($R^2=0.99$; p<0.01; y = F/G; x = dietary TDP, %), and Total P concentrations in serum by Eq. (3): $y=-3,311.7x^4+3,342.7x^3-1,224.6x^2+195.6x-8.7$ (R2 = 0.99; p<0.01; y = total serum P concentration and x = dietary TDP, %). The highest ADG (782 g/d), the lowest F/G (1.07) and the highest total serum P concentration (3.1 mmol/L) were obtained when dietary TDP level was 0.34%. Collectively, these results indicate that the optimal TDP requirement of growing pigs is 0.34% of the diet at a total Ca to TDP ratio of 2:1.

New Evidences of Effect of Melanocortin-4 Receptor and Insulin-like Growth Factor 2 Genes on Fat Deposition and Carcass Traits in Different Pig Populations

Chen, J.F.,Xiong, Y.Z.,Zuo, B.,Zheng, R.,Li, F.E.,Lei, M.G.,Li, J.L.,Deng, C.Y.,Jiang, S.W. Asian Australasian Association of Animal Productio 2005 Animal Bioscience Vol.18 No.11

The Melanocortin-4 Receptor (MC4R) and Insulin-like Growth Factor 2 (IGF2) are two important candidate genes related to fat deposition and carcass traits. MC4R was found on study on human obesity and then was studied as candidate gene affecting food intake and fat deposition traits in mice and pigs. Insulin-like Growth Factor 2 (IGF2) gene plays an important role on tumor cell proliferation and muscle growth. It also affects fat traits and live weight in pigs. In this paper, MC4R and IGF2 were studied as two candidate genes associated with important economic traits such as fat deposition and carcass traits in five different pig populations. Taq I-PCR-RFLP and Bcn I-PCR-RFLP were respectively used to detect the polymorphism of genotypes of MC4R and IGF2 genes. Different MC4R genotype frequencies were observed in four populations. IGF2 genotype frequencies were also different in two populations. The results of association analysis show both MC4R and IGF2 genes were significantly associated with fat deposition and carcass traits in about 300 pigs. This work will add new evidence of MC4R and IGF2 affecting fat deposition and carcass traits in pigs and show that two genes can be used as important candidate genes for marker assistant selection (MAS) of growth and lean meat percentage in pigs.

Dietary Requirement of True Digestible Phosphorus and Total Calcium for Growing Pigs

Ruan, Z.,Zhang, Y.-G.,Yin, Y.-L.,Li, T.-J.,Huang, R.-L.,Kim, S.W.,Wu, G.Y.,Deng, Z.Y. Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.8

Sixty healthy growing pigs ($Duroc{\times}Landrace{\times}Yorkshire$ with an average BW of 21.4 kg) were used to determine the true digestible phosphorus (TDP) requirement of growing pigs on the basis of growth performance and serum biochemical indices. Pigs were assigned randomly to one of five dietary treatments (12 pigs/diet), representing five levels of TDP (0.16%, 0.20%, 0.23%, 0.26% and 0.39%). There were three replications per treatment, with four pigs (2 barrows and 2 gilts) in each replication (2 pigs/pen) A randomized-block design was used, with pen as the experimental unit. Experimental diets were formulated to provide the 5 TDP levels with a total calcium (Ca) to TDP ratio of 2:1, and offered to pigs at 5% BW for 28 d. The total Ca contents of the five diets were 0.33, 0.38, 0.45, 0.51 and 0.79%, respectively. During the 28-d experimental period, the ADG of pigs was affected by dietary TDP levels as described by Equation 1: y = $-809,532x^4+788,079x^3-276,250x^2+42,114x-1$,759; ($R^2$ = 0.99; p<0.01; y = ADG, g/d; x = dietary TDP, %). The feed:gain ratio for pigs was affected by dietary TDP levels as described by Equation 2: y = $3,651.1x^4-3,480.4x^3+1,183.8x^2-172.5x+10.9$ ($R^2$ = 0.99; p<0.01; y = feed:gain ratio; x = dietary TDP, %). Total P concentrations in serum were affected by dietary TDP levels as described by Equation 3: y = $-3,311.7x^4+3,342.7x^3-1,224.6x^2+195.6x-8.7$ ($R^2$ = 0.99; p<0.01; y = total serum P concentration and x = dietary TDP, %). The highest ADG (782 g/d), the lowest feed:gain ratio (1.07), and the highest total serum P concentration (3.1 mmol/L) were obtained when dietary TDP level was 0.34%. Collectively, these results indicate that the optimal TDP requirement of growing pigs is 0.34% of the diet (e.g., 5.1 g/day for a 30-kg pig that consumed 1.5 kg feed daily) at a total Ca to TDP ratio of 2:1.

