마그네슘 풍부 해양미네랄 용액이 hairless 마우스의 아토피성 피부염에 미치는 영향

김동희,이규재,최주봉,이영미,윤양숙,김정례,장병수,양용석 韓國電子顯微鏡學會 2008 Applied microscopy Vol.38 No.3

Atopic dermatitis (AD) is a chronically relapsing inflammatory skin disease that often has asthma and allergic rhinitis. Magnesium salts, the important component of minerals in Dead Sea water, are known to exhibit beneficial effects in inflammatory disease. Favorable effects of magnesium ions and sea water treated to the skin of patients with contact dermatitis have been reported. But histological and immunological investigations are insufficient. This study was performed to examine the inhibitory effect of magnesium-rich sea mineral water on the development of AD-like skin lesions in hairless mice. AD-like skin lesions are induced by the repeated application of 2,4-dinitrochlorobenzene (DNCB). Local application of magnesium-rich sea mineral water on hairless mice skin applied with DNCB inhibited the development of AD-like skin lesions as exemplified by a significant increase in skin hydration (p<0.01), and a decrease in epidermal water loss (p<0.01). Serum IgE level was also significantly decreased (p<0.01). These results suggest that magnesiumrich sea mineral water inhibits the development of DNCB-induced AD-like skin lesions in hairless mice. These observations indicate that magnesium-rich sea mineral water may be alternative and assistant substances for the management of AD. 아토피성 피부염은 주로 천식과 비염 등을 동반하는, 주위에서 흔히 볼 수 있는 만성 염증성 피부질환으로 유전학적, 환경적, 면역학적 요인이 복잡하게 연관되어 발병한다. 해수에 포함된 마그네슘염은 피부에 작용하여 피부장벽을 보호하는 것으로 알려지고 그에 대한 면역학적인 연구와 조직학적 연구는 아직 부족한 실정이다. 이번 연구에서는 피부염을 인위적으로 일으키는 hapten 형성물질인 DNCB를 hairless mice에 도포하여 아토피 피부염 동물 모델로 만든 후, 마그네슘이 다량 함유된 해양 미네랄수를 처리한 후 피부장벽에 미치는 영향을 관찰하였다. DNCB로 피부염을 유발한 hairless mice에 해양미네랄수를 국소적으로 도포하였을 때 유의한 피부수분함량이 증가와 경피수분손실의 감소를 확인하였다 (p<0.01). 피부측정에서 피부거칠기(skin roughness, p<0.05)와 스케일생성 (skin scaliness, p<0.01)은 실험군에서 유의한 개선효과를 나타내었으며 조직학적 검사에서도 피부손상지수의 유의한 감소 (p<0.01)와 비만세포와 (p<0.01) 호산구의 감소(p<0.05) 소견을 보였고 또한 혈청 IgE의 감소를 관찰할 수 있었다(p<0.01). 이상과 같이 마그네슘이 다량 함유된 해양 미네랄수 도포는 피부장벽의 손상을 줄이고 피부수분손실을 효과적으로 줄임으로 아토피성 피부염 증상 유발을 억제할수 있음을 확인하였다. 현재까지 아토피성 피부염의 관리를 위하여 세라마이드나 식물성 오일의 보습제가 주로 활용되고 있는 상황에서 부가적인 피부장벽의 보호를 위하여 탈염 해양 미네랄수의 활용이 가능할 것으로 판단되며 장기적으로 아토피 피부염치료의 대체, 혹은 보조적 물질로 활용될 수 있을 것으로 기대된다.

A New Full-Aperture Reflective Null Measuring Method for Conformal Dome

Xu Dong Yang,Junhua Wang,Min Xu 한국광학회 2016 Current Optics and Photonics Vol.20 No.1

In this paper, a novel full-aperture reflective null measuring method is proposed to detect the transmissionwavefront of a conformal dome surface. An aspheric compensator is designed and placed behind the dometo reflect the aspheric testing wave back to the same path. To ensure the feasibility of this method, toleranceanalysis is conducted, and guidance to assembly is given accordingly. The accuracy of this method isverified to be λ/30 (λ =3.39 μm) by Monte Carlo algorithm. In addition, the influence of different error factors,including the thickness error and decenter error of the dome, on the testing wavefront is analyzed. Simulationand experiment indicate that this method is practical and simple, and can measure the conformal domesprecisely and comprehensively.

Magnetic Properties of CoFe Films with in-plane Uniaxial Anisotropy Prepared by Electrodeposition

Yang Xu,Zhou Dong,Qiao Liang,Yang Yong,Li Fa-Shen 한국자기학회 2008 한국자기학회 학술연구발표회 논문개요집 Vol.- No.-

C-type natriuretic peptide attenuates renal osteodystrophy through inhibition of FGF-23/MAPK signaling

Dong Dong Zhang,Yang Fang Wu,Wei Xia Chen,Yao Xu,Si Yan Liu,Huang Huang Luo,Guang Mei Jiang,Yue Wu,Peng Hu 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

