Mapping Signals That Are Important for Nuclear and Nucleolar Localization in MCRS2

Chung-Wen Kuo,Shih-Tung Liu,Wen-Hung Wang 한국분자세포생물학회 2011 Molecules and cells Vol.31 No.6

MCRS2 is an oncoprotein that is sequestered in the nu-cleolus. When in the nucleolus, it promotes the transcrip-tion of the rRNA gene. MCRS2 also brings proteins into the nucleolus to change their function. This study analyzes the sequence of MCRS2 and determines that the nuclear localization signal, which has the sequence KRKK, is situ-ated between amino acids 66 and 69. Meanwhile, MCRS2 contains a bipartite nucleolar localization signal, which comprises a KKSK motif, located between amino acids 133 and 136, and a downstream 152-amino acid region, from amino acid 314 to 465. The results of this study are impor-tant to understand the function of MCRS2.

Neuroprotective Activity of Vitis thunbergii var. taiwaniana Extracts In Vitro and In Vivo

Chung-Kwe Wang,Lih-Geeng Chen,Chi-Luan Wen,Wen-Chi Hou,Ling-Fang Hung,Shish-Jung Yen,Yi-Jyun Shen,Shyr-Yi Lin,Yu-Chih Liang 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.1

Vitis thunbergii var. taiwaniana (VTT) is a wild grape native to Taiwan, where it has been used as a folk medicine. In this study, we found that the branch and leaf ethanol extracts of VTT significantly inhibited the inducible nitric oxide (NO) synthase protein expression and NO production in BV2 microglia. Using primary neuronal cells, kainic acid (KA) significantly increased hydrogen peroxide production in a dose-dependent manner. All four ethanol extracts of VTT significantly decreased hydrogen peroxide production. However, only root and branch ethanol extracts were able to prevent the neuronal cell death induced by KA in vitro. In the animal study, administration of all four plant part extracts of VTT delayed the onset of seizure and decreased the hippocampus neuronal cell loss, and the neuroprotective activity could be ranked as follows: branchleaf>root>trunk. The results suggest that VTT extracts have a potential to prevent neurodegeneration through the antioxidative activity by their ability to inhibit NO and hydrogen peroxide production.

Hints from a Female Patient with Breast Cancer Who Later Presented with Cowden Syndrome

Wen-Chung Wang,Tai-Cheng Hou,Chen-Yun Kuo,Yen-Chein Lai 한국유방암학회 2020 Journal of breast cancer Vol.23 No.4

A 51-year-old woman presented with metachronous tumor development in bilateral breasts, thyroid, and endometrium. Additional signs and symptoms fulfilled the National Comprehensive Cancer Network criteria for Cowden syndrome. Immunohistochemistry showed loss of PTEN expression in all tumors. Single nucleotide variants, 647 germline variants (including one each in PTEN and MSH3), and 21 somatic mutations within exons were detected in all tumors after whole-exome sequencing. There were 0, 11, and 46 specific somatic mutations in bilateral breasts, thyroid, and endometrial cancers, respectively. Although PTEN mutation is key to the development of Cowden syndrome, DNA repair dysfunction might be the initial driver of mutations. Fewer mutations were required to induce initial bilateral breast carcinomas, with subsequent thyroid and endometrial carcinomas requiring more mutations for induction. When genetic screening is unavailable, breast cancer patients with clinical manifestations of Cowden syndrome must be carefully assessed for secondary malignancies, such as thyroid and endometrial carcinomas.

眞理的堅持

汪中文(Chung- wen Wang),沈美綺(Mei-chi Shen) 대구대학교 인문과학연구소 2012 人文科學硏究 Vol.38 No.-

Yin-ke Chen, from Yi-ning, Jiang-shi in China, was not only a history specialist but also a knowledgeable master in Chinese Classics. His statements on the memorial of Guan-tang Wang from Ching-hua University says, ”The study principle for a learned person is to glorify truth by freeing the mind from the control of the secularity. I’d rather die without the freedom of thought.” It is clear to see from his life that he was a typical figure, with the thoughts of freedom, who faced spiritual and physical frustration when there was a political reformation in China. However, the political constraint could never change his strong mind toward the insistence on free will and independent spirit. A huge number of his poems express his persistent spirit on culture. To explore this spirit, his poems are provided in this study