Loss Test for a 5-m YBCO Cable Sample of the 22.9-kV KEPCO System Under Grid Operation

Li, Z. Y.,Ma, Y. H.,Ryu, K.,Oh, S. R.,Yim, S. W.,Hwang, S. D. IEEE 2013 IEEE transactions on applied superconductivity Vol.23 No.3

<P>We have operated the 22.9-kV high temperature superconductor cable system installed at Icheon Substation of Korea Electric Power Corporation (KEPCO), which consists of a conductor and a shield using YBCO-NiW wires. We prepared the same 5-m cable sample as the Icheon Substation in order to understand its ac loss characteristic. Especially, the contact resistance between copper current leads and YBCO wires was minimized through the increased soldering surface between them. Besides, four voltage leads are attached to both the conductor and the shield with an equal angle of 90°. Under ac transport current, which was supplied to the conductor and shield in opposite direction, losses of the 5-m sample were tested for various contact positions of voltage leads, frequencies, and transport periods. The result shows that the measured ac losses for various contact positions in the conductor and shield are the same. However, their loss exponents are very different. More details will be presented in this work.</P>

An automated focusing method for a parallel micro-manipulator alignment

Li, Z. Y.,Ihn, Y. S.,Choi, H. R.,Moon, H.,Koo, J. C. Springer Science + Business Media 2016 Microsystem Technologies Vol.22 No.6

<P>In this work, particle swarm optimization (PSO) algorithm is employed to align spot array and judging image sharpness with auto-focusing (AF) image evaluation function. To satisfy the requirement of optical alignment system, a high precision manipulator has to be developed. Applied parallel manipulator that consists of four 2-degree of freedom (DOF) decoupled actuator gives chances to provide 6-DOF independence motion, and has strength in high accuracy. This paper covers the follows: Firstly, motivation of this development for the digital mirror device (DMD) based maskless lithography system to introduce spot array method in maskless digital exposure process. Secondly, in order to precisely control moving platform, applying a redundant parallel micro-manipulator. Thirdly, proposing a suitable error model of the system and applying the PSO and Z axis auto-focusing algorithm to align spot array concerning position correction. Finally, we designed experiment which is applied a vision sensor and alignment unit to verify proposed positioning algorithm. Experimental result will be shown that proposed can solve the alignment problem by reducing the position error of the system.</P>

Chemical Synthesis and Silica Encapsulation of NiPt Nanoparticles

Li, Y.,Zhang, X. L.,Qiu, R.,Qiao, R.,Kang, Y. S. American Chemical Society 2007 The Journal of Physical Chemistry Part C Vol.111 No.29

ZO-1 and -2 Are Required for TRPV1-Modulated Paracellular Permeability

Li, J.,Cong, X.,Zhang, Y.,Xiang, R.L.,Mei, M.,Yang, N.Y.,Su, Y.C.,Choi, S.,Park, K.,Zhang, L.W.,Wu, L.L.,Yu, G.Y. SAGE Publications 2015 Journal of dental research Vol.94 No.12

