The Effect of the Major Components of Fructus Cnidii on Osteoblasts In Vitro

Wenping Zhang,Dongming Ma,Qiduo Zhao,Torao Ishida 사단법인약침학회 2010 Journal of Acupuncture & Meridian Studies Vol.3 No.1

In traditional Chinese medicine, the cause of weak bones or bone loss is generally regarded as a result of kidney deficiency. Fructus Cnidii (FC), which is also known as She-Chuang-Zi, is a traditional herb that has been claimed to have kidney warming effects that invigorate Yang. In this study, we tried to determine the bone production-inducing effect of FC on osteoblastic cells in vitro using osthole, the main component of FC. Osteoblasts were isolated from neonatal Sprague-Dawley rat calvaria using the tissue piece culture method and treated with various concentrations of osthole ranging from 2.5 to 640 μg/mL, together with a blank control. Cell proliferation,alkaline phosphatase (ALP) activity, and bone nodules were measured. The cells were examined by hematoxylin-eosin staining, the Gomori Calcium-Cobalt method and immunofluorescent staining. The 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide (or MTT) assay, ALP assay, and bone nodule results indicated significantly enhanced osteoblastic proliferation and differentiation at concentrations of osthole ranging from 40 to 320 μg/mL. Concentrations lower than 40 μg/mL seemed less effective, and cytotoxicity to osteoblasts was observed at concentrations higher than 320 μg/mL. These results indicate that osthole is effective at inducing osteoblastic bone formation through the up-regulation of ALP activity. FC is a Chinese herb used to treat lumbar pain in clinical practice. Further studies concerning the effects and mechanism of osthole on osteoporotic patients and animals should be performed, as these studies may lead to the development of a drug treatment for osteoporosis in the future.

A Pilot Study Exploring the Effects of Reflexology on Cold Intolerance

Wenping Zhang,Shougo Takahashi,Takashi Miki,Hisayo Fujieda,Torao Ishida 사단법인약침학회 2010 Journal of Acupuncture & Meridian Studies Vol.3 No.1

Cold intolerance is an inability to tolerate cold temperatures and is accompanied by symptoms including headache, shoulder discomfort, dizziness and palpitations. The current study was performed to examine whether reflexology therapy affected cold intolerance in human subjects and whether the treatment was systemically effective. Ten female volunteer examinees with subjective feelings of cold were examined. After a 5-minute foot bath, 10 minutes of reflexology therapy was performed on their left foot. Skin temperature and blood flow were estimated before and after treatment, together with an interview concerning their feelings of cold and daily habits. In addition, how the recovery rate was affected by the application of a chilled-water load was also estimated. Along with significant increases in skin temperature and blood flow compared with pre-treatment at the bilateral points of KI-1, LR-3, and BL-60, a faster recovery after the application of the chilled-water load was also seen in the lower limbs on both sides. From these results, we conclude that reflexology has systemic effects and is an alternative method for treating cold intolerance.

Influence of the silencing sex-peptide receptor on Bactrocera dorsalis adults and offspring by feeding with ds-spr

Wenping Zheng,Yaru Liu,Weiwei Zheng,Yunli Xiao,Hongyu Zhang 한국응용곤충학회 2015 Journal of Asia-Pacific Entomology Vol.18 No.3

RNA interference (RNAi) has been shown to control insect pests using plant dsRNA expression. A key question in potentially applying RNAi is the possible effects on insects after being fed with dsRNA produced by a transgenic plant. Sex peptide receptor (spr) is the key gene that regulates the suppression of female receptivity and induction of oviposition. In this study, the expression level of the spr genewas significantly down-regulated to 52% by feeding Bactrocera dorsalis adults with ds-spr. The RNAi effects of continuous feeding ds-spr to adults led to a highly mortality rate, decreased their egg production capacity and profoundly impacted the eclosion rate of their offspring. Our results demonstrate that RNAi through uninterrupted dsRNA feeding can be used as a strategy to control insect pests. Moreover, the research presented here provides a potential RNAi target gene for controlling B. dorsalis and a theoretical basis for universally applying RNAi in insect pest management.

Choosing Knots by Quadratic Polynomial

Zhang Caiming,Wang Wenping (사)한국CDE학회 2007 한국CAD/CAM학회 국제학술발표 논문집 Vol.2007 No.8

Joint Estimation of Vehicle State and Parameter Based on Maximum Correntropy Adaptive Unscented Kalman Filter

Zhang Feng,Feng Jingan,Qi Dengliang,Liu Ya,Shao Wenping,Qi Jiaao,Lin Yuangang 한국자동차공학회 2023 International journal of automotive technology Vol.24 No.6

To address the problem of poor robustness and accuracy of vehicle state and parameter estimation by conventional Kalman filter in the non-Gaussian environments, a three-degree-of-freedom vehicle model with an improved Dugoff tire model is established and a joint estimator of vehicle state and parameter is designed using the Maximum Correntropy (MC) adaptive unscented Kalman filter (AUKF) algorithm in order to simultaneously estimate and identify the yaw rate, longitudinal vehicle speed, lateral vehicle speed, vehicle mass and rotational inertia. The proposed joint estimator algorithm was validated by Simulink/CarSim simulation testbed under Double Lane Change and Sine Wave Steering Input conditions. The results show that MC combined with AUKF (MCAUKF) algorithm has higher estimation accuracy and better convergence compared to the unscented Kalman filter (UKF) and the MC combined with UKF (MCUKF) in non-Gaussian environments, and the MCAUKF estimator is more suitable for state estimation and parameter identification of real vehicles.

