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      • KCI등재

        COMPLETELY GENERALIZED MILDLY NONLINEAR COMPLEMENTARITY PROBLEMS FOR FUZZY MAPPINGS

        Huang, Nan-Jing,Zhang, Wen-Bin 한국전산응용수학회 1997 Journal of applied mathematics & informatics Vol.4 No.2

        In this paper we introduce and study a new class of completely generalized mildly nonlinear complementarity problems for fuzzy mappings and construct some new iterative algorithms. We also show the existence of solution and the convergence of iterative sequences generated by the algorithm. Our results extend some recent results of Noor, Chang and huang.

      • KCI등재

        A case study of damage detection in four-bays steel structures using the HHT approach

        Wen-Ko Hsu,Cheng-Wu Chen,Dung-Jiang Chiou,Ming-Yi Liu,Wei-Ling Chiang,Pei-Chiung Huang 국제구조공학회 2014 Smart Structures and Systems, An International Jou Vol.14 No.4

        This study aims to investigate the relationship between structural damage and sensitivity indices using the Hilbert-Huang transform (HHT) method. Two damage detection indices are proposed: the ratio of bandwidth (RB), and the ratio of effective stiffness (RES). The nonlinear four bays multiple degree of freedom models with various predominant frequencies are constructed using the SAP2000 program. Adjusted PGA earthquake data (Japan 311, Chi-Chi 921) are used as the excitations. Next, the damage detection indices obtained using the HHT and the fast Fourier transform (FFT) methods are evaluated based on the acceleration responses of the structures to earthquakes. Simulation results indicate that, the column of the 1st floor is the first yielding position and the RB value is changed when the RES<90% in all cases. Moreover, the RB value of the 1st floor changes more sensitive than those from the top floor. In addition, when the structural response is nonlinear (i.e., RES<100%), the RB and the RES curves indicate the incremental change in the HHT spectra. However, the same phenomenon can be found from FFT spectra only when the stiffness reduction is large enough. Therefore, the RB estimated from the smoothed HHT spectra is an effective and sensitive index for detecting structural damage.

      • KCI등재

        Molecular cloning, expression profiles and subcellular localization of cyclin B in ovary of the mud crab, Scylla paramamosain

        Wen-Xing Li,Hui-Yang Huang,Jing-Ru Huang,Jin-Jin Yu,Jun Ma,Hai-Hui Ye 한국유전학회 2013 Genes & Genomics Vol.35 No.2

        A full-length cDNA of cyclin B was isolated from ovary of the mud crab (Scylla paramamosain) in this study. This transcript encodes a polypeptide of 401 amino acids, which is highly homologous to cyclin B protein family. Reverse transcription-PCR (RT-PCR) showed that cyclin B mRNA was expressed at highest levels in ovary of the mud crab. During the ovarian maturation process, realtime RT-PCR revealed that the abundance of cyclin B mRNA increased from the second stage (early-developing stage) to the fourth stage (nearly-ripe stage) and reached the peak level at the fifth stage (ripe stage). This result indicates the identified cyclin B gene might be related to the cell proliferation in ovary, both mitotically and meiotically. Immunohistochemistry showed that cyclin B protein was localized in the cytoplasm of prophase oocytes at the second stage while enriched in the nuclei of pro-metaphase oocytes at the fourth stage. It suggests the tested cyclin B protein might play different roles in ovary at the two stages.

      • Development of Sensing Signal Transmission and System in Intelligent Field

        ( Wen-luh Huang ),( Shu-yu Yang ),( Wen Sheng Chen ),( Yan-ru Huang ),( Yi-chen Wu ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1

        There is a problem which is large manpower consumption on contract sweet potato field when measure the soil moisture, control weeds, pests and diseases currently. In addition, serious problems of aging and labor shortage are especially troublesome in domestic rural areas. Therefore, we develop a management system combined sensor, single-chip control module and real-time irrigation module to meet the needs. Throughout the wireless signal transmission, the management of operating system with instant control and output data achieves intelligent control. At present, the system has been able to collect moisture data from field and to do the corresponding treatment immediately. At the same time, upload the data to cloud, then we can monitor field in real-time. With the establishment of the database, we have been to achieve long-term or short-term analysis and management. We really hope that the result can enhance the intelligence of agricultural technology, increase farmers profits, sustain development of agriculture and keep competitiveness.