<P>The tight junction based paracellular pathway plays an important role in saliva secretion. Zonula occludens (ZO) proteins are submembranous proteins of tight junction complex; however, their function in salivary epithelium is poorly understood. Here, we found that activation of transient receptor potential vanilloid subtype 1 (TRPV I) by capsaicin increased rat saliva secretion both in vivo and ex vivo. Meanwhile, TRPV I activation enlarged the width of tight junctions between neighboring acinar cells, increased the paracellular flux of 4-kDa fluorescein isothiocyanate (FITC)-dextran in submandibular gland (SMG) tissues, and decreased transepithelial electric resistance (TER) in SMG-C6 cells. ZO-1, -2, and -3 were distributed principally to the apical lateral region of acinar cells in SMG tissues and continuously encircled the peripheries of SMG-C6 cells in the untreated condition. TRPV I activation obviously diminished ZO-1 and -2 staining, but not ZO-3 or f3-catenin, at the cell-cell contacts ex vivo and in vitro. Moreover, in untreated SMG-C6 cells, ZO-1 and -2 single or double knockdown by small interfering RNA (siRNA) increased the paracellular flux of 4-kDa FITC-dextran. In capsaicintreated cells, ZO-1 and -2 single or double knockdown abolished, whereas their re-expression restored, the capsaicin-induced increase in paracellular permeability. Furthermore, TRPV I activation increased RhoA activity, and inhibition of either RhoA or Rho kinase (ROCK) abolished the capsaicin-induced TER decrease as well as ZO-1 and -2 redistribution. These results indicate that ZO-1 and -2 play crucial roles in both basal salivary epithelial barrier function and TRPV I -modulated paracellular transport. RhoA-ROCK signaling pathway is responsible for TRPVI -modulated paracellular permeability as well as ZO-1 and -2 redistribution.</P>

Experimental Study of the Runaway Current in the J-TEXT Tokamak

Y. H. Luo,Z. Y. Chen,X. Q. Zhang,D. W. Huang,W. Jin,Y. H. Huang,Y. Tang,J. C. Li,R. H. Tong,W. Yan,G. Zhuang 한국물리학회 2014 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.64 No.3

Major plasma disruptions in tokamaks often generate runaway currents, which contain electronswith energies of several tens of megaelectron-volts (MeV). These currents can cause substantialdamage when control is lost and the current hits the limiters or the vessel wall. The interactionbetween the runaway electrons and the impurities inside the plasma results in soft X-ray emission,which can provide detailed information about the runaway generation process and the confinementof runaway electrons. A vertical soft X-ray array at the top of Joint Texas Experimental Tokamak(J-TEXT) was used to study the runaway beams resulting from major disruptions. Runawayelectron production and confinement of runaway current were observed by using soft X-ray images.

Field dependence of antiferromagnetic domain switching in epitaxial Fe/CoO/MgO(001) systems

Li, Q.,Ma, T. P.,Yang, M.,Sun, L.,Huang, S. Y.,Li, R. W.,Won, C.,Qiu, Z. Q.,Wu, Y. Z. American Physical Society 2017 Physical Review B Vol.96 No.2

<P>Utilizing the magneto-optic Kerr effect and Kerr microscopy measurements, we investigated the antiferro-magnetic (AFM) domain switching process at different magnetic fields in a single-crystalline Fe/CoO bilayer grown on MgO(001) substrate. In spite of the zero-net magnetic moment in the CoO layer, we find that the activation energy barrier of CoO AFM domain switching decreased at larger magnetic field. To separate the different behaviors of domain nucleation and domain wall motion during the CoO spin switching process, a new analytical method was developed. Using this method, we found that the CoO domain nucleation energy barrier exhibited a jump at a critical magnetic field while the CoO domain wall motion experienced only a tiny energy barrier variation. The field-dependent behaviors of the energy barriers were attributed to the formation of a spiral domain wall in the Fe layer during its magnetization reversal and this was supported by micromagnetic simulations.</P>

First Measurements of Absolute Branching Fractions of the Ξc0 Baryon at Belle

Li, Y. B.,Shen, C. P.,Yuan, C. Z.,Adachi, I.,Aihara, H.,Al Said, S.,Asner, D. M.,Aushev, T.,Ayad, R.,Badhrees, I.,Ban, Y.,Bansal, V.,Beleñ,o, C.,Berger, M.,Bhardwaj, V.,Bhuyan, B.,Bilka, T.,Bisw American Physical Society 2019 Physical review letters Vol.122 No.8