HGAL, a germinal center specific protein, decreases lymphoma cell motility by modulation of the RhoA signaling pathway

Jiang, Xiaoyu,Lu, Xiaoqing,McNamara, George,Liu, Xiaofei,Cubedo, Elena,Sarosiek, Kristopher A.,Sá,nchez-Garcí,a, Isidro,Helfman, David M.,Lossos, Izidore S. American Society of Hematology 2010 Blood Vol.116 No.24

<B>Abstract</B><P>HGAL is a germinal center (GC)-specific gene that negatively regulates lymphocyte motility and whose expression predicts improved survival of patients with diffuse large B-cell lymphoma (DLBCL) and classical Hodgkin lymphoma (cHL). We demonstrate that HGAL serves as a regulator of the RhoA signaling pathway. HGAL enhances activation of RhoA and its down-stream effectors by a novel mechanism - direct binding to the catalytic DH-domain of the RhoA-specific guanine nucleotide exchange factors (RhoGEFs) PDZ-RhoGEF and LARG that stimulate the GDP-GTP exchange rate of RhoA. We delineate the structural domain of HGAL that mediates its interaction with the PDZ-RhoGEF protein. These observations reveal a novel molecular mechanism underlying the inhibitory effects of GC-specific HGAL protein on the motility of GC-derived lymphoma cells. This mechanism may underlie the limited dissemination and better outcome of patients with HGAL-expressing DLBCL and cHL.</P>

Study on the Influencing Factors of TFP of Low-carbon Tourism Distribution

Xiaoyu Cheng,Keshen Jiang 한국유통과학회 2017 The Journal of Industrial Distribution & Business( Vol.8 No.7

Purpose - Performance appraisal has a significant influence on the development of low-carbon tourism distribution. Research design, data, and methodology - Data of this study are collected from 27 provinces (cities) of China. SBM-Malmquist model is used to measure the TFP and its dynamic changes of low-carbon tourism distribution; TOBIT model is used to discuss the factors of TFP of low-carbon tourism distribution. Results - The results show that, there are obvious differences among regional TFP of low-carbon tourism distribution, the average change tends to grow positively in general, and the western region grows fastest on average due to the improvement of technical efficiency and technical progress, while there are technical efficiency improvement but technical regresses in eastern and central regions. The economic scale, economic strength, structure of energy consumption, location quotient and government regulation have a significant positive effect on the TFP of low-carbon tourism; energy intensity, industrial structure and opening degree have a negative effect; investments in fixed assets, intensity of R&D fund and urbanization rate have no significant influence on the TFP of low-carbon tourism. Conclusions - Improving the productivity of low-carbon tourism and reducing regional differences are effective ways to develop low-carbon tourism and enhance tourism competitiveness.

Study on Tourism Carbon Emissions and Distribution Efficiency of Tourism Economics

Xiaoyu Cheng,Keshen Jiang 한국유통과학회 2018 Asian Journal of Business Environment (AJBE) Vol.8 No.2

Purpose - It is important to figure out the relationship between tourism carbon emissions and tourism economics for a healthy tourism development. Research design, data, and methodology - Data of this study are collected from 27 provinces (cities) of China. Tourist consumption stripping coefficient is used to calculate tourism carbon emissions. SBM-Undesirable model is used to measure the efficiency of tourism economics under the constraint of tourism carbon emissions. Results - The results show that: during the year of 2005-2015, there are obvious differences in totals and intensities of tourism carbon emissions among 27 provinces and cities which can be divided into three areas. There is a high possibility of underestimating the actual efficiency of tourism economics by leaving tourism carbon emissions out of account, and a high inefficiency caused by tourism carbon emissions will lead a low efficiency of tourism economics. Conclusions - The development of tourism should give consideration to both economic and environmental benefits, and reduce the inefficiency caused by tourism carbon emissions to improve efficiency of tourism economics by improving the level of technical efficiency and promoting technological progress.

Acute Effects of Transforming Growth Factor-β1 on Neuronal Excitability and Involvement in the Pain of Rats with Chronic Pancreatitis

( Xiaoyu Zhang ),( Hang Zheng ),( Hong-yan Zhu ),( Shufen Hu ),( Shusheng Wang ),( Xinghong Jiang ),( Guang-yin Xu ) 대한소화기기능성질환·운동학회 2016 Journal of Neurogastroenterology and Motility (JNM Vol.22 No.2

