IL-17 Imbalance Promotes the Pyroptosis in Immune-Mediated Liver Injury Through STAT3-IFI16 Axis

Wenfang Xu,Yanan Wang,Changzhong Jin,Weiyang Zhang,Jiangnan Chen,Xuefang Chen,Junli Gao,Junshun Gao,Hong Wang 대한면역학회 2023 Immune Network Vol.23 No.6

Autoimmune hepatitis (AIH) affects all age group and occurs mainly in women. Pyroptosis is a novel programmed cell death featured with cell bursting and release of proinflammatory cytokines. A deeper understanding of AIH pathogenesis will contribute to novel therapy for AIH patients. Here, we aimed to investigate the role of IL-17 in immune-mediated liver injury. The levels of cytokines were measured by ELISA, and mRNA levels of STAT3 and IFN gammainducible protein 16 (IFI16) were detected by PCR. Expressions of STAT3, IFI16, gasdermin D and cleaved caspase-1 were measured by western-blotting. Immunohistochemical staining and transmission electron microscopy were applied to evaluate liver histopathological changes of the treated mice. Our results showed that the levels of IFI16 was increased in hepatocytes treated with IL-17 protein, and further elevated after STAT3-overexpressed (STAT3-OE) lentivirus treatment. The levels of IFI16 were reduced in hepatocytes treated with IL-17 neutralizing Ab (nAb), but were significantly increased after STAT3-OE treatment. Pyroptosis was observed in hepatocytes treated with IL-17 protein, and further cell damage was observed after STAT3-OE lentivirus treatment. Liver damage was alleviated in mice treated with IL-17 nAb, however sever damage was experienced after STAT3-OE lentivirus treatment. A binding interaction between IFI16 and STAT3 was detected in IL-17 treated hepatocytes. Glutathione transaminase activity was enhanced in concanavalin A-induced AIH mice compared to the control group (p<0.01). IL-17 plays an important role in activating STAT3 and up-regulating IFI16, which may promote the pyroptosis in AIH-related liver injury through STAT3-IFI16 axis.

Microstructure and Properties of BaTiO3

Min Wang,Guoge Zhang,Wenfang Li,Xiaojun Wang 대한화학회 2015 Bulletin of the Korean Chemical Society Vol.36 No.4

In this paper, we report the preparation of BaTiO3 (BT) ferroelectric films from barium hydroxide via micro arc oxidation (MAO) with additives. The BaTiO3 film was characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), micro-Raman spectroscopy, precision LCR measurements, and a Radiant Precision LC material analyzer. The results showed that the BT film was mainly composed of tetragonal-phase BaTiO3 , and the BT film was smooth, dense, and crack-free. At the frequency of 100 Hz, the film was found to possess a high dielectric constant of 245.3 and a low dielectric loss (tan δ) of 0.039. Compared with the BT film prepared without additives, the MAO-prepared film showed an increase of 61.1% in dielectric constant and a decrease of 59.4% in dielectric loss (tan δ). The BT film exhibited a saturated P–E hysteresis loop at room temperature.

Long-core experimental study of different displacement modes on fractured-vuggy carbonate reservoirs

Wenfang Li,Jiang Chen,Xiqun Tan,Ping Liu,Yun Li,Limin Wang 한국자원공학회 2018 Geosystem engineering Vol.21 No.2

It is difficult to utilize real fractured–vuggy carbonate cores to conduct long-core displacement experiments due to the special structures of fractured and vuggy. Aiming to not affect the porosity and permeability of the original core structure, a special filling method on the sunken space of the core surface was created to meet the requirements of long-core experiments. Long-core experiments of depletion drive, water flooding, gas drive, and water alternating gas (WAG) drive were performed to simulate the actual conditions of one-dimension oil reservoir and systematically study the influence of different development approaches on the recovery of vuggy carbonate reservoirs. Results show that the optimal conversion pressure of water flooding is between 80% bubble point and bubble point pressure. In the high-pressure and high water-cut stage, the development effect of gas–water alternative drive becomes significant, it can effectively reduce water cut and improve oil recovery. The most suitable and effective displacement mode is adopted depletion drive first and converts water flooding when the pressure decreases between 80% bubble point and bubble point pressure. When the water cut approximately reaches 80%, then WAG flooding is performed. The experiments provide data and technical support for the rational development of vuggy carbonate reservoirs.

Simultaneous Improvement of Thermal Conductivity and Strength for Commercial A356 Alloy Using Strontium Modification Process

Kang Wang,Wenfang Li,Wenzhi Xu,Shengyu Hou,Shaodong Hu 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.11

The Aluminum–Silicon (Al–Si) die-casting alloys, such as the commercial A356 alloy, are expected to be used in heat-sinkand the device with high thermal conductivity due to their high production efficiency of casting process. These fields alsorequired them to possess sound mechanical properties. To meet these demands, the Strontium (Sr) was often utilized tomodify the silicon phase. According to our current work, the secondary dendrite arm spacing of the α-Al grains prominentlywas decreased when 0.05%–0.25% strontium was added. The decrease of the secondary dendrite arm spacing (SDAS) lead tothe enhancement of the mechanical strength as well as the improvement of the thermal and electrical conductivity. When theSr dosage was 0.15% in the commercial A356 alloy, the strength and the thermal conductivity of the A356 alloy simultaneouslyreached the maximum value. The improvement of the electrical and thermal conductivity might by contributed by theformation of a good conductor, Al2Si2Srphase, on the Si surface. Further investigations suggested that the improvement ofthermal conductivity was mainly due to the modification effect of Sr on the eutectic Si phase, which enlarged the specificarea between the α-Al/eutectic Si interface. The WDS analysis indicated that the solubility of Si decreased in aluminummatrix by increasing in the Sr dosage. However, the excessive dosage of strontium would result in the coarsening of themodified silicon phase, deteriorating the strength and the thermal and electrical conductivity of the modified A356 alloys.

