H3K27 methylation and H3S28 phosphorylation-dependent transcriptional regulation by INHAT subunit SET/TAF-Iβ

Kim, J.Y.,Kim, K.B.,Son, H.J.,Chae, Y.C.,Oh, S.T.,Kim, D.W.,Pak, J.H.,Seo, S.B. North-Holland Pub ; Elsevier Science Ltd 2012 FEBS letters Vol.586 No.19

Significant progress has been made in understanding the relationship between histone modifications and 'reader' molecules and their effects on transcriptional regulation. A previously identified INHAT complex subunit, SET/TAF-Iβ, binds to histones and inhibits histone acetylation. To investigate the binding specificities of SET/TAF-Iβ to various histone modifications, we employed modified histone tail peptide array analyses. SET/TAF-Iβ strongly recognized PRC2-mediated H3K27me½/3; however, the bindings were completely disrupted by H3S28 phosphorylation. We have demonstrated that SET/TAF-Iβ is sequentially recruited to the target gene promoter ATF3 after the PRC2 complex via H3K27me recognition and may offer additive effects in the repression of the target gene.

Effects of Egg Yolk Antibodies Produced in Response to Different Antigenic Fractions of E. coli O157:H7 on E. coli Suppression

Chae, H.S.,Singh, N.K.,Ahn, C.N.,Yoo, Y.M.,Jeong, S.G.,Ham, J.S.,Kim, D.W. Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.11

The objective of this research was to provide the characterization and method for producing anti-E. coli O157:H7 antibodies in egg-laying hens and to determine if the antibody can restrain the proliferation of E. coli O157:H7 in-vitro. Selected antigenic fractions (whole cell, outer membrane protein and lipopolysaccharide (LPS)) from E. coli O157:H7 were injected to hens in order to produce anti-E. coli O157:H7 antibodies. The immune response and the egg yolk antibodies of laying hens against the whole cell, outer membrane protein and LPS antigens were monitored by ELISA. The level of antibodies against whole cell antigen monitored through ELISA sharply increased after the initial immunization, and it was found to be maximum on day 49 however, the level was maintained up to day 70. Antibodies (5 mg/ml) directed against the whole cell inhibited E. coli proliferation 10-13 times more than outer membrane protein or LPS. The antibody response against the whole cell antigens appeared to have higher activity in restraining the proliferation of E. coli O157:H7 than antibody against outer membrane protein or LPS. Results reflected that increasing the IgY's in the egg yolk could prevent greater economic losses due to human and animal health from pathogenic bacteria i.e. E. coli O157:H7.

