Real-Time Repairable Interpolation Scheme for CNC Tool Path Processing

Wei Fan,Chen-Han Lee,Ji-Hong Chen 한국정밀공학회 2016 International Journal of Precision Engineering and Vol.17 No.12

G01 trajectory is widely-adopted for CNC machining. The piecewise linear trajectory is geometrically discontinuous, bringing about drastic fluctuation of feedrate and acceleration, and causing low machining efficiency and quality. Many methods of trajectory smoothing are developed and applied in CNC system. However some shortcomings exist when these methods were applied in CNC machining. In the existing methods, the following requirements are not satisfied simultaneously: not less than G2 continuity, confined chord error, shape preservation, interpolating G01 points, spatial applicability, real-time performance of algorithm, free geometric form. Considering various requirements of CNC machining, this work develops an interpolation scheme with local repairing function to deal with the possible shape deficiencies of trajectory. The resulting tool path has G2 continuity and is optimized in bending energy under shape-preserving and chord error constraints. Combining with jerk-limited feedrate scheduling, a real-time tool path processing strategy is developed. The simulation demonstrates the proposed method eliminates shape deficiencies, and has apparent advantage in the reduction of contour error, curvature, bending energy and machining time, compared with Zhao’s and Fan’s methods. The experiment demonstrates the advantages of the proposed algorithm in improving the quality of machined surface and machining efficiency, compared with previous methods.

Experimental Studies on Progressive Collapse Behavior of RC Frame Structures: Advances and Future Needs

Wei?Jian Yi,Fan Yi,Yun Zhou 한국콘크리트학회 2021 International Journal of Concrete Structures and M Vol.15 No.4

In the recent two decades, the progressive collapse of reinforced concrete (RC) frame structures attracted unprecedented research interests in the structural engineering community. Experiments are regarded as an essential method in this field since actual cases can barely provide sufficient and effective data to support rigorous research. In this paper, prevailing experimental assumptions and configurations among over 100 series of experiments are quantitatively revealed by a bibliometric collection based on systematic search in an academic database. Since numerous experiments have been reported on the progressive collapse of RC frame structures, this paper subsequently presents a state-of-the-art review summarizing both experimental consensuses and controversies constituted by three main aspects: (a) static mechanisms, (b) dynamic behavior, and (c) threat-dependent research. The significance of secondary mechanisms, existing problems of dynamic effects, and potential flaws of the threat-independent assumption are discussed in detail with experimental findings. Future needs are emphasized on research targets, correlations between experiments and design, dynamic effects, threat-dependent issues, and retrofitting. These recommendations might help researchers or designers realize a more reliable and realistic progressive collapse design of RC frame structures in the future.

Bioinformatics Analysis Reveals Connection of Squamous Cell Carcinoma and Adenocarcinoma of the Lung

Fan, Wei-Dong,Zhang, Xian-Quan,Guo, Hui-Lin,Zeng, Wei-Wei,Zhang, Ni,Wan, Qian-Qian,Xie, Wen-Yao,Cao, Jin,Xu, Chang-Hua Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

Squamous cell carcinoma and adenocarcinoma are the major histological types of non-small cell lung cancer. Because they differ on the basis of histopathological and clinical characteristics and their relationship with smoking, their etiologies may be different; for example, different tumor suppressor genes may be related to the genesis of each type. We used microarray data to construct three regulatory networks to identify potential genes related to lung adenocarcinoma and squamous cell carcinoma and investigated the similarity and specificity of them. In the network, some of the observed transcription factors and target genes had been previously proven to be related to lung adenocarcinoma and squamous cell carcinoma. We also found some new transcription factors and target genes related to SCC. The results demonstrated that regulatory network analysis is useful in connection analysis between lung adenocarcinoma and squamous cell carcinoma.

