RNA m5C modification upregulates E2F1 expression in a manner dependent on YBX1 phase separation and promotes tumor progression in ovarian cancer

Liu Xiaoyi,Wei Qinglv,Yang Chenyue,Zhao Hongyan,Xu Jie,Mobet Youchaou,Luo Qingya,Yang Dan,Zuo Xinzhao,Chen Ningxuan,Yang Yu,Li Li,Wang Wei,Yu Jianhua,Xu Jing,Liu Tao,Yi Ping 생화학분자생물학회 2024 Experimental and molecular medicine Vol.56 No.-

5-Methylcytosine (m5C) is a common RNA modification that modulates gene expression at the posttranscriptional level, but the crosstalk between m5C RNA modification and biomolecule condensation, as well as transcription factor-mediated transcriptional regulation, in ovarian cancer, is poorly understood. In this study, we revealed that the RNA methyltransferase NSUN2 facilitates mRNA m5C modification and forms a positive feedback regulatory loop with the transcription factor E2F1 in ovarian cancer. Specifically, NSUN2 promotes m5C modification of E2F1 mRNA and increases its stability, and E2F1 binds to the NSUN2 promoter, subsequently reciprocally activating NSUN2 transcription. The RNA binding protein YBX1 functions as the m5C reader and is involved in NSUN2-mediated E2F1 regulation. m5C modification promotes YBX1 phase separation, which upregulates E2F1 expression. In ovarian cancer, NSUN2 and YBX1 are amplified and upregulated, and higher expression of NSUN2 and YBX1 predicts a worse prognosis for ovarian cancer patients. Moreover, E2F1 transcriptionally regulates the expression of the oncogenes MYBL2 and RAD54L, driving ovarian cancer progression. Thus, our study delineates a NSUN2-E2F1-NSUN2 loop regulated by m5C modification in a manner dependent on YBX1 phase separation, and this previously unidentified pathway could be a promising target for ovarian cancer treatment.

High efficient adsorption of gold ions onto the novel functional composite silica microspheres encapsulated by organophosphonated polystyrene

Ping Yin,Rongjun Qu,Mingyu Xu,Wei Liu,Xiguang Liu,Qiang Xu 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.2

The novel functional composite silica microspheres encapsulated by organophosphonated polystyrene(SG–PS–N–P) has been successfully synthesized. SG–PS–N–P was employed to adsorb Au(III) fromsimulated wastewater, and it exhibited excellent performance, and the maximum adsorption capacitywas 980.39 mg/g at 35 ℃. The adsorption process optimization was performed using response surfacemethodology (RSM), and the analysis of variance (ANOVA) of the quadratic model demonstrated that themodel was highly significant. Moreover, the regeneration capacities of SG–PS–N–P were investigated,and it has been found that the adsorption capability remains high after several cycles of adsorption–desorption.

Neutropenia during the First Cycle of Induction Chemotherapy Is Prognostic for Poor Survival in Locoregionally Advanced Nasopharyngeal Carcinoma: A Real-World Study in an Endemic Area

Cheng Xu,Shi-Ping Yang,Yuan Zhang,Ling-Long Tang,Guan-Qun Zhou,Xu Liu,Yan-Ping Mao,Rui Guo,Wen-Fei Li,Lei Chen,Ai-Hua Lin,Ying Sun,Jun Ma 대한암학회 2018 Cancer Research and Treatment Vol.50 No.3

Purpose The purpose of this study was to investigate the effect of neutropenia during the first cycle of induction chemotherapy (IC-1) on survival in locoregionally advanced nasopharyngeal carcinoma (LANPC). Materials and Methods Eligible patients (n=545) with LANPC receiving IC+concurrent chemoradiotherapy were included. Based on nadir neutrophil after IC-1, all patients were categorized into three groups: no/grade 1-2/grade 3-4 neutropenia. Five-year overall survival (OS) and disease-free survival (DFS) were compared between groups and subgroups stratified by IC regimen. We also explored the occurrence of IC-1–induced myelosuppression events and the minimal value of post-treatment neutrophil-to-lymphocyte ratio (post-NLRmin). Univariate/multivariate analyses were performed to investigate the effect of IC-1–induced neutropenia, timing of neutropenia, number of myelosuppression events, and high post-NLRmin on OS/DFS. Results Grade 1-2/grade 3-4 neutropenia were associated with poorer OS/DFS than no neutropenia (all p < 0.05); OS/DFS were not significantly different between patients experiencing grade 1-2 vs. 3-4 neutropenia. Neutropenia had no significant effect on OS/DFS in patients receiving docetaxel–cisplatin–5-fluorouracil (TPF). Grade 1-2 (grade 3-4) neutropenia negatively influenced OS/DFS in patients receiving cisplatin–5-fluorouracil (PF) (PF and docetaxel– cisplatin [TP]; all p < 0.05). Neutropenia, two/three myelosuppression events, and high post-NLRmin ( 1.33) was most frequent on days 5-10, second and third week of IC-1, respectively. After adjustment for covariates, IC-1–induced neutropenia, two/three myelosuppression events, and post-NLRmin  1.33 were validated as negative predictors of OS/DFS (all p < 0.05); timing of neutropenia had no significant effect. Conclusion Occurrence of neutropenia, number of myelosuppression events, and high post-NLRmin during PF/TP IC-1 have prognostic value for poor survival in LANPC.

