The First Case Report of JAK2–BCR–PPP1R32 Fusion Genes Because of a Translocation (9;22;11)(p24;q11.2;q13) in a Patient With Myeloproliferative Neoplasm

Xie Huan,Zhao Zhongyue,Zeng Dongfeng 대한진단검사의학회 2023 Annals of Laboratory Medicine Vol.43 No.3

Highly heat-resistant NF membrane modified by quinoxaline diamines for Li+ extraction from the brine

Huan-Yin Xie,Run-Hua Tang,Gui-E Chen,Zhen-Liang Xu,Haifang Mao 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.112 No.-

The Functional layers with excellent heat-resistence nanofiltration (HRN) property were designed by fixingthermal stabilized quinoxaline diamines (QHDA) on poly (m-phenyleneisophthalamide) (PMIA) substratethrough interfacial polymerization. QHDA was used as aqueous monomer while cinnamoylchloride (CNC), isophthaloyl chloride (IPC) and trimesoyl chloride (TMC) were served as organic monomersto realize the immobilization of thermally stable N heterocycles. The experimental results statedclearly that the compact package reaction of QHDA with TMC lead a raise of thermal stability and saltsrejection at high temperature stream. With the optimal TMC and 0.5 wt.% QHDA, the as-developed membraneachieved the excellent NF performance and thermal stability. The rejection of MgSO4 reached to94.6 % at 30 C and hardly decrease at 90 C. The reduction in rejection was satisfactory at high temperatures. The functional layer still showed highly stability after 10 h long-term operation at 90 C with only2.8 % reduction in MgSO4 rejection. Besides, the excellent Mg2+ and poor Li+ rejection made this membranehave huge potential in the application of extracting Li+ from brine. The separation factor ofMg2+-Li+ at high temperature was satisfactory. Over all, this study offered a prospect technology toexploit heat-resistant membranes for extraction lithium at high temperature.

VOCs transport with VOCs-adsorbing particles under VOCs emission from carpet

Huan Xie,Chang Nyung Kim,Baoqing Deng 대한기계학회 2016 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.30 No.8

Many kinds of Volatile organic compounds (VOCs) emitted from building materials can deteriorate indoor air quality. Thus, it is essential to investigate how VOCs transport and how the airside VOCs concentration can be reduced. In order to remove airside VOCs, activated carbon particles can be used. This paper investigates the effect of activated carbon particles on the airside concentration of VOCs emitted from a new carpet in a room. A new numerical method for predicting airside VOCs concentration under the presence of activated carbon particles is developed, which includes spatially repeated iteration to get the transient airside VOCs concentration in association with the activated carbon particles’ adsorption of VOCs. The current numerical model is validated over an existing experimental result. Commercial software FLUENT 12.0 is adopted to solve the continuity, momentum, turbulence and concentration equations in two main cases under the situations with and without considering particle deposition. Numerical results show that the transient concentrations of VOCs in the air reach peaks, decay rapidly, drop slowly and remain in the room for a long time. When the particle concentration is at a higher level, the decrease in airside VOCs concentration is more notable. The concept proposed in this study can be utilized, with the use of activated carbon particles, to enhance the emission rate of VOCs in raw building materials before they are furnished in newly built indoor spaces.

Adenovirus vector-mediated FAM176A overexpression induces cell death in human H1299 non-small cell lung cancer cells

( Hong Xie ),( Jia Hu ),( Huan Pan ),( Ya Xin Lou ),( Ping Lv ),( Ying Yu Chen ) 생화학분자생물학회 2014 BMB Reports Vol.47 No.2

FAM176A (family with sequence similarity 176 member A) is a novel molecule related to programmed cell death. A decreased expression of FAM176A has been found in several types of human tumors in including lung cancers. In the present study, we investigated the biological activities of FAM176A on the human non-small cell lung cancer cell line H1299 cells. We constructed a recombinant adenovirus 5-FAM176A vector (Ad5-FAM176A) and evaluated the expression and anti-tumor activities in vitro. Cell viability analysis revealed that the adenovirus-mediated increase of FAM176A inhibited the growth of the tumor cells in a dose- and time-dependent manner. This inhibitory effect was mediated by both autophagy and apoptosis that involved caspase activation. In addition, cell cycle analysis suggested that Ad5-FAM176A could induce cell cycle arrest at the G2/M phase, all of which suggested that adenovirus-mediated FAM176A gene transfer might present a new therapeutic approach for lung cancer treatment. [BMB Reports 2014; 47(2): 104-109]

DERIVED FROM INTERACTION: DEVELOPING A CUSTOMER ENGAGEMENT MODEL IN WELLNESS TOURISM

Lishan Xie,Xinhua Guan,Yingxin He,Tzung-Cheng (TC) Huan 글로벌지식마케팅경영학회 2018 Global Marketing Conference Vol.2018 No.07

To investigate the value co-creation process in wellness tourism, this study constructed a structural equation model of customer interactions with (1) the environment, (2) service employees, and (3) other customers relating to customer-perceived value and customer engagement. Empirical data were collected from 528 survey respondents who were at wellness tourism resorts. The results reveal that all three types of interaction have positive effects on customer-perceived value, and that perceived value positively affects customer engagement. Based on this finding, management recommendations for wellness tourism service enterprises are given.

