http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Anticancer effect of silibinin on the xenograft model using MDA-MB-468 breast cancer cells
Won Ho Kil,Sang Min Kim,Jeong Eon Lee,Kyoung Sik Park,Seok Jin Nam 대한외과학회 2014 Annals of Surgical Treatment and Research(ASRT) Vol.87 No.4
Purpose: The aim of this study is to know whether silibinin has an anticancer effect on triple negative breast cancer xenograft model using MDA-MB-468 cells. Methods: To establish the xenograft model, we injected the MDA-MB-468 cells into female Balb/c-nude mice. After establishing a xenograft model, oral silibinin was administered to the tested mice in the way of 200 mg/kg for 45 days. The difference of mean tumor volume between silibinin fed mice and control mice was analyzed. The epidermal growth factor receptor (EGFR) phosphorylation in MDA-MB-468 cells was analyzed by Western blotting. The expression of VEGF, COX-2, and MMP-9 genes in tumor tissue was analyzed by real-time polymerase chain reaction (PCR). Results: In the xenograft model using MDA-MB-468 cells, we found that oral administration of silibinin significantly suppressed the tumor volume (silibinin treated mice vs. control mice; 230.3 ± 61.6 mm3 vs. 435.7 ± 93.5 mm3, P < 0.001). The phosphorylation of EGFR in MDA-MB-468 cells was inhibited by treatment with 50 mg/mL of silibinin. In real time-PCR analysis of tumor tissue obtained from sacrificed mice, the gene expression of MMP-9, VEGF, and COX-2 was 51.8%?80% smaller in silibinin group than that of control group and we can also verify the similar result using Western blotting analysis. Conclusion: We verified that silibinin had anticancer effect on xenograft model of MDA-MB-468 cells in the way of preventing the phosphorylation of EGFR and eventually suppressed the production of COX-2, VEGF, and MMP-9 expression. Finally, the tumor volume of xenograft models was decreased after administration of Silibinin.
A Review of Discarded Organs from Deceased Donors in South Korea
( Kil Hwan Kim ),( Youngrok Choi ),( Ho-seong Han ),( Yoo-seok Yoon ),( Jai Young Cho ),( Sungho Kim ),( In Gun Hyun ),( Won Hyun Cho ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1
Aims: Despite a steady increase in the number of organ transplantations performed each year throughout the world, the shortage of available organs for transplantation is worsening. This study aims to analyze the current status of discarded organs (Harvested, but not be transplanted organ) from deceased donors in Korea. Methods: We used KODA (Korea Organ Donation Agency) and KONOS (Korean Network for Organ Sharing) database to search for deceased donor organ transplantations (DDOTs) performed between 2013 and 2016. Based on the data, we analyzed the incidence and causes of discarded organs according to organs. Results: In South Korea, 6315 DDOTs were performed between 2013 and 2016. A total of 63 discarded organs were procured. The most common organ was the kidney (n=24) followed by islet cell (n=23), lung (n=9), liver (n=6) and pancreas (n=1). There were no discarded hearts. Except for islet cells, the most common reasons for organs to be discarded were organ dysfunction and vascular defects. All of discarded islet cells (n=23) were caused by separation failure or shortage of proper cell number. We also analyzed cases of discontinuation of harvesting operations for past 4 years. Out of 39 total cases, donor’s poor organ condition (n=27) was the most prevalent reason for discontinuation. Conclusions: It is obvious that the shortage of organ donors will continue to get worse. To prevent and reduce unnecessary procurement and discard rate, we should more carefully perform preoperative evaluations and intraoperative inspection. Also, it would be necessary to implement a better control system.
( Won Seok Kang ),( Joon Seok Lim ),( Jae Hee Cheon ),( Yong Chan Lee ),( Won Ho Kim ),( Sang Kil Lee ) 대한소화기학회 2007 SIDDS Vol.9 No.-
Background/Aims: Stomach cancer is the most common from of cancer, and is the second leading cause of cancer related death in Korea. 3.4% of stomach cancer patients have synchronous of metachronous cancer with colorectal cancer as the most common type (0.6%). Patients with stomach cancer might carry a high risk for colorectal cancer that requires surveillance colonoscopy. CT colonography (CTC) provides for effective and relatively noninvasive risk stratification as a method for colon cancer screening. This study aims at determining the usefulness of CTC as a postoperative follow-up surveillance study after stomach cancer surgery. Methods: From May 2006 to April 2007, contrast-enhanced CTC was performed in Yonsei University severance Hospital for detection of recurrent lesions in 410 consecutive patients (275 male, 135 female) who have had surgery for stomach cancer CTC interpretation was performed with primary three-dimensional polyp detection. Patients with polyp(s) measuring 6mm or larger were referred for optical colonoscopy (OC). Results: Of 410 stomach cancer postoperative patients, recurrence of stomach cancer was not noted and 45 colorectal polyp(s) were detected on CTC in 36 (8.8%) patients. OC was recommended in these patients and concordant lesions were identified in 27 (60.0%) of 45 polyps. Eighty-six polyps were detected on OC in 31 patients, and no polyps were detected on OC in 5 patients. Four colorectal carcinomas were detected in 4 patients CTC. Sensitivity and positive predicitive value of CTC for polyp detection were 60.0% and 62.8%, respectively. Total 46 polyps were missed on CTC. The most common site of missed polyps was sigmoid colon. Eighteen (39.1%) were larger than 6 mm and 1 carcinoma was included in the missed polyps. Conclusion: For patients who have undergone surgical treatment for stomach cancer, CTC is clinically useful as their follow-up surveillance study. However, more efforts are needed to find the ways to improve the low sensitivity of CTC.