Antibiotic resistance, biochemical typing, and PFGE typing of Bifidobacterium strains commonly used in probiotic health foods

Xu, Feili,Wang, Junping,Guo, Yunchang,Fu, Ping,Zeng, Huawei,Li, Zhigang,Pei, Xiaoyan,Liu, Xiumei,Wang, Shuo 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.2

This study firstly analyzed the antibiotic resistance, biochemical typing, and pulsed-field gel electrophoresis typing of 45 Bifidobacterium strains commonly used in health foods. Most strains were resistant to antibiotics but their antibiotic resistance rates were not high: Fos (56.52%), TET (43.48%), CRO (21.74%), AMC (15.22%), GEN (13.04%), RIF (10.87%), CHL (8.7%), CTX (6.52%), VAN (4.35%), and ERY (4.35%). The 45 strains could be divided into 14 pulsed-field gel electrophoresis types, of which the strain numbers of six pulsed-field gel electrophoresis types were more than one. All the Bifidobacterium strains could be divided into nine types by API50CHL biochemical identification. The same species displayed same biochemical typings, expect for B. animalis. Furthermore, the results confirmed that the same pulsed-field gel electrophoresis-type strains had closer antibiotic resistance patterns, and the same biochemical-type strain also had similar antibiotic resistance patterns.

The Effect of Postural Correction and Subsequent Balloon Inflation in Deformity Correction of Acute Osteoporotic Vertebral Fractures

Liu, Hai-Xiao,Xu, Cong,Shang, Ping,Shen, Yue,Xu, Hua-Zi The Korean Neurosurgical Society 2014 Journal of Korean neurosurgical society Vol.55 No.6

Objective : To determine deformity correction by postural correction and subsequent balloon inflation in acute vertebral compression fractures (OVCFs) and to examine the effect of bone mineral density on deformity correction. Methods : A totol of 50 acute OVCFs received balloon kyphoplasty. Lateral radiographs were taken and analyzed at five different time points : 1) preoperative, 2) after placing the patient in prone hyperextended position, 3) after balloon inflation, 4) after deposition of the cement, and 5) postoperative. All fractures were analyzed for height restoration of anterior (Ha), middle (Hm) and posterior (Hp) vertebra as well as Cobb angle and Kyphotic angle. The bone mineral density (BMD) of lumbar spine was measured by dual-energy X-ray absorptiometry. According to the T-score, the patients were divided into two groups which were osteoporosis group and osteopenia group. Results : Postoperative measurements of Ha, Hm and the Cobb angle demonstrated significant reduction of 4.62 mm, 3.66 mm and $5.34^{\circ}$ compared with the preoperative measurements, respectively (each p<0.05). Postural correction significantly increased Ha by 5.51 mm, Hm by 4.35 mm and improved the Cobb angle by $8.32^{\circ}$ (each p<0.05). Balloon inflation did not demonstrate a significant improvement of Ha, Hm or the Cobb angle compared with baseline prone hyperextended. Postural correction led to greater improvements of Ha, Hm and Cobb angle in osteoporosis group than osteopenia group (each p<0.05). Conclusion : In acute OVCFs, the height restoration was mainly attributed to postural correction rather than deformity correction by balloon inflation. BMD affected deformity correction in the process of postural correction.