국내 Human Immunodeficiency virus(HIV) 감염의 역학 및 면역학적 특성

신영오,장영식,허숙진,기미경,김성순,최병선,최민호,전원경,채권석,이영종 국립보건연구원 1994 국립보건원보 Vol.31 No.1

원주지역 남은 음식물의 계절별 성분 함량 및 비육돈에 대한 건조 남은 음식물 급여 효과

채병조,주지환,심영호,권일경,김상헌 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.3

본 연구는 남은 음식물(FW)의 성분변이를 조사하고, 그것이 급여시 비육돈의 성장과 육질에 미치는 영향을 조사하기 위하여 실시하였다. 사료화를 위해 기초자료로 활용하고져 FW를 1년간 (월 6회) 수집하였다. 8주간의 사양시험을 위해 삼원교잡종(LxYxD) 비육돈 (54.80±4.60㎏) 117두를 공시하였다 (3처리 3반복, 반복당 13두). 처리는 대조구 (옥수수-대두박 위주사료), 건조 방법에 따른 단순 건조 (simple dry : SD) 및 진공발효 (vacuum fermentation: VF)로서 구분하였다. FW의 건물기준으로 에너지, 조단백질, 조지방, 화분, 칼슘 및 인의 4계절 평균치는 각각 5,11kcal/㎏, 22.92%, 14.31%, 15.48%, 2.7% 및 1.05%였다. 에너지와 단백질 함량은 각각 겨울과 여름에 가장 높았다 (p<0.05). 건조한 FW의 유산균 함량은 SD와 VF 처리간에 차이가 없었다. 사양성적에서는 ADG에서 대조구가 FW이 첨가된 사료에 비해 유의적으로 높았으나 (p<0.05), FCR에서는 처리간에 차이가 없었다. SD와 FD와의 생산성 비교에서는 유의차가 인정되지 않았다. 지육율, 등지방두께, 그리고 기타육질(색깔, drip loss 및 TBARS)에서 처리간에 차이가 없었다. 전 시험기간의 증체 ㎏당 사료비를 살펴보면, FW가 포함된 사료급여군이 대조구에 비해 낮았다. 이상의 결과를 요약해 보면, 남은 음식물을 건조한 후 사료에 20% 이내를 첨가하여 펠렛사료로 급여할 경우 비육돈의 사료비를 절감할 수 있을 것으로 사료된다. 그러나 FW의 가공시 FD공정은 필요치 않을 것으로 사료된다. A study was conducted to evaluate seasonal variations in chemical composition of food waste (FW) and its feeding effects on growth performance and pork quality in finishing pigs. FW was collected for 1 year ( 6 times a month ) to establish a database for use of FW as a feed ingredient. For a feeding trial (8 weeks), a total of 117 pigs (L×Y×D; 54.80±4.60㎏) were used to evaluate the processing effects of FW. Treatments were: Control (a corn-soybean meal diet without FW), simple dried FW (SD) and vacuum fermented FW (VF). The gross energy, crude protein, crude fat, ash, calcium and phosphorus in FW (DM, average of 4 seasons) were 5,111㎉/㎏, 22.92%, 14.31%, 15.48%, 2.7% and 1.05%, respectively. Among seasons, the energy and crude protein contents were the highest (p<0.05) in winter and summer, respectively. In lactic acid bacterial counts, there was no difference between SD and VF. Pigs fed the control diet grew faster (p<0.05) than those fed diets containing food waster, but not feed conversion ratio. There were no differences in production traits between SD and VF. No differences were also found in dressing percentage, backfat thickness, and pork quality (color, drip loss and TBARS) among treatments. The feed cost (￦/㎏ body weight) was lower in pigs fed FW than those fed a control diet. In conclusion, a pelleted diet containing food waste less than 20% would reduce feed cost in finishing pigs. However, it seems that a vacuum fermentation of food waste is not necessary for diet processing.

Effect of an temperatures of post-spray heat treatment on wear behavior of 8% Y₂O₃-ZrO₂ coating

Y. H. Chae,S. S. KIM 한국트라이볼로지학회 2002 한국트라이볼로지학회 학술대회 Vol.2002 No.10

Most recent, Plasma ceramic spray is used on parts of tribosystem, has been investigated on the tribological performance. The application of ceramic coatings by plasma spray has become essential in tribosystems to produce better wear resistance and longer life in various conditions. The purpose of this work was to investigate the wear behavior of 8% Y₂O₃-ZrO₂ coating due to temperatures of post-spay heat treatment. The plasma-sprayed 8% Y₂O₃-Zirconia coating was idiscussed to know the relationship between phase transformations and temperatures of post-spray heat treatment. Wear tests was carried out with ball on disk type on normal load of 50N. 70N and 90N under room temperature. The transformation of phase and the value of residual stress were measured by X-ray diffraction method(XRD). Tribological characteristics and wear mechanisms of coatings was observed by SEM. The tribological wear performance was discussed a point of view for residual stress. Consequently, post-spray heat treatment plays an important role in decreasing residual stress. Residual stress in coating system has a significant influence on the wear mechanism of coating.

수술실 영역에서의 임상의공(CE)업무에 대한 고찰

채영한,전석봉,김영서,천승민,박현상,권혁남 大韓醫用生體工學會 2000 대한의용생체공학춘계학술대회논문집 Vol.22 No.1

CE(Clinical Engineering) fields have been emphasized on simultaneous assistance while clinical operation including normal maintenance. Especially in OR (Operating Room), composite CE forms are requested from its importance and emergent situations. We have carried out CE jobs for seven years with classifying it into three major parts, BO(Before Operation), OO(On operation), and AO(After Operation). Based on these experiences, we studied on more efficient and pertinent CE jobs to our domestic situation by analyzing and comparing with foreign's and are going to suggest just a model of standard formations of CE in this paper.

