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RISS 인기검색어
- 검색키워드
- 저자 : Xuexia Zhang (검색결과 3 건)
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Identification and phylogeny of five male-specific lethal genes in the silkworm Bombyx mori
Wenbin LIU,Yong ZHANG,Xuexia MIAO,Yongping Huang 한국곤충학회 2008 Entomological Research Vol.38 No.-
In the fruit fly (Drosophila melanogaster) dosage compensation equalization of X-linked genes between the sexes is achieved via a complex that is termed the compensasome, which is composed of at least five male-specific lethal (msl) genes and two non-coding RNAs. In the present study, we cloned all five homologs (termed the msls) from the silkworm, Bombyx mori, in which the issue of dosage compensation remains controversial. Data mining and robust phylogenetic analysis revealed that msls are ubiquitously present in genomes, from insects to mammals, with unknown biological functions. However, the five genes seem to have evolved at different times due to gene duplication. Real-time reverse transcriptase-polymerase chain reaction experiments demonstrated that in the silkworm, some Z-linked genes are expressed at higher levels in females than in males while others are expressed at lower levels in females than in males, which suggests that the msl genes in the silkworm and fruit fly may have different roles. We discussed the relationship of phylogeny between msl and sex-lethal.
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Xiao Kai,Liu Xiaohui,Zhang Aidong,Zha Dingshi,Zhu WeiMin,Tan Feng,Huang Qianru,Zhou Yaru,Zhang Min,Li Jianyong,Wu Xuexia 한국원예학회 2022 Horticulture, Environment, and Biotechnology Vol.63 No.5
Browning of fresh-cut eggplant (Solanum melongena L.) reduces its sensory and nutritional qualities and further influences consumption. Polyphenolic oxidases (PPOs) are key enzymes involved in browning, but the mechanisms that regulate the expression of PPO genes are still unclear. Here, 12 SmPPO genes were identified and phylogenetic analysis clustered these genes into four branches. Protein and cis-regulatory element analyses showed that the SmPPO gene family has a conserved gene structure and diverse functions. Gene expression analysis in different tissues showed that the expression of SmPPO2, SmPPO3, SmPPO6, SmPPO7, and SmPPO10 was higher in the flesh of the browning-sensitive inbred line ‘36’ than in the flesh of the browning-resistant line ‘Fu’. Furthermore, almost all SmPPO genes in ‘36’ were upregulated at 4 °C and 36 °C compared with those in ‘Fu’, and the expression increased earlier after harvest. In addition, SmPPO1, SmPPO6, SmPPO7, and SmPPO10 expression was significantly elevated in ‘36’ after 2 days at 36 °C. These results suggest that SmPPOs are key modulators of eggplant browning and provide candidate genes for further research on the mechanisms regulating fruit browning.
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Yi Li,Qing'an Zeng,Jiliang Qiu,Ting Pang,Fenglian Ye,Lin Huang,Xuexia Zhang 한국유방암학회 2020 Journal of breast cancer Vol.23 No.4
Purpose: Four and a half LIM protein 1 (FHL1) is involved in breast cancer (BC) development, but the regulatory mechanism involved remain unclear. In the present study, we examined the role of FHL1 in BC development. Methods: The expression of FHL1, miR-183-5p, and miR-96-5p in BC tissues was analyzed using StarBase analysis. FHL1 expression in BC tissues, a normal human breast epithelial cell line, and BC cell lines was detected using quantitative reverse transcription polymerase chain reaction (qRT-PCR). The relationship between FHL1 and miR-183-5p/miR-96-5p was analyzed via Pearson's rank correlation, TargetScan, and a dual-luciferase reporter assay. BT549 and MDA-MB-231 cells were transfected with either FHL1 and miR-183-5p mimics, or siFHL1 and a miR-183-5p inhibitor, respectively. The viability, colony number, migration, invasion, and tube length of BT549 and MDA-MB-231 cells were examined using cell counting kit-8, colony formation, wound-healing, Transwell, and tube formation assays, respectively. The levels of FHL1, vascular endothelial growth factor (VEGF), p53, E-cadherin, N-cadherin, and vimentin were quantified using western blotting and qRT-PCR. Results: FHL1 expression was downregulated in BC tissues and cells, whereas miR-183-5p and miR-96-5p were upregulated in BC tissues (negative correlation with FHL1 expression). FHL1 overexpression inhibited the viability, colony number, migration, and invasion of BC cells and the expression of VEGF, N-cadherin, and vimentin, and increased the expression of FHL1, p53, and E-cadherin in BT549 cells. Furthermore, a miR-183-5p mimic reversed these effects of FHL1 overexpression, whereas FHL1 silencing caused opposite results to those observed in MDA-MB-231 cells; however, this was reversed by a miR-183-5p inhibitor. Conclusion: Our study suggests that miR-183-5p promotes cell proliferation, metastasis, and angiogenesis by negatively regulating FHL1 in BC.
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