Bio-Regulator Alter Root Architecture of Malus Hupehensis under Different Phosphate Condition

Wei-Guo Fan,Hong-Qiang Yang 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Overexpression of Ornithine Decarboxylase Suppresses Thapsigargin-Induced Apoptosis

Wei-Chung Hsieh,Pei-Chen Hsu,Ya-Fan Liao,Shu-Ting Young,Zeng-Wei Wang,Chih-Li Lin,Gregory J. Tsay,Huei Lee,Hui-Chih Hung,Guang-Yaw Liu 한국분자세포생물학회 2010 Molecules and cells Vol.30 No.4

Ornithine decarboxylase (ODC), the key enzyme of poly-amine biosynthesis, has paradoxical roles in apoptosis. Our published papers show overexpression of ODC pre-vents the apoptosis induced by many cytotoxic drugs. Thapsigargin (TG) is an inhibitor of the sarcoplasmic/en-doplasmic reticulum (ER) Ca2+ ATPase (SERCA) pumps and causes ER stress-induced apoptosis. We used ODC overexpressing cell lines to examine whether overexpres-sion of ODC inhibits TG-induced apoptosis. Our results indicated overexpression of ODC attenuated TG-induced apoptosis. Overexpression of ODC blocked procaspse-4 cleavage and phosphorylation of protein kinase-like ER-resident kinase (PERK), triggered by TG. It also attenuated the increase in CAAT/enhancer binding protein homolo-gous protein (CHOP). Cells with overexpressed ODC had greater Bcl-2 expression. Overexpression of ODC pre-served the expression of Bcl-2, inhibited the increase in Bak and stabilized mitochondrial membrane potential without the influences of TG. Cytochrome c release and downstream caspase activation were blocked. That is, overexpression of ODC inhibits the mitochondria-medi-ated apoptotic pathway, induced by TG. Finally, overex-pression of ODC maintains the protein and mRNA expres-sion of SERCA. In conclusion, overexpression of ODC suppresses TG-induced apoptosis by blocking caspase-4 activation and PERK phosphorylation, attenuating CHOP expression and inhibiting the mitochondria-mediated apoptotic pathway.

Biomechanical Comparison of Nucleotomy with Lumbar Spine Fusion versus Nucleotomy Alone: Vibration Analysis of the Adjacent Spinal Segments

Wei Fan,Li-Xin Guo 한국정밀공학회 2018 International Journal of Precision Engineering and Vol.19 No.10

This study aimed to investigate the effect of transforaminal lumbar interbody fusion (TLIF) with bilateral pedicle screw fixation (BPSF) on dynamic responses of the adjacent spinal segments to whole body vibration (WBV) after nucleotomy. A previously validated finite element model of an intact L1-sacrum lumbar spine was modified to simulate nucleotomy with and without TLIF and BPSF at L4-L5. Transit dynamic analyses were conducted on the nucleotomy alone and the fusion models under a vertical vibration load. The computed dynamic responses for the two models at adjacent levels were recorded and compared. The results showed that at level (L5-S1) below the denucleated disc, maximum response values of the disc bulge, annulus stress and intradiscal pressure decreased due to the fusion by 5.6%, 5.2% and 7.2%, and their vibration amplitudes decreased by 30.5%, 25.7% and 24.3%. At levels (L1-L2, L2-L3 and L3-L4) above the denucleated disc, maximum response values and vibration amplitudes of the strains and stresses also produced 5.2-8.9% and 25.9-29.7% deceases due to the fusion. It implies that after nucleotomy, application of the TLIF with BPSF might be helpful to prevent negative effects of the vertical WBV on adjacent disc levels.

Multicentre Hospital-based Case-control Study of Diffuse Large B-cell Lymphoma in Shanghai, China

Fan, Rong,Zhang, Lu-Yao,Wang, Hong,Yang, Bo,Han, Tao,Zhao, Xiao-Li,Wang, Wei,Wang, Xiao-Qin,Lin, Guo-Wei Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7

Background: Several potential risk factors have been identified for diffuse large B-cell lymphoma (DLBCL); however, epidemiological studies investigating the association between these risk factors and DLBCL have yielded inconsistent results. Objectives: To investigate potential medical, lifestyle, and environmental risk factors of DLBCL in Shanghai, China through a hospital-based case-control study. Method: One-hundred-and-forty-seven newly diagnosed DLBCL patients and 294 sex- and age-matched controls were recruited from 11 hospitals in Shanghai between 2003 and 2007. A standardized structured questionnaire was used to obtain patient data on demographics, medical history, family history, lifestyle, and environmental exposures. Conditional logistic regression models were used to estimate odds ratios (ORs), with 95% confidence intervals (CIs), for risk associated with each data category. Results: History of tuberculosis (TB) infection and "living on a farm" were positively associated with DLBCL (TB: OR=3.05, 95% CI: 1.19-7.80; farm: OR=1.82, 95% CI: 1.21-2.73). In contrast, taking traditional Chinese medicine was negatively associated with DLBCL (OR=0.36, 95% CI: 0.14-0.89). No significant correlation with DLBCL risk was found for any of the other potential risk factors (p>0.05), including but not limited to hair dyes, alcohol drinking, smoking, and home/workplace renovation within one year. Conclusions: Consistent with results from previous studies in other DLBCL case populations, traditional Chinese medicine appeared to have a direct or indirect protective effect against DLBCL. However, this study also identified a possible predisposition for DLBCL in TB sufferers and farmers.