Background/Aims This study was to investigate whether transforming growth factor-β1 (TGF-β1) plays a role in hyperalgesia in chronic pancreatitis (CP) and the underlying mechanisms. Methods CP was induced in male adult rats by intraductal injection of trinitrobenzene sulfonic acid (TNBS). Abdominal hyperalgesia was assessed by referred somatic behaviors to mechanical stimulation of rat abdomen. Dil dye injected into the pancreas was used to label pancreas-specific dorsal root ganglion (DRG) neurons. Whole cell patch clamp recordings and calcium imaging were performed to examine the effect of TGF-β1 on acutely isolated pancreas-specific DRG neurons. Western blot analysis was carried out to measure the expression of TGF-β1 and its receptors. Results TNBS injection significantly upregulated expression of TGF-β1 in the pancreas and DRGs, and TGF-β1 receptors in DRGs (T9-T13) in CP rats. Intrathecal injection of TGF-β receptor I antagonist SB431542 attenuated abdominal hyperalgesia in CP rats. TGF-β1 application depolarized the membrane potential and caused firing activity of DRG neurons. TGF-β1 application also reduced rheobase, hyperpolarized action potential threshold, and increased numbers of action potentials evoked by current injection of pancreas-specific DRG neurons. TGF-β1 application also increased the concentration of intracellular calcium of DRG neurons, which was inhibited by SB431542. Furthermore, intrathecal injection of TGF-β1 produced abdominal hyperalgesia in healthy rats. Conclusions These results suggest that TGF-β1 enhances neuronal excitability and increases the concentration of intracellular calcium. TGF-β1 and its receptors are involved in abdominal hyperalgesia in CP. This and future study might identify a potentially novel target for the treatment of abdominal pain in CP. (J Neurogastroenterol Motil 2016;22:333-343)

Iron-doped chitosan microsphere for As(III) adsorption in aqueous solution: Kinetic, isotherm and thermodynamic studies

Xiaoyu Lin,Leli Wang,Shi Jiang,Longzhe Cui,Gui-Ping Wu 한국화학공학회 2019 Korean Journal of Chemical Engineering Vol.36 No.7

Iron-doped chitosan microsphere was prepared successfully and employed for effective adsorption of As(III). The results showed that the adsorption capacity benefited from the increase of iron content, and the maximum adsorption capacity was achieved at pH=8. According to the study of adsorption kinetics, adsorption rate was controlled by liquid film diffusion at a lower rotational speed, while it was controlled by chemical reaction rate at a higher rotational speed. The Freundlich and Temkin models exhibited a better fit to adsorption isotherm data, which indicated the adsorption of As(III) on iron-doped chitosan microsphere was chemisorption and the active sites of adsorbents were non-uniform distributed. Adsorption process was a spontaneous exothermic reaction because its ΔG and ΔH were negative. In presence of cations (Cd2+, Pb2+ or Zn2+) in solution, the iron-doped chitosan microsphere also showed the significant removal of As(III). However, the existence of anions (NO3 , SO4 2 or PO4 3) inhibited the As(III) removal at different level. PO4 3 showed the most significant side effects on the removal of As(III) by iron-doped chitosan microsphere. The used iron-doped chitosan adsorbent can be effectively regenerated using 1.0mol·L1 NaOH solution, and the adsorption efficiency decreased only 15.69% after being reused three times. The results of XPS, FT-IR showed that the adsorption was mainly achieved by the coordination interaction between As (III) and doped Fe in adsorbent.

Integration of hemicellulose pre-extraction and solid alkali-oxygen cooking processes for lignocellulose fractionation with emphasis on xylan valorization

Yetao Jiang,Xiaoyu Wang,Zhen Wu,Jiaxing Xu,Lei Hu,Lu Lin 한국화학공학회 2021 Korean Journal of Chemical Engineering Vol.38 No.4

Cooking with active oxygen and solid alkali (CAOSA) is a mild and environmentally friendly method for separation of lignin components. The degradation mechanism of hemicellulose associated with this method has been investigated. It was found that the hemicellulose-derived polysaccharides were directly oxidized rather than hydrolyzed to monosaccharides for degradation. To avoid the undesirable degradation of hemicellulose components by CAOSA, the idea of separating hemicellulose first in the form of xylan to produce xylooligosaccharide was presented by this study. Although hydrothermal autocatalysis has been commonly used in hemicellulose separation, the effects of acidenhanced hydrothermal method and alkali-suppressed hydrothermal method on hydrothermal autocatalysis were systematically compared for the first time, and it was found that the acid-enhanced method was beneficial for highly selective extraction of xylan. Then, the hemicellulose-removed bamboo was treated with CAOSA process to separate lignin from cellulose. It was found that solid alkali dosage of CAOSA could be evidently decreased for hemicelluloseremoved bamboo, which was helpful to reduce the cost of CAOSA. Coupling hemicellulose pre-extraction and CAOSA lignin fractionation strategy, a comprehensive biorefinery case for bamboo biomass could be anticipated.

Implications of Sarcopenia and Glucometabolism Parameters of Muscle Derived From Baseline and End-of-Treatment 18F-FDG PET/CT in Diffuse Large B-Cell Lymphoma

Tan Xiaoyue,Sun Xiaolin,Chen Yang,Wang Fanghu,Shang Yuxiang,Zhang Qing,Yuan Hui,Jiang Lei 대한영상의학회 2024 Korean Journal of Radiology Vol.25 No.3