Differences in the gut microbiota between Cercopithecinae and Colobinae in captivity

Zongjin Huan,Yongfang Yao,Jianqiu Yu,Hongwei Chen,Meirong Li,Chaojun Yang,Bo Zhao,Qingyong Ni,Mingwang Zhang,Meng Xie,Huailiang Xu 한국미생물학회 2020 The journal of microbiology Vol.58 No.5

The gut microbiome of captive primates can provide a window into their health and disease status. The diversity and composition of gut microbiota are influenced by not only host phylogeny, but also host diet. Old World monkeys (Cercopithecidae) are divided into two subfamilies: Cercopithecinae and Colobinae. The diet and physiological digestive features differ between these two subfamilies. Accordingly, highthroughput sequencing was used to examine gut microbiota differences between these two subfamilies, using data from 29 Cercopithecinae individuals and 19 Colobinae individuals raised in captivity. Through a comparative analysis of operational taxonomic units (OTUs), significant differences in the diversity and composition of gut microbiota were observed between Cercopithecinae and Colobinae. In particular, the gut microbiota of captive Old World monkeys clustered strongly by the two subfamilies. The Colobinae microbial diversity was higher than that of Cercopithecinae. Additionally, Firmicutes, Lactobacillaceae, Veillonellaceae, and Prevotella abundance were higher in Cercopithecinae, while Bacteroidetes, Ruminococcaceae, Christensenellaceae, Bacteroidaceae, and Acidaminococcaceae abundance were higher in Colobinae. PICRUSt analysis revealed that the predicted metagenomes of metabolic pathways associated with proteins, carbohydrates, and amino acids were significantly higher in Colobinae. In the context of host phylogeny, these differences between Cercopithecinae and Colobinae could reflect adaptations associated with their respective diets. This well-organized dataset is a valuable resource for future related research on primates and gut microbiota. Moreover, this study may provide useful insight into animal management practices and primate conservation.

Five-Year Follow-Up Results of a Randomized Controlled Trial Comparing Bipolar Plasmakinetic and Monopolar Transurethral Resection of the Prostate

Chang-Ying Xie,Xing-Huan Wang,Guang-Bin Zhu,Xiang-Bin Liu 연세대학교의과대학 2012 Yonsei medical journal Vol.53 No.4

Purpose: To report the 5-year follow-up results of a randomized controlled trial comparing bipolar transurethral resection of the prostate (TURP) with standard monopolar TURP for the treatment of benign prostatic obstruction (BPO). Materials and Methods: A total of 220 patients were randomized to bipolar plasmakinetic TURP (PK-TURP) or monopolar TURP (M-TURP). Catheterization time was the primary endpoint of this study. Secondary outcomes included operation time, hospital stay, as well as decline in postoperative serum sodium and hemoglobin levels. All patients were assessed preoperatively and followed-up at 1, 6, 12, 24, 36, 48, and 60 months postoperatively. Parameters assessed included quality of life, transrectal ultrasound, serum prostate-specific antigen level, postvoid residual urine volume, maximum urinary flow rates (Qmax), and International Prostate Symptom Score. Patient baseline characteristics, perioperative data including complications, and postoperative outcomes were compared. Complication occurrence was graded according to the modified Clavien classification system. Results: PK-TURP was significantly superior to M-TURP in terms of operation time, intraoperative irrigation volume, resected tissue weight, decreases in hemoglobin and sodium, postoperative irrigation volume and time, catheterization time, and hospital stay. At 5 years postoperatively, efficacy was comparable between arms. No differences were detected in safety outcomes except that the clot retention rate was significantly greater after M-TURP. Conclusion: Our results indicate that PK-TURP is equally as effective in the treatment of BPO, but has a more favorable safety profile in comparison to M-TURP. The clinical efficacy of PK-TURP is long-lasting and comparable with M-TURP.

