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- 저자 : Wensong Xu (검색결과 5 건)
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( Wensong Jin ),( Hui Lin ),( Huifang Gao ),( Zewang Guo ),( Jiahuan Li ),( Quanming Xu ),( Shujing Sun ),( Kaihui Hu ),( Jung-kul Lee ),( Liaoyuan Zhang ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.4
N-acyl-homoserine lactone quorum sensing (AHL-QS) has been shown to regulate many physiological behaviors in Serratia marcescens MG1. In the current study, the effects of AHL-QS on the biosynthesis of acid and neutral products by S. marcescens MG1 and its isogenic ΔswrI with or without supplementing exogenous N-hexanoyl-L-homoserine lactone (C<sub>6</sub>-HSL) were systematically investigated. The results showed that swrI disruption resulted in rapid pH drops from 7.0 to 4.8, which could be restored to wild type by supplementing C<sub>6</sub>-HSL. Furthermore, fermentation product analysis indicated that ΔswrI could lead to obvious accumulation for acidogenesis products such as lactic acid and succinic acid, especially excess acetic acid (2.27 g/l) produced at the early stage of fermentation, whereas solventogenesis products by ΔswrI appeared to noticeably decrease by an approximate 30% for acetoin during 32-48 h and by an approximate 20% for 2,3-butanediol during 24-40 h, when compared to those by wild type. Interestingly, the excess acetic acid produced could be removed in an AHL-QS-independent manner. Subsequently, quantitative real-time PCR was used to determine the mRNA expression levels of genes responsible for acidogenesis and solventogenesis and showed consistent results with those of product synthesis. Finally, by close examination of promoter regions of the analyzed genes, four putative luxI box-like motifs were found upstream of genes encoding acetyl-CoA synthase, lactate dehydrogenase, α-acetolactate decarboxylase, and Lys-like regulator. The information from this study provides a novel insight into the roles played by AHL-QS in switching from acidogenesis to solventogenesis in S. marcescens MG1.
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The role of microenvironment in aggregation of the 293-human embryonic Kidney cells
Wensong Tan,Liang Zhao,Li Fan,Xu Zhang,Minglong Zhu 한국화학공학회 2007 Korean Journal of Chemical Engineering Vol.24 No.5
cells. Key factors such as calcium, magnesium and shear stres were investigated in detail and cell aggregate size con-trol was applied to facilitate cell retention improvement. It was found that the concentration of calcium ion affectedthe aggregation of 293-HEK cells drastically and exhibited direct proportion to the average diameter of 293-HEK cellaggregates. Similar effect was also discovered in magnesium but to a lower extend. Results also showed the growthof 293-HEK cells was influenced when the concentrations of calcium or magnesium ions were below 0.1 mol/L,and that was more significant with magnesium. Furthermore, aggregation as well as growth was affected by hard in-trolled to proper size as anticipated and the average sedimentation speed of agregated cells increased about 20 timesto single cells. This is highly advantageous to cell retention improvement either in perfusion cultures or media exchangebefore adenovirus infection by proper control of the cell aggregate size, and thus a high cell concentration and ad-enovirus production potentially can be achieved.
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Wensong Ma,Ligang Lin,Jing Yang,Zitian Liu,Xinyang Li,Meina Xu,Xiaopeng Li,Chunhong Wang,Qingping Xin,Kongyin Zhao 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.120 No.-
Nowadays, the use of separation membranes to deal with oil–water emulsions has gained popularity. However, oil fouling of membrane surfaces during the separation of oil–water emulsion is still a significantchallenge. In this study, inspired by the biological coral stone structure, the gel layer was firmlyattached to the surface of membrane using a simple co-blending and cross-linking strategy. A superwettingmembrane (PVDF/CD-SA) with a coral stone structure was obtained. The PVDF/CD-SA membranehad a high permeate flux that was 4.2 times higher than that of the original membrane and a high separationefficiency of about 99.2 % for the separation of oil–water emulsion. Furthermore, the membranehad outstanding chemical stability. The fluxes of several different oil-in-water emulsions significantlyimproved, and the separation efficiencies were as high as 98 %. Moreover, the separation efficienciesand contact angles of the membrane remained unchanged after numerous cycles of use. The membraneexhibited excellent superhydrophilicity in air (instantaneous water wetting in air) and superoleophobicityunder water (underwater oil contact angle > 156). Most importantly, the oil was able to automaticallydetach from the surface of membrane, resulting in self-cleaning performance. Therefore, this PVDF/CD-SAmembrane eliminated the problem of oil adhesion, exhibiting excellent potential for practical applicationsin oil–water separation.
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Experimental study of the association between sandstone size effect and strain rate effect
Siming Kao,Guang-Ming Zhao,Wensong Xu,Xiang Cheng,Chunliang Dong,Ruofei Zhang 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.9
The association between sandstone size effect and strain rate effect were investigated experimentally with a split Hopkinson pressure bar (SHPB) system. The sandstone samples with Φ50 mm and different lengths were loaded under the constant ratio of punch velocity to sample length to study their size effects. Sandstone samples with constant length of 25 mm were taken as the reference to study their strain rate effects. Results indicate that, under the same velocity of the punch, strain rate of each sandstone sample is inversely proportional sample length; dynamic strength of sandstone increases with the strain rate and the length to diameter ratio (L/D), and presents a quadratic curvilinear relation with strain rate while presenting a cubic curvilinear relation with sample L/D; the reasonable L/D of Φ50 mm sandstone samples ranges from 0.5 to 0.8; that dissipated energy can present a fixed proportional relation with punch kinetic energy is unrelated to sample length.
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Xinyang Li,Ligang Lin,Zitian Li,Jing Yang,Wensong Ma,Xu Yang,Xiaopeng Li,Chunhong Wang,Qingping Xin,Kongyin Zhao 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.119 No.-
In this study, inspired by ‘‘micro-explosion” strategies, a separation membrane with high porosity andpermeability, and highly efficient separation performance was prepared. With the use of polyvinylidenefluoride (Solvay6015) as membrane material, azodicarbonamide (AC) as an ‘‘explosion center point” tothe casting membrane solution, and NaOH as an ‘‘external stimulus” in the coagulation bath, the twochemicals undergo in-situ foaming reaction to form a loose nanofiltration membrane. FTIR, XPS, andTGA results demonstrated that the decomposition of AC was complete, which produced gases thatincreased the porosity of the membrane. The optimized membrane has a higher flux(101.72 L m2 h1 at 0.3 Mpa), higher negative surface charge, and better mechanical properties underthe premise of separating CR/NaCl. In the separation of pollutants with different molecular weights,the permeation flux of the optimized membrane increased by more than double. This foaming technologywas also applied to another membrane material, ethylene vinyl alcohol, from which we found that themembrane also had higher porosity and better permeability. Together, this paper presents an in-situfoaming method for preparing separation membranes and lays the foundation for solving the trade-offbetween membrane permeability and rejection in dye/salt separation.
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