Metabolomes and transcriptomes revealed the saponin distribution in root tissues of Panax quinquefolius and Panax notoginseng

Wei, Guangfei,Yang, Feng,Wei, Fugang,Zhang, Lianjuan,Gao, Ying,Qian, Jun,Chen, Zhongjian,Jia, Zhengwei,Wang, Yong,Su, He,Dong, Linlin,Xu, Jiang,Chen, Shilin The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.6

Background: Panax quinquefolius and Panax notoginseng are widely used and well known for their pharmacological effects. As main pharmacological components, saponins have different distribution patterns in the root tissues of Panax plants. Methods: In this study, the representative ginsenosides were detected and quantified by desorption electrospray ionization mass spectrometry and high-performance liquid chromatography analysis to demonstrate saponin distribution in the root tissues of P. quinquefolius and P. notoginseng, and saponin metabolite profiles were analyzed by metabolomes to obtain the biomarkers of different root tissues. Finally, the transcriptome analysis was performed to demonstrate the molecular mechanisms of saponin distribution by gene profiles. Results: There was saponin distribution in the root tissues differed between P. quinquefolius and P. notoginseng. Eight-eight and 24 potential biomarkers were detected by metabolome analysis, and a total of 340 and 122 transcripts involved in saponin synthesis that were positively correlated with the saponin contents (R > 0.6, P < 0.05) in the root tissues of P. quinquefolius and P. notoginseng, respectively. Among them, GDPS1, CYP51, CYP64, and UGT11 were significantly correlated with the contents of Rg1, Re, Rc, Rb2, and Rd in P. quinquefolius. UGT255 was markedly related to the content of R1; CYP74, CYP89, CYP100, CYP103, CYP109, and UGT190 were markedly correlated with the Rd content in P. notoginseng.

Analysis of Different Activation Statuses of Human Mammary Epithelial Cells from Young and Old Groups

Feng, Chen-Chen,Chen, Li-Na,Chen, Mei-Jun,Li, Wan,Jia, Xu,Zhou, Yan-Yan,He, Wei-Ming Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.8

Human mammary epithelial cells have different proliferative statuses and demonstrate a close relationship with age and cell proliferation. Research on this topic could help understand the occurrence, progression and prognosis of breast cancer. In this article, using significance analysis of a microarray algorithm, we analyzed gene expression profiles of human mammary epithelial cells of different proliferative statuses and different age groups. The results showed there were significant differences in gene expression in the same proliferation status between elderly and young groups. Three common differentially expressed genes were found to dynamically change with the proliferation status and to be closely related to tumorigenesis. We also found elderly group had less status-related differential genes from actively proliferating status to intermediate status and more statusrelated differential genes from intermediate status than the young group. Finally, functional enrichment analyses allowed evaluation of the detailed roles of these differentially-expressed genes in tumor progression.

Studies on the Distribution Characteristic and Assessment of the Soil Nutrition in the Yellow River Delta New-born Wetlands

( Wei Feng Chen ),( Yan Xi Shi ),( Yong Ji Piao ) 한국녹지환경디자인학회 2005 녹지환경학회지 Vol.1 No.1

This paper investigates preliminarily spatial distribution soil organic matter(SOM), nitrogen(N) and phosphorus(P) and its environmental influence in wetland soil of different vegetation landscape in the Yellow River Mouth. The result shows the SOM and TN, efficient N, efficient P in top layer soils of different vegetation district have significantly different content, The SOM is shown as Calamagrostis epigeios wetlands>Phragmites communis wetlands>Tamarix chinensis wetlands above tidal>Suaeda salsa wetlands in high tidal>Tamarix chinensis wetlands in high tidal>tidal flats, the arrange of the TN and efficient N content is the same except that the content in Suaeda salsa wetlands in high tidal is heavier than Tamarix chinensis wetlands in high tidal. But the difference obvious regularity of soil nutrient of ground floor. In different vegetation landscape wetland types the vertical change of soil nutrients are obvious except for P, gradually decrease from the upper to the lower. This case reflects the function of the vegetation on the wetland development of soil, and proves the wetland soil has the Characteristic of new-born and bad degree of development. SOM, total nitrogen (TN), efficient N and efficient P content in wetland soils have significantly positive correlation, but TP have no correlation with them but efficient P. The contents of TN in wetland soils range from 58~1,480mg/kg, total average content 408mg/kg, average content of above 30cm is 625mg/kg, The range of TP content in the soil is 372~1,042mg/kg, total average is 569mg/kg, average content of above 20cm is 611 mg/kg. According the P It occurs mainly ascalcium phosphates, and the validity is lower, therefore, N and P in the new-born wetlands cannot produce serious impact on the environments at present.

