Molecular Cloning, Segmental Distribution and Ontogenetic Regulation of Cationic Amino Acid Transporter 2 in Pigs

Zou, Shi-geng,Zhi, Ai-min,Zhou, Xiang-yan,Zuo, Jian-jun,Zhang, Yan,Huang, Zhi-yi,Xu, Ping-Wen,Feng, Ding-yuan Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.5

The goal of this study was to elucidate the expression and segmental distribution of the glomerular cationic amino acid metabolism transporter-2 (CAT-2) and thus to improve our understanding of porcine cationic amino acid transporters and amino acid absorption. Porcine CAT-2 was cloned, sequenced and characterized. The predicted amino acid sequence of porcine CAT-2 shared 86.1% and 92.1% identity with human and mouse CAT-2A, respectively. The tissue distribution patterns and ontogenic changes of CAT-2 mRNAs were determined by real-time Q-PCR. The results showed that porcine CAT-2 was highly expressed in the heart and intestinal tract (duodenum, ileum and jejunum). In addition, the mRNA of CAT-2 was found in liver, lung, kidney, brain and muscle. Within the intestinal tract, CAT-2 mRNA was most abundant in the ileum and rarely expressed in the duodenum. In the duodenum, the levels of CAT-2 mRNA reached their peak on day 7 (p<0.05) while in the jejunum, levels were low on day 1 and 7 and increased rapidly after day 26 before peaking on days 30 and 60 (p<0.05). The levels then dramatically decreased by day 90 (p<0.05). In the ileum, levels achieved their maximum on day 30 and then decreased significantly on day 60 (p<0.05).

Modelling of vanadium redox flow battery performance and degradation dynamics

Wen-Jiang Zou(추문강),Seunghun Jung(정승훈) 대한기계학회 2023 대한기계학회 춘추학술대회 Vol.2023 No.6

In vitro disinfection efficacy and clinical protective effects of common disinfectants against acute hepatopancreatic necrosis disease (AHPND)- causing Vibrio isolates in Pacific white shrimp Penaeus vannamei

Zou Peizhuo,Yang Qian,Wang Hailiang,Xie Guosi,Cao Zhi,Chen Xing,Gao Wen,Huang Jie 한국미생물학회 2020 The journal of microbiology Vol.58 No.8

Acute hepatopancreatic necrosis disease (AHPND) is one of the most significant bacterial diseases in global shrimp culture, causing severe economic losses. In the present study, we carried out in vitro antimicrobial tests to investigate the disinfection efficacy of 14 common disinfectants toward different AHPND-causing Vibrio spp., including eight isolates of V. parahaemolyticus, four isolates of V. campbellii, and one isolate of V. owensii. Polyhexamethylene biguanidine hydrochloride (PHMB) was revealed to possess the strongest inhibitory activity. Through analyzing and evaluating the results of antimicrobial tests and acute toxicity test, we selected PHMB and hydrogen peroxide (H2O2) for further clinical protection test. Clinical manifestations indicated that both PHMB (2 mg/L and 4 mg/L) and H2O2 (12 mg/L) could effectively protect juvenile Penaeus vannamei from the infection of V. parahaemolyticus isolate Vp362 at 106 CFU/ml, and the survival rate was over 80%. When the bacterial concentration was reduced to 105 CFU/ml, 104 CFU/ml, and 103 CFU/ml, the survival rate after treated by 1 mg/L PHMB was 64.44%, 93.33%, and 100%, respectively. According to the results, PHMB and H2O2 showed a lower toxicity while a better protection activity, particularly against a lower concentration of the pathogens. Therefore, these two disinfectants are proved to be promising disinfectants that can be applied to prevent and control AHPND in shrimp culture. Moreover, the methods of this study also provided valuable information for the prevention of other important bacterial diseases and suggested a reliable means for screening potential drugs in aquaculture.

