A New 5α,8α-Epidioxy Sterol from a Marine Sponge Psammocinia Species

Hye Jin Park1,,Xuan Luo1,,Jongki Hong2,,Dong-kyoo Kim3,,Kwang Sik Im1, and,Jee H. Jung1* 한국생약학회 2005 Natural Product Sciences Vol.11 No.4

An investigation of the MeOH soluble fractions of a marine sponge Psammocinia sp. (Order:Dictyoceratida) led to the isolation of a new epidioxy sterol (1) and four known sterols (2-5). Their planarstructures were defined by analyses of the spectroscopic data. The 27-nor-24-methylcholestan type side chain withan epidioxy nucleus (1) was unprecedented. Compounds 1-5 were isolated from a sponge Psammocinia sp. for the first time.

Autophagy Inhibition Promotes Gambogic Acid-induced Suppression of Growth and Apoptosis in Glioblastoma Cells

Luo, Guo-Xuan,Cai, Jun,Lin, Jing-Zhi,Luo, Wei-Shi,Luo, Heng-Shan,Jiang, Yu-Yang,Zhang, Yong Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12

Objective: To investigate the effects of gambogic acid (GA) on the growth of human malignant glioma cells. Methods: U251MG and U87MG human glioma cell lines were treated with GA and growth and proliferation were investigated by MTT and colony formation assays. Cell apoptosis was analyzed by annexin V FITC/PI flow cytometry, mitochondrial membrane potential assays and DAPI nuclear staining. Monodansylcadaverine (MDC) staining and GFP-LC3 localisation were used to detect autophagy. Western blotting was used to investigate the molecular changes that occurred in the course of GA treatment. Results: GA treatment significantly suppressed cell proliferation and colony formation, induced apoptosis in U251 and U87MG glioblastoma cells in a time- and dose-dependent manner. GA treatment also lead to the accumulation of monodansylcadaverine (MDC) in autophagic vacuoles, upregulated expressions of Atg5, Beclin 1 and LC3-II, and the increase of punctate fluorescent signals in glioblastoma cells pre-transfected with GFP-tagged LC3 plasmid. After the combination treatment of autophagy inhitors and GA, GA mediated growth inhibition and apoptotic cell death was further potentiated. Conclusion: Our results suggested that autophagic responses play roles as a self-protective mechanism in GA-treated glioblastoma cells, and autophagy inhibition could be a novel adjunctive strategy for enhancing chemotherapeutic effect of GA as an anti-malignant glioma agent.

A Study on the Customer Experience Design through analyzing Smart Hotels in China

Luo Xuan,Yonghwan Pan 한국융합학회 2021 한국융합학회논문지 Vol.12 No.3

코로나(COVID-19) 폭발로 인해 비접촉 서비스의 특징과 장점이 갈수록 두드러졌고 스마트호텔의 발전이 가속 화되었다. 본 연구는 서로 다른 접촉점에서고객 체험 과정에 발생하는 스마트 서비스 체험을 식별, 분류 및 정의하는 데에 목적을 두고 있다. 기존 연구와 문헌을 바탕으로 스마트 호텔의 개념과 특징에 대해 연구 토론해 보았다. 스마트 호텔의 스마트 체험 인자와 고객 접촉점을 활용하여 분석 프레임 워크를 디자인하였다. 이로써 중국에서 선별된 스마트 호텔에 대해 현장 조사 및 분석을 수행한다. 사례 분석 결과를 보면 스마트 호텔의 고객 체험 디자인이 교호성, 개별화, 접근 가능성, 정보성, 프라이버시 안전성에 어느 정도 발전하고 있다는 것을 알 수가 있다. 위의 결과를 바탕으로 본 논문에서는 미래의 스마트 호텔 CX 디자인이 개인화된 서비스 관련 체험을 개발하기 위해 데이터를 통합하는 방향으 로 발전하는것을 제안한다. The outbreak of covid-19 has brought the characteristics and advantages of non-contact services to increased prominence, and the development of smart hotels”has accelerated. This study aims to identify, categorize and define the smart service experience at different touch points of the customer experience. The concept and characteristics of the smart hotel were examined based on existing research and literature. An analytical framework was designed using smart experience factors and customer touch points of smart hotels. Selected Chinese smart hotels were then examined under this framework. The case analysis results show that the customer experience design of smart hotels has developed to different degrees, in terms of interactivity, personalization, accessibility, information and privacy security. Based on the above findings, this article suggests that the design of smart hotels should use integrated data to further enhance personalized service experience.