2022 Taiwan clinical multicenter expert consensus and recommendations for thyroid radiofrequency ablation

Wei-Che Lin,Wen-Chieh Chen,Pei-Wen Wang,Yi-Chia Chan,Yen-Hsiang Chang,Harn-Shen Chen,Szu-Tah Chen,Wei-Chih Chen,Kai-Lun Cheng,Shun-Yu Chi,Pi-Ling Chiang,Chen-Kai Chou,Feng-Fu Chou,Shun-Chen Huang,Feng 대한초음파의학회 2023 ULTRASONOGRAPHY Vol.42 No.3

Radiofrequency ablation (RFA) is a minimally invasive management strategy that has been widely applied for benign and recurrent malignant thyroid lesions as an alternative to surgery in Taiwan. Members of academic societies for specialists in interventional radiology, endocrinology, and endocrine surgery collaborated to develop the first consensus regarding thyroid RFA in Taiwan. The modified Delphi method was used to reach a consensus. Based on a comprehensive review of recent and valuable literature and expert opinions, the recommendations included indications, pre-procedural evaluations, procedural techniques, post-procedural monitoring, efficacy, and safety, providing a comprehensive review of the application of RFA. The consensus effectively consolidates advice regarding thyroid RFA in clinical practice for local experts.

Analysis of Small Fragment Deletions of the APC gene in Chinese Patients with Familial Adenomatous Polyposis, a Precancerous Condition

Chen, Qing-Wei,Zhang, Xiao-Mei,Zhou, Jian-Nong,Zhou, Xin,Ma, Guo-Jian,Zhu, Ming,Zhang, Yuan-Ying,Yu, Jun,Feng, Ji-Feng,Chen, Sen-Qing Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.12

Background: : Familial adenomatous polyposis (FAP) is an autosomal dominant inherited disease mainly caused by mutations of the adenomatous polyposis coli (APC) gene with almost complete penetrance. These colorectal polyps are precancerous lesions that will inevitable develop into colorectal cancer at the median age of 40-year old if total proctocolectomy is not performed. So identification of APC germline mutations has great implications for genetic counseling and management of FAP patients. In this study, we screened APC germline mutations in Chinese FAP patients, in order to find novel mutations and the APC gene germline mutation characteristics of Chinese FAP patients. Materials and Methods: The FAP patients were diagnosed by clinical manifestations, family histories, endoscope and biopsy. Then patients peripheral blood samples were collected, afterwards, genomic DNA was extracted. The mutation analysis of the APC gene was conducted by direct polymerase chain reaction (PCR) sequencing for micromutations and multiplex ligation-dependent probe amplification (MLPA) for large duplications and/or deletions. Results: We found 6 micromutations out of 14 FAP pedigrees, while there were no large duplications and/or deletions found. These germline mutations are c.5432C>T(p. Ser1811Leu), two c.3926_3930delAAAAG (p.Glu1309AspfsX4), c.3921_3924delAAAA (p.Ile1307MetfsX13), c3184_3187delCAAA(p.Gln1061AspfsX59) and c4127_4126delAT (p.Tyr1376LysfsX9), respectively, and all deletion mutations resulted in a premature stop codon. At the same time, we found c.3921_3924delAAAA and two c.3926_3930delAAAAG are located in AAAAG short tandem repeats, c3184_3187delCAAA is located in the CAAA interrupted direct repeats, and c4127_4128 del AT is located in the 5'-CCTGAACA-3', 3'-ACAAGTCC-5 palindromes (inverted repeats) of the APC gene. Furthermore, deletion mutations are mostly located at condon 1309. Conclusions: Though there were no novel mutations found as the pathogenic gene of FAP in this study, we found nucleotide sequence containing short tandem repeats and palindromes (inverted repeats), especially the 5 bp base deletion at codon 1309, are mutations in high incidence area in APC gene,.

Slow Release Fertilizer Decreases Leaching Loss of Nitrogen in Sand-based Root Zone

Chen Wei-Feng(첸웨이펭),Wang-Wei(왕웨이),Qi Ying-Jie(퀴잉제) 한국잡초학회·한국잔디학회 2007 Weed & Turfgrass Science Vol.21 No.2

축구장의 식재지반이 모래로 조성될 경우 낮은 보비력과 높은 용탈율로 인해 시비관리의 조정이 필요하다. 본 실험의 목적은 PVC관을 이용한 경기장 유사모형 실험구에서 캔터키 블루그래스에 있어서 완효성비료의 생육 효과를 비교 실험하였다. 잔디의 색깔, 균일도, 생육, 지상부 생체량과배출수의 질산의 유출량을 몇가지 다른 생육상과 네번의 유사 강우 시험 시 행하였다. 실험 결과 요소의 성분 유출량이 가장 높았고 공시 완효성비료는 가장 낮았다. 이 완효성 비료는 다른 공사 잔디비료에 비해 14일간, 요소보다는 28일간 잔디의 품질 변에서 비효가 지속되었다. 또한 이 완효성비료는 잔디의 웃자람과 잔디깎기에 소요되는 시간을 줄일 수 있었다. 질산태 질소의 유출에 았어서도잔디의 조성 초반기에 50% 정도를 감소 시켰다. When a football field is constructed using sand medium, the fertilizer management has to be adjusted because of the low nutrient holding capacity and higher leaching rate. The objective of this study was to test the effects of slow release fertilizers on Kentucky bluegrass (Poa pratensis L.) growth in simulated sport field rootzones with PVC pipe pots. Data of turf grass color, uniformity, growth rate, biomass above ground, and the nitrate content in the leaching solution was collected at different growing stages and during four simulated rain fall periods. The result showed that the nutrient release rate of urea was the highest and that of controlled release nitrogen fertilizer was the lowest. Effects of the controlled release nitrogen fertilizer lasted 14 days more than other lawn fertilizers and 28 days longer than regular urea with acceptable quality levels of turf. The slow release fertilizer also restrained excessive growth of the grass, reduced the times of mowing. Slow release fertilizer used in this study reduced NO₃-N leaching by almost 50% at the beginning of turf establishment.

