Extracellular signal-regulated kinase 5 increases radioresistance of lung cancer cells by enhancing the DNA damage response

Weiwei Jiang,Guanghui Jin,Fangfang Cai,Xiao Chen,Nini Cao,Xiangyu Zhang,Jia Liu,Fei Chen,Feng Wang,Wei Dong,Hongqin Zhuang,Zichun Hua 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

Radiotherapy is a frequent mode of cancer treatment, although the development of radioresistance limits its effectiveness. Extensive investigations indicate the diversity of the mechanisms underlying radioresistance. Here, we aimed to explore the effects of extracellular signal-regulated kinase 5 (ERK5) on lung cancer radioresistance and the associated mechanisms. Our data showed that ERK5 is activated during solid lung cancer development, and ectopic expression of ERK5 promoted cell proliferation and G2/M cell cycle transition. In addition, we found that ERK5 is a potential regulator of radiosensitivity in lung cancer cells. Mechanistic investigations revealed that ERK5 could trigger IR-induced activation of Chk1, which has been implicated in DNA repair and cell cycle arrest in response to DNA double-strand breaks (DSBs). Subsequently, ERK5 knockdown or pharmacological inhibition selectively inhibited colony formation of lung cancer cells and enhanced IR-induced G2/M arrest and apoptosis. In vivo, ERK5 knockdown strongly radiosensitized A549 and LLC tumor xenografts to inhibition, with a higher apoptotic response and reduced tumor neovascularization. Taken together, our data indicate that ERK5 is a novel potential target for the treatment of lung cancer, and its expression might be used as a biomarker to predict radiosensitivity in NSCLC patients.

Let-7c miRNA Inhibits the Proliferation and Migration of Heat-Denatured Dermal Fibroblasts Through Down-Regulating HSP70

Jiang, Tao,Wang, Xingang,Wu, Weiwei,Zhang, Fan,Wu, Shifeng Korean Society for Molecular and Cellular Biology 2016 Molecules and cells Vol.39 No.4

Wound healing is a complex physiological process necessitating the coordinated action of various cell types, signals and microRNAs (miRNAs). However, little is known regarding the role of miRNAs in mediating this process. In the present study, we show that let-7c miRNA is decreased in heat-denatured fibroblasts and that inhibiting let-7c expression leads to the increased proliferation and migration of dermal fibroblasts, whereas the overexpression of let-7c exerts an opposite effect. Further investigation has identified heat shock protein 70 as a direct target of let-7c and has demonstrated that the expression of HSP70 in fibroblasts is negatively correlated with let-7c levels. Moreover, down-regulation of let-7c expression is accompanied by up-regulation of Bcl-2 expression and down-regulation of Bax expression, both of which are the downstream genes of HSP70. Notably, the knockdown of HSP70 by HSP70 siRNA apparently abrogates the stimulatory effect of let-7c inhibitor on heat-denatured fibroblasts proliferation and migration. Overall, we have identified let-7c as a key regulator that inhibits fibroblasts proliferation and migration during wound healing.

Infinite time interval RBSDEs with non-Lipschitz coefficients

Weiwei Hua,Long Jiang,Xuejun Shi 한국통계학회 2013 Journal of the Korean Statistical Society Vol.42 No.2

This paper aims at solving infinite horizon reflected backward stochastic differential equations (RBSDEs) under weak assumptions. We obtain a more general existence and uniqueness theorem about the solutions to RBSDEs with non-Lipschitz generators.

Reusable, magnetic laser-induced graphene for efficient removal of organic pollutants from water

Jiang Ye,Wan Sijie,Zhao Weiwei,Yu Wenjie,Wang Shuaipeng,Yu Zeqi,Yang Qiu,Zhou Weihua,Liu Xiaoqing 한국탄소학회 2022 Carbon Letters Vol.32 No.4