도시 부티크 호텔의 서비스디자인에 관한 연구 : 중산층 소비자의 경험을 중심으로

Luo Xuan,Pan Young Hwan 한국서비스디자인학회 2019 한국서비스디자인학회 학술대회자료집 Vol.2019 No.1

Development of a Microclimate Model for the Environmental Control Strategy Decision in a Desktop Growth Chamber

( Shao-xuan Luo ),( Chia-chyi Wu ),( Ming-yih Chang ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1

The plant factory was a rapid-developed agricultural technology in East Asia in the past decade. It provides a well-controlled environment for crop growth to guarantee better yield or quality. However, its high energy consumption always leads to criticism. A good environmental control strategies would achieve better plant growth environment or efficient energy utilization. Unfortunately, the control strategy was usually based on the previous growth experience in the field. It is necessary to develop a fast and low-cost, easy-replicated approach to verify the performance of the control strategies. The objective of this study was to develop a microclimate model to simulate the environment conditions when using different environmental control strategies. This model was based on temperature-humidity equilibrium and plant evapotranspiration equations. The ambient environmental conditions, parameters of control strategy, plant evapotranspiration rate were input into this model and the outputs were the calculated temperature and humidity. A desktop grow chamber was used to verify the feasibility of the microclimate model. Ten plants of hydroponically grown lettuce were grown in this chamber. The results shows that the simulation results of this model fit the real cultivation results. This model provided an easy and low cost way to screen the feasible control strategies by simulated the environment change in a plant factory.

중국 스마트 호텔을 위한 상호 작용 디자인 관점의 서비스 디자인

루쉬안 ( Luo Xuan ),반영환 ( Pan Young Hwan ) 한국기초조형학회 2019 한국기초조형학회 학술발표논문집 Vol.2019 No.1

Information Security in the Age of Algorithms

Fei Yang(Fei Yang),Xuan Luo(Xuan Luo) 아시아사회과학학회 2023 Jornal of Asia Social Science Vol.10 No.1

Algorithms are now widely used in all areas of social life, building a new social picture and reality. As a technology based on big data modelling and analysis, algorithms are embedded in all aspects of information production and distribution by means of technology, and become an important force in the construction of information environment. Under the algorithmic logic of information dissemination, the visible and invisible are in fact influenced by the algorithm as an authoritative mediator and mediator. From Daniel Dayan's 'visibility' perspective, this paper examines the risks posed by algorithms in three areas: the risk of passive disclosure of personal privacy, the use and control of personal data by profit-seeking algorithms, and the polarisation of information cocoons and circles. The information security risk brought by algorithms is attempted to be tackled and improved from several aspects, such as constructing a 'trinity' information security strategy system; adhering to the mainstream value orientation and highlighting the rational balance of “tool-value” of algorithms; and bringing into play multiple mechanisms together with effects.

