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Xu, Xiuling,Cao, Zhijian,Sheng, Jiqun,Wu, Wenlan,Luo, Feng,Sha, Yonggang,Mao, Xin,Liu, Hui,Jiang, Dahe,Li, Wenxin Korean Society for Biochemistry and Molecular Biol 2005 Journal of biochemistry and molecular biology Vol.38 No.4
Based on the reported cDNA sequences of $BmK{\alpha}Txs$, the genes encoding toxin $BmK{\alpha}Tx11$ and $BmK{\alpha}Tx15$ were amplified by PCR from the Chinese scorpion Buthus martensii Karsch genomic DNA employing synthetic oligonucleotides. Sequences analysis of nucleotide showed that an intron about 500 bp length interrupts signal peptide coding regions of $BmK{\alpha}Tx11$ and $BmK{\alpha}Tx15$. Using cDNA sequence of $BmK{\alpha}Tx11$ as probe, southern hybridization of BmK genome total DNA was performed. The result indicates that $BmK{\alpha}Tx11$ is multicopy genes or belongs to multiple gene family with high homology genes. The similarity of $BmK{\alpha}$-toxin gene sequences and southern hybridization revealed the evolution trace of $BmK{\alpha}$-toxins: $BmK{\alpha}$-toxin genes evolve from a common progenitor, and the genes diversity is associated with a process of locus duplication and gene divergence.
Zhang, Xiuling,Xu, Weiwei,Duan, Dongzhi,Park, Dong-Wha,Di, Lanbo IEEE 2018 IEEE transactions on plasma science Vol.46 No.8
<P>Atmospheric pressure (AP) oxygen cold plasma was adopted to synthesize Au/TiO<SUB>2</SUB> catalysts with the assistance of a simple modified incipient wetness impregnation process. Discharge voltages of 25.0, 29.0, and 35.0 kV and discharge time of 1, 2, and 4 min are selected to investigate the effects of discharge voltage and discharge time on the property of the Au/TiO<SUB>2</SUB> catalysts. The results of carbon monoxide (CO) oxidation activity testing show that AP oxygen cold plasma is beneficial to enhance the activity of the Au/TiO<SUB>2</SUB> catalysts, and the optimal discharge voltage and time are 29.0 kV and 1 min, respectively. CO conversion of the Au/TiO<SUB>2</SUB>-29-1 catalyst at 30 °C is 1.42 times as that of the Au/TiO<SUB>2</SUB>-As catalyst without plasma treatment. The UV-Vis diffuse reflectance spectroscopy and X-ray photoelectron spectroscopy characterizations indicate that AP oxygen cold plasma not only can decompose the gold species formed by the modified impregnation process to form metallic gold species but also can remove some of the oxygen species on the surface of the TiO<SUB>2</SUB> support. The discharge time of 1 min at 29.0 kV is enough to decompose the gold species formed by the modified impregnation process, and to keep much active hydroxyl groups. Moreover, there is little difference in the size of gold nanoparticles in the Au/TiO<SUB>2</SUB> catalysts. Therefore, Au/TiO<SUB>2</SUB>-29-1 catalyst exhibits higher CO oxidation activity than Au/TiO<SUB>2</SUB>-As, and 1 min is the optimal discharge time. Thanks to the AP oxygen cold plasma method, high-performance Au/TiO<SUB>2</SUB> catalysts can be synthesized simply in a short time.</P>
A real-time sorting algorithm for in-beam PET of heavy-ion cancer therapy device
Ke, Lingyun,Yan, Junwei,Chen, Jinda,Wang, Changxin,Zhang, Xiuling,Du, Chengming,Hu, Minchi,Yang, Zuoqiao,Xu, Jiapeng,Qian, Yi,She, Qianshun,Yang, Haibo,Zhao, Hongyun,Pu, Tianlei,Pei, Changxu,Su, Hong Korean Nuclear Society 2021 Nuclear Engineering and Technology Vol.53 No.10
A real-time digital time-stamp sorting algorithm used in the In-Beam positron emission tomography (In-Beam PET) is presented. The algorithm is operated in the field programmable gate array (FPGA) and a small amount of registers, MUX and memory cells are used. It is developed for sorting the data of annihilation event from front-end circuits, so as to identify the coincidence events efficiently in a large amount of data. In the In-Beam PET, each annihilation event is detected by the detector array and digitized by the analog to digital converter (ADC) in Data Acquisition Unit (DAQU), with a resolution of 14 bits and sampling rate of 50 MS/s. Test and preliminary operation have been implemented, it can perform a sorting operation under the event count rate up to 1 MHz per channel, and support four channels in total, count rate up to 4 MHz. The performance of this algorithm has been verified by pulse generator and <sup>22</sup>Na radiation source, which can sort the events with chaotic order into chronological order completely. The application of this algorithm provides not only an efficient solution for selection of coincidence events, but also a design of electronic circuit with a small-scale structure.
Fengnan Lian,Dan Wang,Shuo Yao,Lirui Ge,Yue Wang,Yuyi Zhao,Jinbin Zhao,Xiuling Song,Chao Zhao,Jinhua Li,Yajuan Liu,Minghua Jin,Kun Xu 한국식품과학회 2021 Food Science and Biotechnology Vol.30 No.8
This research aimed to detect Escherichia coli O157:H7 in milk based on immunomagnetic probe separation technology and quenching effect of gold nanoparticles to Rhodamine B. Streptavidin-modified magnetic beads (MBs) were combined with biotin-modified antibodies to capture E. coli O157:H7 specifically. Gold nanoparticle (AuNPs) was incubated with sulfhydryl-modified aptamers (SH-Aptamers) to obtain the Aptamers-AuNPs probe. After magnetic beads captured target bacteria and formed a sandwich structure with the gold nanoprobe, Rhodamine B was added into complex to obtain fluorescent signal changes. Our results demonstrated that the established method could detect E. coli O157:H7 in the range of 101–107 CFU/mL, and the limit of detection (LOD) was 0.35 CFU/mL in TBST buffer (pH = 7.4). In milk simulation samples, the LOD of this method was 1.03 CFU/mL. Our research provides a promising approach on the detection of E. coli O157:H7.