The hybridization of graphene with magnetic nanoparticles has endowed graphene with increasing interest as the adsorbent for wastewater treatment. However, its fabrication often involves a multi-stepped chemical synthesis process. In this work, we demonstrate a facile, one-step, and solvent-free approach to fabricate Fe3O4 nanoparticle-anchored Laser-Induced Graphene (Fe3O4@LIG) as an efficient adsorbent by direct laser irradiation on a ferric acetylacetonate containing polybenzoxazine film. Raman and X-ray diffraction analysis confirm the graphene component in the adsorbent, and the morphology characterizations show that Fe3O4 nanoparticles are distributed uniformly on LIG with hierarchical meso- and macro-porous structures. Adsorption experiments indicate that Fe3O4@LIG can adsorb methylene blue (MB) from aqueous solutions in a fast and effective manner, with a maximum adsorption capacity up to 350.9 mg/g. The adsorption kinetics and isotherms are also investigated, which are well-described by the pseudo-second-order model and Langmuir model, respectively. Additionally, Fe3O4@LIG is also demonstrated with the efficient removal of a variety of organic solvents from water. The favorable adsorption behavior of Fe3O4@LIG is attributed to its unique porous structure and the molecular interactions with adsorbates. On the other hand, Fe3O4@LIG has high magnetic property, and therefore, it could be easily recovered from water and well regenerated for repeated use. With the efficient adsorption of organic pollutants, magnetic separability, and good recyclability, it is believed that the easy-fabricated Fe3O4@LIG has great potential applications in wastewater treatment.

Origin of Improved Photoelectrochemical Water Splitting in Mixed Perovskite Oxides

Li, Weiwei,Jiang, Kai,Li, Zhongguo,Gong, Shijing,Hoye, Robert L. Z.,Hu, Zhigao,Song, Yinglin,Tian, Chuanmu,Kim, Jongkyoung,Zhang, Kelvin H. L.,Cho, Seungho,MacManus-Driscoll, Judith L. Wiley (John WileySons) 2018 ADVANCED ENERGY MATERIALS Vol.8 No.31

Modeling Knot Properties for Mongolian Pine in Northeast China

Jia, Weiwei,Li, Fengri,Jiang, Lichun Korean Society of Forest Science 2008 한국산림과학회지 Vol.97 No.5

This study was performed in 14 unthinned Mongolian pine (Pinus sylvestris L. var. mongolica Litvin) plantations in northeast China. Data were collected on 70 sample trees of different canopy position with diameter at breast height (DBH) ranging from 6.9 cm to 34.5 cm. Diameter and length of knots per whorl below the living crown were studied by different vertical levels divided by relative knot height (RHK) in this paper. Models taking DBH and height to the crown base (HCB) as independent variables were developed to predict knot diameter (KD) in a sample whorl. According to the vertical distribution tendency and range of sound knot length (KLsound), KLsound was modeled as multiple linear function of DBH, KD and relative knot height (RHK). The loose knot length (KLloose) was described as a function of DBH, KD and height above the ground for knots (HK) in a mixed log-linear model. Results from this study can provide abundant knot information so as to describe the knot size and vertical distribution tendency of Mongolian pine plantation.

Analysis of Status of Smartphone Photography and Socialization among the Elderly People in Nanjing Community

Jiabei Jiang,Weiwei Zhang,Jihong Jeung,Yuhao Huang 한국HCI학회 2020 한국HCI학회 학술대회 Vol.2020 No.2

With the ubiquity of smart devices and the aging population,more and more elderly people begin to use smart devices. Their main application ranges from phone calls and messaging to payment and navigation. Many studies have been conducted on the evaluation of usability of smart device for the elderly. However, few of them were focused on the characteristics and difficulties of the elderly people in smartphone photography. This study took the elderly people as the target to research on their experience of using photo-shooting applications. Based on questionnaires and interviews, combined with qualitative and quantitative researches, this paper explores the social influence of taking pictures with smartphones on both the physical and the psychological aspects. The results show: 1)The majority of elderly people use smartphone photography; gender and living conditions are the main factors affecting shooting experience, while age is not; Smartphone photography has changed the way they think and their life habits; 2)Very few of the elderly share their photos on social network service(SNS) because of the risk of privacy leakage and mis-operation; while viewing family and friends’ photos could bring them senses of participation; 3)The main difficulties for the elderly are album management and the lack of affordance of the interface elements; 4)The needs of the elderly people in smartphone photography cannot be ignored.

