Effect of High Dietary Carbohydrate on the Growth Performance, Blood Chemistry, Hepatic Enzyme Activities and Growth Hormone Gene Expression of Wuchang Bream (Megalobrama amblycephala) at Two Temperatures

Chuanpeng Zhou,Xianping Ge,Bo Liu,Jun Xie,Ruli Chen,Mingchun Ren 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.2

The effects of high carbohydrate diet on growth, serum physiological response, and hepatic heat shock protein 70 expression in Wuchang bream were determined at 25°C and 30°C. At each temperature, the fish fed the control diet (31% CHO) had significantly higher weight gain, specific growth rate, protein efficiency ratio and hepatic glucose-6-phosphatase activities, lower feed conversion ratio and hepatosomatic index (HSI), whole crude lipid, serum glucose, hepatic glucokinase (GK) activity than those fed the high-carbohydrate diet (47% CHO) (p<0.05). The fish reared at 25°C had significantly higher whole body crude protein and ash, serum cholesterol and triglyceride, hepatic G-6-Pase activity, lower glycogen content and relative levels of hepatic growth hormone (GH) gene expression than those reared at 30°C (p<0.05). Significant interaction between temperature and diet was found for HSI, condition factor, hepatic GK activity and the relative levels of hepatic GH gene expression (p<0.05).

NINJ1 triggers extravillous trophoblast cell dysfunction through blocking the STAT3 signaling pathway

Zhang Xueluo,Chen Yanhua,Wang Xianping,Zhang Zhiping,Wang Jun,Shen Yan,Hu Yuanjing,Wu Xueqing 한국유전학회 2022 Genes & Genomics Vol.44 No.11

Background: Trophoblasts are the most important parts of the placenta in early pregnancy. Trophoblast cell dysfunction can induce embryo implantation insufficiency, thereby resulting in multiple diseases, including recurrent spontaneous abortion (RSA). A previous study indicates higher nerve injury-induced protein 1 (NINJ1) RNA levels in the villi tissues of RSA patients. Objective: This study aimed to investigate the effect of NINJ1 on trophoblast behaviors and pregnancy loss. Methods: Fresh villi tissues were obtained from with RSA patients and patients with artificial selective abortion for personal reasons, and NINJ1 expression in these tissues was detected. Extravillous trophoblast cell line HTR-8/SVneo was transfected with small-interfering RNA targeting NINJ1 or NINJ1 overexpression vector to perform loss-/gain-of-function experiments. Spontaneous abortion (SA) was induced by mating CBA/J females with DBA/2 males and the pregnant females were intraperitoneally injected with adenovirus vector carrying NINJ1 short hairpin RNA. Results: NINJ1 mRNA and protein levels were higher in the villi tissues of RSA patients than those of artificial selective abortion patients. NINJ1 knockdown promoted trophoblast cell proliferation, migration and invasion but inhibited cell apoptosis. Moreover, conditioned medium from NINJ1-depleted trophoblasts promoted the angiogenesis of human umbilical vein endothelial cells. NINJ1 knockdown also promoted activation of the signal transducer and activator of transcription 3 (STAT3) signaling pathway in trophoblasts, and STAT3 inhibitor reversed NINJ1 knockdown-induced effects on trophoblast behaviors. Furthermore, pregnancy loss was attenuated by NINJ1 inhibition. Conclusion: NINJ1 contributes to the development of SA and triggers trophoblast cell dysfunction through inhibiting the STAT3 pathway.

Effect of High Dietary Carbohydrate on the Growth Performance, Blood Chemistry, Hepatic Enzyme Activities and Growth Hormone Gene Expression of Wuchang Bream (Megalobrama amblycephala) at Two Temperatures

Zhou, Chuanpeng,Ge, Xianping,Liu, Bo,Xie, Jun,Chen, Ruli,Ren, Mingchun Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.2

The effects of high carbohydrate diet on growth, serum physiological response, and hepatic heat shock protein 70 expression in Wuchang bream were determined at $25^{\circ}C$ and $30^{\circ}C$. At each temperature, the fish fed the control diet (31% CHO) had significantly higher weight gain, specific growth rate, protein efficiency ratio and hepatic glucose-6-phosphatase activities, lower feed conversion ratio and hepatosomatic index (HSI), whole crude lipid, serum glucose, hepatic glucokinase (GK) activity than those fed the high-carbohydrate diet (47% CHO) (p<0.05). The fish reared at $25^{\circ}C$ had significantly higher whole body crude protein and ash, serum cholesterol and triglyceride, hepatic G-6-Pase activity, lower glycogen content and relative levels of hepatic growth hormone (GH) gene expression than those reared at $30^{\circ}C$ (p<0.05). Significant interaction between temperature and diet was found for HSI, condition factor, hepatic GK activity and the relative levels of hepatic GH gene expression (p<0.05).