Aerodynamic Design of a Novel Low-Reynolds-Number Airfoil for Near Space Propellers

Zhang, Shunlei,Yang, Xudong,Song, Bifeng,Song, Wenping The Society for Aerospace System Engineering 2015 International Journal of Aerospace System Engineer Vol.2 No.1

For improving the efficiency of near space propellers working over 20km, performances of their streamwise sections, i.e. low-Reynolds-number airfoils which work at $10^4-10^5$ Reynolds numbers, are significant. Based on the low-Reynolds-number CFD technology, this paper designs a novel low-Reynolds-number airfoil. Unsteady characteristics of the laminar separation bubble on novel airfoil and a typical conventional airfoil are studied numerically, and the Reynolds number effect is investigated. Results show that at $10^4-10^5$ Reynolds numbers, unsteady aerodynamic characteristics of the novel airfoil are severely weakened and its lift-to-drag ratio can increase about 100%.

Synthesis of Uniform Polyaniline Nanosheets and Nanotubes: Dependence of Morphology on the pH

Yumei Zhang,Chengfu Dou,Wenping Wang,Qiang Wang,Ningchuan Feng 한국고분자학회 2016 Macromolecular Research Vol.24 No.8

Polyaniline (PANI) nanomaterials were synthesized by simplified template-free method with ammonium persulfate (APS) as an oxidant. The strong dependence of morphology of polyaniline on the initial pH of the reaction solution has been found. PANI morphology transformed from nanosheets to nanotubes with the decreasing initial pH from 6.70~6.50 to 5.30~5.00, and nanotubes changed further to irregular granules with the decreasing pH lower than 2.26. Polyaniline nanosheets were highly crystalline (2θ=6.2°) but poor in conductivity (5.7×10-5 S/cm) and electroactivity due to low doping level. Though polyaniline nanotubes were poorly crystalline, it had a relative high conductivity (3.4×10-2 S/cm) and maintained the electroactivity in acidic and neutral media which had potential applications in biomedical fields such as tissue engineering and biosensor.

Design and Control of Bi-directional DC/DC converter for 30kW fuel cell power system

Xiao Li,Wenping Zhang,Haijin Li,Ren Xie,Dehong Xu 전력전자학회 2011 ICPE(ISPE)논문집 Vol.2011 No.5

Fuel cell (FC) power system is considered as an alternative energy power generation system in the future. This paper studies the bidirectional DC/DC converter for a 30㎾ three-phase fuel cell power system. The FC power system is sensitive to load characteristics in stand-alone mode, because rapid change or unbalance of the three-phase load will endanger the safety and expectancy of fuel cell. The bidirectional DC/DC converter with energy storage component is used to provide the dynamic power to avoid fast changing in fuel cell output. In addition, it acts as DC bus capacitors to absorb the low frequency current ripple. Therefore, fuel cell is separated from the low frequency current ripple and large DC bus electrolytic capacitors can be eliminated. Through investigating topology of the bidirectional DC/DC converter, three-level bidirectional DC/DC converter (TLBD) is used for the 30㎾ fuel cell power system. The design and control of the TL-DB is described. TL-DB with its control strategy is verified by the experimental results.

Research on 3/6-phase Winding Switching Integrated Starter Generator for Range Extender Electric Vehicles

Guo Yingzhi,Shi Liwei,Zhang Wenchao,Chai Wenping,Kwon Byung-il 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5

To improve the output current of an Integrated Starter Generator (ISG) with stable windings at high speed, a 3/6-phase winding switching permanent magnet (PM) machine is proposed this paper. At low-speed starting mode, large number of coils are series connected as conventional three-phase windings to keep the starting performance in starting the engine. At high-speed generating mode, the coils are connected as six-phase winding to generate greater current. Finite element analysis software was utilized to verify the EMF and output current performance of 3/6-phase winding switching of ISG with consequent-pole rotor, which was designed for Range Extender Electric Vehicles (REEV). The experimental results verified that the proposed ISG increased the output current by six-phase windings in generating mode.

FACILE SOLVOTHERMAL SYNTHESIS OF MESOSTRUCTURED CHITOSAN-COATED Fe 3 O 4 NANOPARTICLES AND ITS FURTHER MODIFICATION WITH FOLIC ACID FOR IMPROVING TARGETED DRUG DELIVERY

MAO SHEN,CHENGLIN WU,WENPING JIA,CHENGHONG LI,ZHILI ZHANG,YANGMIN JIN,GUODONG FAN,CAIPING LIN 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2014 NANO Vol.9 No.7

Mesostructured chitosan-coated Fe 3 O 4 nanoparticles (CS-coated Fe 3 O 4 NPs) were synthesizedby a facile one-step solvothermal method via using chitosan as a surface-modi¯cation agent. Subsequently, the surfaces of CS-coated Fe 3 O 4 NPs were successfully conjugated with folic acid(FA) molecules to obtain FA – CS-coated Fe 3 O 4 NPs for improving targeted drug delivery. Themorphology, chemical component and magnetic property of as-prepared composite nanoparticleswere characterized by Fourier transform infrared spectroscopy (FTIR), X-ray di®raction (XRD),dynamic light scattering (DLS), scanning transmission electron microscopy (SEM), transmissionelectron microscopy (TEM), thermal gravimetric analysis (TGA) and vibrating sample magne-tometer (VSM). Furthermore, doxorubicin hydrochloride (DOX) as a model drug was encap-sulated for investigating drug release pattern in vitro. The results show that the magnetizationsaturation value of FA – CS-coated Fe 3 O 4 NPs was about 28.5 emu/g, exhibiting super-paramagnetic properties and mesostructure. DOX could be loaded to FA – CS-coated Fe 3 O 4 NPswith high capacity about 27.9%, and the release rate of DOX could be adjusted by the pH value. This work demonstrates that the prepared magnetic nanoparticles have potential applications inthe treatment of cancer as targeting drug delivery carriers.