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Spirosoma jeollabukense sp. nov., isolated from soil
Li, Weilan,Ten, Leonid N.,Lee, Seung-Yeol,Lee, Dong Hoon,Jung, Hee-Young Springer-Verlag 2018 Archives of microbiology Vol.200 No.3
<P>A Gram-negative, non-motile, rod-shaped, aerobic bacterial strain, designated S2-3-6(T), was isolated from soil in Jeollabuk-do province, South Korea, and was characterized taxonomically using a polyphasic approach. According to a comparative 16S rRNA gene sequence analysis, strain S2-3-6(T) belonged to the family Cytophagaceae and was most closely related to Spirosoma endophyticum EX36(T) (98.2%), Spirosoma fluviale MSd3(T) (96.0%), and Spirosoma linguale DSM 74(T) (95.7%). Strain S2-3-6(T) contained summed feature 3 (C16:1 omega 7c/C16:1 omega 6c), C16:1 omega 5c, and C16:0 N alcohol as major cellular fatty acids, MK-7 as the predominant respiratory quinone, and phosphatidylethanolamine as the main polar lipid. The DNA G+C content of strain S2-3-6(T) was 47.8%. Phenotypic and chemotaxonomic data supported the affiliation of strain S2-3-6(T) to the genus Spirosoma. However, the DNA-DNA relatedness between strain S2-3-6(T) and Spirosoma endophyticum KACC 17920(T) was 27%, clearly showing that the isolate constitutes a new genospecies. Strain S2-3-6(T) could be clearly differentiated from its closest neighbors based on its phenotypic, genotypic, and chemotaxonomic features. Therefore, strain S2-3-6(T) represents a novel species of the genus Spirosoma, for which the name Spirosoma jeollabukense sp. nov. is proposed. The type strain is S2-3-6(T) (= KCTC 52725(T) = JCM 32129(T)).</P>
Weilan Li,이승열,Chang-Gi Back,Leonid N. Ten,Hee-Young Jung 한국식물병리학회 2019 Plant Pathology Journal Vol.35 No.6
To detect Xanthomonas arboricola pv. pruni, a loopmediated isothermal amplification (LAMP) detection method were developed. The LAMP assay was designed to test crude plant tissue without pre-extraction, or heating incubation, and without advanced analysis equipment. The LAMP primers were designed by targeting an ABC transporter ATP-binding protein, this primer set was tested using the genomic DNA of Xanthomonas and non-Xanthomonas strains, and a ladder product was generated from the genomic DNA of X. arboricola pv. pruni strain but not from 12 other Xanthomonas species strains and 6 strains of other genera. The LAMP conditions were checked with the healthy leaves of 31 peach varieties, and no reaction was detected using either the peach leaves or the peach DNA as a template. Furthermore, the high diagnostic accuracy of the LAMP method was confirmed with 13 X. arboricola pv. pruni strains isolated from various regions in Korea, with all samples exhibiting a positive reaction in LAMP assays. In particular, the LAMP method successfully detected the pathogen in diseased peach leaves and fruit in the field, and the LAMP conditions were proven to be a reliable diagnostic method for the specific detection and identification of X. arboricola pv. pruni in peach orchards.
Spirosoma pomorum sp. nov., isolated from apple orchard soil
Weilan Li,이승열,강인규,Leonid N. Ten,정희영 한국미생물학회 2018 The journal of microbiology Vol.56 No.2
A Gram-negative, motile, rod-shaped, aerobic bacterial strain, designated S7-2-11T, was isolated from apple orchard soil from Gyeongsangnam-do Province, Republic of Korea, and was characterized taxonomically using a polyphasic approach. 16S rRNA gene sequence analysis indicated that strain S7-2- 11T belongs to the family Cytophagaceae in phylum Bacteroidetes, and is closely related to Spirosoma luteolum 16F6ET (94.2% identity), Spirosoma knui 15J8-12T (92.7%), and Spirosoma linguale DSM 74T (91.0%). The G + C content of the genomic DNA of strain S7-2-11T was 49.8 mol%. Strain S7-2-11T contained summed feature 3 (C16:1 ω7c/C16:1 ω6c; 35.1%), C16:1 ω5c (22.4%), C15:0 iso (13.9%), and C17:0 iso 3-OH (10.6%) as major cellular fatty acids, and MK-7 as the predominant respiratory quinone. The main polar lipids were phosphatidylethanolamine, an unidentified aminophospholipid, and two unidentified polar lipids. Phenotypic and chemotaxonomic data supported the affiliation of strain S7-2-11T with the genus Spirosoma. The results of physiological and biochemical tests showed the genotypic and phenotypic differentiation of the isolate from recognized Spirosoma species. On the basis of its phenotypic properties, genotypic distinctiveness, and chemotaxonomic features, strain S7-2-11T represents a novel species of the genus Spirosoma, for which the name Spirosoma pomorum sp. nov. is proposed. The type strain is S7-2-11T (= KCTC 52726T = JCM 32130T).