Comparison of the Effects of Propofol and Midazolam on Inflammation and Oxidase Stress in Children with Congenital Heart Disease Undergoing Cardiac Surgery

Wen-fang Xia,Qing-shan Zhou,Yu Liu,Qi-zhu Tang,Han-dong Zou 연세대학교의과대학 2011 Yonsei medical journal Vol.52 No.2

Purpose: To investigate and compare the effects of propofol and midazolam on inflammation and oxidase stress in children with congenital heart disease undergoing cardiac surgery. Materials and Methods: Thirty-two ASA class I-II children with congenital heart disease undergoing cardiac surgery were randomly divided into two groups: propofol combined with low dose fentanyl (PF group, n = 16) and midazolam combined with low dose fentanyl (MF group, n = 16). Tracheal extubation time and length of Intensive Care Unit (ICU) stay were recorded. Blood samples were taken before operation (T_0), at 2 h after release of the aorta cross-clamp (T_3) and at 24 h after operation (T_4) to measure interleukin 6 (IL-6), IL-8, superoxide dismutase (SOD) and malondialdehyde (MDA) levels. Myocardium samples were collected at 10-20 min after aorta cross-clamp (T_1) and at 10-20 min after the release of the aorta cross-clamp (T_2) to detect heme oxygenase-1 (HO-1) expression. Results: Tracheal extubation time and length of ICU stay in PF group were significantly shorter than those of the MF group (p < 0.05, respectively). After cardiopulmonary bypass, IL-6, IL-8 and MDA levels were significantly increased, and the SOD level was significantly reduced in both two groups, but PF group exhibited lower IL-6, IL-8 and MDA levels and higher SOD levels than the MF group (p < 0.05, respectively). The HO-1 expression in the PF group was significantly higher than that in MF group at the corresponding time points (p < 0.05, respectively). Conclusion: Propofol is superior to midazolam in reducing inflammation and oxidase stress and in improving post-operation recovery in children with congenital heart disease undergoing cardiac surgery.

The dynamic DEA assessment of the intertemporal efficiency and optimal quantity of patent for China’s high-tech industry

Wen-Jie Zou,Chin-Wei Huang,Yung-Ho Chiu,Neng Shen,Shu-Mei Wang 기술경영경제학회 2016 ASIAN JOURNAL OF TECHNOLOGY INNOVATION Vol.24 No.3

This study uses the dynamic data envelopment analysis (DEA) model to evaluate intertemporalefficiency for high-tech industries in China. The significant difference from previous studies isthe assumption for patents, which are defined to be a carry-over intermediate linking differentterms. The model further provides an indicator of adjustment ratio for patents, based on theassessment of optimal quantity of patents. Output and input inefficiency indicators are alsodeveloped in the model to explore the sources of operational inefficiency. The empiricalresults conclude that intertemporal efficiency trends upward over time; the quantity ofpatents is assessed to be in shortage during the early terms, but excessive in recent terms;and that deficits in financial output is a significant factor in creating inefficiency.

Research on Home Stay Sharing Platform in Chinese Market Including Multinational Corporations : The Influence of Platform Operation on Customers’ Purchase Intention

Zou-Ji Kai,Guo-Han Wen 한국무역통상학회 2020 무역통상학회지 Vol.20 No.4

At present, the home-stay sharing industry all over the world is developing rapidly. Take the home-stay sharing industry in The Chinese market as an example, there are not only multinational companies like Airbnb, but also many local brands. This paper hopes to make an empirical study on the operation of the home-stay sharing industry platform in the Chinese market and draw a conclusion worthy of reference. Compared with traditional e-commerce, there are more obvious tripartite relationships among sellers, platforms and buyers in the Sharing business ecology. Among them, the intermediary and regulating role of Sharing business platform for the relationship between buyers and sellers becomes the core to ensure the sound operation of Sharing business. In this paper, users of the four leading platforms in the Chinese market, Airbnb, Tujia Home Stay and Xiaozhu Home Stay, are selected as research samples. The research model was validated by questionnaire survey and factor analysis. By studying the influence of the two core functions of Shared commercial platforms, “platform credit mechanism” and “platform personalized recommendation”, on the purchase intention of Sharing Home Stay tenant, effective Suggestions are proposed for the further improvement of the operation of Sharing accommodation platform.