Removal of UO2 2+ from aqueous solution using halloysite nanotube-Fe3O4 composite

Yuantao Chen,Wenfang He,Wei Zhang,Chunlian Hu,Jian Wang,Pingping Wang 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.1

Halloysite nanotubes (HNTs) were modified with Fe3O4 to form novel magnetic HNTs-Fe3O4 composites, and the composites were characterized by X-ray diffraction (XRD), transmission electron microscope (TEM), Fourier transform infrared spectroscopy (FT-IR) and vibrating sample magnetometer (VSM). The as-obtained results indicated that Fe3O4 nanoparticles were successfully installed on the surface of HNTs. The adsorption of UO2 2+ on HNTs-Fe3O4 was investigated as a function of solid content, contact time, pH, ionic strength and temperature by batch experiments. The consequences revealed that the adsorption of UO2 2+ onto HNTs-Fe3O4 was strongly dependent on pH and ionic strength. Equilibrium data fitted well with the Langmuir isotherm. The experimental results demonstrated that the adsorbents with HNTs-Fe3O4 had the largest adsorption capacity of 88.32mg/g for UO2 2+.

Rapid adsorption of cobalt (II) by 3-aminopropyltriethoxysilane modified halloysite nanotubes

Yuantao Chen,Xin Wang,Wei Zhang,Wenfang He,Jian Wang,Biqing Chen 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.12

The adsorption of cobalt (II) by 3-aminopropyltriethoxysilane (APTS) modified halloysite nanotubes (HNTs) was studied. The modified halloysite was characterized by Fourier transform infrared spectroscopy (FT-IR), XRay diffraction (XRD) and thermogravimetry (TG). In addition, a batch method was used to study the adsorption behavior and the mechanism of removal Co (II) by modified HNTs; the effective factors influencing the adsorption process including adsorbent concentration, contact time, initial pH, temperature and the initial concentration of Co (II) have been investigated. The results showed that the APTS was successfully grafted on the HNTs surface. The modified HNTs exhibited rapid adsorption speed, which reached the adsorption equilibrium within 30 min. The adsorption capacity of the adsorbent increased significantly with the increase of pH. The adsorption data of Co (II) on the modified HNTs are well consistent with the Langmuir model and the pseudo-second-order kinetic model.

64kb/s Multi-function Terminal Manufactured for ISDN

Donghan Jin,Hongtao Wang,Wenfang Chen 대한전자공학회 1991 ICEIC:International Conference on Electronics, Inf Vol.1991 No.8

Experimental Study on the Bearing Characteristics of Rigid-Flexible Long-Short Pile Composite Foundations in Thick Collapsible Loess Areas

Fei Gao,Xuansheng Cheng,Wenfang Wang,Qingfeng Lv,Xiangjun Cheng 대한토목학회 2024 KSCE Journal of Civil Engineering Vol.28 No.5

The bearing and deformation characteristics of embankments with rigid-flexible long-short pile composite foundations (RLPCFs) in thick collapsible loess strata are not yet accurately understood. In this study, a large-scale field experiment was conducted, and screw (long) and compaction (short) piles were employed to reinforce a section of the foundation of the Lanzhou-Zhangye high-speed railway in thick collapsible loess. The pile load transfer, foundation settlement, pile-soil stress distribution, and load sharing characteristics were analyzed to reveal the bearing properties of the composite foundation. The results show that negative friction arises along the upper part of the pile, and the neutral points of the short pile and long pile are located at 2/5 and 1/3 down the pile lengths, respectively. The short pile eliminates the collapsibility of the shallow loess and enhances the foundation’s bearing capacity. The long pile transfers the load of the shallow foundation and pile top to the deep foundation through lateral friction, which reduces the settlement of the shallow foundation. When the soil arch in the embankment is fully formed, the short pile bears approximately 20% of the load, while the long pile and the soil between piles bear 80%. With the increase in embankment filling height, the load borne by the long pile rises, and the load borne by the soil between piles decreases gradually. The top settlement of the cross-section of the composite foundation is distributed in a concave basin shape, and the maximum settlement occurs in the center of the embankment. The parameters of the short pile can be obtained on the basis of the collapsibility grade and bearing capacity of the loess foundation, the length and area replacement rate of the long pile can be obtained based on the settlement control requirements of the superstructure of the composite foundation, and the lateral friction of the long pile can be increased by increasing the roughness of the pile and setting the screw.

Dynamics of Endogenous Polyamines in Seedling Trees of Pyrus ussuriensis Maxim. × P. bretschneideri Rehd

Chunmin Li,Xinzhong Zhang,Wenquan Yue,Yongbo Zhao,Wenfang Zu,Guangpeng Wang,Ruifeng Chang,Huairui Shu 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-