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Lipase-catalyzed Production of Solid Fat Containing Conjugated Linoleic Acid in Binary Models
Xue-Mei Zhu,Md. Abdul Alim,Jiang-Ning Hu,Prakash Adhikari,Jeung-Hee Lee,Ki-Teak Lee 한국식품과학회 2009 Food Science and Biotechnology Vol.18 No.3
Solid fats were esterified with solid phase of rice bran oil (S-RBO), palm stearin (PS), and conjugated linoleic acid (CLA) at 2 substrate mole ratios (S-RBO:PS:CLA of 1:1:2 and 1:3:4). The major fatty acids were palmitic, oleic, and CLA in 36 hr products. The solid fat content (SFC) of the 1:1:2 product was 12.8% while the SFC of 1:3:4 product was 45.1% at 20℃. The SFCs after 20℃ reduced when the reaction time increased from 1 to 36 hr, suggesting that the change of triacylglycerol species was augmented by extending reaction time.
Zhu, Xue-Mei,Hu, Jiang-Ning,Shin, Jung-Ah,Lee, Jeung-Hee,Hong, Soon-Teak,Lee, Ki-Teak The Korean Society of Food Science and Nutrition 2010 Preventive Nutrition and Food Science Vol.15 No.4
The chemical characteristics of seed oils of Asian ginseng (Panax ginseng C.A. Meyer) at different ages grown in Korea (3, 4 and 5-year old) and China (5-year old), and American ginseng (Panax quinquefoliu L., 5-year old) grown in China were compared. Total fatty acid composition showed a significantly higher oleic acid content in American (87.50%) than in Korean (68.02~69.14%) and Chinese ginseng seed oils (61.19%). At the sn-2 position, the highest oleic acid (81.09%) and lowest linoleic acid (15.77%) were found in American ginseng seed oil. The main triacylglycerol species in ginseng seed oils were triolein (OOO) and 1,2-dioleoyl-3-linoleoyl-glycerol (LOO)/1,3-dioleoyl-2-linoleoyl-glycerol (OLO). In addition, the seed oils possessed an ideal oxidative stability showing 16.55~23.12 hr of induction time by Rancimat test. The results revealed that ginseng seed oil could be developed as a new healthy edible oil, and that the oil's chemical characteristics were strongly associated with the ginseng species and habitats.
Xue-Mei Zhu,Jiang-Ning Hu,Jung-Ah Shin,Jeung-Hee Lee,Soon-Teak Hong,Ki-Teak Lee 한국식품영양과학회 2010 Preventive Nutrition and Food Science Vol.15 No.4
The chemical characteristics of seed oils of Asian ginseng (Panax ginseng C.A. Meyer) at different ages grown in Korea (3, 4 and 5-year old) and China (5-year old), and American ginseng (Panax quinquefoliu L., 5-year old) grown in China were compared. Total fatty acid composition showed a significantly higher oleic acid content in American (87.50%) than in Korean (68.02~69.14%) and Chinese ginseng seed oils (61.19%). At the sn-2 position, the highest oleic acid (81.09%) and lowest linoleic acid (15.77%) were found in American ginseng seed oil. The main triacylglycerol species in ginseng seed oils were triolein (OOO) and 1,2-dioleoyl-3-linoleoyl-glycerol (LOO)/1,3-dioleoyl-2-linoleoyl-glycerol (OLO). In addition, the seed oils possessed an ideal oxidative stability showing 16.55~23.12 hr of induction time by Rancimat test. The results revealed that ginseng seed oil could be developed as a new healthy edible oil, and that the oil’s chemical characteristics were strongly associated with the ginseng species and habitats.
Xue Zhu,Ke Wang,Fanfan Zhou,Ling Zhu 대한약학회 2018 Archives of Pharmacal Research Vol.41 No.10
Abnormal accumulation of the free-form alltrans- retinal (atRAL), a major intermediate of human visual cycle, is considered to be a key cause of retinal pigment epithelial (RPE) dysfunction in the pathogenesis of retinal degenerative diseases such as age-related macular degeneration (AMD). Paeoniflorin (PF), a monoterpene glucoside isolated from Paeonia lactiflora Pall., has been used in clinical treatment of retinal degenerative diseases in China for several years; however, the underlying mechanism remains unclear. The aim of this study is to investigate the protective effect of PF against atRAL toxicity in human ARPE-19 cells and its molecular mechanism. The results of our study showed that the pre-treatment of PF dose-dependently attenuated atRAL-induced cell injury by the reduction of Nox1/ROS-associated oxidative stress, mitochondrial dysfunction and GRP78-PERK-eIF2a- ATF4-CHOP-regulated endoplasmic reticulum (ER) stress in ARPE-19 cells. Additionally, our data showed that PF mainly exerted its activity via triggering calcium-calmodulin dependent protein kinase II (CaMKII)-mediated activation of AMP-activated protein kinase (AMPK). AMPK inhibition significantly reversed the protective effect of PF against atRAL toxicity in ARPE-19 cells. Overall, our findings provided the novel mechanism of PF protecting human RPE cells, which may prevent the progression of retinal degenerative diseases.
Xue-Mei Zhu,Jiang-Ning Hu,이정희,Yang Dan,이기택 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.5
The oxidative stabilities of enzymatic modifiedlipids (EMLs) were studied using pine nut oil (PN) andpalm stearin (PS) as substrates (w/w, 40:60; 30:70) vs. aphysical blend (PB). The amount of pinolenic acid (PLA)at the sn-2 position in EML was increased to 5.5%compared to 0.8% in PB. The acid, peroxide, p-anisidine,and TBARS values were used to measure the oxidativestability of EML and PB. After 30 days of oxidation, only1.5 and 0.7% of total PLA at sn-2 position PLA weredetected in EML of PN:PS 40:60 and PN:PS 40:60,respectively. EMLs were more vulnerable to oxidizationthan PB. Different concentrations of rosemary (1,000 and2,000 mg/kg) and catetchin (400 and 800 mg/kg) wereadded to EMLs to reduce the oxidation level. Resultsindicated that these two antioxidants deterred the extent ofoxidation in EMLs.