Gene expression and promoter methylation of porcine uncoupling protein 3 gene

Ruiyi Lin,Weimin Lin,Qiaohui Chen,Jianchao Huo,Yuping Hu,Junxiao Ye,Jingya Xu,Tianfang Xiao 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.2

Objective: Uncoupling protein 3 gene (UCP3) is a candidate gene associated with the meat quality of pigs. The aim of this study was to explore the regulation mechanism of UCP3 expression and provide a theoretical basis for the research of the function of porcine UCP3 gene in meat quality. Methods: Bisulfite sequencing polymerase chain reaction (PCR) and quantitative real-time PCR (Q-PCR) were used to analyze the methylation of UCP3 5′-flanking region and UCP3 mRNA expression in the adipose tissue or skeletal muscle of three pig breeds at different ages (1, 90, 210-day-old Putian Black pig; 90-day-old Duroc; and 90-day-old Dupu). Results: Results showed that two cytosine-guanine dinucleotide (CpG) islands are present in the promoter region of porcine UCP3 gene. The second CpG island located in the core promoter region contained 9 CpG sites. The methylation level of CpG island 2 was lower in the adipose tissue and skeletal muscle of 90-day-old Putian Black pigs compared with 1-day-old and 210-day-old Putian Black pigs, and the difference also existed in the skeletal muscle among the three 90-day-old pig breeds. Furthermore, the obvious changing difference of UCP3 mRNA expression was observed in the skeletal muscle of different groups. However, the difference of methylation status and expression level of UCP3 gene was not significant in the adipose tissue. Conclusion: Our data indicate that UCP3 mRNA expression level was associated with the methylation status of UCP3 promoter in the skeletal muscle of pigs.

試論中國左翼文學的審美特征

林?民(Lin Weimin) 중국문화연구학회 2003 중국문화연구 Vol.0 No.2

On Trans-boundary Damages

Lin, Canling,Tian, weimin 한국환경법학회 2004 環境法 硏究 Vol.26 No.1

A Comparison Study of Kinematic and Dynamic Models for Trajectory Tracking of Autonomous Vehicles Using Model Predictive Control

Bao-Lin Ye,Shaofeng Niu,Lingxi Li,Weimin Wu 제어·로봇·시스템학회 2023 International Journal of Control, Automation, and Vol.21 No.9

Efficient trajectory tracking approaches can enable autonomous vehicles not only to get a smooth trajectory but to achieve a lower energy dissipation. Since vehicle model plays an important role in trajectory tracking, this paper investigates and compares the performance of two classical vehicle models for trajectory tracking of autonomous vehicles using model predictive control (MPC). Firstly, a two-degree-of-freedom kinematic model and a three-degree-of-freedom yaw dynamic model are established for autonomous vehicles. Meanwhile, in order to carry out tracking control more effectively and smoothly, the tire slip angle has been taken into account by the dynamic model. Then, we design two MPC controllers for trajectory tracking, which are based on the kinematic model and the dynamic model, respectively. The performances of two MPC controllers are evaluated and compared on the Carsim/Matlab joint simulation platform. Experimental results demonstrated that, under low-speed working conditions, both two MPC controllers can follow the reference trajectory with high accuracy and stability. However, under high-speed working conditions, the tracking error of the kinematic model is too large to be used in the real trajectory tracking problem. On the contrary, the controller based on the dynamic model still performs a good tracking effect. In addition, this study offers guidance on how to select a suitable vehicle model for autonomous vehicles under different speed working conditions.

Active Disturbance Rejection Control Based on Extended State Observer for Three-Phase Battery Energy Storage Power Conversion System with LCL Filter

Gao Ning,Lin Xin,Lu Song,Xia Yue,Wu Weimin 대한전기학회 2022 Journal of Electrical Engineering & Technology Vol.17 No.2

Utilizing battery energy storage system is considered to be a reliable approach to improve the stability of modern power grid. By properly controlling the battery energy storage system, the redundant power can be absorbed and the lack of power can be supplemented. The control strategy of power conversion system directly aff ects the performance of battery energy storage system. Therefore, an improved active disturbance rejection control algorithm for power conversion system with LCL fi lter is presented in this paper. First of all, a pole placement strategy based on full state feedback is implemented in order to solve the resonance problem of LCL fi lter. Secondly, the total internal and external disturbances of power conversion system are compensated on basis of a linear extended state observer for purpose of improving the system robustness against inevitable interferences and parameter uncertainty. Furthermore, the stability of presented control strategy is analyzed and discussed in detail. Finally, the simulation and experimental results extracted based on a 2.6 kVA prototype are both provided, which can confi rm the feasibility and correctness of the control strategy.

