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        Characterization and mechanism analysis of penicillin G biodegradation with Klebsiella pneumoniae Z1 isolated from waste penicillin bacterial residue

        Pu Wang,Huiling Liu,Huan Fu,Xiuwen Cheng,Bing Wang,Qinghua Cheng,Jian Zhang,Pan Zou 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.27 No.-

        In this study, a strain Z-1 has been isolated from penicillin bacterial residue. The physicochemicalproperties and molecular phylogenetic analysis of the strain were characterized by atomic forcemicroscope (AFM) and scanning electron microscope (SEM) and 16SrDNA, indicating the strain belongsto genus Klebsiella pneumoniae. Furthermore, the biodegradation process of PENG was investigated with99.99% degradation efficiency. Afterwards, high performance liquid chromatography (HPLC) and LC-MS/MS analysis were performed to identify its degradation products. Moreover, the possible mechanism wasproposed and confirmed in detail. These results suggest Klebsiella pneumoniae Z1 become a promisingalternative for improving penicillin removal from environment

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        Controllable Preparation of Silver Orthophosphate@Carbon Layer Core/Shell Heterostructure with Enhanced Visible Photocatalytic Properties and Stability

        Yangcun Xie,Xiuwen Wang,Xu Wen 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2015 NANO Vol.10 No.2

        In this study, silver orthophosphate@carbon layer (Ag3PO4@C) core/shell heterostructure photocatalyst was prepared for the first time. The results showed that a uniform carbon layer was formed around the Ag3PO4. By adjusting the hydrothermal fabrication parameters, the thickness of carbon layer could be easily controlled. Furthermore, the Ag3PO4@C had remarkable light absorption in the visible region. Photocatalytic tests displayed that the Ag3PO4@C heterostructures possessed a much higher degradation rate of phenol than pure Ag3PO4 under visible light. The enhanced photocatalytic activity could be attributed to high separation efficiency of photogenerated electrons and holes based on the synergistic effect between carbon as a sensitizer and Ag3PO4. Recycle tests showed that the Ag3PO4@C core/shell heterostructures maintained high stability over several cycles. The good stability could be attributed to the protection of insoluble carbon layer on the surfaces of Ag3PO4 crystals in aqueous solution.

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        Whole genome sequencing of Luxi Black Head sheep for screening selection signatures associated with important traits

        Liu Zhaohua,Tan Xiuwen,Wang Jianying,Jin Qing,Meng Xianfeng,Cai Zhongfeng,Cui Xukui,Wang Ke 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.9

        Objective: Luxi Black Head sheep (LBH) is the first crossbreed specialized for meat production and was developed by crossbreeding Black Head Dorper sheep (DP) and Small Tailed Han sheep (STH) in the farming areas of northern China. Research on the genomic variations and selection signatures of LBH caused by continuous artificial selection is of great significance for identifying the genetic mechanisms of important traits of sheep and for the continuous breeding of LBH.Methods: We explored the genetic relationships of LBH, DP, and several Mongolian sheep breeds by constructing phylogenetic tree, principal component analysis and linkage disequilibrium analysis. In addition, we analysed 29 whole genomes of sheep. The genomewide selection signatures have been scanned with four methods: heterozygosity (HP), fixation index (FST), cross-population extended haplotype homozygosity (XP-EHH) and the nucleotide diversity (θπ) ratio.Results: The genetic relationships analysis showed that LBH appeared to be an independent cluster closer to DP. The candidate signatures of positive selection in sheep genome revealed candidate genes for developmental process (HoxA gene cluster, BCL2L11, TSHR), immunity (CXCL6, CXCL1, SKAP2, PTK6, MST1R), growth (PDGFD, FGF18, SRF, SOCS2), and reproduction (BCAS3, TRIM24, ASTL, FNDC3A). Moreover, two signalling pathways closely related to reproduction, the thyroid hormone signalling pathway and the oxytocin signalling pathway, were detected.Conclusion: The selective sweep analysis of LBH genome revealed candidate genes and signalling pathways associated with developmental process, immunity, growth, and reproduction. Our findings provide a valuable resource for sheep breeding and insight into the mechanisms of artificial selection.

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