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Weiyi Shao,Wenxian Zhu,Yanhua Wang,Jingwei Lu,Ge Jin,Yixin Wang,Wenli Su 한국생물공학회 2016 Biotechnology and Bioprocess Engineering Vol.21 No.1
Human pancreatic trypsin (hPT) is an established target for acute pancreatitis (AP) therapeutics. Here, a bioinformatics protocol of protein docking, peptide refinement, dynamics simulation and affinity analysis was described to perform rational design and molecular engineering of hPT peptide aptamers. Protein docking was employed to model the intermolecular interactions between hPT and its cognate inhibitory protein, the human pancreatic trypsin inhibitor (hTI). A number of peptide fragments were cut out from the interaction sites of docked hPT–hTI complexes, from which a decapeptide fragment 13LNGCTLEYRP22 was found to exhibit potent inhibition against hPT (Ki = 5.3 ± 0.8 μM). We also carried out alanine scanning and virtual mutagenesis to systematically examine the independent contribution of peptide residues to binding affinity, and the harvested knowledge were then used to guide modification and optimization of the decapeptide fragment. Subsequently, inhibition studies of nine promising candidates against recombinant hPT were conducted, from which four samples were successfully identified to have high or moderate potency (Ki < 10 μM). In particular, the peptides LQVCTLEYCN and LQICTLEYCT were found to inhibit hPT activity significantly (Ki = 0.23 ± 0.04 and 0.85 ± 0.18 μM, respectively). Structural analysis of hPT– peptide complex systems unraveled diverse chemical interactions such as hydrogen bonds, salt bridges and hydrophobic forces across the complex interfaces.
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Liu Weiyi,Wang Fang,Sun Yanmei,Yang Lei,Chen Huihai,Liu Weijie,Zhu Bin,Hui Chaomao,Wang Shiwei 한국미생물학회 2020 The journal of microbiology Vol.58 No.11
Dragon bamboo (Dendrocalamus giganteus) is a giant sympodial bamboo species widely distributed in Asia. However, it remains unclear how dragon bamboo and soil microbes interact to affect soil properties. In this study, we investigated the planting patterns (semi-natural and artificial) on different slopes (sunny and shady) to determine the effects on soil properties and microbial community. The results showed that the soil in which dragon bamboo was grown was acidic, with a pH value of ~5. Also, the soil organic matter content, nitrogen hydrolysate concentration, total nitrogen, available potassium, and total potassium of the dragon bamboo seminatural forest significantly improved, especially on the sunny slope. In contrast, the available phosphorus level was higher in the artificial bamboo forest, probably owing to the phosphate fertilizer application. The bacterial and fungal diversity and the bacterial abundance were all higher on the sunny slope of the semi-natural forest than those in the other samples. The microbial operational taxonomic units (OTUs) shared between the shady and sunny slopes accounted for 47.8–62.2%, but the core OTUs of all samples were only 24.4– 30.4% of each sample, suggesting that the slope type had a significant effect on the microbial community. Some acidophilic microbes, such as Acidobacteria groups, Streptomyces and Mortierella, became dominant in dragon bamboo forest soil. A PICRUSt analysis of the bacterial functional groups revealed that post-translational modification, cell division, and coenzyme transport and metabolism were abundant in the semi-natural forest. However, some microorganisms with strong stress resistance might be activated in the artificial forest. Taken together, these results illustrated the influence of dragon bamboo growth on soil physicochemical property and microbial community, which might help understand the growth status of dragon bamboo under different planting patterns.
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Research on Optimization Adjustment Strategy for SaaS Multi-tenant Data Placement
Li Xiaona,Li Qingzhong,Zhu Weiyi,Li Hui 보안공학연구지원센터 2015 International Journal of Grid and Distributed Comp Vol.8 No.2
In order to meet the requirements for data access by tenants and management by service providers, the multi-tenant data stored in cloud using replica technology must be reasonably placed. For the outweight nodes and the ultra light nodes, according to characteristics of the multi-tenant data and the load of nodes, through adjusting the number and position of the replicas to maintenance and optimization the strategy so that meet the SLA requirements meanwhile minimize the overall cost. Experimental results through comparison with random placement strategy and greedy placement policy demonstrate the feasibility and effectiveness of the proposed strategy.
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Prevalence of Depression in Ankylosing Spondylitis: A Systematic Review and Meta-Analysis
Lijuan Zhang,Yaping Wu,Shiguang Liu,Weiyi Zhu 대한신경정신의학회 2019 PSYCHIATRY INVESTIGATION Vol.16 No.8
The aim of this study was to provide a summary estimate of depression prevalence among people with ankylosing spondylitis (AS) in comparison to those without AS. A systematic literature search was conducted using PubMed, Embase, PsycINFO, Web of Science, the Cochrane database library, China National Knowledge Infrastructure, and Wanfang Database from their inception to December 2016. The results showed that thirty-one eligible studies involving 8,106 patients were analyzed. Fifteen methods of defining depression were reported. The overall pooled prevalence of depression was 35% (95% CI, 28–43%), with high between-study heterogeneity (I2=98.8%, p<0.001). The relative risk of depression among people with AS was 1.76 (95% CI: 1.21–2.55, eight studies, n=3,006) compared with people without AS. The depression score [standardized mean difference (SMD)=0.43, 95% CI: 0.19–0.67, seven studies, n=549] was higher in AS patients than in controls. The main influence on depression prevalence was the sample size and country of origin. In conclusion, one-third of people with AS experience symptoms of depression. Depression was more prevalent in AS patients than in controls. Further research is needed to identify effective strategies for preventing and treating depression among AS patients.
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