Inhibitory Effects of α-Pinene on Hepatoma Carcinoma Cell Proliferation

Chen, Wei-Qiang,Xu, Bin,Mao, Jian-Wen,Wei, Feng-Xiang,Li, Ming,Liu, Tao,Jin, Xiao-Bao,Zhang, Li-Rong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.7

Background: Pine needle oil from crude extract of pine needles has anti-tumor effects, but the effective component is not known. Methods: In the present study, compounds from a steam distillation extract of pine needles were isolated and characterized. Alpha-pinene was identified as an active anti-proliferative compound on hepatoma carcinoma BEL-7402 cells using the MTT assay. Results: Further experiments showed that ${\alpha}$-pinene inhibited BEL-7402 cells by arresting cell growth in the G2/M phase of the cell cycle, downregulating Cdc25C mRNA and protein expression, and reducing cycle dependence on kinase 1(CDK1) activity. Conclusion: Taken together, these findings indicate that ${\alpha}$-pinene may be useful as a potential anti-tumor drug.

Updated Meta-analysis on HER2 Polymorphisms and Risk of Breast Cancer: Evidence from 32 Studies

Chen, Wei,Yang, Heng,Tang, Wen-Ru,Feng, Shi-Jun,Wei, Yun-Lin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.22

Background: Several studies have been performed to investigate the association of the HER2 Ile655Val polymorphism and breast cancer risk. However, the results were inconsistent. To understand the precise relationship, a meta-analysis was here conducted. Materials and Methods: A search of PubMed conducted to investigate links between the HER2 Ile655Val polymorphism and breast cancer, identified a total of 32 studies, of which 29, including 14,926 cases and 15,768 controls, with odds ratios (ORs) with 95% confidence intervals were used to assess any association. Results: In the overall analysis, the HER2 Ile655Val polymorphism was associated with breast cancer in an additive genetic model (OR=1.136, 95% CI 1.043-1.239, p=0.004) and in a dominant genetic (OR=1.118, 95% CI 1.020-1.227, p=0.018), while no association was found in a recessive genetic model. On subgroup analysis, an association with breast cancer was noted in the additive genetic model (OR=1.111, 95% CI: 1.004-1.230, p=0.042) for the Caucasian subgroup. No significant associations were observed in Asians and Africans in any of the genetic models. Conclusions: In summary, our meta-analysis findings suggest that the HER2 Ile655Val polymorphism is marginally associated with breast cancer susceptibility in worldwide populations with additive and dominant models, but not a recessive model.

DSLA: Dynamic Sampling Localization Algorithm Based on Virtual Anchor Node

( Yanru Chen ),( Bingshu Yan ),( Liangxiong Wei ),( Min Guo ),( Feng Yin ),( Qian Luo ),( Wei Wang ),( Liangyin Chen ) 한국인터넷정보학회 2019 KSII Transactions on Internet and Information Syst Vol.13 No.10

Compared with the localization methods in the static sensor networks, node localization in dynamic sensor networks is more complicated due to the mobility of the nodes. Dynamic Sampling Localization Algorithm Based on Virtual Anchor (DSLA) is proposed in this paper to localize the unknown nodes in dynamic sensor networks. Firstly, DSLA algorithm predicts the speed and movement direction of nodes to determine a sector sampling area. Secondly, a method of calculating the sampling quantity with the size of the sampling area dynamically changing is proposed in this paper. Lastly, the virtual anchor node, i.e., the unknown node that got the preliminary possible area (PLA), assists the other unknown nodes to reduce their PLAs. The last PLA is regarded as a filtering condition to filter out the conflicting sample points quickly. In this way, the filtered sample is close to its real coordinates. The simulation results show that the DSLA algorithm can greatly improve the positioning performance when ensuring the execution time is shorter and the localization coverage rate is higher. The localization error of the DSLA algorithm can be dropped to about 20%.

Selective and Full Derivatization of Amino Group in Chitosan with Alkyl Chloroformate of Low Stereo-Hindrance

Zi-Wei Feng,Shao-Hua Huang,Wei Chen,Zheng-Wu Bai 한국고분자학회 2016 Macromolecular Research Vol.24 No.7