Detection of edge delamination in surface adhered active fiber composites

Wang, Dwo-Wen,Yin, Ching-Chung Techno-Press 2009 Smart Structures and Systems, An International Jou Vol.5 No.6

A simple method has been developed to detect the bonding condition of active fiber composites (AFC) adhered to the surface of a host structure. Large deformation actuating capability is one of important features of AFC. Edge delamination in adhesive layer due to large interfacial shear stress at the free edge is typically resulted from axial strain mismatch between bonded materials. AFC patch possesses very good flexibility and toughness. When an AFC patch is partially delaminated from host structure, there remains sensing capability in the debonded part. The debonding size can be determined through axial resonance measured by the interdigitated electrodes symmetrically aligned on opposite surfaces of the patch. The electrical impedance and modal response of the AFC patch in part adhered to an aluminum plate were investigated in a broad frequency range. Debonding ratio of the AFC patch is in inverse proportion to the resonant frequency of the fundamental mode. Feasibility of in-situ detecting the progressive delamination between AFC patch and host plate is demonstrated.

Red Bean Extract Inhibits Lipopolysaccharide-Induced Inflammation and H2O2-Induced Oxidative Stress in RAW 264.7 Macrophages

Wen-Wan Chao,Yun-Chin Chung,I-Ping Shih,Hsun-Yen Wang,Su-Tze Chou,Cheng-Kuang Hsu 한국식품영양과학회 2015 Journal of medicinal food Vol.18 No.7

Red bean (Phaseolus radiatus L. var. Aurea) is a leguminous seed and mainly used as one of the popular ingredients in oriental desserts. The objective of this study was to evaluate the anti-inflammatory activity of 50 g/kg ethanolic extract of red bean (RBE) by measuring lipopolysaccharide (LPS)-induced expressions of nitric oxide (NO), inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) in RAW 264.7 macrophages. On the other hand, the antioxidant activity of RBE was determined by thiobarbituric acid reactive substances method and comet assay using H2O2-induced macrophages. The results showed that RBE at the concentrations of 50–200 μg/mL can significantly suppress the inflammatory responses in LPS-stimulated macrophages through the reduction of cellular NO and downregulation of the gene expressions of iNOS, COX-2, TNF-α, and IL-6 in a dose-dependent manner. Furthermore, RBE can diminish H2O2-induced oxidative damage in RAW 264.7 macrophage. Phenolic compounds and cyanidin-3-Oglucoside from BRE may have efficacy as overall in vitro anti-inflammatory and antioxidant agents. Red bean exerts an antiinflammatory response and has potential as a health-promoting ingredient.

The Effects of Outward Foreign Direct Investment on Fixed-Capital Formation at Home: The Roles of Host Location and Industry Characteristics

Wen Chung Hsu,Chengqi Wang,Jeremy Clegg 연세대학교 동서문제연구원 2015 Global economic review Vol.44 No.3

This paper pursues the idea that the relationship between foreign and domestic investments may be not as uniform as many studies suggest. By examining the case of Taiwanese outward foreign direct investment (OFDI), this paper is marked out from existing studies in the following three respects. First, it examines the extent to which the relationship between OFDI and domestic investment varies with the location of investment. Second, this research allows the results to vary between Heckscher–Ohlin (H–O) industries and Schumpeter industries. Finally, its breakdown of data will reveal sub-relationships in the data that up to now have remained hidden within the aggregate relationships reported in most studies. This study suggests that OFDI in China has a positive impact on domestic investment in H–O industries, while OFDI in other countries (OFDIO) has a negative impact on domestic investment in the same industries. These findings are in marked contrast to Schumpeter industries where a positive effect is observed only for OFDIO. Our findings also suggest that the Taiwanese government should design policies to adjust the level of liberalisation for overseas investment through legislation on an industry-by-industry basis in order that OFDI stimulates domestic investment in relevant industries more effectively.

