Blocking of Cell Proliferation, Cytokines Production and Genes Expression Following Administration of Cordyceps Sinensis in the Bronchoalveolar Lavage Fluids Cells

Kuo, Yuh-Chi,Wu, Chia-Lin,Tasi, Wei-Jern,Wang, Jir-Yenn,Chang, Shi-Chung,Lin, Ching-Yuang,Shiao, Ming-Shi 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Yuh-Chi Kuo¹,Chia-Lin Wn¹,Wei-jern Tasi¹,Jir-Yenn Wang²,Shi-Chung Chang₃,Ching-Yung Lin⁴,and Ming-Shi Shiao⁴¹National Research Institute of Chinese Medicine, ²Department of Biology,Fu Jen University, ³Department of Chest, ⁴Department of Pediatrics, □De[art,amt of Medical Research and Education. Taipei Veterans General Hospital, Taipei, Taiwan Yuh-Chi Kuo¹,Chia-Lin Wn¹,Wei-jern Tasi¹,Jir-Yenn Wang²,Shi-Chung Chang²,Ching-Yung Lin³,and Ming-Shi Shiao⁴.¹National Research Institute of Chinese Medicine, ²Department of Biology,Fu Jen University, ³Department of Chest, ⁴Department of Pediatrics, □De[art,amt of Medical Research and Education. Taipei Veterans General Hospital, Taipei, Taiwan. Blocking of Cell Proliferation, Cytokines Production and Genes Expression Following Administration of Cordyceps sinensis in the Bronchoalveolar Lavage Fluids Cells. Proceedings of international Symposium on East-West Medicine, Seoul. 44-64, 1999.-Cordyceps sinensis is a major parasitic fungus on larva of Lepidoptera. It is one of the well know fungi used in traditional Chinese medicine for treatment asthma, lung and kideney diseases. Many fungi belonging to the genus Cordyceps have been demonstrated to produce natural products with various biological activities. Reported biological activities of genus cordyceps include:(a)inhibition of DNA and RNA synthesis;(b)enhancement of cell differentiation;(c)restruction of cytoskeleton;(d)inhibition of protein kinase activity;(e)antitumor activity on bladder, colon, lung carcinoma as well as fibroblastoma;(f)inhibition of the infection and revers transciptase activity of human immunodeficiency virus type 1; and(g) inhibition of methylation of nucleic acid. Thus, it is suggested that the extracts of Cordyceps sp. Contain antitumor, antiviral, and many other biological activities. In our previous studies, we proved that C. sinensis contains the antitumor and immunomodulatory agents. In the present studies, effects of C. sinensis on bronchoalveolar lavage fluids(BAL)cells were demonstrated. The crude methanolic extracts of C. sinensis were fractionated into 15 fractions by silica gel column chromatography. Effects of various fraction on BAL cells proliferation and lipopolysaccharide(LPS) stimulated interleukin-1β(IL-8),platelet activating factor(PAF), interleukin-6(IL-6), tumor necrosis factor-α(TNF-α),and interleukin-8(IL-8) production on BAL cells were determined. The result indicated that the CS-19-22 fraction suppressed BAL cells proliferation activated by LPS. The median inhibitory concentration(IC50) was 6㎍/ml. The CS-19-22 fraction also decreased IL-1β,PAF,IL-6, TNF-αand IL-8 production.The results of revers transcription-polymerase chain reaction(RT-PCR) demonstrated that the CS-19-22 fraction did not affect IL-1β,PAF,IL-6, TNF-α,and IL-8 mRNAs expression in BAL cells activated by LPS. By contrast, it inhibited the IL-10 mRNA expression but enhanced IFN-γand IL-12 mRNAs expression in activated BAL cells. Moreover, the CS-19-22 fraction blocked PAF-induced platelet aggregation. It is unlikely that cytotoxicity was involved. Because no cell deths were observable. We hypothesize that inhibitory mechanisms of CS-19-22 on BAL cells proliferation may be related to the impairments of gene expression and production of cytokines in BAL cells. The explanation of C. sinensis antiasthma function may involved the following(1)C.sinenesis contained immunomodulatory agents that stimulated IFN-γand IL-12 expression in TH1cells.(2)Enhanced secretion of IFN-γand IL-12 will inhibit the Tγcells immune responses Ex. Decreasing of IL-6 and IL-10 production.(3) The impairments of cytokines production in TH2 cells suppressed th BAL cells proliferation then more cytokines production Ex.IL-1β,PAF,IL-6,TNF-αand IL-8 in activated BAL cells were decreased. (4)The suppression of TH2cells immune responses will inhibit the growth and differentiation of B cells then IgE production will be decreased. (5)Reduced production of IgE will decreased asthma attack occurring in individuals. However, the results of the present study suggested that C. sinensis may also have acted to treat asthma in part by inhibiting BAL cells proliferation and cytokine gene expression and production. Future, experiments with treatment of animals with asthma with C. sinensis will be necessary to defined whether C. sinensis can reduce asthma injury. Plans are underway for the isolation of pure principal growth inhibitors and their mechanisms of action are subjected for further study.

