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RISS 인기검색어
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- 저자 : Xiaokun Li (검색결과 3 건)
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Expression of Human FGF18 by Fusion with Oleosin in Arabidopsis thaliana Seeds
Chao Jiang,Xiaokun Li,Feng Zhai,Nuo Xu,Jing Yang,Yunpeng Wang,Libo Jin,Haiyan Li 한국식물학회 2018 Journal of Plant Biology Vol.61 No.3
Expression of recombinant human fibroblast growthfactor 18 (hFGF18) in mammalian cells and Escherichia colihas been extensively used for fundamental research andclinical applications, but they are difficult, expensive. Theexpression of recombinant proteins fused to oleosin proteinhave distinct advantages, such as safety, ease, low cost. Sowe have expressed hFGF18 fused to oleosin protein in the oilbodies of Arabidopsis thaliana (A. thaliana) and screen theproliferation effect of NIH3T3 cells. The vector of oleosinhFGF18fusion gene was constructed and transformed intowild A. thaliana. Transformed A. thaliana lines were obtainedby the floral dip method and confirmed using polymerasechain reaction (PCR). The PCR results indicated that theoleosin-hFGF18 fusion gene was integrated into the A. thaliana genome. The oil bodies expression of oleosin-hFGF18was confirmed by sodium dodecyl sulfate polyacrylamidegel electrophoresis and western blotting. The biologicalactivity showed that oil bodies expressing oleosin-hFGF18could stimulate the proliferation of NIH3T3 cells.
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Experimental investigation of multi-layered laminated glass beams under in-plane bending
Qiang Liu,Xiaokun Huang,Gang Liu,Zhen Zhou,Gang Li 국제구조공학회 2016 Structural Engineering and Mechanics, An Int'l Jou Vol.60 No.5
Due to its relatively good safety performance and aesthetic benefits, laminated glass (LG) is increasingly being used as load-carrying members in modern buildings. This paper presents an experimental study into one applicational scenario of structural LG subjected to in-plane bending. The aim of the study is to reveal the in-plane behaviors of the LG beams made up of multi-layered glass sheets. The LG specimens respectively consisted of two, three and four plies of glass, bonded together by two prominent adhesives. A total of 26 tests were carried out. From these tests, the structural behaviors in terms of flexural stiffness, load resistance and post-breakage strength were studied in detail, whilst considering the influence of interlayer type, cross-sectional interlayer percentage and presence of shear forces. Based on the test results, analytical suggestions were made, failure modes were identified, corresponding failure mechanisms were discussed, and a rational engineering model was proposed to predict the post-breakage strength of the LG beams. The results obtained are expected to provide useful information for academic and engineering professionals in the analysis and design of LG beams bending in-plane.
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Ag-W18O49-GO Nanocomposite as Highly Effective Antibacterial Agent with Capturing-Killing Mechanism
Le Xu,Linge Chai,Zhao Li,Xiaokun Jin,Danxia Gu,Xueting Chang,Shibin Sun 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2017 NANO Vol.12 No.12
In this work, a novel nanocomposite (NC) based on the decoration of Ag nanoparticles (NPs) and W18O49 nanorods (NRs) on the GO surfaces was prepared using combined techniques of solvothermal method and photochemical process. Compared with the Ag-GO NC, the Ag-W18O49-GO NC exhibited enhanced long-term antibacterial activity against the bacterium of Bacillus sp.1NLA3E (Bacillus sp.). The antibacterial mechanisms of both the Ag-GO and Ag-W18O49-GO NCs were distinctly revealed by observation of the direct interaction between the Ag NPs,W18O49 NRs, or GO sheets and the bacterial cells. Results showed that the GO sheets in the NCs mainly acted as supports for trapping or wrapping the bacterial cells, and it was the Ag NPs or W18O49 NRs grown on the GO surfaces that caused direct damage to the cells. The synergistic effects between the Ag NPs or W18O49 NRs and the GO sheets resulted in the prominent long-term antibacterial effect of the Ag-W18O49-GO NC. This work provides in-depth understanding of the mechanism regarding the antibacterial activity of the GO-based NCs.
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