한국천문연구원 Hα 태양전면관측시스템의 바닥고르기 연구

박형민,김연한,봉수찬,박영득,조경석,장비호,최성환,나자경,이승민,채종철,Park, H.M.,Kim, Y.H.,Bong, S.C.,Park, Y.D.,Cho, K.S.,Jang, B.H.,Choi, S.,Nah, J.,Lee, S.M.,Chae, J. 한국천문학회 2008 天文學論叢 Vol.23 No.2

We have performed the flat-fielding correction for the $H{\alpha}$ full-disk monitoring system of KASI (Korea Astronomy and Space Science Institute), which is installed in the Solar Flare Telescope (SOFT) at the top of Bohyun Mountain. For this, we used a new method developed by Chae (2004), to determine the flat pattern from a set of relatively shifted images. Using this method, we successfully obtained the flat pattern for $H{\alpha}$ full-disk observations and compared our result with the image observed in Catania Astrophysical Observatory. The method that we used in this study seems to be quite powerful to obtain the flat image for solar observations. In near future, we will apply this method for the flat-fielding correction of all solar imaging instruments in KASI.

이동전화를 이용한 E.C.G DATA 전송

박현상,전석봉,김영서,천승민,채영한,권혁남 大韓醫用生體工學會 1999 대한의용생체공학춘계학술대회논문집 Vol.21 No.1

In the case of emergency room, which including the patient transfort procedure. there are some difficulties caused by the absence of former information about the patient. This information offers basic environment to doctor's behavior and makes doctor ready for the further stpes have to be done. So we tried to improve this point by offering the data of patient's E.C.G way e. just through the reconstructions of current equipment and wireless transfer system.

