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Guoliang Li,Yuanyuan Zhang,Huaning Zhang,Yujie Zhang,Lina Zhao,Zihui Liu,Xiulin Guo 한국식물학회 2019 Journal of Plant Biology Vol.62 No.5
Plant heat shock transcription factors (Hsfs) areimportant regulators of heat shock signal transduction pathway. There are 30 members of the Hsf family in maize, only twoof which, ZmHsf06 and ZmHsf12, belong to subclass A1. Our previous work demonstrated that ZmHsf06-overexpressingArabidopsis lines showed improved tolerance to heat anddrought stresses. In this study, we isolated ZmHsf12 fromyoung leaves of maize (Zea mays L.) using homologouscloning methods. The CDS (coding sequence) of ZmHsf12 is1,494 bp and encodes a putative protein consisting of 497amino acids which possesses domains such as DBD (DNAbindingdomain), OD (oligomerization domain), NLS (nuclearlocalization signal), NES (nuclear export signal), and anAHA (activator) motif. The ZmHsf12-GFP fusion protein islocalized to the cell nucleus. ZmHsf12 was expressed inmany maize organs, and its expression was up-regulated byheat shock. Furthermore, we characterized the function ofZmHsf12 in yeast and Arabidopsis. Yeast cells overexpressingZmHsf12 showed enhanced heat tolerance. ZmHsf12-overexpressing Arabidopsis seedlings displayed significantincreases in both basal and acquired thermotolerance. Comparedto WT seedlings, the ZmHsf12-overexpressing lines displayedboth increased chlorophyll contents and higher survivalrates. Also, the expression of AtHsps was increased higher inthe ZmHsf12-overexpressing Arabidopsis lines after heatstress. The results of our study strongly suggested thatZmHsf12 may take part in plant response to heat stress.
Synthesis of coryphantha elephantidens-like SnO2 nanospheres and their gas sensing properties
Xiao-xue Lian,Yan Li,Junwu Zhu,Yun-ling Zou,Xiulin Liu,Dongmin An,Qiong Wang 한국물리학회 2019 Current Applied Physics Vol.19 No.7
In this work, coryphantha elephantidens-like SnO2 with porous structures were prepared successfully by a simple hydrothermal route, through adjusting the temperature of hydrotherm. Its morphology was characterized by Xray diffraction (XRD), scanning electron microscopy (SEM) and Brunauer–Emmett–Teller (BET). Compared to the regular nanospheres, the coryphantha elephantidens-like SnO2 nanospheres had obviously higher gas-sensing response, owing to the special structure with large specific surface area (161.16m2 g−1). It surprised us that the coryphantha elephantidens-like SnO2 sensor could easily distinguish between ethanol and acetone, whose chemical property were similar. Moreover, it also exhibited wide measurement range, fast response speed (less than 10 s) and good repeatability at a low temperature (180 °C) to ethanol. The desirable specific surface area and pore volume were conducive to molecules adsorption and diffusion, which were believed to be the major cause of the improvement of gas sensing performance.