Identification of novel rheumatoid arthritis-associated MiRNA-204-5p from plasma exosomes

Wu Long-Fei,Zhang Qin,Mo Xing-Bo,Lin Jun,Wu Yang-Lin,Lu Xin,He Pei,Wu Jian,Guo Yu-Fan,Wang Ming-Jun,Ren Wen-Yan,Deng Hong-Wen,Lei Shu-Feng,Deng Fei-Yan 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Rheumatoid arthritis (RA) is an autoimmune disease characterized by infiltration of immune cells in the synovium. However, the crosstalk of immune cells and synovial fibroblasts is still largely unknown. Here, global miRNA screening in plasma exosomes was carried out with a custom microarray (RA patients vs. healthy controls = 9:9). A total of 14 exosomal miRNAs were abnormally expressed in the RA patients. Then, downregulated expression of exosomal miR-204-5p was confirmed in both the replication (RA patients vs. healthy controls = 30:30) and validation groups (RA patients vs. healthy controls = 56:60). Similar to the findings obtained in humans, a decreased abundance of exosomal miR-204-5p was observed in mice with collagen-induced arthritis (CIA). Furthermore, Spearman correlation analysis indicated that plasma exosomal miR-204-5p expression was inversely correlated with disease parameters of RA patients, such as rheumatoid factor, erythrocyte sedimentation rate, and C-reactive protein. In vitro, our data showed that human T lymphocytes released exosomes containing large amounts of miR-204-5p, which can be transferred into synovial fibroblasts, inhibiting cell proliferation. Overexpression of miR-204-5p in synovial fibroblasts suppressed synovial fibroblast activation by targeting genes related to cell proliferation and invasion. In vivo assays found that administration of lentiviruses expressing miR-204-5p markedly alleviated the disease progression of the mice with CIA. Collectively, this study identified a novel RA-associated plasma exosomal miRNA-204-5p that mediates the communication between immune cells and synovial fibroblasts and can be used as a potential biomarker for RA diagnosis and treatment.

일반논문 : 抗戰時期吳鼎昌與州的經濟社會發展

임서무 ( Xu Wu Lin ) 東亞大學校附設 石堂傳統文化硏究院 2011 石堂論叢 Vol.0 No.51

항전시기 오정창은 귀주성 정부 주석직을 7년간 맡아 보면서 다양한 정책을 통해 귀주의 발전을 촉진하였다. 위생 보건, 금연, 군사훈련, 그리고 체육 등의 분야에 많은 노력을 기울였으며, 인구의 증가와 의식 수준의 향상을 위해 노력하였다. 농업과 임업 목축업과 양잠업, 전기 및 광공업, 교통과 운수 등의 사업을 통해 귀주기업주식회사를 설하여 공업화의 길을 모색하였다. 현장(縣長)의 선발 과정과 조직 정비, 현 정부와 향진 간의 권리와 책임을 구분하여 기층의 민주 정치를 발전시켰다. 이러한 시책은 남경 국민정부의 통치를 옹호하는 것이었으며, 또한 귀주의 현대화를 추진함으로써 후방을 튼튼히 하여 항전의 승리에 일정 정도 공헌함으로써 새로운 정치학 체계의 정치적 주장과 역사적 가치를 잘 보여주고 있다. Wu Ding-chang had been undertaken the government chairman in Guizhou province for seven years during the Anti-Japanese War. He took some policies of sanitation, anti-smoking, military training and physical education to increase the population. At the same time, he also used methods of school education and administration training to enhance population`s quality. And he initiated a new path of industrialization by developing agriculture, forestry, animal husbandry, raising silk worms, electricity, industry, mining and transportation and setting up Guizhou enterprise stock corporation. Moreover, he improved the methods of choosing the head of a county and ranks building, divided the rights and responsibilities between county and small towns and developed skeleton democratic politics. These policies impelled the economic and social modernization in Guizhou and made great contributions for consolidating Kuomintang-controlled areas and supporting the victory during the Anti-Japanese War.