Evaluation of suitable qRT-PCR normalization genes for various citrus rootstocks

He Wen,Xie Rui,Li Huan,Wang Yan,Chen Qing,Lin Yuanxiu,Zhang Yunting,Luo Ya,Zhang Yong,Tang Haoru,Wang Xiaorong 한국식물생명공학회 2022 Plant biotechnology reports Vol.16 No.1

Citrus rootstock can modify plant growth and enhance stress resistance. There are many genotypes and species used as citrus rootstocks. Although multiple citrus rootstocks whole genome sequence and transcriptome databases have previously been published, no suitable internal reference genes have been investigated for standardization of gene expression via quantitative real-time PCR. Here we reported the first systematic and comprehensive analysis of reference genes for quantitative real- time PCR standardization in various citrus rootstocks. The expression stability of ten candidate reference genes in diverse sample subsets of flooding, drought, alkaline and cold treatments was evaluated using four statistical algorithms, geNorm, NormFinder, BestKeeper and ΔCt method. The results demonstrated that the expression stability of reference genes varied under different experimental conditions. In addition, normalization of gene expression of 9-cis-epoxycarotenoid dioxygenase 3 (NCED 3), involved in abscisic acid biosynthesis, was conducted to further confirm the reliability of the reference genes. Overall, EF-1α, DIM 1, GAPC and UBC expressed much more stably. ACTIN and GAPDH were not recommended for normalization in given experimental conditions due to low stability. Our main contribution was to identify reference genes with suitable and stable expression in citrus rootstocks varied across selected experimental conditions. Furthermore, these results will provide valuable information for future research on citrus rootstocks.

Systemic Analysis on Risk Factors for Breast Cancer Related Lymphedema

Zhu, Ya-Qun,Xie, Yu-Huan,Liu, Feng-Huan,Guo, Qi,Shen, Pei-Pei,Tian, Ye Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.16

Background: To evaluate risk factors for upper extremity lymphedema due to breast cancer surgery. Materials and Methods: Clinical studies published on PubMed, Ovid, EMbase, and Cochrane Library from January 1996 to December 2012 were selected. Results: Twenty-five studies were identified, including 12,104 patients. Six risk factors related to the incidence of lymphedema after breast cancer treatment were detected: axillary lymph node dissection (OR=3.73, 95%CI 1.16 to 11.96), postoperative complications (OR=2.64, 95%CI 1.10 to 6.30), hypertension (OR=1.83, 95%CI 1.38 to 2.42), high body mass index (OR=1.80, 95%CI 1.30 to 2.49), chemotherapy (OR=1.38, 95%CI 1.07 to 1.79) and radiotherapy (OR=1.35, 95%CI 1.10 to 1.66). We found significant protective factors for lymphedema: pathologic T classification (OR=0.57, 95%CI 0.36 to 0.91) and stage (OR=0.60, 95%CI 0.39 to 0.93), while some factors, like age, number of positive lymph nodes, number of lymph node dissection, demonstrated no obvious correlation. Conclusions: Axillary lymph node dissection, postoperative complications, hypertension, body mass index, chemotherapy, radiotherapy are risk factors for lymphedema after breast cancer treatment. Attention should be paid to patients with risk factors to prevent the occurrence of lymphedema.

Distinct Transcriptional and Functional Differences of Lung Resident and Monocyte-Derived Alveolar Macrophages During the Recovery Period of Acute Lung Injury

Hou Fei,Wang Huan,Zheng Kun,Yang Wenting,Xiao Kun,Rong Zihan,Xiao Junjie,Li Jing,Cheng Baihe,Tang Li,Xie Lixin 대한면역학회 2023 Immune Network Vol.23 No.3

In acute lung injury, two subsets of lung macrophages exist in the alveoli: tissue-resident alveolar macrophages (AMs) and monocyte-derived alveolar macrophages (MDMs). However, it is unclear whether these 2 subsets of macrophages have different functions and characteristics during the recovery phase. RNA-sequencing of AMs and MDMs from the recovery period of LPS-induced lung injury mice revealed their differences in proliferation, cell death, phagocytosis, inflammation and tissue repair. Using flow cytometry, we found that AMs showed a higher ability to proliferate, whereas MDMs expressed a larger amount of cell death. We also compared the ability of phagocytosing apoptotic cells and activating adaptive immunity and found that AMs have a stronger ability to phagocytose, while MDMs are the cells that activate lymphocytes during the resolving phase. By testing surface markers, we found that MDMs were more prone to the M1 phenotype, but expressed a higher level of pro-repairing genes. Finally, analysis of a publicly available set of single-cell RNA-sequencing data on bronchoalveolar lavage cells from patients with SARS-CoV-2 infection validated the double-sided role of MDMs. Blockade of inflammatory MDM recruitment using CCR2−/− mice effectively attenuates lung injury. Therefore, AMs and MDMs exhibited large differences during recovery. AMs are long-lived M2-like tissue-resident macrophages that have a strong ability to proliferate and phagocytose. MDMs are a paradoxical group of macrophages that promote the repair of tissue damage despite being strongly pro-inflammatory early in infection, and they may undergo cell death as inflammation fades. Preventing the massive recruitment of inflammatory MDMs or promoting their transition to pro-repairing phenotype may be a new direction for the treatment of acute lung injury.