Differentiating Pancreatic Ductal Adenocarcinoma from Pancreatic Serous Cystadenoma, Mucinous Cystadenoma, and a Pseudocyst with Detailed Analysis of Cystic Features on CT Scans: a Preliminary Study

Peijie Lv,Radfan Mahyoub,Xiaozhu Lin,Kemin Chen,Weimin Chai,Jing Xie 대한영상의학회 2011 Korean Journal of Radiology Vol.12 No.2

Objective: To determine whether or not detailed cystic feature analysis on CT scans can assist in the differential diagnosis of pancreatic ductal adenocarcinoma (PDAC) from serous cystadenoma (SCN), mucinous cystadenoma (MCN), and a pseudocyst. Materials and Methods: This study received Institutional Review Board approval and informed patient consent was waived. Electronic radiology and pathology databases were searched to identify patients with PDAC (n = 19), SCN (n = 26), MCN (n = 20) and a pseudocyst (n = 23) who underwent pancreatic CT imaging. The number, size, location, and contents of cysts,and the contour of the lesions were reviewed, in addition to the wall thickness, enhancement patterns, and other signs of pancreatic and peripancreatic involvement. Diagnosis was based on lesion resection (n = 82) or on a combination of cytological fi ndings, biochemical markers, and tumor markers (n = 6). Fisher’s exact test was used to analyze the results. Results: A combination of the CT findings including irregular contour, multiple cysts, mural nodes, and localized thickening, had a relatively high sensitivity (74%) and specifi city (75%) for differentiating PDAC from SCN, MCN, and pseudocysts (p < 0.05). Other CT fi ndings such as location, greatest dimension, or the presence of calcifi cation were not signifi cantly different. Conclusion: The CT fi ndings for PDAC are non-specifi c, but perhaps helpful for differentiation. PDAC should be included in the general differential diagnosis of pancreatic cystic neoplasms.

Computerized bone age estimation system based on China-05 standard

Yin, Chuangao,Zhang, Miao,Wang, Chang,Lin, Huihui,Li, Gengwu,Zhu, Lichun,Fei, Weimin,Wang, Xiaoyu Techno-Press 2022 Advances in nano research Vol.12 No.2

The purpose of this study is to develop an automatic software system for bone age evaluation and to evaluate its accuracy in testing and feasibility in clinical practice. 20394 left-hand radiographs of healthy children (2-18 years old) were collected from China Skeletal Development Survey data of 1998 and China Skeletal Development Survey data of 2005. Three experienced radiologists and China-05 standard maker jointly evaluate the stages of bone development and the reference bone age was determined by consensus. 1020 from 20394 radiographs were picked randomly as test set and the remaining 19374 radiographs as training set and validation set. Accuracy of the automatic software system for bone age assessment is evaluated in test set and two clinical test sets. Compared with the reference standard, the automatic software system based on RUS-CHN for bone age assessment has a 0.04 years old mean difference, ±0.40 years old in 95% confidence interval by single reading, a 85.6% percentage agreement of ratings, a 93.7% bone age accuracy rate, 0.17 years old of MAD, 0.29 years old of RMS; Compared with the reference standard, the automatic software system based on TW3-C RUS has a 0.04 years old mean difference, a ±0.38 years old in 95% confidence interval by single reading, a 90.9% percentage agreement of ratings, a 93.2% bone age accuracy rate, a 0.16 years of MAD, and a 0.28 years of RMS. Automatic software system, AI-China-05 showed reliably accuracy in bone age estimation and steady determination in different clinical test sets.

A stable and highly efficient visible-light-driven hydrogen evolution porous CdS/WO₃/TiO₂ photocatalysts

Qian, Yongteng,Yang, Mengke,Zhang, Fangfang,Du, Jimin,Li, Kaidi,Lin, Xialing,Zhu, Xinrui,Lu, Yayun,Wang, Weimin,Kang, Dae Joon Elsevier 2018 Materials characterization Vol.142 No.-

<P><B>Abstract</B></P> <P>It is well known that both catalytic efficiency and stability are the two important parameters of photocatalysts for visible-light-driven hydrogen production reactions. However, light-driven hydrogen evolution based applications still suffer from sluggish reaction kinetics due to the lack of high-performance photocatalysts. In this paper, we successfully synthesized a ternary porous CdS/WO<SUB>3</SUB>/TiO<SUB>2</SUB> photocatalyst with high efficiency and stability via two-stage approach. The as-prepared samples are characterized by XRD, FESEM, EDS, TEM, XPS, and UV–Vis, respectively, which illustrated that the CdS and WO<SUB>3</SUB> moieties are in-situ formed inside the porous TiO<SUB>2</SUB>. Particularly, the photocatalytic hydrogen (H<SUB>2</SUB>) evolution rate of such ternary 8% CdS/WO<SUB>3</SUB>/TiO<SUB>2</SUB> (molar ration of CdS:WO<SUB>3</SUB>:TiO<SUB>2</SUB> = 8:8:100) photocatalyst ranges up to 2106 μmol h<SUP>−1</SUP> g<SUP>−1</SUP> under visible-light irradiation, which is higher than that of pure TiO<SUB>2</SUB> and other binary (CdS/TiO<SUB>2</SUB> and WO<SUB>3</SUB>/TiO<SUB>2</SUB>) porous photocatalysts. The superior H<SUB>2</SUB> evolution efficiency can be attributed to the coexistence of CdS and WO<SUB>3</SUB> in porous TiO<SUB>2</SUB> which can promote the interfacial charge transfer and separation as well as extend the light absorption up to the visible range.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Highly efficient and stable photocatalytic activity of CdS/WO<SUB>3</SUB>/TiO<SUB>2</SUB> photocatalysts were synthesized by a two-step method. </LI> <LI> CdS and WO<SUB>3</SUB> inlaid on porous TiO<SUB>2</SUB> can extend the light absorption and enhance photogenerated electron-hole pairs separation. </LI> <LI> The porous structure can provide more reaction active sites and improve photoproduced-electrons and holes transport speed. </LI> </UL> </P> <P><B>Graphical Abstract</B></P> <P>[DISPLAY OMISSION]</P>