蛋白資源으로서의 大豆 增産 및 利用性 向上에 관한 硏究

李弘석,潘性紈,柳順昊,蔡永岩,林善旭,李炯周,韓仁圭 서울大學校農科大學 1984 서울대농학연구지 Vol.9 No.1

1. 大豆의 需給에 관한 硏究. (1) 大豆는 直接 食用消費와 飼料로서 間接消費가 급격히 增加 하고 있다. (2) 1968∼1981년 사이에 大豆의 用途別 消費構造가 크게 變化되어 食用消費에서 飼 料用 消費로 移動하고 있는 것이 뚜렷하다. (3) 大豆 消費量을 推定한 결과 總消費量은 1981年의 ??에서 1993년에는 ??으로 약 2.4배가 될것으로 예상된다. (4) 消費를 충족시키기 위해서는 막대한 양의 輸入이 불가피하지만 國內生産의 增大 와 質的 向上에 관한 方案이 시급히 모색되어야 한다. 2. 良質高蛋白 多收性品種의 選拔 및 育成 (1) 良質高蛋白 多收性品種의 育成을 위하여 母本을 선정하고 人工交配를 實施하여 58組合 670粒의 交配種子를 얻었다. (2) 雜種世代로부터 41組合의 247 F₂個體, 32組合 203系統의 F₃系統, 10組合의 25F₄系統을 선발하였다. (3) 生産力 檢定豫備試驗에서 3系統이 그리고 本試驗에서 2系統이 극히 多收性으로 선발되어 有望視되며 다음 試驗에 供試될 豫定이다. 3. 耕種方法에 따른 大豆蛋白의 量的 및 質的 向上에 관한 硏究. (1) 大豆의 量的收量增加를 위해서는 適正量의 施肥가 要求된다. (2) 전반적으로 볼때 소석회나 요소 엽면 시비는 등숙, 분지수, 株當협수, 株당粒 數, 100粒重 및 收量에 영향을 주고 있지만 標準肥栽培로 충분한 것으로 판단된 다. (4) 含黃 아미노산인 methinie과 cystine의 함량증가에 유황은 效果가 없었다. 4. 大豆根瘤菌의 分離 및 選別 (1) 募集 分離된 菌은 培養的 特性 및 生理試驗에 의하여 大豆根瘤菌種임을 확인하 였다. (2) 大豆品種 東北太, 長葉콩 및 Wayne에서 分離한 것이 根瘤의 差生 및 colony 發 育相이 우수하였다. 5. 大豆의 品種과 施肥에 따른 蛋白質 및 아미노산의 造成變化 (1) 品種間 蛋白質 含量에 큰 차이를 나타내고 있었다. (2) 品種內에서 시비에 따른 蛋白質 含量에 차이가 없었다. (3) 含硫黃 아미노산의 함량이 蛋白質 100g당 5.1∼6.4g으로 상당히 높았으나 품종 간이나 시비 방법에 따른 차이는 크지 않았다. 6. 國內産 大豆의 Broiler에 대한 飼料的 價値에 關하나 硏究 (1) 증체량, 사료섭취량 및 사료효율은 공시품종간에 유의적인 차이를 나타내지 않 았으나 대두 품종 봉의의 증체량이 제일 높았다. (2) 공시 대두는 어분이나 casein에 비하여 높은 증체율을 보았다. (3) 공시품종간에 AME와 TME에는 유의적인 차이를 보이지 않았다. Soybean has been cultivated as main protein source for a long time and it is also an important oil crop. As our living standard is getting better, higher quality in soybean protein and larger quantity of soybean are demanded. Therefore this study was conducted to provide comprehensive understanding for better utilization and processing of soybean as well as improvement of soybean breeding lines for higher yield with better guality. The results are summarized as follows: 1. Study on the demand and supply relationslip of soybean. (1) Demand for soybean as direct daily use of food and feed grain has been rapidly increased for past 13 years. (2) Utilization pattern of soybean had been shifted from direct use of food to feed grain during 1968∼1981. (3) Consumption of soybean will be increased 2.4 fold from ?? in 1981 to ?? in 1993. (4) Huge amount of soyben sbould be imported to satisfy the demand. Therefore certain measures soybean production in land and improvement of soybean protein guality should be considered. 2. Study on the selection and breeding for high yielding varieties with high protein content and better quality of protein in soybean. (1) For the breeding of high yield and high protein content with better quality in soybean, artificial cross between selected parents were made and obtained 670 crossed seeds in 58 combinations. (2) Two hundred forty seven F₂plants of 41 combinations, 203 F₃lines of 32 combinations and 25 F₄lines of 10 combinations were selected from the hybrid population for next generation, respectivly. (3) The results of preliminary yield trial and yield trial, three ?? lines and two ?? lines were selected as the promising lines. These lines will be tested for further yield test and put in regional adaptive trials. 3. Study on the improvement of quantity and quality of soybean protein. (1) Proper fertilization levels must be studied for increase of soybean yield. (2) Although lime and leaf spray of urea influened on the maturity, branch numbers, pod numbers per plant and grain numbers per plant in general, however, these had no positive effects on yield increase under fertrlizer levels used in this experiment. (3) Sulfur from ammonium sulfate fertilizer was ineffective for increase sulfur containing amino acids such as methionine and cystine. 4. Isolation and selection of Rhizobium strains collectecd in soybean fields. (1) Selected strains were confirmed as soybean nodulation bacteria by their cultural characteristics and by physiological test. (2) Strains isolated from cv. Dongbuktae, Jangyeobkong and Wayne were good in nodulation and colony formation. 5. Study on the variation of protein and amino acid composition of protein due to varietal differences and fertilizations. (1) Protein content was varied with varieties. (2) Fertilizations used in this experiment did not affect on protein content. (3) Contents of sulfur bearing amino acids were relatively high in varieties used and were 5.1∼6.4 grams of amino acid per 100 grams of protein, but the differences among the varieties and different fertilizations were not significant. 6. Studies on the nutiritive values of locally produced soybean for the broiler chicken. (1) Although the weight gain of chicks, feed intake and feed efficiency wre not statistically different among three varieties used, however, weight gain of chicks fed with cv. Bongyi was the highest. (2) Chicken fed with soybean grew better than those fed with fish meal or casein. (3) There was no significant difference among soybean varieties used in apparent metabolizable energy and true metabolizable energy.

카메라를 이용한 흔들림 계측 및 제어

김영복(Y. B. Kim),H. Kawai,최용운(Y. W. Choi),이권순(K. S. Lee),채규훈(G. H. Chae) 한국동력기계공학회 2007 한국동력기계공학회 학술대회 논문집 Vol.- No.-

In general, the swing motion of the crane is controlled and suppressed by activating the trolley motion. In many papers reported by us, we suggested a new type of anti-sway control system of the crane. In the proposed control system, a small auxiliary mass(moving-mass) is installed on the spreader and the swing motion is controlled by moving the auxiliary mass. The actuator reaction against the auxiliary mass applies inertial control forces to the container in order to reduce the swing motion in the desired manner. The measuring system is based on laser sensor or others. However it is not so useful in real world. In this paper, we use the camera based measurement system to measure sway motion of the crane. And the H∞ based control technique is applied to suppress swing motion of the crane. And the experimental result shows that the proposed control system is useful and robust to disturbances.