Single Purse-String Duct to Mucosa Pancreaticogastrostomy: A New Technique after Pancreaticoduodenectomy

( Xu’ An Wang ),( Ping Wang ),( Yingbin Liu ),( Shuyou Peng ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: We developed a new technique termed the single purse-string duct to mucosa Pancreaticogastrostomy, which reduced the risk of pancreatic stump bleeding caused by gastric acid corrosion and decreased the pancreatic fistula rate than Pancreaticojejunostomy. Methods: Data were collected prospectively on 75 consecutive patients (43 men and 32 women; mean age 65.3 years who underwent pancreaticoduodenectomy using the single-string duct to mucosa Pancreaticogastrostomy. The technique was performed by the same surgeon and the key point is that pancreatic stump was placed between gastric mucosa and seromuscular layer.Pancreatic stump was covered with gastric mucosa and transfixed with it beside the pancreatic duct which was inserted with a silicone tube into the stomach lumen. finally, the gastric seromuscular layer was tied with the pancreas by a purse-string suture. Results: Mean time for PG anastomosis was 19 minutes (range 17 to 32 minutes), and mean blood loss was 380 mL (range 210 to 800 mL). There was no perioperative death. Over- all morbidity rate was 34.7% (26 of 75).Two patients had PF (overall rate was 2.7%) ,No one demonstrated a grade B or C postoperative PF.Postoperative abdominal hemorrhage developed in 2 patients (2.7%), no pancreatic stump bleeding occurs. Conclusions: These results suggest that our technique is effective. In addition to lower complication rates, this technique is easy to perform and mean time of the anastomosis was 19 minutes. This kind anastomosis is a reliable, safe, and easy to perform method. From our experiences, it appears to reduce the risk for pancreatic fistula and intragastric bleeding.

Aerodynamic stability analysis of geometrically nonlinear orthotropic membrane structure with hyperbolic paraboloid in sag direction

Xu, Yun-ping,Zheng, Zhou-lian,Liu, Chang-jiang,Wu, Kui,Song, Wei-ju Techno-Press 2018 Wind and Structures, An International Journal (WAS Vol.26 No.6

This paper studies the aerodynamic stability of a tensioned, geometrically nonlinear orthotropic membrane structure with hyperbolic paraboloid in sag direction. Considering flow separation, the wind field around membrane structure is simulated as the superposition of a uniform flow and a continuous vortex layer. By the potential flow theory in fluid mechanics and the thin airfoil theory in aerodynamics, aerodynamic pressure acting on membrane surface can be determined. And based on the large amplitude theory of membrane and D'Alembert's principle, interaction governing equations of wind-structure are established. Then, under the circumstance of single-mode response, the Bubnov-Galerkin approximate method is applied to transform the complicated interaction governing equations into a system of second-order nonlinear differential equation with constant coefficients. Through judging the frequency characteristic of the system characteristic equation, the critical velocity of divergence instability is determined. Different parameter analysis shows that the orthotropy, geometrical nonlinearity and scantling of structure is significant for preventing destructive aerodynamic instability in membrane structures. Compared to the model without considering flow separation, it's basically consistent about the divergence instability regularities in the flow separation model.

Effects of Ti nonstoichiometry on microstructure, dielectric, ferroelectric and piezoelectric properties of (Na0.54Bi0.5)0.94Ba0.06Ti1+xO3 lead-free ceramics

Xu-mei Zhao,Xiao-Ming Chen,Jian Wang,Xiao-xia Liang,Jian-Ping Zhou,Peng Liu 한양대학교 세라믹연구소 2015 Journal of Ceramic Processing Research Vol.16 No.1

The lead-free ceramics (Na0.54Bi0.5)0.94Ba0.06Ti1+xO3 with x = −0.02, 0, 0.02, and 0.04 were prepared by a solid-state reaction method. Effects of Ti4+ nonstoichiometry on crystallite structure, microstructure, dielectric, ferroelectric and piezoelectric properties were studied. The ceramics with x = −0.02 and 0 exhibit typical diffraction peaks of perovskite structure. A secondary phase appears in the ceramics with x = 0.02 and 0.04. The rhombohedral-tetragonal morphotropic phase boundary exists in all samples, and the relative amount of the tetragonal phase changes with the increase in the Ti4+ amount from deficiency to excess. Grain sizes and shape of the ceramics are unaffected by changes in Ti4+ nonstoichiometry. But, dielectric, ferroelectric and piezoelectric properties of the ceramics are close related to the Ti4+ amounts. Compared with the ceramic with x = 0, the ceramics with the Ti4+ nonstoichiometry have higher depolarization temperature, lower maximum dielectric constant, decreased piezoelectric constant and ferroelectric properties.