Developing TCM clinical practice guidelines: A comparison between traditional Chinese medicine and western medicine

Xuan Yu,Shouyuan Wu,Juanjuan Zhang,Yue Hu,Meng Luo,Hongmei Zhao,Xuping Song,Yaolong Chen,Xiaohui Wang 한국한의학연구원 2023 Integrative Medicine Research Vol.12 No.2

Clinical practice guidelines in Traditional Chinese Medicine (CPG-TCM) is the recommendation that aims to provide the best service to users by identifying and summarizing the rules of prevention, diagnosis, treatment, rehabilitation, and regression of diseases based on systematic reviews of evidence and balancing the advantages and disadvantages of different interventions for clinical questions of Traditional Chinese Medicine. Over the past 30 years, the concept and methods of evidence-based medicine have had a significant impact on the development of clinical practice guidelines in Western Medicine (CPG-WM), and their standardized guideline development methods are being adapted and used in the development of CPG-TCM. However, the quality of CPG-TCM is far from CPG-WM, and the methodological system for developing CPG-TCM is not yet fully established. Therefore, this study aims to explore the methodological differences between CPG-TCM and CPG-WM and to inform the development of high-quality CPGTCM.

Biotechnology : Microbial Desulfurization of Dibenzothiophene and 4,6-Dimethyldibenzothiophene in Dodecane and Straight-Run Diesel Oil

( Luo Ming Fang ),( Xing Jian Min ),( Gou Zhong Xuan ),( Liu Hui Zhou ),( Chen Jia Yong ) 한국화학공학회 2003 Korean Journal of Chemical Engineering Vol.20 No.4

The desulfurization of dibenzothiophene (DBT), 4,6-dimethyldibenzothiophene (4,6-DMDBT) and their mixture by lyophilized cells of Pseudomonas delafieldii R-8 was studied in the presence of dodecane. The desulfurization rate for 4,6-DMDBT was found to be about 40% in comparison with that for DBT. The desulfurization process for DBT and 4,6-DMDBT proceeded simultaneously without preference for either one. The desulfurization rate for each compound was decreased when they were mixed together. The extent of desulfurization of 4,6-DMDBT was increased with the increase of cell concentration and the decrease of the volume ratio of oil-to-water used. The specific desulfurization rate for 4,6-DMDBT could be reached to 10.4 mmol sulfur kg^(-1) (cell) h^(-1) [approximately 0.33 mg sulfur g^(-1) (cell) h^(-1)]. Pseudomonas delafieldii R-8 showed high desulfurization capability for straight-run diesel oil (containing 1,807 mg/L of sulfur). About 1,000 mg/L of sulfur in diesel oil was removed by resting cells of this strai in 24 h of reaction. The specific desulfurization rate was 8.75 mmol sulfur kg^(-1) (cell) h^(-1).

Lactate exacerbates lung damage induced by nanomicroplastic through the gut microbiota–HIF1a/PTBP1 pathway

Xuan Lihui,Xu Zheng,Luo Jinhua,Yin Wang,Yan Yuhui,Qu Can,Xie Zuozhong,Skonieczna Magdalena,Zhou Ping-Kun,Huang Ruixue 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Exposure to nanomicroplastics (nano-MPs) can induce lung damage. The gut microbiota is a critical modulator of the gut–lung axis. However, the mechanisms underlying these interactions have not been elucidated. This study explored the role of lactate, a key metabolite of the microbiota, in the development of lung damage induced by nano-MPs (LDMP). After 28 days of exposure to nano-MPs (50–100 nm), mice mainly exhibited damage to the lungs and intestinal mucosa and dysbiosis of the gut microbiota. Lactate accumulation was observed in the lungs, intestines and serum and was strongly associated with the imbalance in lactic acid bacteria in the gut. Furthermore, no lactate accumulation was observed in germ-free mice, while the depletion of the gut microbiota using a cocktail of antibiotics produced similar results, suggesting that lactate accumulation in the lungs may have been due to changes in the gut microbiota components. Mechanistically, elevated lactate triggers activation of the HIF1a/PTBP1 pathway, exacerbating nano-MP-induced lung damage through modulation of the epithelial–mesenchymal transition (EMT). Conversely, mice with conditional knockout of Ptbp1 in the lungs (Ptbp1flfl) and PTBP1-knockout (PTBP1-KO) human bronchial epithelial (HBE) cells showed reversal of the effects of lactate through modulation of the HIF1a/PTBP1 signaling pathway. These findings indicate that lactate is a potential target for preventing and treating LDMP.