Visible-Light Self-Driven Photodetector with Enhanced Performance Based on CdS /Si Nanowire Heterojunction

Yurong Jiang,Haifa Zhai,Weiwei Cao,Haigang Yang,Hairui Liu 대한금속·재료학회 2016 ELECTRONIC MATERIALS LETTERS Vol.12 No.6

In this article, the appealing application of the CdS/Si nanowire heterostructure asthe self-driven photodetector was presented based on a facile successive ioniclayer absorption and reaction. Compared to CdS /Si planar based device,photocurrent illuminated with light (532 nm) to dark-current ratio was enhancedsignificantly for CdS/Si nanowire based detector. Measurements of time resolvedresponses at zero bias were conducted, and the result also demonstrated that CdS/Si nanowire heterostructure had a high photosensitivity and fast photo-responseas the self-driven visible light photodetector. Additionally, the correspondingmechanism for the phenomenon was discussed, and it is believed that such a highphotoresponse comes from the improved optical absorption as well as the goodcarrier transfer and collection capability. The present CdS/Si core-shellheterojunction architectures are envisaged as potentially valuable candidates fornext-generation photodectors.

Fe-curcumin Nanozyme-Mediated Immunosuppression and Anti-inflammation in Experimental Autoimmune Uveitis

Zhengxuan Jiang,Kun Liang,Xiang Gao,Fan Cao,Guangqi An,Siyu Gui,Weiwei Tang,Liping Du,Liming Tao,Xianwen Wang 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Background EAU is an inflammatory disease usually characterized by autoinflammation and autoimmunity and is aggravated by excessive generation of ROS. Conventional hormone therapy often has more adverse effects. It is urgent to find a therapeutic drug with higher efficiency and fewer adverse effects. Methods We developed an Fe-curcumin nanozyme in which natural antioxidants coordinate with Fe3+ to form nanoparticles with excellent solubility for directing anti-inflammatory and ROS scavenging effects to treat EAU. Several experiments were used to detect the characteristics of nanozymes. EAU model rats were used to detect the abilities of decreasing autoinflammation and autoimmunity. PBMCs were used to detect the ability to inhibit cell proliferation. Results Free radical scavenging experiments showed that nanozymes decreased the level of free radicals at low concentrations. In vitro and in vivo experiments revealed that the group treated with Fe-curcumin nanozymes had lower inflammatory reactions and ROS levels than the control group, as reflected by the downregulated levels of several critical inflammatory cytokines, such as IFN-γ, IL-17, and TNF-α; decreased H2O2 release; inhibited proliferation of Th1 and Th17 cells; and alleviated pathological changes in the eye. Importantly, the Fe-curcumin nanozyme was detected in the retina using Prussian blue staining. Additionally, Fe-curcumin nanozyme is noncytotoxic when directing these biological activities. Conclusion This study has demonstrated the feasibility of using the Fe-curcumin nanozyme as a nanodrug to inhibit inflammatory reactions and scavenge ROS in the treatment of EAU, indicating that it may serve as a promising therapeutic agent in clinical treatment.

Hydrothermal Synthesis of Hierarchical Hollow Li(Ni0.8Co0.15Al0.05)O2 Microspheres for Lithium Ion Batteries

Liu Wenlong,Jiang Weiwei,Yan Chenran,Feng Wei,Wang Dongxia,Zhao Liming,Feng Tingting,Gong Feng 대한화학회 2017 Bulletin of the Korean Chemical Society Vol.38 No.11

Li(Ni0.8Co0.15Al0.05)O2 (NCA) cathode material for lithium ion batteries is synthesized by calcining Ni–Co–Al hydroxide or carbonate hydroxide hydrate precursor, which is obtained by hydrothermal method, and the NCA cathode material is hierarchical hollow microspheres with the diameter in the range of 3–5 µm and composed of nanoflakes and/or nanowires. The effect of hydrothermal reaction time on the morphologies and the crystalline quality of the NCA cathode material is investigated on the basis of scanning electron microscopy, X-ray diffraction, and tunneling electron microscopy. The NCA cathode material shows smooth and monotonous charge/discharge characteristics, delivers a reversible capacity as 144.4 mAh/g and retains 90.4% after 50 cycles at a current density of 0.1 C between 2.5 and 4.3 V.