De novo assembly and transcriptome analysis of differentially expressed genes relevant to variegation in hawthorn flowers

Wei Ji,Wei Zhao,Rong‑Chen Liu,Xiao‑Bo Jiao,Kai Han,Zhong‑Yi Yang,Mei‑Ying Gao,Rui Ren,Xiu‑Juan Fan,Ming‑Xia Yang 한국식물생명공학회 2019 Plant biotechnology reports Vol.13 No.6

Flower color variegation has been observed in many plant species. However, pink flowers on the white-blooming hawthorn trees found by our group earlier have never been reported. To better understand the differentially expressed genes (DEGs) in variegated hawthorn flowers, white and pink flowers at different developmental stages (S1 and S2) underwent transcriptome sequencing separately. Approximately 34.28 Gb of high-quality data were obtained and assembled into 100,013 unigenes with an average length of 706.93 bp. These unigenes were further subjected to functional annotation and biochemical pathway analysis, and DEGs of two types of hawthorn flowers at different developmental stages were studied. Based on the enrichment analysis of DEGs, eight anthocyanin-modified enzyme genes or other enzyme genes that indirectly affect anthocyanin synthesis (5AT, 3GGT , and AI, β-Glu, two Aux/IAAs, two PODs), eight structural genes (UFGT, DFR, CHI, two F3Hs, and three PALs), and three transcription factors (one MYB and two bHLHs) were also identified. We randomly selected 15 genes, and the trends in the expression levels of these genes in the organs of white and pink flowers at different developmental stages were verified by quantitative real-time PCR. Mass sequence data obtained by RNA-seq of variegated hawthorn flowers provided basic sequence information and a unique opportunity to uncover the genetic mechanisms under-lying flower color variegation.

A transcription factor TaTCP20 regulates the expression of Ppd-D1b in common wheat

Wei Fan,Song Tianqi,Zhou Jianfei,Cheng Jie,Li Ruibo,Yu Ming,Zhang Yunrui,Yu-Yang Song,Zhang Bo,Zhang Xiaoke 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.3

Photoperiod (Ppd) genes play an important role in the adaptation of wheat to the ecological environment. However, the transcriptional regulation mechanism of photoperiodic genes has remained elusive. This study isolated a full-length promoter of Ppd-D1b (2518 bp) from the common wheat genome. Several essential core cis-acting elements and numerous light-responsive cis-acting regulatory elements were identifed in Ppd-D1b promoter by the in-silico analysis. Ten 5’-deleted length fragments of the Ppd-D1b promoter fused with GUS were constructed and named D0 ~D9, then transferred them into Arabidopsis thaliana. GUS gene driven by full-length (D0) in transgenic Arabidopsis thaliana showed the same rhythm with Ppd-D1b in wheat under short-day conditions (SDs, 8-h light/16-h dark). The expression of GUS gene in D0 reached its peak at 3 h after dawn, then decreased to the lowest and remained stable. Analysis of the series of 5’-deleted fragments showed that at 3 h after dawn, GUS gene expression activity decreased signifcantly in D7a due to removal of CHEBS (CCA1 HIKING EXPEDITION binding site). Moreover, yeast one-hybrid (Y1H) and dual-luciferase (dual-LUC) assays revealed that TaTCP20 could bind to the Ppd-D1b promoter to increase its transcriptional activity. This study revealed a transcription factor, TaTCP20, which activated Ppd-D1b by binding to CHEBS, provided a foundation for the theoretical research on wheat’s photoperiodic response mechanism.

A PROSPECT TO THE DEVELOPMENT OF MYCOLOGY IN CHINA

( Wei Fan Chiu ) 한국균학회 1995 95 SECOND JOINT SEIMINAR Vol.- No.-