Adjuvant Radiotherapy after Breast Conserving Treatment for Breast Cancer:A Dosimetric Comparison between Volumetric Modulated Arc Therapy and Intensity Modulated Radiotherapy

Liu, Zhe-Ming,Ge, Xiao-Lin,Chen, Jia-Yan,Wang, Pei-Pei,Zhang, Chi,Yang, Xi,Zhu, Hong-Cheng,Liu, Jia,Qin, Qin,Xu, Li-Ping,Lu, Jing,Zhan, Liang-Liang,Cheng, Hong-Yan,Sun, Xin-Chen Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.8

Background: Radiotherapy is an important treatment of choice for breast cancer patients after breast-conserving surgery, and we compare the feasibility of using dual arc volumetric modulated arc therapy (VMAT2), single arc volumetric modulated arc therapy (VMAT1) and Multi-beam Intensity Modulated Radiotherapy (M-IMRT) on patients after breast-conserving surgery. Materials and Methods: Thirty patients with breast cancer (half right-sided and half left-sided) treated by conservative lumpectomy and requiring whole breast radiotherapy with tumor bed boost were planned with three different radiotherapy techniques: 1) VMAT1; 2) VMAT2; 3) M-IMRT. The distributions for the planning target volume (PTV) and organs at risk (OARs) were compared. Dosimetries for all the techniques were compared. Results: All three techniques satisfied the dose constraint well. VMAT2 showed no obvious difference in the homogeneity index (HI) and conformity index (CI) of the PTV with respect to M-IMRT and VMAT1. VMAT2 clearly improved the treatment efficiency and can also decrease the mean dose and V5Gy of the contralateral lung. The mean dose and maximum dose of the spinal cord and contralateral breast were lower for VMAT2 than the other two techniques. The very low dose distribution (V1Gy) of the contralateral breast also showed great reduction in VMAT2 compared with the other two techniques. For the ipsilateral lung of right-sided breast cancer, the mean dose was decreased significantly in VMAT2 compared with VMAT1 and M-IMRT. The V20Gy and V30Gy of the ipsilateral lung of the left-sided breast cancer for VMAT2 showed obvious reduction compared with the other two techniques. The heart statistics of VMAT2 also decreased considerably compared to VMAT1 and M-IMRT. Conclusions: Compared to the other two techniques, the dual arc volumetric modulated arc therapy technique reduced radiation dose exposure to the organs at risk and maintained a reasonable target dose distribution.

The DNA Repair Gene ERCC6 rs1917799 Polymorphism is Associated with Gastric Cancer Risk in Chinese

Liu, Jing-Wei,He, Cai-Yun,Sun, Li-Ping,Xu, Qian,Xing, Cheng-Zhong,Yuan, Yuan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10

Objective: Excision repair cross-complementing group 6 (ERCC6) is a major component of the nucleotide excision repair pathway that plays an important role in maintaining genomic stability and integrity. Several recent studies suggested a link of ERCC6 polymorphisms with susceptibility to various cancers. However, the relation of ERCC6 polymorphism with gastric cancer (GC) risk remains elusive. In this sex- and age-matched case-control study including 402 GC cases and 804 cancer-free controls, we aimed to investigate the association between a potentially functional polymorphism (rs1917799 T>G) in the ERCC6 regulatory region and GC risk. Methods: The genotypes of rs1917799 were determined by Sequenom MassARRAY platform and the status of Helicobacter pylori infection was detected by enzyme-linked immunosorbent assay. Odd ratios (ORs) and 95% confidential interval (CI) were calculated by logistic regression analysis. Results: Compared with the common TT genotype, the ERCC6 rs1917799 GG genotype was associated with increased GC risk (adjusted OR=1.46, 95%CI: 1.03-2.08, P=0.035). When compared with (GT+TT) genotypes, the GG genotype also demonstrated a statistical association with increased GC risk (adjusted OR=1.38, 95%CI: 1.01-1.89, P=0.044). This was also observed for the male subpopulation (GG vs. TT: adjusted OR=1.71, 95%CI: 1.12-2.62, P=0.013; G allele vs. T allele: adjusted OR=1.32, 95%CI: 1.07-1.62, P=0.009). Genetic effects on increased GC risk tended to be enhanced by H. pylori infection, smoking and drinking, but their interaction effects on GC risk did not reach statistical significance. Conclusions: ERCC6 rs1917799 GG genotype might be associated with increased GC risk in Chinese, especially in males.