Renal osteodystrophy (ROD) occurs as early as chronic kidney disease (CKD) stage 2 and seems ubiquitous in almost all pediatric patients with CKD stage 5. Fibroblast growth factor (FGF)-23, a bone-derived endocrine regulator of phosphate homeostasis, is overexpressed in CKD and disturbs osteoblast differentiation and matrix mineralization. In contrast, C-type natriuretic peptide (CNP) acts as a potent positive regulator of bone growth. In the present study, we infused CNP into uremic rats and observed whether CNP could attenuate ROD through the inhibition of FGF-23 cascades. In uremic rats, CNP administration significantly alleviated renal dysfunction, calcium phosphate metabolic disorders, hypovitaminosis D, secondary hyperparathyroidism, the decrease in bone turnover markers and retarded bone pathological progression. More importantly, within FGF-23/mitogen-activated protein kinase (MAPK) signaling, the fibroblast growth factor receptor-1, Klotho and alternative (STAT-1/phospho-STAT-1) elements were upregulated by CNP, whereas FGF-23, RAF-1/phospho-RAF-1, and downstream (ERK/phospho-ERK and P38/phospho-P38) elements were paradoxically underexpressed in bone tissue. Therefore, CNP exerts a therapeutic effect on ROD through inhibition of FGF-23/MAPK signaling at the RAF-1 level.

Role of MYH Polymorphisms in Sporadic Colorectal Cancer in China: A Case-control, Population-based Study

Yang, Liu,Huang, Xin-En,Xu, Lin,Zhou, Jian-Nong,Yu, Dong-Sheng,Zhou, Xin,Li, Dong-Zheng,Guan, Xin Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

Purpose: Biallelic germline variants of the 8-hydroxyguanine (8-OG) repair gene MYH have been associated with colorectal neoplasms that display somatic $G:C{\rightarrow}T:A$ transversions. However, the effect of single germline variants has not been widely studied, prompting the present investigation of monoallelic MYH variants and susceptibility to sporadic colorectal cancer (CRC) in a Chinese population. Patients and Methods: Between January 2006 and December 2012, 400 cases of sporadic CRC and 600 age- and sex-matched normal blood donors were screened randomly for 7 potentially pathogenic germline MYH exons using genetic testing technology. Variants of heterozygosity at the MYH locus were assessed in both sporadic cancer patients and healthy controls. Univariate and multivariate analyses were performed to determine risk factors for cancer onset. Results: Five monoallelic single nucleotide polymorphisms (SNPs) were identified in the 7 exon regions of MYH, which were detected in 75 (18.75%) of 400 CRC patients as well as 42 (7%) of 600 normal controls. The region of exon 1 proved to be a linked polymorphic region for the first time, a triple linked variant including exon 1-316 $G{\rightarrow}A$, exon 1-292 $G{\rightarrow}A$ and intron 1+11 $C{\rightarrow}T$, being identified in 13 CRC patients and 2 normal blood donors. A variant of base replacement, intron 10-2 $A{\rightarrow}G$, was identified in the exon 10 region in 21 cases and 7 controls, while a similar type of variant in the exon 13 region, intron 13+12 $C{\rightarrow}T$, was identified in 8 cases and 6 controls. Not the only but a newly missense variant in the present study, p. V463E (Exon 14+74 $T{\rightarrow}A$), was identified in exon 14 in 6 patients and 1 normal control. In exon 16, nt. 1678-80 del GTT with loss of heterozygosity (LOH) was identified in 27 CRC cases and 26 controls. There was no Y165C in exon 7 or G382D in exon 14, the hot-spot variants which have been reported most frequently in Caucasian studies. After univariate analysis and multivariate analysis, the linked variant in exon 1 region (p=0.002), intron 10-2 $A{\rightarrow}G$ (p=0.004) and p. V463E (p=0.036) in the MYH gene were selected as 3 independent risk factors for CRC. Conclusions: According to these results, the linked variant in Exon 1 region, Intron 10-2 $A{\rightarrow}G$ of base replacement and p. V463E of missense variant, the 3 heterozygosity variants of MYH gene in a Chinese population, may relate to the susceptibility to sporadic CRC. Lack of the hot-spot variants of Caucasians in the present study may due to the ethnic difference in MYH gene.

Low cycle fatigue and ratcheting failure behavior of AH32 steel under uniaxial cyclic loading

Dong, Qin,Yang, Ping,Xu, Geng The Society of Naval Architects of Korea 2019 International Journal of Naval Architecture and Oc Vol.11 No.2

In this paper, the low cycle fatigue failure and ratcheting behavior, as well as their interaction of AH32 steel were experimentally investigated under uniaxial cyclic loading. The effects of mean stress, stress amplitude and stress ratio on the low cycle fatigue life and ratcheting strain were discussed. It was found that the ratcheting strain increased while the fatigue life decreased with the increase of mean stress and stress amplitude, and the increasing stress ratio would result in smaller ratcheting and larger fatigue life. Two kinds of failure modes, i.e. low cycle fatigue failure due to crack propagates and ratcheting failure due to large plastic strain will take place respectively. Based on the experimental results, considered the effect of ratcheting on fatigue life, a model with the maximum stress and ratcheting strain rate was proposed. Comparison with the experimental result showed that the new model provided a good prediction for AH32 steel.