      • ADPRT Val762Ala and XRCC1 Arg194Trp Polymorphisms and Risk of Gastric Cancer in Sichuan of China

        Wen, Yuan-Yuan,Pan, Xiong-Fei,Loh, Marie,Tian, Zhi,Yang, Shu-Juan,Lv, Si-Han,Huang, Wen-Zhi,Huang, He,Xie, Yao,Soong, Richie,Yang, Chun-Xia Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

        Objective: Gastric cancer remains a major health problem in China. We hypothesized that XRCC1 Arg194Trp and ADPRT Val762Ala may be associated with risk. Methods: We designed a multicenter 1:1 matched case-control study of 307 pairs of gastric cancers and controls between October 2010 and August 2011. XRCC1 Arg194Trp and ADPRT Val762Ala were sequenced, and demographic data as well as lifestyle factors were collected using a self-designed questionnaire. Results: Individuals carrying XRCC1 Trp/Trp or Arg/Trp variant genotype had a significantly increased risk of gastric cancer (OR, 1.718; 95% CI, 1.190-2.479), while the OR for ADPRT Val762Ala variant genotype (Ala/Ala or Val/Ala) was 1.175 (95% CI, 0.796-1.737). No gene-gene or gene-environment interactions were found. In addition, family history of cancer and drinkers proportion were higher among cases than among controls (P<0.05). Conclusions: XRCC1 194 Arg/Trp or Trp/Trp genotype, family history of cancer, and drinking are suspected risk factors of gastric cancer from our study. Our findings may offer insight into further similar large gene-environment and gene-gene studies in this region.

      • Streptomyces zhihengii sp. nov., isolated from rhizospheric soil of Psammosilene tunicoides

        Huang, Mei-Juan,Fei, Jing-Jing,Salam, Nimaichand,Kim, Chang-Jin,Hozzein, Wael N.,Xiao, Min,Huang, Hai-Quan,Li, Wen-Jun Springer-Verlag 2016 Archives of microbiology Vol.198 No.8

        <P>An actinomycete strain, designated YIM T102(T), was isolated from the rhizospheric soil of Psammosilene tunicoides W. C. Wu et C. Y. Wu collected from Lijiang, Yunnan Province, China. The taxonomic position of the new isolate was investigated by a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain YIM T102(T) belongs to the genus Streptomyces. Strain YIM T102(T) was most closely related to Streptomyces eurocidicus NRRL B-1676(T) with a pairwise 16S rRNA gene sequence similarity of 98.9 %. However, DNA-DNA relatedness value between strain YIM T102(T) and S. eurocidicus NBRC 13491(T) was found to be 37.8 +/- 1.8 %. The menaquinone composition detected for strain YIM T102(T) was MK-9 (H-6) and MK-9 (H-8), while the major fatty acids were summed feature 4 (38.0 %), anteiso-C-15:0 (13.1 %), iso-C-16:0 (10.1 %), summed feature 3 (9.8 %) and C-16:0 (9.0 %) and iso-C-15:0 (5.2 %). The whole-cell hydrolysates contained galactose, glucose, ribose and mannose, along with ll-diaminopimelic acid as the diagnostic diamino acid in the peptidoglycan. The DNA G+C content was 70.7 mol%. Strain YIM T102(T) also exhibited antagonistic activity against Alternaria alternata, Alternaria brassicae and Colletotrichum nicotianae Averna, based on the findings from the comparative analyses of phenotypic and genotypic characteristics; it is proposed that strain YIM T102 represents a novel species of the genus Streptomyces, for which the name Streptomyces zhihengii sp. nov. is proposed. The type strain is YIM T102(T) (=KCTC 39115(T) = DSM 42176(T) = CGMCC 4.7248(T)).</P>

      • TGF-β1 Protein Expression in Non-Small Cell Lung Cancers is Correlated with Prognosis

        Huang, Ai-Li,Liu, Shu-Guang,Qi, Wen-Juan,Zhao, Yun-Fei,Li, Yu-Mei,Lei, Bin,Sheng, Wen-Jie,Shen, Hong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19

        To investigate the expression intensity and prognostic significance of TGF-${\beta}1$ protein in non-small cell lung cancer (NSCLC), immunohistochemistry was carried out in 194 cases of NSCLC and 24 cases of normal lung tissues by SP methods. The PU (positive unit) value was used to assess the TGF-${\beta}1$ protein expression in systematically selected fields under the microscope with Leica Q500MC image analysis. We found that the TGF-${\beta}1$ PU value was nearly two-fold higher in NSCLC than in normal lung tissues (p=0.000), being associated with TNM stages (p=0.000) and lymph node metastases (p=0.000), but not to patient age, gender, smoking history, tumor differentiation, histological subtype and tumor location (P>0.05). Univariate analysis indicated that patients with high TGF-${\beta}1$ protein expression and lymph node metastases demonstrated a poor prognosis (both p=0.000,). Multivariate analysis showed that TGF-${\beta}1$ protein expression (RR = 2.565, p=0.002) and lymph node metastases (RR=1.874, p=0.030) were also independent prognostic factors. Thus, TGF-${\beta}1$ protein expression may be correlated to oncogenesis and serve as an independent prognostic biomarker for NSCLC.