First Report of Leptosphaerulina australis Isolated from Soil in Korea
( Weilan Li ),( Chang-gi Back ),( Seung-yeol Lee ),( Leonid N. Ten ),( Hee-young Jung ) 한국균학회 2018 韓國菌學會誌 Vol.46 No.4
The fungal strain KNU16-004 was isolated from a field soil sample collected in Seoul. The isolate was identified as Leptosphaerulina australis based on morphological characterization and phylogenetic analysis using the internal transcribed spacer (ITS), large subunit (LSU) rDNA regions, and β-tubulin (Tub2). This is the first report of Leptosphaerulina australis in Korea.
Li, Weilan,Lee, Seung-Yeol,Back, Chang-Gi,Ten, Leonid N.,Jung, Hee-Young The Korean Society of Plant Pathology 2019 Plant Pathology Journal Vol.35 No.6
To detect Xanthomonas arboricola pv. pruni, a loopmediated isothermal amplification (LAMP) detection method were developed. The LAMP assay was designed to test crude plant tissue without pre-extraction, or heating incubation, and without advanced analysis equipment. The LAMP primers were designed by targeting an ABC transporter ATP-binding protein, this primer set was tested using the genomic DNA of Xanthomonas and non-Xanthomonas strains, and a ladder product was generated from the genomic DNA of X. arboricola pv. pruni strain but not from 12 other Xanthomonas species strains and 6 strains of other genera. The LAMP conditions were checked with the healthy leaves of 31 peach varieties, and no reaction was detected using either the peach leaves or the peach DNA as a template. Furthermore, the high diagnostic accuracy of the LAMP method was confirmed with 13 X. arboricola pv. pruni strains isolated from various regions in Korea, with all samples exhibiting a positive reaction in LAMP assays. In particular, the LAMP method successfully detected the pathogen in diseased peach leaves and fruit in the field, and the LAMP conditions were proven to be a reliable diagnostic method for the specific detection and identification of X. arboricola pv. pruni in peach orchards.
Occurrence of Bacterial Stem Rot of Ranunculus asiaticus Caused by Pseudomonas marginalis in Korea
Li, Weilan,Ten, Leonid N.,Kim, Seung-Han,Lee, Seung-Yeol,Jung, Hee-Young The Korean Society of Plant Pathology 2018 식물병연구 Vol.24 No.2
In December 2016, stem rot symptoms were observed on Persian buttercup (Ranunculus asiaticus) plants in Chilgok, Gyeongbuk, Korea. In the early stage of the disease, several black spots appeared on the stem of infected plants. As the disease progressed, the infected stem cleaved and wilted. The causal agent was isolated from a lesion and incubated on Reasoner's 2A (R2A) agar at $25^{\circ}C$. Total genomic DNA was extracted for phylogenetic analysis. Based on the 16S rRNA gene analysis, the isolated strain was found to belong to the genus Pseudomonas. To identify the isolated bacterial strain at the species level, the nucleotide sequences of the gyrase B (gyrB) and RNA polymerase D (rpoD) genes were obtained and compared with the sequences in the GenBank database. As the result, the causal agent of the stem rot disease was identified as Pseudomonas marginalis. To determine the pathogenicity of the isolated bacterial strain, it was inoculated into the stem of healthy R. asiaticus plant, the inoculated plant showed a lesion with the same characteristics as the naturally infected plant. Based on these results, this is the first report of bacterial stem rot on R. asiaticus caused by P. marginalis in Korea.