A New Furostanol Saponin from Asparagus cochinchinensis

Yang Shen,Hai-Sheng Chen,Cong-Li Xu,Wei-Dong Xuan,Hui-Liang Li,Run-Hui Liu,Xi-Ke Xu 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.10

A new furostanol saponin, (25S)-26-O-β-D-glucopyranosyl-5β-furost-20(22)-en-3β, 15β,26-triol-3-O-[α-L-rhamnopyranosyl-(1-4)]-β-D-glucopyranoside, namely, aspacochioside D (1) were isolated from Asparagus cochinchinensis (Lour.) Merr, along with three known saponins, aspacochioside C (2), (25S)-5β-spirostan-3β-yl-O-[O-α-L-rhamnopyranosyl-(1-4)]-β-D-glucopyranoside (3), and pseudoprotoneodioscin (4). The structure of 1 was elucidated on the basis of chemical reactions and spectral analysis (IR, GC, ESI-MS, ^1H-NMR, ^13C-NMR, DEPT, HMBC, HMQC and NOESY). The antiproliferative effects of 1-4 were evaluated in a cytotoxicity assay against the human tumor cell line, A549. Compound 2 (Aspacochioside C) exhibited moderate cytotoxicity against A-549, with an IC_50 value of 3.87 μg/mL.

Influence of Relative Humidity on the Temperature Increase of a Power Converter

Yang Xu,Hao Chen,Zhentao Hu,Dong Li 전력전자학회 2015 JOURNAL OF POWER ELECTRONICS Vol.15 No.3

As a vital part of renewable energy and electrical traction, power converters are supposed to have high reliability and good performance. However, power semiconductors produce considerable heat when the power converter works, which results in high junction temperatures that lower the reliability and performance of the power semiconductors. Many studies show that ambient humidity has a significant effect on power devices, but the influence of high humidity on junction temperatures has yet to be studied. Therefore, this paper presents a thermal model for power converters in moist air to obtain the junction temperature increase, which is utilized for the power converter used in a Switched Reluctance Motor System. Simulation results show that the law of converter temperature distribution is independent of the relative humidity in the case of fixed ambient temperature, whereas the temperature in the power converter decreases as the ambient relative humidity increases. These simulation results are validated with the experimental results.

Isochaetomium $A_2$, a new bis(naphthodihydropyran-4-one) with antimicrobial and immunological activities from fungus Chaetomium microcephalum

Xu, Guo-Bo,Yang, Tao,Bao, Jin-Ku,Fang, Dong-Mei,Li, Guo-You 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.5

Isochaetomium $A_2$ (1), a new bis(naphthodihydropyran-4-one), along with chaetochromins A (2) and B (3), was isolated from the solid-state fermented rice culture of Chaetomium microcephalum. The structure of compound 1 was elucidated on the basis of 1D and 2D NMR spectral data, and the relative configuration was confirmed by CD spectrum. Compounds 1-3 possessed significant antimicrobial activity against Escherichia coli 1.044, Staphylococcus aureus 1.252, and Bacillus subtilis 1.079. Moreover, compounds 1-3 showed obvious inhibitory effects on mouse spleen cell proliferation with successive $IC_{50}$ values of 0.52, 0.19, and $0.24{\mu}M$.

AIDS-Related Non-Hodgkin Lymphoma: Imaging Feature Analysis of 27 Cases and Correlation with Pathologic Findings

Yang, Jun,Wang, Peng,Lv, Zhi-Bin,Wei, Lian-Gui,Xu, Yun-Liang,Zhou, An,Xu, Dong-Hai,Ma, Da-Qing Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.18

Background: Some tumor types are related to HIV, including non-Hodgkin lymphoma (NHL). The morbidity and mortality of NHL has remained high, even after highly active antiretroviral therapy (HAART) was introduced. We collected cases of AIDS with NHL, and evaluated the imaging features and strategies for diagnosis. Materials and Methods: There were 27 patients with AIDS and tumors confirmed by pathology. There were 9 patients with Burkitt lymphoma, 16 with diffuse large B cell lymphomas (DLBCLs), and 2 with primary central nervous system (PCNS) lymphomas. All of the patients underwent a series of imaging studies. Three radiologists analyzed the images, and any disagreement was discussed until consensus was reached. Results: The radiologic manifestations of AIDS with NHL were mainly masses and lymphadenopathy, 3 patients having one mass and 12 two or more masses. 7 patients had lymphadenopathy in one site and 3patients had lymphadenopathy in two or more sites. Coarse mucosal folds, thickening of the gastrointestinal wall, and lumen narrowing were typical manifestations of NHL within the gastrointestinal tract. There were 4 patients with masses and 5 with lymphadenopathy inthe 9 with Burkitt lymphoma, and 11 patients with masses 5 with lymphadenopathy in the 16 with DLBCLs. Conclusion: NHL is a malignancy that usually occurs in patients with AIDS. Imaging is an important method by which to evaluate lesions, masses, and lymphadenopathy. Fine needle aspiration biopsy and stereotaxis biopsy are useful methods by which to diagnose NHL.