Theoretical study of SF6 decomposition products adsorption on metal oxide cluster-modified single-layer graphene

Qingfang Zhang,Yingang Gui,Han Qiao,Xianping Chen,Lingzhi Cao 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.105 No.-

GIS plays an irreplaceable role in the modern electrical system. However, partial discharge will inevitablyoccur under insulation defect conditions and may lead to serious insulation malfunction. The online monitoringmethod based on gas sensor is a feasible method to diagnose the severity of partial discharge inGIS. In this paper, metal oxide (TiO2, Fe2O3, NiO) cluster-modified single-layer graphene was proposed asa novel gas sensor to detect the characteristic components of SF6 decomposition products, SOF2 andSO2F2. Density functional theory calculations were carried out to study the gas adsorption and sensingmechanisms. The adsorption structures of gas molecules and the metal oxide cluster-modified singlelayergraphene were built and optimized. Then, the most stable structure was selected to analyze the correspondingadsorption parameters. Calculation results showed that metal oxides decoration reduces theenergy gap, improving the electrical conductivity and enhancing the adsorption activity of the graphenesurface. According to DOS and CDD analyses, TiO2 modification obtained the best adsorption effect. Calculation results show that the metal-oxide-modified graphene sensor provides an effective methodfor effectively estimating the operating state of GIS by detecting SF6 decomposition products.

Gas-sensing properties of Ptn-doped WSe2 to SF6 decomposition products

Lingna Xu,Yingang Gui,Wenjun Li,Qiaomei Li,Xianping Chen 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.97 No.-

Two-dimensional transition metal disulfides (TMDs) has attracted considerable attention due to itsexcellent physicochemical properties. In order to detect the SF6 decomposition products, transition metalPt cluster doping was chosen to enhance the adsorption property of intrinsic WSe2 monolayer. Theadsorption of SO2, SOF2 and SO2F2 on Ptn (n = 1–3) doped WSe2 monolayer is studied based on thefirstprinciplecalculation. The adsorption energy, charge transfer, and density of states of the interactionbetween the target gas molecules and Ptn-WSe2 were studied. The calculation results showed that Pt3doping dramatically enhances the adsorption of WSe2 to SO2 and SOF2 and SO2F2 molecules. Meanwhile,electrons transfer from Ptn-WSe2 surface to these three kinds of target gas molecules, reducing theconductivity of the adsorption system in different degrees. The results of this study not only haveimportant significance for explaining the sensing mechanism of Ptn-WSe2 adsorption to SF6 gasdecomposition products, but also provide a potential material for further development of gas sensingsensors.

Adsorption behavior of metal oxides (CuO, NiO, Ag2O) modified GeSe monolayer towards dissolved gases (CO, CH4, C2H2, C2H4) in transformer oil

Yingang Gui,Zhicheng Liu,Chang Ji,Lingna Xu,Xianping Chen 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.112 No.-

In this study, the adsorption behavior of CuO-GeSe, NiO-GeSe, and Ag2O-GeSe towards the main dissolvedgases CO, CH4, C2H2, and C2H4 in transformer oil was systematically studied based on DFT. The adsorptionstructure, band structure, density of states, deformation charge density, and molecular orbit were analyzedto explore the interaction between the modified monolayers and gases. The results show thatthe adsorption of the four target gases on pristine GeSe belongs to weak physical adsorption. Metal oxidesmodification improves the conductivity of GeSe, and the formation of numerous triangular structuresmakes the modified substrates hard to deform during gas adsorption. Due to the poor gas sensitivityand extremely short recovery time of CH4 on CuO-GeSe, NiO-GeSe, and Ag2O-GeSe, these three modifiedstructures are not suitable to be used as a material for detecting CH4. The conductivity of MO-GeSechanges in different degrees after adsorbing CO, C2H2, and C2H4. The gases can be detected accordingto the different change rule of conductivity upon adsorption. In addition, gas desorption from the substratecan be achieved by controlling the temperature. This study provides a theoretical basis for theapplication of gas sensors used in DGA.