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RISS 인기검색어
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- 저자 : Libo Jin (검색결과 3 건)
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Peng, Jinliang,Garcia, Mitch André,Choi, Jin-sil,Zhao, Libo,Chen, Kuan-Ju,Bernstein, James R.,Peyda, Parham,Hsiao, Yu-Sheng,Liu, Katherine W.,Lin, Wei-Yu,Pyle, April D.,Wang, Hao,Hou, Shuang,Tse American Chemical Society 2014 ACS NANO Vol.8 No.5
<P/><P>Substrate-mediated gene delivery is a promising method due to its unique ability to preconcentrate exogenous genes onto designated substrates. However, many challenges remain to enable continuous and multiround delivery of the gene using the same substrates without depositing payloads and immobilizing cells in each round of delivery. Herein we introduce a gene delivery system, nanosubstrate-mediated delivery (NSMD) platform, based on two functional components with nanoscale features, including (1) DNA⊂SNPs, supramolecular nanoparticle (SNP) vectors for gene encapsulation, and (2) Ad-SiNWS, adamantane (Ad)-grafted silicon nanowire substrates. The multivalent molecular recognition between the Ad motifs on Ad-SiNWS and the β-cyclodextrin (CD) motifs on DNA⊂SNPs leads to dynamic assembly and local enrichment of DNA⊂SNPs from the surrounding medium onto Ad-SiNWS. Subsequently, once cells settled on the substrate, DNA⊂SNPs enriched on Ad-SiNWS were introduced through the cell membranes by intimate contact with individual nanowires on Ad-SiNWS, resulting in a highly efficient delivery of exogenous genes. Most importantly, sequential delivery of multiple batches of exogenous genes on the same batch cells settled on Ad-SiNWS was realized by sequential additions of the corresponding DNA⊂SNPs with equivalent efficiency. Moreover, using the NSMD platform <I>in vivo</I>, cells recruited on subcutaneously transplanted Ad-SiNWS were also efficiently transfected with exogenous genes loaded into SNPs, validating the <I>in vivo</I> feasibility of this system. We believe that this nanosubstrate-mediated delivery platform will provide a superior system for <I>in vitro</I> and <I>in vivo</I> gene delivery and can be further used for the encapsulation and delivery of other biomolecules.</P>
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Expression of Human FGF18 by Fusion with Oleosin in Arabidopsis thaliana Seeds
Chao Jiang,Xiaokun Li,Feng Zhai,Nuo Xu,Jing Yang,Yunpeng Wang,Libo Jin,Haiyan Li 한국식물학회 2018 Journal of Plant Biology Vol.61 No.3
Expression of recombinant human fibroblast growthfactor 18 (hFGF18) in mammalian cells and Escherichia colihas been extensively used for fundamental research andclinical applications, but they are difficult, expensive. Theexpression of recombinant proteins fused to oleosin proteinhave distinct advantages, such as safety, ease, low cost. Sowe have expressed hFGF18 fused to oleosin protein in the oilbodies of Arabidopsis thaliana (A. thaliana) and screen theproliferation effect of NIH3T3 cells. The vector of oleosinhFGF18fusion gene was constructed and transformed intowild A. thaliana. Transformed A. thaliana lines were obtainedby the floral dip method and confirmed using polymerasechain reaction (PCR). The PCR results indicated that theoleosin-hFGF18 fusion gene was integrated into the A. thaliana genome. The oil bodies expression of oleosin-hFGF18was confirmed by sodium dodecyl sulfate polyacrylamidegel electrophoresis and western blotting. The biologicalactivity showed that oil bodies expressing oleosin-hFGF18could stimulate the proliferation of NIH3T3 cells.
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Chandrasekaran, Sundaram,Bowen, Chris,Roscow, James,Zhang, Yan,Dang, Dinh Khoi,Kim, Eui Jung,Misra, R.D.K.,Deng, Libo,Chung, Jin Suk,Hur, Seung Hyun Elsevier 2019 Physics reports Vol.792 No.-
<P><B>Abstract</B></P> <P>This comprehensive review focuses on recent advances in energy harvesting of micro-scale and nano-scale generators based on piezoelectric and triboelectric effects. The development of flexible and hybrid devices for a variety of energy harvesting applications are systematically reviewed. A fundamental understanding of the important parameters that determine the performance of piezoelectric, triboelectric and hybrid devices are summarized. Current research directions being explored and the emerging factors to improve harvester functionality and advance progress in achieving high performance and durable energy conversion are provided. Investigations with regard to integrating flexible matrices and optimizing the composition of the piezoelectric and triboelectric materials are examined to enhance device performance and improve cost-effectiveness for the commercial arena. Finally, future research trends, emerging device structures and novel materials in view of imminent developments and harvesting applications are presented.</P>
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