      • KCI등재

        Ginsenoside Rg3 ameliorates allergic airway infl ammation and oxidative stress in mice

        Wen-Chung Huang,Tse-Hung Huang,Kuo-Wei Yeh,Ya-Ling Chen,Szu-Chuan Shen,Chian-Jiun Liou 고려인삼학회 2021 Journal of Ginseng Research Vol.45 No.6

        Background: Ginsenoside Rg3, isolated from Panax ginseng, has anti-inflammatory and anti-tumor activities. It is known to reduce inflammation in acute lung injury in mice, and to reduce the expression ofinflammatory cytokines and COX-2 in human asthmatic airway epithelium. In this study, we attemptedto determine whether ginsenoside Rg3 inhibits airway inflammation, oxidative stress, and airwayhyperresponsiveness (AHR) in the lungs of asthmatic mice. We also investigated its effects on oxidativestress and the inflammatory response in tracheal epithelial cells. Methods: Asthma symptoms were induced in female BALB/c mice sensitized with ovalbumin (OVA). Micewere divided into five groups: normal controls, OVA-induced asthmatic controls, and asthmatic micetreated with ginsenoside Rg3 or prednisolone by intraperitoneal injection. Inflammatory BEAS-2B cells(human tracheal epithelial cells) treated with ginsenoside Rg3 to investigate its effects on inflammatorycytokines and oxidative responses. Results: Ginsenoside Rg3 treatment significantly reduced eosinophil infiltration, oxidative responses,airway inflammation, and AHR in the lungs of asthmatic mice. Ginsenoside Rg3 reduced Th2 cytokineand chemokine levels in bronchoalveolar lavage fluids and lung. Inflammatory BEAS-2B cells treated withginsenoside Rg3 reduced the eotaxin and pro-inflammatory cytokine expressions, and monocyteadherence to BEAS-2B cells was significantly reduced as a result of decreased ICAM-1 expression. Furthermore, ginsenoside Rg3 reduced the expression of reactive oxygen species in inflammatory BEAS-2B cells. Conclusion: Ginsenoside Rg3 is a potential immunomodulator that can ameliorate pathological featuresof asthma by decreasing oxidative stress and inflammation

      • Hardening steels by the generation of transient phase using additive manufacturing

        Huang, E-Wen,Lee, Soo Yeol,Jain, Jayant,Tong, Yang,An, Ke,Tsou, Nien-Ti,Lam, Tu-Ngoc,Yu, Dunji,Chae, Hobyung,Chen, Shi-Wei,Chen, Shih-Min,Chou, Hung-Sheng Elsevier 2019 Intermetallics Vol.109 No.-

        <P><B>Abstract</B></P> <P>In the present work, the tensile properties of 15–5 PH steel fabricated by selective laser melting (SLM) were examined with respect to the transient austenite phase. Compared with the 8%-transient-phase sample, the 18%-transient-phase one shows higher ultimate tensile strength and relatively low yield strength, as well as hardening behavior. We conducted <I>in-situ</I> neutron-diffraction study to examine the microstructure evolution for mechanistic understanding. After applying the external load, most non-equilibrium, retained austenite in the 8%-transient-phase sample transforms before the yield strength, whereas in the 18%-transient-phase sample only 50% of the austenite transforms. Accompanying with the phase transformation, a decrease in the dislocation density and the dislocation strain energy-assisted phase transformation of the ferrite phase are found in the 8%-transient-phase sample even before yielding, which, however, is not the case in the 18%-transient-phase sample. The study demonstrates the SLM enables tuning the amount of transient-phase ratio and coherency between phases to realize a mechanical property control for additive manufactured steel.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The tensile behavior was examined for 8%- and 18%-transient-phase additive manufacturing steels using in-situ neutron diffraction. </LI> <LI> The 18%-transient-phase sample exhibited higher ultimate tensile strength and low yield strength. </LI> <LI> The 18%-transient-phase specimen with more non-equilibrium γ-phase revealed a more profound strain hardening effect. </LI> </UL> </P>

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