Evolution of Pharmacy Education in Taiwan

Fe-Lin Lin Wu,Li-Jiuan Shen,Karin C,S,Chen Liu 대한약학회 2008 大韓藥學會 總會 및 學術大會 Vol.2008 No.1

INVESTIGATING THE DEVELOPMENT OF BRAND LOYALTY IN BRAND COMMUNITIES FROM A POSITIVE PSYCHOLOGY PERSPECTIVE

Chia-Wu Lin,Kai-Yu Wang,Shu-Hao Chang,Jin-An Lin 글로벌지식마케팅경영학회 2016 Global Marketing Conference Vol.2016 No.7

Brand communities have been increasingly used by marketers to build brands. A brand community can be defined as a “…group of consumers with a shared enthusiasm for the brand and a well-developed social identity, whose members engage jointly in group actions to accomplish collective goals and/or express mutual sentiments and commitments” (Bagozzi and Dholakia, 2006, p. 45). Recent research on brand communities has begun to identify the importance of consumers’ psychological processes in regard to developing successful brand communities. Based on the flow theory from positive psychology, we propose that flow could be generated by brand community characteristics and plays an important role in influencing brand community members’ attitudes toward the brand. Specifically, we propose a model that identifies brand community characteristics (i.e., community cohesiveness and information quality) that produce flow experience and how the flow experience impacts brand identification and brand loyalty. Members from 31 automobile brand communities participated in this survey study, and 580 validated questionnaires were returned. Structural Equation Model was used to test the research hypotheses. The results show that community cohesiveness and information quality positively directly influence brand identification and also indirectly influence brand identification via flow. Flow also positively influences members’ brand identification and, subsequently, impacts brand loyalty. The results from our research contribute to the branding, brand community, and flow theory literature.

Evaluation of Clinical Training Program for Nurse Diabetes Educators in Taiwan

Yueh-Chu Wu,Hsiu-Li Wu,Pei-Yung Liao,Shih-Li Su,Su-Lan Lin,Shu-Man Lin,Hui-Fang Wang,Ching-Wen Wu,Shi-Dou Lin,Hon-Ke Sia 한국간호과학회 2009 한국간호과학회 학술대회 Vol.2009 No.10

High-Frequency Isolated Medium-Voltage AC Grid-connected PV Power Generation System Structure based on DC Bus Collection

Lin. Lin,Qi. Chen,Xingang. Wu,Chuang. Liu,Haoran. Zhang,Dongfeng. Yang 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5

This paper proposes an isolated mediumvoltage AC grid-connected PV power generation system structure based on DC bus collection with high-frequency link concept. The proposed system structure consists of centralized photovoltaic power plant, inverter unit based on isolated bridge converter (I-BC), and capacitor at the junction of dc bus. PV plant is divided into many arrays, each PV array is connected in parallel with each phase inverter unit. Inverter unit is composed of two I-BC. Compare with conventional two-stage PV inverter, I-BC can totally eliminate the individual DC-link capacitors at the highvoltage side, which leads to extremely reduced system volume and voltage-balancing control between individual capacitors. Second harmonic power oscillations seen in typical system structure can also be eliminated or reduced through use of the proposed system. This paper presents the analysis and operation of the proposed PV system structure with experimental results on a scaled-down laboratory.

MEROMORPHIC FUNCTIONS PARTIALLY SHARED VALUES WITH THEIR SHIFTS

Lin, Weichuan,Lin, Xiuqing,Wu, Aidi Korean Mathematical Society 2018 대한수학회보 Vol.55 No.2

We prove some uniqueness theorems of nonconstant meromorphic functions partially sharing values with their shifts. As an application, we obtain a sufficient condition on periodic meromorphic functions. Moreover, some examples are given to illustrate that the conditions are sharp and necessary.

Influence of Clay on Selected Properties of Wood Fiber HDPE Composites

( Wu Qing Lin ),( Yong Lei ),( Craig M. Clemons ),( Sun Young Lee ) 韓國木材工學會 2006 한국목재공학회 학술발표논문집 Vol.2006 No.-

THE EMPIRICAL INVESTIGATION OF LONG - RUN PURCHASING POWER PARITY : THE CASE OF TAIWAN EXCHANGE RATES

WU, JYH LIN 한국국제경제학회 1996 International Economic Journal Vol.10 No.4