Objective: We previously found that the incidence of sarcopenia increased with declining glucose metabolism of muscle in patients with treatment-naïve diffuse large B-cell lymphoma (DLBCL). This study aimed to investigate the relationship between sarcopenia and muscle glucometabolism using 18F-FDG PET/CT at baseline and end-of-treatment, analyze the changes in these parameters through treatment, and assess their prognostic values. Materials and Methods: The records of 103 patients with DLBCL (median 54 years [range, 21–76]; male:female, 50:53) were retrospectively reviewed. Skeletal muscle area at the third lumbar vertebral (L3) level was measured, and skeletal muscle index (SMI) was calculated to determine sarcopenia, defined as SMI < 44.77 cm2/m2 and < 32.50 cm2/m2 for male and female, respectively. Glucometabolic parameters of the psoas major muscle, including maximum standardized uptake value (SUVmax) and mean standardized uptake value (SUVmean), were measured at L3 as well. Their changes across treatment were also calculated as ΔSMI, ΔSUVmax, and ΔSUVmean; Δbody mass index was also calculated. Associations between SMI and the metabolic parameters were analyzed, and their associations with progression-free survival (PFS) and overall survival (OS) were identified. Results: The incidence of sarcopenia was 29.1% and 36.9% before and after treatment, respectively. SMI (P = 0.004) was lower, and sarcopenia was more frequent (P = 0.011) at end-of-treatment than at baseline. The SUVmax and SUVmean of muscle were lower (P < 0.001) in sarcopenia than in non-sarcopenia at both baseline and end-of-treatment. ΔSMI was positively correlated with ΔSUVmax of muscle (P = 0.022). Multivariable Cox regression analysis showed that sarcopenia at end-of-treatment was independently negatively associated with PFS (adjusted hazard ratio [95% confidence interval], 2.469 [1.022–5.965]), while sarcopenia at baseline was independently negatively associated with OS (5.051 [1.453–17.562]). Conclusion: Sarcopenic patients had lower muscle glucometabolism, and the muscular and metabolic changes across treatment were positively correlated. Sarcopenia at baseline and end-of-treatment was negatively associated with the prognosis of DLBCL.

Pd nanoparticles immobilized on TiO2 nanotubes-functionalized ceramic membranes for flow-through catalysis

Jianfeng Miao,Xiaoyue Liu,Hong Jiang,Yefei Liu,Rizhi Chen 한국화학공학회 2019 Korean Journal of Chemical Engineering Vol.36 No.3

A high performance catalytic membrane was fabricated with Pd nanoparticles supported by TiO2 nanotubes, where the TiO2 nanotubes were synthesized on the ceramic membrane via a simple hydrothermal etching. A flow-through catalytic membrane reactor was developed for testing the catalytic properties in the p-nitrophenol reduction. The effect of etching time was investigated in detail and an optimal etching time was determined to be 16 h. The characterization results highlighted that the as-prepared bouquet-like TiO2 nanotubes could significantly improve the loading amount and dispersity of Pd nanoparticles. The fabricated catalytic membrane exhibited considerably improved catalytic activity and stability, with a 100% conversion of p-nitrophenol and no loss in catalytic activity during five reaction cycles. The obtained activation energy was much lower than the values in literatures, implying that the p-nitrophenol reduction could take place more easily on our catalytic membranes compared to other catalysts.

Electrochemical properties of stacked-nanoflake Li4Ti5O12 spinel synthesized by a polymer-pyrolysis method

Xiaoming Zhu,Xiaoyu Jiang,Haiyan Lu,Xinping Ai,Hanxi Yang,Yuliang Cao 한국물리학회 2014 Current Applied Physics Vol.14 No.4

Stacked-nanoflake Li4Ti5O12 spinel was synthesized via the pyrolysis of a LieTi copolymeric precursor formed by in situ polymerization of LiOH and [Ti(OC4H9)4] and acrylic acid. XRD and SEM characterization shows that the powders calcined at 700 C for 3 h was well-crystallized particles with submicron diameter. Chargeedischarge measurement showed the Li4Ti5O12 electrode had displayed excellent rate capability and delivered reversible capacity of 171, 158, 148, 138 and 99 mAh g1 at rates of 0.1C, 0.5C, 1C, 2C and 4C, respectively. The test electrode also showed excellent cyclability as the capacity retains 96.1% after 60 cycles between 0.5 and 2.5 V.

Nanophase ZnV2O4 as stable and high capacity Li insertion electrode for Li-ion battery

Xiaoming Zhu,Xiaoyu Jiang,Lifen Xiao,Xinping Ai,Hanxi Yang,Yuliang Cao 한국물리학회 2015 Current Applied Physics Vol.15 No.4

Spinel ZnV2O4 nanoparticles are synthesized by a hydrothermal method and its properties are characterized using XRD, SEM, TEM, and electrochemical test. The structural and morphological characterizations show that ZnV2O4 sample has high purity and well crystallization with crystal size less than 20 nm. The as prepared electrode shows stable capacity over 660 mAh g-1 in the voltage range of 0.01-3.0 V at 50 mA g-1. The reaction mechanism with lithium ion is also investigated through ex-XRD and -TEM. It shows that the pristine ZnV2O4 is transformed to isostructural spinel LixV2O4 (x close to 7.6) and metal Zn phase during the first lithiation process. Then the spinel LixV2O4 seems to perform a topotactic intercalation reaction mechanism and that the in-situ formed LixV2O4 can still keep its spinel matrix while allowing more than 5.7 lithium reversibly into/out over 50 cycles.