Correction: A sandwich-like CMC-based/graphene/CMC-based conductive agent prepared from needle coke for high-performance LiFePO4 batteries

Zou Jin,Long Xi-Xi,He Jia-Le,Yu Shi-Peng,Zhong Sheng-Wen 한국탄소학회 2024 Carbon Letters Vol.34 No.3

Liquid phase exfoliation of natural graphite is an industrially effective solution for graphene preparation. However, many countries have identified natural graphite as a strategic resource and restricted its mining. In this report, we used abundant and readily available needle coke (NC) as a graphene exfoliation precursor and sodium carboxymethyl cellulose (CMC) as a dispersant to prepare a sandwich structured conductive graphitized NC nanosheets (GNCNs) by liquid phase exfoliation, freeze-drying and high-temperature graphitization, in which a graphene layer is sandwiched between two thin CMC layers. CMC could increase the liquid absorption and retention ability of the conductive agent and improve the migration rate of lithium ions. The highly ordered graphene layer could accelerate the transmission of electrons. The GNCNs with 0.4 wt% CMC addition showed good rate performance (144.6 mAh g−1 at 5 C) and high cycle stability (96.2% after 200 cycles at 1 C) for LiFePO4 (LFP) battery. The traditional Super-P (SP) conductive agent exhibited low-rate performance (113.9 mAh g−1 at 5 C) and cycle performance (89.9% after 200 cycles at 1 C). This study offers a novel approach to selecting graphene precursors and has

Preparation of BiPO4-Polyaniline Hybrid and its Enhanced Photocatalytic Performance

Wen Jie Yu,Yang Cheng,Tao Zou,Yi Liu,Kui Wu,Na Peng 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.1

Recently, BiPO4 has been proved to be an efficient photocatalyst which exhibits more attractive activity than that of TiO2-P25 under UV irradiation. However, photocatalytic oxidation-reduction ability of BiPO4 under visible light still needs to be improved. Conjugated polymer just like polyaniline was demonstrated to be a new and efficient visible co-photocatalyst in the field of pollutant governance. As far as we know, the attempt to enhance the photochemical ability of BiPO4 by employing polyaniline as a co-photocatalyst is still very limited. Hence, in order to testify the potential enhanced performance of such heterostructure, BiPO4-polyaniline heterostructure was prepared via hydrothermal method and hybridization in this paper. The experimental results indicated that several typical pollutants such as organic dyes and heavy metal ions can be effectively removed by BiPO4-polyaniline under visible light. The enhanced removal mechanism was attributed to the high separation efficiency of photo-induced electron–hole pairs and more efficient absorption of visible light after the loading of polyaniline on the surface of BiPO4 as an out-layer compared with single BiPO4.

Temperature control for a 5kW water-cooled PEMFC using fuzzy control

Wen-Jiang Zou,Young-Bae Kim 한국에너지학회 2018 한국에너지공학회 학술발표회 Vol.2018 No.11

Biomechanical Evaluation of 2 Endoscopic Spine Surgery Methods for Treating Lumbar Disc Herniation: A Finite Element Study

Yang Zou,Shuo Ji,Hui Wen Yang,Tao Ma,Yue Kun Fang,Zhi Cheng Wang,Miao Miao Liu,Ping Hui Zhou,Zheng Qi Bao,Chang Chun Zhang,Yu Chen Ye 대한척추신경외과학회 2024 Neurospine Vol.21 No.1

Objective: This study aimed to evaluate the effects of 2 endoscopic spine surgeries on the biomechanical properties of normal and osteoporotic spines. Methods: Based on computed tomography images of a healthy adult volunteer, 6 finite element models were created. After validating the normal intact model, a concentrated force of 400 N and a moment of 7.5 Nm were exerted on the upper surface of L3 to simulate 6 physiological activities of the spine. Five types of indices were used to assess the biomechanical properties of the 6 models, range of motion (ROM), maximum displacement value, intervertebral disc stress, maximum stress value, and articular protrusion stress, and by combining them with finite element stress cloud. Results: In normal and osteoporotic spines, there was no meaningful change in ROM or disc stress in the 2 surgical models for the 6 motion states. Model N1 (osteoporotic percutaneous transforaminal endoscopic discectomy model) showed a decrease in maximum displacement value of 20.28% in right lateral bending. Model M2 (unilateral biportal endoscopic model) increased maximum displacement values of 16.88% and 17.82% during left and right lateral bending, respectively. The maximum stress value of L4–5 increased by 11.72% for model M2 during left rotation. In addition, using the same surgical approach, ROM, maximum displacement values, disc stress, and maximum stress values were more significant in the osteoporotic model than in the normal model. Conclusion: In both normal and osteoporotic spines, both surgical approaches were less disruptive to the physiologic structure of the spine. Furthermore, using the same endoscopic spine surgery, normal spine biomechanical properties are superior to osteoporotic spines.