Proton Pump Inhibitor-unresponsive Laryngeal Symptoms Are Associated With Psychological Comorbidities and Sleep Disturbance: A Manometry and Impedance-pH Monitoring Study

Wen-Hsuan Tseng,Wei-Chung Hsu,Tsung-Lin Yang,Tzu-Yu Hsiao,Jia-Feng Wu,Hui-Chuan Lee,Hsiu-Po Wang,Ming-Shiang Wu,Ming-Shiang Wu,Ping-Huei Tseng 대한소화기 기능성질환∙운동학회 2023 Journal of Neurogastroenterology and Motility (JNM Vol.29 No.3

Background/AimsLaryngeal symptoms are largely treated with empiric proton pump inhibitor (PPI) therapy if no apparent pathology shown on ear, nose, and throat evaluation and reflux-related etiologies are suspected. However, treatment response remains unsatisfactory. This study aimed to investigate the clinical and physiological characteristics of patients with PPI-refractory laryngeal symptoms. MethodsPatients with persistent laryngeal symptoms despite PPI treatment for ≥ 8 weeks were recruited. A multidisciplinary evaluation comprising validated questionnaires for laryngeal symptoms (reflux symptom index [RSI]), gastroesophageal reflux disease symptoms, psychological comorbidity (5-item brief symptom rating scale [BSRS-5]) and sleep disturbance (Pittsburgh sleep quality index [PSQI]), esophagogastroduodenoscopy, ambulatory impedance-pH monitoring, and high-resolution impedance manometry were performed. Healthy asymptomatic individuals were also recruited for comparison of psychological morbidity and sleep disturbances. ResultsNinety-seven adult patients and 48 healthy volunteers were analyzed. The patients had markedly higher prevalence of psychological distress (52.6% vs 2.1%, P < 0.001) and sleep disturbance (82.5% vs 37.5%, P < 0.001) than the healthy volunteers. There were significant correlations between RSI and BSRS-5 scores, and between RSI and PSQI scores (r = 0.26, P = 0.010, and r = 0.29, P = 0.004, respectively). Fifty-eight patients had concurrent gastroesophageal reflux disease symptoms. They had more prominent sleep disturbances (89.7% vs 71.8%, P < 0.001) than those with laryngeal symptoms alone but similar reflux profiles and esophageal motility. ConclusionsPPI-refractory laryngeal symptoms are mostly associated with psychological comorbidities and sleep disturbances. Recognition of these psychosocial comorbidities may help optimize management in these patients.

THE RELATION BETWEEN SANDSTORMS AND STRONG WINDS IN XINJIANG, CHINA

WANG, XU,MA, YU,CHEN, HONGWU,WEN, GANG,CHEN, SHOUJUN,TAO, ZUYU,CHUNG, YONG-SEUNG 한국교원대학교 환경과학연구소 2004 환경연구논문집 Vol.- No.8

With observational data spanning 1961-1999 from 90 meteorological stations in Xinjiang, China, the spatial and temporal characteristics of sandstorms and strong winds, and the contribution of strong winds to the occurrence of sandstorms are analyzed. Moreover, the dominant wind direction and minimum wind speeds during sandstorm periods are discussed. The research shows chat although possessing similar climatic trends, sandstorms and strong winds in Xinjiang have opposite geographical distributions, i.e. places with more sandstorms show fewer strong winds. The contribution of strong winds to sandstorms in northern Xinjiang is larger than that in southern Xinjiang. The dominant wind directions clerarly indicate the paths of the weather systems that introduced the sandstorms. The minimum wind speeds in the sandstoims were ova 10 ms^(-1) in northern and eastern Xinjiang and in the Turpan and Yanqi Basins of southern Xinjiang. In Tarim Basin of southern Xinjiang, however, the minimum wind speed was about 6-8 ms^(-1), and even 6 ms^(-1) at its southero edge.

Detection of edge delamination in surface adhered active fiber composites

Dwo-Wen Wang,Ching-Chung Yin 국제구조공학회 2009 Smart Structures and Systems, An International Jou Vol.5 No.6

A simple method has been developed to detect the bonding condition of active fiber composites(AFC) adhered to the surface of a host structure. Large deformation actuating capability is one of important features of AFC. Edge delamination in adhesive layer due to large interfacial shear stress at the free edge is typically resulted from axial strain mismatch between bonded materials. AFC patch possesses very good flexibility and toughness. When an AFC patch is partially delaminated from host structure, there remains sensing capability in the debonded part. The debonding size can be determined through axial resonance measured by the interdigitated electrodes symmetrically aligned on opposite surfaces of the patch. The electrical impedance and modal response of the AFC patch in part adhered to an aluminum plate were investigated in a broad frequency range. Debonding ratio of the AFC patch is in inverse proportion to the resonant frequency of the fundamental mode. Feasibility of in-situ detecting the progressive delamination between AFC patch and host plate is demonstrated.